{"title":"亚油酸和硬脂酸对紫色色杆菌外膜囊泡介导毒力的群体猝灭作用。","authors":"Venkatramanan Mahendrarajan, Nalini Easwaran","doi":"10.1080/08927014.2024.2446930","DOIUrl":null,"url":null,"abstract":"<p><p><i>Chromobacterium violaceum</i> is a pathogenic bacterium that can infect humans and animals, yet the role of its outer membrane vesicles (OMVs) in mediating pathogenicity remains underexplored. This study evaluated the effects of linoleic acid (LA) and stearic acid (SA) on quorum sensing (QS)-mediated violacein production, biofilm formation, and OMV biogenesis in <i>C. violaceum</i>. Our findings revealed that 2 mM LA and 1 mM SA effectively quench QS, leading to a significant reduction in violacein production, biofilm formation, and OMV biogenesis. Gene expression analysis confirmed the downregulation of QS-related genes, including <i>cviI</i>, <i>cviR</i>, <i>vioA</i>, <i>vioB</i>, and <i>vioC</i>, in fatty acid-treated <i>C. violaceum</i>. Additionally, we assessed the antimicrobial activity of <i>C. violaceum</i>-derived OMVs on <i>Rhizobium sp.</i>, a PGPR and observed a marked reduction in bactericidal activity in the treated OMVs. This study suggests that LA and SA have potential as anti-infective agents to mitigate OMV-mediated virulence and combat antibiotic resistance in pathogens.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-11"},"PeriodicalIF":2.6000,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quorum quenching effects of linoleic and stearic acids on outer membrane vesicle-mediated virulence in <i>Chromobacterium violaceum</i>.\",\"authors\":\"Venkatramanan Mahendrarajan, Nalini Easwaran\",\"doi\":\"10.1080/08927014.2024.2446930\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Chromobacterium violaceum</i> is a pathogenic bacterium that can infect humans and animals, yet the role of its outer membrane vesicles (OMVs) in mediating pathogenicity remains underexplored. This study evaluated the effects of linoleic acid (LA) and stearic acid (SA) on quorum sensing (QS)-mediated violacein production, biofilm formation, and OMV biogenesis in <i>C. violaceum</i>. Our findings revealed that 2 mM LA and 1 mM SA effectively quench QS, leading to a significant reduction in violacein production, biofilm formation, and OMV biogenesis. Gene expression analysis confirmed the downregulation of QS-related genes, including <i>cviI</i>, <i>cviR</i>, <i>vioA</i>, <i>vioB</i>, and <i>vioC</i>, in fatty acid-treated <i>C. violaceum</i>. Additionally, we assessed the antimicrobial activity of <i>C. violaceum</i>-derived OMVs on <i>Rhizobium sp.</i>, a PGPR and observed a marked reduction in bactericidal activity in the treated OMVs. This study suggests that LA and SA have potential as anti-infective agents to mitigate OMV-mediated virulence and combat antibiotic resistance in pathogens.</p>\",\"PeriodicalId\":8898,\"journal\":{\"name\":\"Biofouling\",\"volume\":\" \",\"pages\":\"1-11\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-12-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biofouling\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/08927014.2024.2446930\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofouling","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/08927014.2024.2446930","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
紫色杆菌是一种可以感染人和动物的致病性细菌，但其外膜囊泡（OMVs）在介导致病性中的作用尚不清楚。本研究评价了亚油酸（LA）和硬脂酸（SA）对群体感应（QS）介导的堇菜素生产、生物膜形成和OMV生物发生的影响。我们的研究结果表明，2 mM LA和1 mM SA有效地抑制了QS，导致紫罗兰素的产生、生物膜的形成和OMV的生物发生显著减少。基因表达分析证实，脂肪酸处理的紫葡萄中，qs相关基因cviI、cviR、vioA、vioB和vioC下调。此外，我们还评估了堇菜衍生的omv对根瘤菌（Rhizobium sp.，一种PGPR）的抑菌活性，发现处理过的omv的抑菌活性明显降低。这项研究表明，LA和SA有潜力作为抗感染药物，减轻omv介导的毒力和对抗病原体的抗生素耐药性。
Quorum quenching effects of linoleic and stearic acids on outer membrane vesicle-mediated virulence in Chromobacterium violaceum.
Chromobacterium violaceum is a pathogenic bacterium that can infect humans and animals, yet the role of its outer membrane vesicles (OMVs) in mediating pathogenicity remains underexplored. This study evaluated the effects of linoleic acid (LA) and stearic acid (SA) on quorum sensing (QS)-mediated violacein production, biofilm formation, and OMV biogenesis in C. violaceum. Our findings revealed that 2 mM LA and 1 mM SA effectively quench QS, leading to a significant reduction in violacein production, biofilm formation, and OMV biogenesis. Gene expression analysis confirmed the downregulation of QS-related genes, including cviI, cviR, vioA, vioB, and vioC, in fatty acid-treated C. violaceum. Additionally, we assessed the antimicrobial activity of C. violaceum-derived OMVs on Rhizobium sp., a PGPR and observed a marked reduction in bactericidal activity in the treated OMVs. This study suggests that LA and SA have potential as anti-infective agents to mitigate OMV-mediated virulence and combat antibiotic resistance in pathogens.
期刊介绍：
Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion.
Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context.
Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
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