IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-15 DOI: 10.1080/08927014.2026.2747767

Nga Phuong Dang, Tabassum Ali, Vasco C Mota, Carlo C Lazado, Peter Szabor

{"title":"Assessment of disinfectant performance against fish-pathogenic bacteria in planktonic and biofilm states.","authors":"Nga Phuong Dang, Tabassum Ali, Vasco C Mota, Carlo C Lazado, Peter Szabor","doi":"10.1080/08927014.2026.2747767","DOIUrl":"https://doi.org/10.1080/08927014.2026.2747767","url":null,"abstract":"Disinfecting fish farming equipment and facility surfaces with disinfectant is one of the primary biosafety measures to prevent disease introduction and transmission. However, the actual efficacy of approved disinfectants against fish-pathogenic bacteria and especially their biofilms remain largely undocumented. Six selected disinfectants were evaluated against seven common disease-causing bacteria and their biofilms. None of the tested disinfectants exhibited good efficacy against Mycobacterium salmoniphilum (the log reductions were ≤ 3.3). Two disinfectants showed low efficacy against Pseudomonas fluorescens in comparison to other tested bacteria, log reduction ≤ 4. Among three bacterial biofilms, good efficacies were only observed against the biofilm of Aliivibrio wodanis with 100% biofilm viability reduction for all disinfectants. Reduced disinfectant efficacy was observed for the biofilms of Yersinia ruckeri (97.4 - 100%) and P. fluorescens (> 99%). The study underscores significant gaps in disinfectant performance against fish pathogens, particularly Gram-positive species and suggests that bacterial biofilm formation can substantially compromise disinfection efficacy.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-16"},"PeriodicalIF":2.5,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149179247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing the antifouling efficacy of UVC used in conjunction with marine coatings for two static immersion test sites on the east coast of Florida. 在佛罗里达东海岸的两个静态浸泡试验点比较UVC与船舶涂料结合使用的防污效果。

IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-05 DOI: 10.1080/08927014.2026.2736784

Tenzin Yeshi, Kelli Hunsucker, Madison Kozee, Abigail Cermak, Geoff Swain, Hank Lobe, Scott Mangum, Sara Tuck, Kenan Fears

{"title":"Comparing the antifouling efficacy of UVC used in conjunction with marine coatings for two static immersion test sites on the east coast of Florida.","authors":"Tenzin Yeshi, Kelli Hunsucker, Madison Kozee, Abigail Cermak, Geoff Swain, Hank Lobe, Scott Mangum, Sara Tuck, Kenan Fears","doi":"10.1080/08927014.2026.2736784","DOIUrl":"https://doi.org/10.1080/08927014.2026.2736784","url":null,"abstract":"Biofouling is a major issue in the marine and shipping industry. Marine coatings have been predominantly used in its management. Ultraviolet-C (UVC) treatment has been gaining interest as an antifouling method and recently has been under consideration for use in conjunction with coatings. A two-part study was conducted to investigate the efficacy of using UVC in preventing biofouling on ship hull coatings. The first part performed a test site comparison of UVC's efficacy in synergy with marine coatings for three months between an estuarine and an oceanic environment. The turbid, estuarine environment showed a higher abundance of biofouling compared to the oceanic site. Location factors like temperature and turbidity determine the biofouling rate and composition. Turbidity attenuates UVC transmission and affects its performance. Results demonstrate the importance of location-specific regime in terms of UVC duty cycling and coating compatibility in the environment of operation. The second part performed a long-term assessment of UVC with the same marine coatings at the oceanic site. After nine months of immersion, the results showed that UVC was effective at preventing macro-fouling on four different marine coatings. A chemical analysis of coatings post UVC exposure in the field environments demonstrated that silicone coatings were the most susceptible to chemical induced changes from UVC, but in general, all coatings had minimal impacts to the surface chemistry. Here, UVC, combined with marine coatings, has proven to be an effective fouling control tool in low turbid waters across a larger temporal scale.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-13"},"PeriodicalIF":2.5,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm-associated microbial risks in a mega water diversion project: distribution of putative pathogen-associated taxa and concrete biocorrosion potential in the Middle Route canal of the South-to-North water diversion project. 大型调水工程生物膜相关微生物风险：南水北调中线水渠推定病原相关类群分布及具体生物腐蚀潜势

IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-05 DOI: 10.1080/08927014.2026.2742367

