João Marcos Spessoto Pingueiro, Magda Feres, Tatiane Tiemi Macedo, Aline Paim de Abreu Paulo Gomes, Felipe Mazzini Silva Vilela, Manuela Rocha Bueno, Lucas Daylor Aguiar da Silva, Anna Paula de Souza Silva, Severino Matias Alencar, Pedro Luiz Rosalen, Bruno Bueno-Silva
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引用次数: 0
摘要
本研究的目的是评价新前庭醇-前庭醇组分（NVF）对离体牙龈下多物种生物膜的影响。使用卡尔加里装置形成33种生物膜7天。从第3天开始，每天两次，每次1分钟：NV(400-1,600µg ml-1)，氯己定0.12% （CHX；阳性对照）或对照物（阴性对照）。7天后，分别通过比色反应和DNA-DNA杂交测定代谢活性和微生物组成。采用方差分析/Tukey’s和Kruskal-Wallis/Dunn’s进行分析（p < p >牙周梭菌和牙龈卟啉单胞菌）。nv1600对多形核梭杆菌也有抑制作用。在牙科实践中，NV似乎是控制生物膜形成和致病性的良好候选者。
Combination of neovestitol and vestitol impair the subgingival multispecies biofilm development.
The aim of this study was to evaluate effects of neovestitol-vestitol fraction (NVF) on an in vitro subgingival multispecies biofilm. The 33-species biofilm was formed for seven days using a Calgary device. Starting on day 3, treatments for applied twice daily for 1 min each: NV (400-1,600 µgml-1), chlorhexidine 0.12% (CHX; positive control) or vehicle (negative control). After seven days, metabolic activity and microbial composition were accessed through colorimetric reaction and DNA-DNA hybridization, respectively. ANOVA/Tukey's and Kruskal-Wallis/Dunn's were performed (p < 0.05). NV1,600 and NV800 and CHX significantly reduced biofilm metabolic activity by 67%, 48% and 64% respectively, compared to vehicle-treatment. NV1,600, NV800 and CHX reduced red complex proportions versus vehicle-treatment. NV1,600 also reduced orange complex and increased healthy-associated purple complex compared to negative control (p < 0.05). NV1,600, NV800 and CHX reduced nine species, including Fusobacterium periodonticum and Porphyromonas gingivalis. NV1,600 also reduced Fusobacterium nucleatum polymorphum. NV seems to be a good candidate to control biofilm formation and pathogenicity in dental practice.
期刊介绍：
Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion.
Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context.
Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
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