Biofouling最新文献

{"title":"Sequential attachment and <i>Listeria</i> predominance under turbulent flow.","authors":"Krisha Pant, Jon Palmer, Steve Flint","doi":"10.1080/08927014.2026.2630941","DOIUrl":"10.1080/08927014.2026.2630941","url":null,"abstract":"<p><p>The exploration of multispecies biofilms has provided significant insight into how bacteria interact in natural environments. However, crucial information is still lacking when the assumption that all the bacteria approach the substrate simultaneously during multispecies biofilm formation is challenged. The objective of this study was to analyse the multispecies biofilm formed by industrially relevant bacteria, including <i>Pseudomonas fluorescens</i> and <i>Listeria monocytogenes</i>, focusing on sequential colonisation under turbulent flow and static conditions. Under flow conditions, the attachment of <i>Listeria</i> on preformed <i>Pseudomonas</i> biofilm was significantly higher (<i>p</i> < 0.001) and reached its highest cell concentration (8.9 log CFUcm^-2) earlier (by 24 h) compared to control (sterile stainless-steel surfaces) and co-inoculated (with <i>P. fluorescens</i>) conditions. The conditioning of stainless-steel surfaces with freeze-dried and rehydrated exopolysaccharides (0-27.5 µgml^-1) extracted from <i>P. fluorescens</i> did not affect <i>L. monocytogenes</i> attachment, indicating the importance of biofilm architecture. The cell concentration of <i>P. fluorescens</i> was not affected by either the flow rate or the colonisation order in a multispecies biofilm. Overall, the sequence of colonisation affects biofilm formation in multispecies biofilms under turbulent flow and is an important variable in studies of the community lifestyle of multispecies biofilms.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"375-390"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting resistant <i>Staphylococcus aureus</i> biofilm with organic acids: uncovering the biofilm disinfection mechanism.","authors":"Iris Urbančič, Manca Lunder, Rok Fink","doi":"10.1080/08927014.2026.2634396","DOIUrl":"10.1080/08927014.2026.2634396","url":null,"abstract":"<p><p>This study examines the effects of ascorbic, acetic, citric, and lactic acids on resistant <i>S. aureus</i>, assessing planktonic growth using minimal inhibitory (MIC) and bactericidal concentrations, and evaluating mature biofilms for viability, biomass, enzyme activity, membrane integrity and stress. We found the lowest antimicrobial potential for acetic acid, followed by ascorbic acid, citric acid and lactic acid. Treatment of mature biofilms showed a reduction of up to 3 log CFU mL^-1 for lactic acid, while other organic acids were less effective. Lactic acid was also the most effective in reducing biofilm biomass by up to 33%, indicating potential for combination with other antibacterial compounds. The crystal violet staining confirmed a reduction in biomass regarding the non-treated samples. Iodonitrotetrazolium chloride assay showed a decrease in metabolic activity, with the highest formazan reduction (88%) observed with acetic acid. Live/dead staining indicated increased cell death at higher concentrations (9 MIC), with lactic acid causing the most severe membrane damage. In addition, intracellular stress increased with acid concentration. These findings reveal not only differential biofilm-targeting effects among common organic acids but also highlight the translational potential of lactic and acetic acids as cost-effective strategies to control resistant <i>S. aureus</i> in clinical and industrial settings, providing a foundation for future therapeutic and preventive applications.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"446-458"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative microparticle strategies: harnessing essential oil-loaded lecithin/chitosan systems against <i>Burkholderia pseudomallei</i>.","authors":"Anderson da Cunha Costa, Dayana Pinto de Meneses, Maria Rosiene Antunes Arcanjo, Mirele Rodrigues Fernandes, Augusto Feynman Dias Nobre, Alanna Mayara Soares de Sousa, Rosemayre Souza Freire, João Victor Serra Nunes, Thomas Crouzier, Ulrike Schimpf, Rodrigo Silveira Vieira, Raimunda Sâmia Nogueira Brilhante","doi":"10.1080/08927014.2026.2629008","DOIUrl":"10.1080/08927014.2026.2629008","url":null,"abstract":"<p><p><i>Burkholderia pseudomallei</i>, the causative agent of melioidosis, presents high antimicrobial resistance, largely due to its biofilm-forming ability. Here, innovative lecithin/chitosan microparticles loaded with lemongrass (LEO) and geranium (GEO) essential oils (LEOLCN and GEOLCN) were developed and characterized as a novel strategy to combat <i>B. pseudomallei</i>. Microparticles were characterized and evaluated for their effects on planktonic growth and biofilm inhibition, as well as for potential synergistic interactions with antibiotics. Microparticles exhibited a spherical morphology, high encapsulation efficiency, and sustained release profiles, with up to 45% of EO released at pH 2.0 and 32% at pH 7.4 after 50 h. LEOLCN demonstrated MICs ranging from 64 to 128 µgml^-1 for most strains, although some resistant isolates exhibited MICs of up to 2,048 µgml^-1. At a concentration of 512 µgml^-1, both LEOLCN and GEOLCN reduced biofilm biomass and metabolic activity by more than 50%. LEOLCN exhibited synergistic effects with conventional antibiotics, reducing ceftazidime MICs by 2- to 9-fold, suggesting its potential to enhance antibiotic therapy against resistant strains. Scanning electron microscopy confirmed biofilm disruption. Overall, the dual antimicrobial and antibiofilm action of these compounds introduces a promising and innovative <i>in vitro</i> platform to address the persistent challenge of <i>B. pseudomallei</i> infections and biofilm-associated resistance.