IF 2.6 3区生物学

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-07-02 DOI: 10.1080/08927014.2025.2526210

Nirmeen Aboelnaga, Sama S Eltaher, Nehal A Saif, Omar Loay, Ahmed Abd El-Rahman, Abanoub Haroun, Clara Hakim, Manar Elsayed, Nayera E Attallah, Hossam B El-Geneidy, Maha Nasr, Mohamed Elhadidy

{"title":"Nano-boosted thymoquinone: moving beyond antibiotics to inhibit Staphylococcus aureus biofilms.","authors":"Nirmeen Aboelnaga, Sama S Eltaher, Nehal A Saif, Omar Loay, Ahmed Abd El-Rahman, Abanoub Haroun, Clara Hakim, Manar Elsayed, Nayera E Attallah, Hossam B El-Geneidy, Maha Nasr, Mohamed Elhadidy","doi":"10.1080/08927014.2025.2526210","DOIUrl":"10.1080/08927014.2025.2526210","url":null,"abstract":"Staphylococcus aureus is a high-priority pathogen requiring novel antimicrobial strategies. Thymoquinone (TQ), a bioactive compound from Nigella sativa, exhibits antimicrobial and antibiofilm properties, but its precise mechanisms remain unclear. This study evaluated free and nano-encapsulated TQ against multidrug-resistant S. aureus isolates. Nano-encapsulation enhanced biofilm penetration, with TQ significantly reducing extracellular DNA (eDNA) at sub-MIC levels without affecting initial adhesion, exopolysaccharides, or enzymatic virulence. Comparative analysis with vancomycin, ciprofloxacin, and azithromycin confirmed TQ's potent antibiofilm activity. Notably, TQ downregulated crtN, essential for staphyloxanthin biosynthesis, and msaB, a key biofilm and stress response regulator. However, at MIC and super-MIC levels, TQ paradoxically increased staphyloxanthin, suggesting a concentration-dependent oxidative stress response. We propose that TQ disrupts biofilm integrity by modulating the msaABCR operon. These findings highlight nano-TQ's therapeutic potential for biofilm-associated infections and underscore the role of natural compounds in combating multidrug-resistant pathogens.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"676-695"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Butenolide synergises with vancomycin to eradicate pre-formed biofilm of Staphylococcus aureus by interfering with energy-associated metabolism. 丁烯内酯与万古霉素协同作用，通过干扰能量相关代谢来根除金黄色葡萄球菌预先形成的生物膜。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-07-11 DOI: 10.1080/08927014.2025.2531136

Dai-Xin Hu, Jinyou Liang, Jing Yan, Zhi-Wen Ma, Hai-Lin Li, Ying Xu, Yu Zhang, Qi Yin

{"title":"Butenolide synergises with vancomycin to eradicate pre-formed biofilm of Staphylococcus aureus by interfering with energy-associated metabolism.","authors":"Dai-Xin Hu, Jinyou Liang, Jing Yan, Zhi-Wen Ma, Hai-Lin Li, Ying Xu, Yu Zhang, Qi Yin","doi":"10.1080/08927014.2025.2531136","DOIUrl":"10.1080/08927014.2025.2531136","url":null,"abstract":"Bacterial biofilms significantly contribute to persistent infections and the emergence of drug resistance of Staphylococcus aureus. Integrating conventional antibiotics with antibiofilm agents represents a promising strategy for combating biofilm-associated infections. This study systematically investigated the antibiofilm activity and underlying mechanisms of butenolide (BU) against methicillin-resistant S. aureus (MRSA), with a focus on the synergistic effects between BU and vancomycin (VAN). BU exhibited dual antibiofilm activities by efficiently preventing biofilm formation and eradicating established biofilms. Phenotypic characterisation revealed that 200 μg/mL of BU suppressed extracellular DNA production and autoaggregation of MRSA, leading to a significant reduction in biofilm thickness, biovolume, and coverage by up to 30%, 98%, and 96%, respectively. Transcriptome and quantitative-PCR analyses showed that BU treatment downregulated the expression of genes involved in energy metabolism. Notably, BU exhibited promising synergistic and additive effects with VAN in eradicating pre-formed biofilms, achieving synergy or additivity in five out of six S. aureus clinical strains tested, with a minimal fractional inhibitory concentration index as low as 0.375. These results highlight the potential of BU as an effective antibiofilm agent for preventing S. aureus-related infections.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"721-734"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the antibiofilm effect of nitrofurantoin and rutin in uropathogenic Klebsiella pneumoniae. 探讨呋喃妥因和芦丁对尿路致病性肺炎克雷伯菌的抗菌作用。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-07-17 DOI: 10.1080/08927014.2025.2532675

