BiofoulingPub Date : 2026-03-01Epub Date: 2026-02-02DOI: 10.1080/08927014.2026.2621190
Md Ramim Tanver Rahman, Ismail Fliss, Eric Biron
{"title":"Prevention of <i>Staphylococcus aureus</i> biofilm formation on catheters, contact lenses, and contact lens cases by a synthetic analogue of the antimicrobial lipopeptide humimycin.","authors":"Md Ramim Tanver Rahman, Ismail Fliss, Eric Biron","doi":"10.1080/08927014.2026.2621190","DOIUrl":"10.1080/08927014.2026.2621190","url":null,"abstract":"<p><p>Biofilm-associated infections, particularly those caused by <i>Staphylococcus aureus</i>, pose a persistent challenge in clinical settings and in medical devices such as catheters, wound dressings, orthopaedic and dental implants, and contact lenses. The resilience of biofilms to conventional antimicrobial treatments underscores the need for innovative strategies. This study evaluated the efficacy of a synthetic analogue of the antimicrobial lipopeptide humimycin as a preventive measure against biofilm formation of <i>S. aureus</i> on catheters, contact lenses and contact lens cases, both as a coating agent and in combination with commercial multipurpose contact lens solutions. The results demonstrate a dose-dependent reduction in biofilm formation with the humimycin analogue coatings achieving up to 98% inhibition at 256 µg/mL. This significant reduction was accompanied by a marked decrease in bacterial metabolic activity, indicating that the humimycin analogue disrupts biofilm integrity and impairs bacterial function. When combined with commercial contact lens solutions such as Opti-Free<sup>®</sup> Express<sup>®</sup> and Solocare Aqua<sup>®</sup>, the biofilm-disrupting activity of the lipopeptide was further enhanced, with lower minimum inhibitory concentration and minimum biofilm inhibitory concentration values. These findings suggest that the humimycin analogue holds significant promise as a novel antimicrobial strategy for preventing <i>S. aureus</i> biofilm-related infections acquired from medical devices.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"294-304"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2026-03-01Epub Date: 2026-01-19DOI: 10.1080/08927014.2026.2615081
Francisca Massano, Ana C Afonso, Mariana Sousa, Lília S Teixeira, Anabela Borges, Manuel Simões
{"title":"The action of selected monoterpenes as biofilm control agents and antibiotic resistance modifiers.","authors":"Francisca Massano, Ana C Afonso, Mariana Sousa, Lília S Teixeira, Anabela Borges, Manuel Simões","doi":"10.1080/08927014.2026.2615081","DOIUrl":"10.1080/08927014.2026.2615081","url":null,"abstract":"<p><p>Multidrug-resistant bacteria in biofilms are a growing public health threat, due to their resistance to conventional antibiotics. Phytochemicals are attractive candidates because of their structural diversity and ability to potentiate antimicrobial activity. This study investigated the antibiofilm and resistance-modifying effects of two monoterpenes, menthol and linalool, alone and in combination with ten antibiotics, against <i>Escherichia coli</i> and <i>Staphylococcus epidermidis</i>. Menthol exhibited MIC and MBC of 800 µg/mL against <i>E. coli</i> and the same MIC against <i>S. epidermidis</i>, while linalool showed MICs of 800 µg/mL and 400 µg/mL, respectively. Combination assays revealed enhanced activity of erythromycin with both monoterpenes against <i>E. coli</i> and of amoxicillin with menthol against <i>S. epidermidis</i>, although sessile cells were largely unaffected. When applied individually, both monoterpenes caused a 3-log reduction in culturable <i>E. coli</i> biofilm cells. The overall findings highlight the antibiofilm activity of linalool and, particularly, menthol, supporting their role as antibiotic adjuvants against biofilm-associated infections.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"264-277"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2026-03-01Epub Date: 2026-02-17DOI: 10.1080/08927014.2026.2625113
Hyeonwoo Choi, Hyeok Kim, Hyun-Suk Oh, Heekyong Oh