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"361-374"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crevice corrosion of CuNi 90/10 under simulated macrofouling in temperate and tropical Australian marine environments.","authors":"M A Javed, R Piola, W C Neil, S A Wade","doi":"10.1080/08927014.2026.2630944","DOIUrl":"10.1080/08927014.2026.2630944","url":null,"abstract":"<p><p>Copper-nickel 90/10 alloys are widely used in marine environments due to their resistance to corrosion and biofouling. However, the influence of macrofouling on localised corrosion remains poorly understood. This study examines the effect of simulated macrofouling-induced crevices on CuNi 90/10 corrosion using laser-cut plastic shims to replicate hard fouling geometries. Samples with crevice gaps of 50, 130 and 1,000 µm were exposed for 180 days at two Australian marine sites: temperate (Melbourne) and tropical (Cairns). Crevice corrosion was most severe in Melbourne, with the 130 µm gap showing the deepest attack. In Cairns, samples showed lower crevice corrosion despite heavier external fouling, suggesting that fouling composition and protective calcareous deposits may mitigate attack. Crevice interiors lacked Desulfobacterota and exhibited less diverse communities with greater inter-replicate variability at both sites than outside surfaces. These results highlight the combined influence of environment, crevice geometry, corrosion product films, and biofouling composition on crevice corrosion of CuNi 90/10.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"391-410"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147301667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myricetin as a potential therapeutic agent against <i>Pseudomonas aeruginosa</i>: inhibition of biofilm formation, quorum sensing, and virulence factor production.","authors":"Sallah A Al Hashedi, Saif M Dmour, Hamad Sarheed Galut, Ayman Daifallah Ismail Alsheikh, Mohammad H Abukhalil, Sham Ma'aitah, Najeeb S Al-Zoreky, Khaled M A Ramadan, Muhammad Naeem Sattar, Zafar Iqbal, Adil Alshoaibi, Sultan A M Saghir","doi":"10.1080/08927014.2026.2634395","DOIUrl":"10.1080/08927014.2026.2634395","url":null,"abstract":"<p><p>Multidrug-resistant pathogenic bacteria, particularly <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>), pose a significant threat to human health. Despite the huge persistence of antibiotics, there remains a lack of effective natural compounds capable of simultaneously disrupting quorum sensing (QS), biofilm formation, and virulence in this pathogen. This study aimed to investigate the inhibitory potential of myricetin against <i>P. aeruginosa</i> focusing on its ability to interfere with QS-regulated virulence traits. Antibacterial, antibiofilm, anti-QS, and virulence factor activities were evaluated using crystal violet biofilm formation and QS-regulated virulence factor inhibition assays (e.g. pyocyanin, rhamnolipid, protease, and exopolysaccharides). The minimum inhibitory concentration (MIC) required to inhibit visible bacterial growth was 0.97 mg/mL. Additionally, the minimum biofilm inhibitory concentration of 50 (MBIC₅₀) was recorded at the MIC value. Myricetin showed a significant inhibitory effect against biofilm formation by suppressing bacterial hydrophobicity, aggregation, and swarming motility. Furthermore, myricetin significantly reduced the production of pyocyanin, rhamnolipid, protease, and exopolysaccharides. The myricetin effectively impeded QS mechanisms as evidenced by a significant reduction in the production of acyl homoserine lactone and violacein pigment, both qualitatively and quantitatively. Gene expression analysis exhibited a significant downregulation of <i>LasI</i>/<i>R</i> and <i>RhlI</i>/<i>R</i> genes, further enhancing the myricetin role in QS inhibition. Collectively, these findings demonstrate that myricetin effectively interferes with QS-mediated virulence mechanisms in <i>P. aeruginosa</i>, supporting its potential as a promising lead compound for developing anti-virulence strategies.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"429-445"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of β-lactam resistance in dual-species biofilms of <i>Pseudomonas aeruginosa</i> and methicillin-sensitive <i>Staphylococcus aureus</i>.","authors":"Waleska S da Cruz Nizer, Kira N Allison, Carole Beaulieu, Megan C Montgomery, Edana Cassol, Joerg Overhage","doi":"10.1080/08927014.2026.2620072","DOIUrl":"10.1080/08927014.2026.2620072","url":null,"abstract":"<p><p>Polymicrobial biofilms are frequently associated with chronic infections and are highly tolerant to antibiotic treatment. Given that β-lactam antibiotics are the most prescribed antibiotics worldwide, we investigated β-lactam resistance in dual-species biofilms formed by <i>Pseudomonas aeruginosa</i> PAO1 and methicillin-sensitive <i>Staphylococcus aureus</i> (MSSA). Compared to monocultures, MSSA exhibited reduced susceptibility to cephalexin, ampicillin, and cefazolin, while <i>P. aeruginosa</i> showed reduced susceptibility to cefazolin and nafcillin within dual-species biofilms. Using a set of <i>P. aeruginosa</i> mutants, we demonstrate that the PAO1 AmpC β-lactamase, the exopolysaccharides Psl and Pel, and the quorum sensing regulators LasR and RhlR each play significant roles in protecting MSSA from β-lactam treatment. Interestingly, co-cultures of MSSA with the Δ<i>pslA pelF</i> mutant strain increased the survival of Δ<i>pslA pelF</i> under β-lactam exposure. Overall, these findings advance our understanding of how interdependent bacterial interactions compensate for the loss of matrix components and thereby contribute to antimicrobial tolerance in polymicrobial biofilms.