Shun Huang, Yifan Tang, Zhicong Wang, Dunhai Li

{"title":"Biofilm-associated microbial risks in a mega water diversion project: distribution of putative pathogen-associated taxa and concrete biocorrosion potential in the Middle Route canal of the South-to-North water diversion project.","authors":"Shun Huang, Yifan Tang, Zhicong Wang, Dunhai Li","doi":"10.1080/08927014.2026.2742367","DOIUrl":"https://doi.org/10.1080/08927014.2026.2742367","url":null,"abstract":"The ecological assembly of putative pathogen-associated taxa and the biogeochemical potential related to concrete biocorrosion have rarely been examined within an integrated framework in large freshwater diversion systems. We conducted quarterly biofilm sampling at eight stations along the Middle Route canal of the South-to-North Water Diversion Project, and combined 16S rRNA gene amplicon sequencing with shotgun metagenomics to characterize longitudinal and seasonal microbial patterns. Taxonomy-based screening identified 279 putative pathogen-associated ASVs with a mean relative abundance of 3.40%, primarily affiliated with Bacillus and Brevundimonas. Their relative abundance was lowest in the middle reaches, where higher flow velocity and dissolved oxygen may reduce biofilm-associated retention. Total nitrogen accounted for the largest individual contribution among the measured environmental variables (6.13%), whereas normalized stochasticity ratios indicated that stochastic processes predominated in overall community assembly. Metagenomic analysis further revealed spatially structured nitrogen- and sulfur-cycling potential, including biocorrosion-associated taxa such as Thiobacillus and Desulfovibrio, with several related functional pathways showing comparatively higher abundances in upstream biofilms. These findings establish an integrated ecological framework in which stochastic assembly, nutrient-associated selection, and hydrodynamic modulation jointly shape biofilm-associated microbial risks. The study provides critical insights for safeguarding both water-quality monitoring and century-scale infrastructure performance in mega water diversion systems.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-16"},"PeriodicalIF":2.5,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen-mediated biofilm formation and fibrillar organization in Staphylococcus epidermidis. 表皮葡萄球菌胶原介导的生物膜形成和纤维组织。

IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-02 DOI: 10.1080/08927014.2026.2740722

Chisato Takahashi

{"title":"Collagen-mediated biofilm formation and fibrillar organization in Staphylococcus epidermidis.","authors":"Chisato Takahashi","doi":"10.1080/08927014.2026.2740722","DOIUrl":"https://doi.org/10.1080/08927014.2026.2740722","url":null,"abstract":"The symbiotic skin bacterium Staphylococcus epidermidis moisturizes human skin, contributes to protection against external pathogens, and supports innate immune stimulation. However, the interaction between collagen, a major skin protein, and S. epidermidis remains insufficiently explored. In this study, biofilm development was investigated using scanning electron microscopy with ionic liquid pretreatment, confocal laser scanning microscopy, biofilm quantification assays, and growth curve analysis. In the presence of collagen, cells adhered to the substrate, secreted microvesicles, formed web-like intercellular fibrillar connections, and became embedded in an extracellular polymeric substance-like matrix. Confocal microscopy and quantitative analyses showed that collagen supplementation significantly increased biofilm thickness and biomass over time, with biomass approximately 1.6-fold higher at 36 h, while growth curves indicated minimal effects on bacterial proliferation. As biofilms matured, surface cells exhibited organized alignment, and thread-like fibrils connected cells in less dense regions. These findings suggest that collagen promotes distinct biofilm architectures and influences the structural development of biofilms.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-17"},"PeriodicalIF":2.5,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the functional involvement of CALM and ATP2B in 5-hydroxytryptamine-induced larval metamorphosis in Mytilus coruscus. 评价CALM和ATP2B在5-羟色胺诱导的贻贝幼虫变态中的功能参与。

IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-02 DOI: 10.1080/08927014.2026.2734109

Changyu Guo, Jiajie Xu, Xiuping Yang, Deborah M Power, Xiao Liang, Lihua Peng, Jin-Long Yang