{"title":"Application of trivalent cation cross-linked quorum quenching beads for biofouling mitigation and phosphorus removal in a membrane bioreactor.","authors":"Hyeonwoo Choi, Hyeok Kim, Hyun-Suk Oh, Heekyong Oh","doi":"10.1080/08927014.2026.2625113","DOIUrl":"10.1080/08927014.2026.2625113","url":null,"abstract":"<p><p>This study applied quorum-quenching cell-entrapping beads (QQ-CEB) to mitigate membrane fouling and enhance phosphorus removal in a membrane bioreactor (MBR). Instead of suspending QQ-CEB in the MBR, immobilising it on a recovery net maintained the effluent total phosphorus removal at approximately 50%. It extended the system's operational period by about 2.2 times compared to a conventional MBR. Membrane fouling mitigation was attributed to quorum-quenching activity, reducing microbial products accumulated on the membrane surface by 28-47%. Signalling molecule analysis further confirmed that the QQ mechanism alleviated biofouling by inhibiting microbial quorum sensing. The Fe³<sup>+</sup>-cross-linked QQ-CEB exhibited an operational lifespan 1.53 times longer than that of the Ca<sup>2+</sup>-cross-linked QQ-CEB in the MBR process. Microbial community analysis of the Fe³<sup>+</sup> QQ-CEB indicated that the intrusion of external microorganisms was effectively suppressed, thereby maintaining a stable microbial community within the beads.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"324-337"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146211955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2026-02-01Epub Date: 2025-12-19DOI: 10.1080/08927014.2025.2605127
Cecília Rocha da Silva, João Batista de Andrade Neto, Vitória Pessoa de Farias Cabral, Érica Rayanne Mota da Costa, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Thais Lima Ferreira, Maria Janielly Castelo Branco Silveira, Elaine Aires de Lima, Ana Carolina Medeiros de Oliveira, Wildson Max Barbosa da Silva, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Lívia Gurgel do Amaral Valente Sá
{"title":"Preventive activity of promethazine against fluconazole-resistant <i>Candida</i> spp. biofilms fixed on urinary catheters.","authors":"Cecília Rocha da Silva, João Batista de Andrade Neto, Vitória Pessoa de Farias Cabral, Érica Rayanne Mota da Costa, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Thais Lima Ferreira, Maria Janielly Castelo Branco Silveira, Elaine Aires de Lima, Ana Carolina Medeiros de Oliveira, Wildson Max Barbosa da Silva, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Lívia Gurgel do Amaral Valente Sá","doi":"10.1080/08927014.2025.2605127","DOIUrl":"10.1080/08927014.2025.2605127","url":null,"abstract":"<p><p>Catheter-associated urinary tract infections (CA-UTI) are among the most prevalent infections, affecting over 150 million individuals worldwide annually. <i>Candida</i> is the most common fungal genus causing urinary infections, representing a significant medical challenge, particularly in patients undergoing prolonged catheterization-a risk factor that exacerbates clinical conditions by promoting the formation of resistant biofilms. In this context, the coating of catheters with antimicrobial substances combined with drug repositioning is an innovative alternative. In this study, we evaluated the activity of promethazine (PMT), a first-generation antihistamine, against the formation of <i>Candida</i> spp. biofilms on urinary catheter fragments. Broth microdilution, impregnation of urinary catheter fragments, and scanning electron microscopy (SEM) were the exploratory techniques employed. PMT proved to be an effective agent in inhibiting biofilm formation, achieving a 50% reduction in colony-forming units (CFU/mL). The results indicated that PMT exhibits high potential as an antifungal agent, with promising applications in coatings aimed at preventing <i>Candida</i> spp. infections associated with long-term urinary devices.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"148-157"},"PeriodicalIF":2.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145793160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The bio-corrosion mechanisms, mathematical modelling and machine learning applications in concrete structures: a systematic review.","authors":"Chenglong Yu, Xiaojie Chen, Zhihao Feng, Hui Rong, Xiaofeng Zhou","doi":"10.1080/08927014.2025.2597855","DOIUrl":"10.1080/08927014.2025.2597855","url":null,"abstract":"<p><p>Concrete is a widely utilised material for infrastructure such as sea-crossing bridges, harbour terminals and sewage pipelines. However, concrete structures are susceptible to various forms of deterioration, including biological and chemical corrosion. Among these, bio-corrosion poses a significant threat to structural durability by promoting surface scaling and inducing complex physicochemical and biophysical damage. This study provides a comprehensive review of the current state of bio-corrosion research, with a focus on clarifying its mechanisms and established quantification techniques. Additionally, it examines advances in mathematical modelling and machine learning for analysing bio-corrosion in concrete structures. This review also addresses ongoing debates and insights within the field of bio-corrosion and bio-protection.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"99-121"},"PeriodicalIF":2.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145970472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2026-02-01Epub Date: 2026-01-04DOI: 10.1080/08927014.2025.2610350