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"278-293"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146059325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of location and coating on the development of an in-water grooming program to maintain ship hull coatings.","authors":"Melissa Tribou, Geoffrey Swain, Harrison Gardner, Sam Royster, Jarema M Didoszak, Dean Webster, Joseph Dahlgren","doi":"10.1080/08927014.2026.2621192","DOIUrl":"10.1080/08927014.2026.2621192","url":null,"abstract":"<p><p>Ship hull grooming, a form of proactive in-water cleaning, has been extensively researched in a warm, productive, subtropical Atlantic location using US Navy-approved commercial coatings. Less is known about how grooming performs on toughened fouling control coatings or the grooming frequencies required in locations with lower seawater temperatures and lower fouling intensities. The purpose of this study was to explore differences in proactive in-water cleaning schedules due to changes in location and coating types. This study compared the time-based frequency of in-water grooming of two commercial and two experimental fouling control coatings in Port Canaveral, Florida, and Monterey, California. The results demonstrated that a higher grooming frequency was required to maintain surfaces free of fouling at the warm-water Port Canaveral, FL site compared to the cooler-water site in Monterey, CA. They also showed that the type of fouling control coating will influence the grooming frequency.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"305-323"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of capsaicin in antifouling technologies in marine environments.","authors":"Huanchao Shen, Mulan Mu, Zhiwei Jiao","doi":"10.1080/08927014.2026.2628690","DOIUrl":"10.1080/08927014.2026.2628690","url":null,"abstract":"<p><p>Marine biofouling has been negatively affecting human sailing since ancient times. Traditional antifouling coatings, such as tributyl tin-based coatings, were banned globally in 2008 due to their inherent toxicity and severe threat to the marine environment. Developing environmentally friendly, long-term marine antifouling coatings remains a huge challenge for the maritime industry. Capsaicin is one of the natural product antifoulants (NPAs). Capsaicin is favored by researchers due to its good antifouling effect and low toxicity. This review provides insights into four methods of application of capsaicin in the field of antifouling: incorporation into coatings, encapsulation <i>via</i> microcapsules, synthesis and exploration of capsaicin derivatives and incorporation into membranes to enhance the antifouling properties of materials. This paper compares the experimental data of various applied methods in the hope of enlightening interested researchers.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"233-249"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146211931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/08927014.2026.2626152","DOIUrl":"10.1080/08927014.2026.2626152","url":null,"abstract":"","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"338"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro performance of eugenol and cinnamaldehyde on biofilms produced by <i>Candidozyma auris</i>.","authors":"Débora Silva Marques de Sousa, Gabrielle Pires de Morais Monari, Cassiana Ferreira da Rosa, Ana Flávia Marques Pereira, Tatiane Baptista Zapata, Cauê Bastos Tertuliano Dos Santos, Ary Fernandes Júnior, Sandra de Moraes Gimenes Bosco, Marcia de Souza Carvalho Melhem, Luciana da Silva Ruiz","doi":"10.1080/08927014.2026.2613950","DOIUrl":"10.1080/08927014.2026.2613950","url":null,"abstract":"<p><p><i>Candidozyma auris</i> has high rates of resistance to antifungals and hospital disinfectants. This yeast remains on surfaces for long periods, favoring the colonization of patients. Similarly <i>Candida</i> species, it produces persistent biofilms, which contribute to invasive infections, resulting in high mortality rates. In the search for new therapeutic alternatives of antifungal action, natural products have been widely studied due to their pharmacological potential and diverse biological activities. Eugenol and cinnamaldehyde are derived from the essential oils of cinnamon and clove, respectively, and have shown action against several microorganisms, including <i>Candida</i> species. The aims of the present study were to evaluate the antibiofilm activity of the natural products eugenol and cinnamaldehyde on clinical isolates of <i>C. auris</i>. Both products showed inhibitory action on planktonic form. Biofilm biomass was considerably reduced for most of the evaluated isolates. On the other hand, during the treatment period, the metabolic activity of most isolates increased, compared to control, possibly due to the heteroresistance phenomenon; however, further studies are still needed to support this hypothesis.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"250-263"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}