Seetha Lakshmi Rajangam, Kakithakara Vajravelu Leela, Manonmoney Jayaraman, Manoj Kumar Narasimhan

引用次数: 0

Suppressive effect of arginine and sodium fluoride on enamel calcium loss and antibiofilm activity on mature oral microcosm biofilms. 精氨酸和氟化钠对牙釉质钙流失和成熟口腔微生物膜抗生物膜活性的抑制作用。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-07-15 DOI: 10.1080/08927014.2025.2527776

Daniela Alejandra Cusicanqui Méndez, Maricel Rosário Cardenas Cuellar, Flaviana Bombarda de Andrade, Linda Wang, Marília Afonso Rabelo Buzalaf, Juliano Pelim Pessan, Thiago Cruvinel, Caio Sampaio

{"title":"Suppressive effect of arginine and sodium fluoride on enamel calcium loss and antibiofilm activity on mature oral microcosm biofilms.","authors":"Daniela Alejandra Cusicanqui Méndez, Maricel Rosário Cardenas Cuellar, Flaviana Bombarda de Andrade, Linda Wang, Marília Afonso Rabelo Buzalaf, Juliano Pelim Pessan, Thiago Cruvinel, Caio Sampaio","doi":"10.1080/08927014.2025.2527776","DOIUrl":"10.1080/08927014.2025.2527776","url":null,"abstract":"This study investigated the effects of arginine, with/without sodium fluoride (NaF), on mature oral microcosm biofilms and enamel calcium loss (ECL). Saliva-derived biofilms grown on bovine enamel for three days were subsequently assigned to their respective test groups and cultured until day seven (n = 9/group). Biofilm parameters and ECL were assessed. Reductions in the number of lactobacilli were observed with arginine at 2.5%/8% concentrations, with/without NaF, while 8% arginine, with/without NaF, reduced the number of all microorganism groups. The addition of 8% arginine and combinations of arginine with NaF reduced biofilm vitality. While all treatments resulted in a reduction of α-EPS, 8% arginine showed the most pronounced effect, being the only treatment to decrease β-EPS compared to the control. Reductions in ECL were achieved by 8% arginine, with/without NaF, 2.5% with NaF, and NaF, compared with the negative control (untreated biofilms). These findings demonstrated that arginine and NaF increased antibiofilm activity against mature biofilms, reducing ECL.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"696-710"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk of biocontamination of salted environments by P. aeruginosa and E. coli. 铜绿假单胞菌和大肠杆菌对盐渍环境的生物污染风险。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-07-17 DOI: 10.1080/08927014.2025.2529393

Mourad Elgoulli, Soukaina Mitro, Oubid Ait Lahbib, Ikhlas Hani Chennoufi, Hafida Zahir, Mostafa Ellouali, Hassan Latrache