Lu Zhao, Linyang Wang, Dan Zhang, Donghe Tan, Meng Hao, Yufang Guo, Jiande Li, Xuan Ma, Shuge Tian
{"title":"Combining GC-MS, network pharmacology, and molecular docking to explore the biofilm inhibition and antibacterial effects of <i>Hyssopus cuspidatus</i> Boriss essential oil on <i>Staphylococcus aureus</i> ATCC 6538.","authors":"Lu Zhao, Linyang Wang, Dan Zhang, Donghe Tan, Meng Hao, Yufang Guo, Jiande Li, Xuan Ma, Shuge Tian","doi":"10.1080/08927014.2025.2610350","DOIUrl":"10.1080/08927014.2025.2610350","url":null,"abstract":"<p><p>The essential oil of <i>Hyssopus cuspidatus</i> Boriss (HC B) exhibits diverse pharmacological properties, yet its antibacterial mechanisms remain underexplored. This study identified 59 compounds, predominantly ketones, <i>via</i> GC-MS analysis, and demonstrated potent anti-<i>Staphylococcus aureus</i> (<i>S. aureus</i>) activity with an MIC of 7.8125 mg/mL and an MBC of 62.5 mg/mL. The essential oil significantly suppressed acid production, lactate dehydrogenase (LDH) activity, and biofilm formation in <i>S. aureus</i>. Crystal violet staining confirmed the disruption of preformed biofilms at MIC concentrations. Through molecular docking and kinetic simulations, key bioactive components (e.g. α-gurjunene, cuminal, terpineol) were predicted to target inflammation and oxidative stress-related genes (PTGS2, MAOA/B, RELA, HSP90AA1), suggesting a multimodal antibacterial mechanism. These findings reveal the novel potential of HCB essential oil as a natural anti-biofilm and anti-virulence agent against <i>S. aureus</i>.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"204-223"},"PeriodicalIF":2.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2026-02-01Epub Date: 2026-01-19DOI: 10.1080/08927014.2025.2608665
Anirban Ray, Swachchha Majumdar, Sourja Ghosh
{"title":"Development of surface-engineered super-hydrophobic antifouling membrane based microalgal photobioreactor for enhancing CO<sub>2</sub> sequestration and biomass productivity.","authors":"Anirban Ray, Swachchha Majumdar, Sourja Ghosh","doi":"10.1080/08927014.2025.2608665","DOIUrl":"10.1080/08927014.2025.2608665","url":null,"abstract":"<p><p>Escalating the efficacy of microalgal cultivation process is proposed in photobioreactor by using an innovative membrane-based bubble sparger for efficient dissolution of gas phase CO<sub>2</sub> using <i>Scenedesmus</i> sp. Surface functionalization was done on tubular ceramic substrates using Hexadecyltrimethylsiloxane (HDTMS) for enhancing mass transfer and antifouling property. The optimum membrane was found to be super-hydrophobic, contact angle >153°. The membrane sparger (HMS) based system resulted in higher biomass concentration (2.26 g/l) and higher CO<sub>2</sub> bio-fixation efficiency (0.57 g CO<sub>2</sub>/l/day) after seven days compared to 0.69 g/l and 0.15 g CO<sub>2</sub>/l/day for the conventional bubble sparger (CBS) system. Moreover, for HMS system biomass concentration and CO<sub>2</sub> bio-fixation efficiency increases up to 3.12 g/l and 0.80 g CO<sub>2</sub>/l/day respectively using dairy wastewater (50%). The overall study demonstrates the applicability of proposed process for enhancing <i>Scenedesmus</i> sp. biomass productivity and CO<sub>2</sub> sequestration in membrane photobioreactor (C-MPBR) process using industrial waste resources along with minimizing microalgae induced biofouling of the spargers.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"158-174"},"PeriodicalIF":2.