{"title":"Evaluating the functional involvement of CALM and ATP2B in 5-hydroxytryptamine-induced larval metamorphosis in Mytilus coruscus.","authors":"Changyu Guo, Jiajie Xu, Xiuping Yang, Deborah M Power, Xiao Liang, Lihua Peng, Jin-Long Yang","doi":"10.1080/08927014.2026.2734109","DOIUrl":"https://doi.org/10.1080/08927014.2026.2734109","url":null,"abstract":"Larval settlement and metamorphosis are critical developmental transitions in the macrofouling organisms, directly impacting population recruitment. Although 5-hydroxytryptamine (5-HT) effectively induces these processes, the downstream molecular mechanisms, particularly the role of intracellular Ca2+ homeostasis, in larval settlement and metamorphosis of Mytilus coruscus remain poorly understood. In this study, we investigated the roles of the calmodulin gene (CALM) and the plasma membrane Ca2+-ATPase gene (ATP2B), in 5-HT induced larval settlement and metamorphosis. Compared to pediveliger larvae, the expression of both CALM and ATP2B showed significant differences after metamorphosis. In 5-HT exposed larvae, CALM and ATP2B were up-regulated by 41.63% and 33.52%, respectively, whereas a CALM knockdown subsequently down-regulated CALM and up-regulated ATP2B. Knockdown of the CALM gene significantly inhibited the inductive effects of 5-HT on larval settlement and metamorphosis, with a similar reduction by ATP2B knockdown. The 5-HT-induced Ca2+ fluctuations were resolved over time in ATP2B-deficient larvae, but persisted in CALM-deficient larvae. Collectively, our findings identify CALM as the key mediator of 5-HT-induced larval metamorphosis by reciprocally regulating ATP2B and Ca2+ homeostasis. This knowledge of larval metamorphosis regulation is crucial for explaining macrofouling settlement and for advancing biofouling control technologies by providing new targets.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and evaluation of a dynamic in vitro biofilm model of dental unit waterlines incorporating simulated back-suction. 含模拟反吸的牙单元水线动态体外生物膜模型的开发与评价。

IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-01 DOI: 10.1080/08927014.2026.2736045

Ting Shuai, Shurui Xue, Dan Li, Xin Zuo, Wenjing Liu, Yanhui Lu, Xiue Li

{"title":"Development and evaluation of a dynamic in vitro biofilm model of dental unit waterlines incorporating simulated back-suction.","authors":"Ting Shuai, Shurui Xue, Dan Li, Xin Zuo, Wenjing Liu, Yanhui Lu, Xiue Li","doi":"10.1080/08927014.2026.2736045","DOIUrl":"https://doi.org/10.1080/08927014.2026.2736045","url":null,"abstract":"This study developed a dynamic in vitro dental unit waterlines (DUWLs) biofilm model incorporating simulated back-suction. The model consisted of 165-cm polyurethane tubing connected to a dynamic flow system and operated intermittently to simulate routine clinical use. Simulated back-suction was produced by injecting 0.1 mL of clinical oral fluid into the tubing after each operating cycle. Over 6 weeks, three model waterlines with simulated back-suction and three without simulated back-suction were compared with three clinical DUWLs from dental chair units. Planktonic bacterial counts in outlet water were measured longitudinally, and biofilm formation on the inner tubing surface was evaluated by heterotrophic plate counts and scanning electron microscopy at predefined time points. Outlet bacterial counts in clinical and back-suction model groups both exceeded 100 CFU/mL from day 3, with parallel trajectories (p > 0.05). In contrast, the model without back-suction exceeded the threshold only after day 9, with significantly lower counts. Biofilms in the back-suction model developed into dense, multilayered structures, whereas only sparse microcolonies formed otherwise. These findings support the clinical relevance of incorporating simulated back-suction into an in vitro DUWL biofilm model and its potential use as a platform for future studies of DUWL biofilm formation and control.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-11"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifouling and fouling-release coatings with polysaccharides: a nature-based solution to a marine challenge. 含多糖的防污和防污涂料：一种以自然为基础的海洋挑战解决方案。

IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-01 Epub Date: 2026-09-03 DOI: 10.1080/08927014.2026.2725688