{"title":"Risk of biocontamination of salted environments by P. aeruginosa and E. coli.","authors":"Mourad Elgoulli, Soukaina Mitro, Oubid Ait Lahbib, Ikhlas Hani Chennoufi, Hafida Zahir, Mostafa Ellouali, Hassan Latrache","doi":"10.1080/08927014.2025.2529393","DOIUrl":"10.1080/08927014.2025.2529393","url":null,"abstract":"Salty environments are susceptible to biological contamination by halotolerant microorganisms, by the phenotypic adaptation of microbial populations through the induction of survival mechanisms such as stress response pathways, and biofilm formation. Thus, this study aimed to investigate the bio-contamination risks posed by Pseudomonas aeruginosa and Escherichia coli in salted environments. The adhesion of the strains to glass and the surface energies were monitored in an aqueous medium at varying concentrations of sodium chloride (NaCl) (0%, 3%, 6%, 13%, 26%). Bacterial adhesion was observed by the optical microscopy, and the surface energies were estimated using the contact angle method. Surface energy measurements showed that NaCl was able to increase the electron donor (γ-) and acceptor (γ+) characters, and the hydrophobicity (ΔGiwi) of the bacterial surfaces at 3%. At 6%, 13%, and 26%, bacterial surfaces gradually regained their normal hydrophobicity. Similarly, the hydrophobicity of the glass surface increased and even reversed at 26%. The adhesion images showed an agglutination of the bacterial cells of both strains at 3% and 6%. However, at 6%, 13% and 26%, the adhesion becomes more dispersed and lighter. In brief, these findings suggest that NaCl may contribute to the enhancement of contamination in salted environments. Consequently, this raises concerns regarding the potential for bio-contamination in salty foods.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"711-720"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of oral microbiota and tooth loss in cardiovascular disease risk: a systematic review. 口腔微生物群和牙齿脱落在心血管疾病风险中的作用：一项系统综述

IF 2.6 3区生物学

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-06-23 DOI: 10.1080/08927014.2025.2518280

Pillar Gonçalves Pizziolo, Cláudia Helena Silva-Lovato, Lorena Mosconi Clemente, Angelica Carandina, Helio Cesar Salgado, Thais Marques da Silva, Eleonora Tobaldini, Nicola Montano, Adriana Barbosa Ribeiro

{"title":"The role of oral microbiota and tooth loss in cardiovascular disease risk: a systematic review.","authors":"Pillar Gonçalves Pizziolo, Cláudia Helena Silva-Lovato, Lorena Mosconi Clemente, Angelica Carandina, Helio Cesar Salgado, Thais Marques da Silva, Eleonora Tobaldini, Nicola Montano, Adriana Barbosa Ribeiro","doi":"10.1080/08927014.2025.2518280","DOIUrl":"10.1080/08927014.2025.2518280","url":null,"abstract":"This systematic review explores the relationship between the oral microbiome in edentulous patients and cardiovascular diseases (CVD). A search was conducted across databases PubMed, Scopus, Web of Science, Embase, and gray literature sources. The review followed PRISMA and AMSTAR guidelines. After removing duplicates, the articles were evaluated by titles and abstracts, and 21 were selected for full-text review, with 9 ultimately included in the final analysis. Oral dysbiosis, linked to systemic conditions like obesity, diabetes, and CVD, is common in inflammatory oral diseases such as periodontitis and denture stomatitis, disrupting original oral microbiota. This imbalance may lead to transient bacteremia and systemic inflammation, contributing to CVD development. Tooth loss reduces salivary microbiome diversity, and denture use in CVD patients is linked to decreased life expectancy compared to dentate individuals. The findings suggest tooth loss, denture use, and oral microbiota dysbiosis are unconventional risk factors for CVD progression.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"649-663"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Baicalein of Scutellaria baicalensis inhibits Mycobacterium abscessus biofilm formation to restore the antibiotics susceptibility. 黄芩黄芩素抑制脓肿分枝杆菌生物膜形成，恢复抗生素敏感性。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-07-15 DOI: 10.1080/08927014.2025.2531139

Xiaorui Zhang, Baoyu Dong, Qiurong He, Xingyan Tan, Yuqing Li, Kelei Zhao, Xinyue Xu, Jumei Zeng