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacterial-derived γ-aminobutyric acid (GABA) regulates larval settlement and metamorphosis of <i>Mytilus coruscus</i>.","authors":"Xiuping Yang, Xiaoyu Wang, Lihua Peng, Sergey Dobretsov, Jin-Long Yang, Xiao Liang","doi":"10.1080/08927014.2025.2603451","DOIUrl":"10.1080/08927014.2025.2603451","url":null,"abstract":"<p><p>Bacteria play a vital role in the settlement and metamorphosis of marine invertebrates. However, the molecular mechanisms by which bacteria regulate the metamorphosis from larva to juvenile remain unknown. This study aims to investigate whether bacteria derived from <i>Mytilus coruscus</i> affect the larval settlement and metamorphosis of this species. Our results showed that all eight bacterial strains can induce settlement and metamorphosis in this mussel species. Polysaccharides and gamma-aminobutyric acid (GABA) produced by bacteria positively trigger this transformation in mussels, with GABA acting as the primary inducer. <i>Marinomonas foliarum</i> exhibited the highest GABA production and induction activity among the experimental strains. Compared with biofilms alone, the addition of a GABA synthesis inhibitor during <i>Marinomonas foliarum</i> biofilm formation reduced its induction activity by 56.02%. Our results reveal a novel role of bacterial GABA in triggering mussel larval settlement and metamorphosis.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"136-147"},"PeriodicalIF":2.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2026-02-01Epub Date: 2026-01-15DOI: 10.1080/08927014.2025.2595131
Brittney Mitchell, Melissa Chernick, Daniel Rittschof, Nishad Jayasundara
{"title":"Embryonic exposure to irgarol impacts development and larval and juvenile swimming in zebrafish (<i>Danio rerio</i>).","authors":"Brittney Mitchell, Melissa Chernick, Daniel Rittschof, Nishad Jayasundara","doi":"10.1080/08927014.2025.2595131","DOIUrl":"10.1080/08927014.2025.2595131","url":null,"abstract":"<p><p>The primary method for mitigating the growth of biofouling organisms on submerged surfaces is through the use of broad-spectrum biocides, such as irgarol, which can impact non-target species. We assessed the developmental and bioenergetic impacts of early-life-stage irgarol exposure on zebrafish (<i>Danio rerio</i>), including impacts on survival, hatching, developmental abnormalities, heart rate, mitochondrial function, and behaviour. We exposed zebrafish embryos 6-144 h post-fertilisation (hpf) to irgarol at concentrations currently detected in the environment and at pharmacological concentrations. At environmental concentrations, irgarol did not impact survival, hatching, morphological development or mitochondrial function. However, significant changes to heart rate and swimming behaviour occurred. Larvae were significantly hyperactive following exposure, then hypoactive after being grown in clean conditions for weeks. Our study showed that while irgarol is not acutely toxic at environmental levels, it has sublethal impacts on cardiac and neurological functions that could result in decreased fish fitness. The risks that irgarol presents to food security, environmental health, and human health warrant study.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"122-135"},"PeriodicalIF":2.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145970488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2026-02-01Epub Date: 2026-01-15DOI: 10.1080/08927014.2025.2610308
J Anani, K Dam-Johansen, J A H Dreyer
{"title":"Automated underwater imaging system for continuous biofouling assessment.","authors":"J Anani, K Dam-Johansen, J A H Dreyer","doi":"10.1080/08927014.2025.2610308","DOIUrl":"10.1080/08927014.2025.2610308","url":null,"abstract":"<p><p>Traditional antifouling coating evaluations rely on manual sample retrieval, imaging and subjective visual assessments, which are labour-intensive and constrained by infrequent sampling intervals that may overlook critical fouling developments. This study presents a novel, automated biofouling assessment system at the CoaST Maritime Test Centre. A custom underwater camera captures daily images, while advanced image analysis techniques track fouling progression. To address imaging challenges, a median stacking technique removes transient artefacts such as overhanging algae and floating debris, enabling accurate assessment of attachment points, the primary metric for biofouling coverage. Multi-exposure fusion enhances image illumination. For image analysis, <i>ilastik</i>, a machine-learning tool using a random forest algorithm, performs pixel classification segmentation to differentiate biofouling classes and monitor their growth. This automated approach improves accuracy, repeatability and efficiency in biofouling assessment, reducing subjectivity and enhancing antifouling research.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"175-189"},"PeriodicalIF":2.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145970504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}