Anand Lopez, Russell C Wyeth, Shegufa Shetranjiwalla

{"title":"Antifouling and fouling-release coatings with polysaccharides: a nature-based solution to a marine challenge.","authors":"Anand Lopez, Russell C Wyeth, Shegufa Shetranjiwalla","doi":"10.1080/08927014.2026.2725688","DOIUrl":"10.1080/08927014.2026.2725688","url":null,"abstract":"Marine biofouling presents unique challenges in various industrial applications. This has historically been addressed using antifouling or fouling-release coatings. However, issues with the leaching of active or non-active components with non-target toxicity have resulted in increased regulatory scrutiny of these solutions. Natural polysaccharide-based coatings have emerged as an alternative as they contain many chemical functional groups that can enhance fouling-release/antifouling properties. In this review, polysaccharide structural properties, such as crystallinity and ionic character, are discussed with respect to functional coatings properties, such as wettability and antifouling performance. The limitations of polysaccharide antifouling performance in environments of high silt and salt are also noted. Mitigation of these challenges by hydrophobic chemical modifications - amphiphilic polysaccharides - are explored as the frontier for high-performance coatings development. Recommendations are provided for future coatings development to better reflect real-world conditions, including using polysaccharides in commercial resin technologies and more extensive field test validation.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"942-961"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正。

IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-01 Epub Date: 2026-10-14 DOI: 10.1080/08927014.2026.2749891

引用次数: 0

Design and fabrication of microstructured PDMS surfaces for antifouling and drag reduction. 用于防污和减阻的微结构PDMS表面的设计和制造。

IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-01 Epub Date: 2026-09-22 DOI: 10.1080/08927014.2026.2734113

Qinsong Zhu, Chen Zhang, Shoulv Xin

{"title":"Design and fabrication of microstructured PDMS surfaces for antifouling and drag reduction.","authors":"Qinsong Zhu, Chen Zhang, Shoulv Xin","doi":"10.1080/08927014.2026.2734113","DOIUrl":"10.1080/08927014.2026.2734113","url":null,"abstract":"Marine biofouling on ship hulls substantially increases hydrodynamic drag and operational costs. This study selects the barnacle, a sessile crustacean with strong adhesion ability, and develops polydimethylsiloxane films patterned with micro-cylinders to achieve dual-functional antifouling and drag reduction based on the barnacle larvae dimensions and hydrophobic principle. Bioassays with barnacle cyprid larvae demonstrated that the microstructured surfaces significantly inhibited settlement, achieving an antifouling rate of 59%. Rotational drag tests further revealed a maximum drag reduction of 7.2% at 100 rpm. To elucidate the physical mechanisms, computational fluid dynamics simulations using the volume of fluid model were conducted. The numerical results verified the stability of the entrapped gas-liquid interface within the microstructures and revealed a substantial reduction in near-wall turbulence intensity and shear stress, quantitatively validating the slip-effect mechanism. These combined experimental and numerical results demonstrate the potential of engineered microstructured surfaces for marine antifouling and drag reduction applications.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1003-1026"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro anti-cyanobacterial activity of copper roof granules towards Gloeocapsa alpicola. 铜顶板颗粒体外抗蓝细菌活性研究。

IF 2.5 3区生物学

Biofouling Pub Date : 2026-10-01 Epub Date: 2026-09-01 DOI: 10.1080/08927014.2026.2723291

N Yasoubi, Valerie C A Ward

{"title":"In vitro anti-cyanobacterial activity of copper roof granules towards Gloeocapsa alpicola.","authors":"N Yasoubi, Valerie C A Ward","doi":"10.1080/08927014.2026.2723291","DOIUrl":"10.1080/08927014.2026.2723291","url":null,"abstract":"Gloeocapsa spp. are a type of airborne cyanobacteria that are known for causing black streaks on asphalt roofs. Embedding copper containing granules into asphalt shingles was developed to prevent cyanobacterial growth on roof shingles. There are currently no established methods for testing the anti-cyanobacterial activity of copper roofing granules. First, the minimum inhibitory concentration (MIC) of Cu2+ ions in solution was evaluated by monitoring growth in a 96 well plate over 14 days. Using a Gompertz function the MIC of Cu2+ was determined to be 0.592 ppm for G. alpicola. To test copper granules used in shingles, the granules were either autoclaved in BG11 media or sterilized using anhydrous ethanol, dried, and added to sterile BG11. Once inoculated with G. alpicola, growth was monitored using optical density (OD) and fluorescence measurements. Debris from the granules interfered with OD measurements but fluorescence clearly showed granules formulated with copper prevented cyanobacterial growth and those without did not. This work is of practical use for companies looking to formulate anti-cyanobacterial products like roof granules as it reports a specific MIC for this airborne species of cyanobacteria often found on roofs and also validates a methodology for testing roof granules.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"920-928"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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