{"title":"Baicalein of Scutellaria baicalensis inhibits Mycobacterium abscessus biofilm formation to restore the antibiotics susceptibility.","authors":"Xiaorui Zhang, Baoyu Dong, Qiurong He, Xingyan Tan, Yuqing Li, Kelei Zhao, Xinyue Xu, Jumei Zeng","doi":"10.1080/08927014.2025.2531139","DOIUrl":"10.1080/08927014.2025.2531139","url":null,"abstract":"Mycobacterium abscessus is resistant to traditional anti-TB medications and most currently available antibiotics, its strong biofilm formation may contribute to the resistance and make the situation even worse. In this study, Scutellaria baicalensis extract significantly inhibited the planktonic growth as well as the biofilm formation of M. abscessus. Baicalein, the principal active element in S. baicalensis extract, inhibited the biofilm formation instead of the planktonic growth and reduced extracellular matrix lipids in M. abscessus biofilm. The synergistic effects of baicalein in combination with clinical drugs were investigated, the baicalein effectively restored the susceptibility of M. abscessus to the investigated drugs in the biofilm growth. The transcriptome analysis revealed that 98 genes were upregulated and 19 genes were downregulated after baicalein treatment, genes involved in the fatty acid synthesis pathway were validated by RT-qPCR. Therefore, the extract of S. baicalensis and its monomer baicalein might serve as potential innovative adjuvant agents for the prevention and treatment of M. abscessus biofilm formation in chronic infections.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"735-750"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silver complex of 1,10-phenanthroline containing 2-formylpyridine-N(4)-ethylthiosemicarbazone: study of toxicity using alternative methods and microbiological activity against Helicobacter pylori. 含2-甲酰基吡啶- n(4)-乙基硫代氨基脲的1,10-菲罗啉银配合物：替代方法毒性研究及对幽门螺杆菌的微生物活性

IF 2.6 3区生物学

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-07-17 DOI: 10.1080/08927014.2025.2515922

Maíra Oliveira Dos Santos, Bruna Almeida Furquim de Camargo, Débora Eduarda Soares Silva, Gabriel Davi Marena, Jessyca Aparecida Paes Dutra, Tainá Grecco Dos Santos, Marlus Chorilli, Adelino Vieira de Godoy Netto, Taís Maria Bauab

{"title":"Silver complex of 1,10-phenanthroline containing 2-formylpyridine-N(4)-ethylthiosemicarbazone: study of toxicity using alternative methods and microbiological activity against Helicobacter pylori.","authors":"Maíra Oliveira Dos Santos, Bruna Almeida Furquim de Camargo, Débora Eduarda Soares Silva, Gabriel Davi Marena, Jessyca Aparecida Paes Dutra, Tainá Grecco Dos Santos, Marlus Chorilli, Adelino Vieira de Godoy Netto, Taís Maria Bauab","doi":"10.1080/08927014.2025.2515922","DOIUrl":"10.1080/08927014.2025.2515922","url":null,"abstract":"Helicobacter pylori is associated with gastric disorders, and the increase in bacterial resistance has sparked interest in the search for new antimicrobial compounds. The current study investigated the effect of the silver metal complex [Ag(phen)(PETSC)](NO3)∙HCl∙2H2O (phen = 1,10-phenanthroline; PETSC = 2-formylpyridine-N(4)-ethyl-thiosemicarbazone), termed the Compound against H. pylori. The antibacterial activity exhibited a minimum inhibitory concentration of 6.25 μg/mL. The checkerboard titration method assessed the combination of the Compound with amoxicillin and tetracycline, which presented antagonistic and indifferent effects, respectively. Time Kill kinetics were bactericidal after 12 h of treatment. Biofilm inhibition results showed antibacterial activity and bacterial morphological changes were observed by scanning electron microscopy. In vitro, cytotoxicity in normal gastric cells showed IC50 of 0.6733 μg/mL. The alternative in vivo test using Galleria mellonella showed acute toxicity at a dose of 150 mg/kg. Therefore, technological strategies can be an alternative to overcome the cytotoxicity since the Compound has a bactericidal effect against H. pylori.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"664-675"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dihydrochalcone derivatives as promising antifoulants: synthesis, bioactivity evaluation and performance in coatings. 二氢查尔酮类防污剂的合成、生物活性评价及其在涂料中的应用。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-07-22 DOI: 10.1080/08927014.2025.2535419

Daniela Pereira, Érica Lima, Danilo Correia, Vitor Vasconcelos, Madalena Pinto, Marta Correia-da-Silva, Joana R Almeida, Honorina Cidade

{"title":"Dihydrochalcone derivatives as promising antifoulants: synthesis, bioactivity evaluation and performance in coatings.","authors":"Daniela Pereira, Érica Lima, Danilo Correia, Vitor Vasconcelos, Madalena Pinto, Marta Correia-da-Silva, Joana R Almeida, Honorina Cidade","doi":"10.1080/08927014.2025.2535419","DOIUrl":"https://doi.org/10.1080/08927014.2025.2535419","url":null,"abstract":"Marine biofouling presents a major challenge for the maritime industry and marine ecosystems, traditionally managed through biocide-based antifouling (AF) coatings. However, the environmental toxicity of these biocides has intensified the search for sustainable, environmentally friendly alternatives. In this study, a novel approach using flavonoid-based compounds as environmentally safe AF agents is presented. Building on the previous identification of the prenylated dihydrochalcone (DH345P), the first reported dihydrochalcone with AF activity, here a series of dihydrochalcone analogues was synthesized and evaluated to further explore the structure-activity relationship studies (SAR). Among the compounds studied, dihydrochalcone (10) emerged as the most effective, exhibiting the best performance regarding anti-settlement activity (EC50 2.34 µM), while remaining non-toxic to A. salina. To assess real-world applicability, compound 10 was incorporated into marine polyurethane (PU)-based coatings, which resulted in significantly reduced mussel larvae adherence compared to blank control coatings. These findings highlight dihydrochalcone-based compounds as a promising scaffold for sustainable AF agents. Considering their AF potential, non-toxic profile and feasible synthesis, flavonoids such as compound 10 might be explored as an alternative for conventional AF biocides, paving the way for greener marine coatings.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-11"},"PeriodicalIF":2.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprospecting seaweed derived bio-oil as a marine biofouling mitigating agent. 海藻生物油作为海洋生物污染缓蚀剂的生物勘探。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-07-21 DOI: 10.1080/08927014.2025.2527774

Sainath Gopinathan, Srividhya Krishnan, Sowndarya Jothipandiyan, Subramaniyasharma Sivaraman, Lakkakula Satish, Ponnusami Venkatachalam, Saravanan Ramiah Shanmugam, Nithyanand Paramasivam

{"title":"Bioprospecting seaweed derived bio-oil as a marine biofouling mitigating agent.","authors":"Sainath Gopinathan, Srividhya Krishnan, Sowndarya Jothipandiyan, Subramaniyasharma Sivaraman, Lakkakula Satish, Ponnusami Venkatachalam, Saravanan Ramiah Shanmugam, Nithyanand Paramasivam","doi":"10.1080/08927014.2025.2527774","DOIUrl":"https://doi.org/10.1080/08927014.2025.2527774","url":null,"abstract":"Mitigating marine biofouling using marine resources has become a research hotspot as it is considered an environmentally friendly approach. Hence, this study investigated the biofilm mitigating property and antifouling activity of bio-oil extracted from the pyrolysis of seaweed biomass. The bio-oil inhibited up to 73-80% of biofilm and extracellular polymeric substance (EPS) formation of the predominant marine microfoulers Nitratireductor kimnyeongensis, Nitratireductor aquibiodomus and Stutzerimonas stutzeri. Gas chromatography-mass spectrometry (GC-MS) analysis of the bio-oil identified that 13-Docosenamide (Z) is a prominent compound that accounts for about 27.42% of the total bio-oil composition which might be responsible for its antibiofilm property. The bio-oil was further formulated into antifouling paint equivalent to the consistency of traditional antifouling paints and coated on titanium plates. The water contact angle results showed that bio-oil and antifouling paint exhibit hydrophilic surfaces, effectively reducing bacterial attachment. Scanning electron microscopic analysis revealed that the anti-fouling paint coated on titanium plates against mixed species of microfoulers significantly reduced biofilms. Molecular docking of 13-Docosenamide (Z) against the mussel adhesive foot protein of Perna viridis (Pvfp-5b) exhibited favorable binding scores, indicating that it may reduce the bio-adhesion of macrofoulers to the substrate.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-16"},"PeriodicalIF":2.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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