BiofoulingPub Date : 2024-01-01Epub Date: 2024-02-19DOI: 10.1080/08927014.2024.2316611
Huan Jiang, Zhennan Wang, Ai-Qun Jia
{"title":"Methyl gallate from <i>Camellia nitidissima</i> Chi flowers reduces quorum sensing related virulence and biofilm formation against <i>Aeromonas hydrophila</i>.","authors":"Huan Jiang, Zhennan Wang, Ai-Qun Jia","doi":"10.1080/08927014.2024.2316611","DOIUrl":"10.1080/08927014.2024.2316611","url":null,"abstract":"<p><p><i>Aeromonas hydrophila</i>, a Gram-negative zoonotic bacterium, causes high mortality in fish farming and immunocompromised patients. This study aimed to extract methyl gallate (MG) from the flowers of <i>Camellia nitidissima</i> Chi and evaluate its potential as a quorum sensing inhibitor (QSI) against <i>Aeromonas hydrophila</i> SHAe 115. MG reduced QS-associated virulence factors, including hemolysis, protease, and lipase, while impairing swimming motility and biofilm formation. Additionally, MG down-regulated positive regulatory genes (<i>ahyR</i>, <i>fleQ</i>) and up-regulated negative regulators (<i>litR</i>, <i>fleN</i>). This highlights MG's promise as a potent QSI for <i>A. hydrophila</i> SHAe 115, advancing strategies against infections in aquaculture and human health.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"64-75"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139904886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2024-01-01Epub Date: 2024-02-14DOI: 10.1080/08927014.2024.2310482
Martina Oder, Kaća Piletić, Rok Fink, Zvonimir Marijanović, Romana Krištof, Lucija Bićanić, Dijana Tomić Linšak, Ivana Gobin
{"title":"A synergistic anti-bacterial and anti-adhesion activity of tea tree (<i>Melaleuca alternifolia</i>) and lemon eucalyptus tree (<i>Eucalyptus citriodora</i> Hook) essential oils on <i>Legionella pneumophila</i>.","authors":"Martina Oder, Kaća Piletić, Rok Fink, Zvonimir Marijanović, Romana Krištof, Lucija Bićanić, Dijana Tomić Linšak, Ivana Gobin","doi":"10.1080/08927014.2024.2310482","DOIUrl":"10.1080/08927014.2024.2310482","url":null,"abstract":"<p><p><i>Legionella pneumophila</i> is a Gram-negative bacterial pathogen that colonizes natural and artificial water systems and has the ability to form a biofilm. The biofilm protects <i>L. pneumophila</i> from various environmental factors and makes it more resistant to chlorine-based disinfectants. This study investigated the anti-bacterial properties of tea tree (<i>Melaleuca alternifolia</i> (Maiden and Betche) Cheel) oil and lemon eucalyptus tree (<i>Eucalyptus citriodora</i> Hook) essential oils (EOs) and their synergistic, additive inhibitory and anti-adhesive effects against <i>L. pneumophila</i> biofilm formation on polystyrene. The minimum effective concentration (MEC) for tea tree is 12.8 mg ml<sup>-1</sup> and for lemon eucalyptus tree EO 6.4 mg ml<sup>-1</sup>. In the checkerboard assay, different combinations of these two EO show synergistic and additive anti-microbial activity. The minimum anti-adhesive concentration (MAC) for tea tree is 12.8 mg ml<sup>-1</sup> and for lemon eucalyptus tree EO 6.4 mg ml<sup>-1</sup>. A combination of 3.2 mg ml<sup>-1</sup> tea tree EO and 0.8 mg ml<sup>-1</sup> lemon eucalyptus tree EO showed the strongest anti-adhesive effect against <i>L. pneumophila</i> on polystyrene. The tested oils and their combination showed intriguing potential to inhibit <i>L. pneumophila</i> biofilm formation.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"54-63"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139728850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>Cymbopogon citratus</i> L. essential oil as a potential anti-biofilm agent active against antibiotic-resistant bacteria isolated from chronic rhinosinusitis patients.","authors":"Mintra Khosakueng, Suwimol Taweechaisupapong, Wongwarut Boonyanugomol, Pornpan Prapatpong, Saharut Wongkaewkhiaw, Sakawrat Kanthawong","doi":"10.1080/08927014.2024.2305387","DOIUrl":"10.1080/08927014.2024.2305387","url":null,"abstract":"<p><p>Chronic rhinosinusitis (CRS) is long-term inflammation of the sinuses that can be caused by infection due to antibiotic-resistant bacteria. Biofilm developed by microbes is postulated to cause antibiotic treatment failure. Thus, the anti-biofilm activities of seven Thai herbal essential oils (EOs) against antibiotic-resistant bacteria isolated from CRS patients was investigated. Lemongrass (<i>Cymbopogon citratus</i> L.) EO showed the most effective antibiofilm activity against <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus epidermidis</i> grown as biofilm. GC-MS analysis found that myrcene was the major bioactive compound. Pretreatment with lemongrass EO significantly inhibited biofilm formation of all bacterial strains in more than 50% of cases. Furthermore, confocal microscopy analysis revealed the biofilm-disrupting activity of lemongrass EO against the biofilm matrix of all these bacterial species and also increased <i>P. aeruginosa</i> swarming motility with no toxicity to human cells. These results suggest that lemongrass EO has promising clinical applications as an anti-biofilm agent for CRS patients.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"26-39"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139575192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2024-01-01Epub Date: 2024-02-26DOI: 10.1080/08927014.2024.2320721
Camilla Malcher Pesset, Carolina O da Fonseca, Milena Antunes, Ana Luiza L Dos Santos, Izabel Melo Teixeira, Eliane de Oliveira Ferreira, Bruno Penna
{"title":"Biofilm formation by <i>Staphylococcus pseudintermedius</i> on titanium implants.","authors":"Camilla Malcher Pesset, Carolina O da Fonseca, Milena Antunes, Ana Luiza L Dos Santos, Izabel Melo Teixeira, Eliane de Oliveira Ferreira, Bruno Penna","doi":"10.1080/08927014.2024.2320721","DOIUrl":"10.1080/08927014.2024.2320721","url":null,"abstract":"<p><p>Osteomyelitis often involves <i>Staphylococcus</i> spp. as the isolated genus in domestic animal cases. Implant-related infections, frequently associated with biofilm-forming microorganisms like staphylococci species, necessitate careful material selection. This study assessed biofilm formation by <i>Staphylococcus pseudintermedius</i> on titanium nuts used in veterinary orthopaedic surgery. Biofilm quantification employed safranin staining and spectrophotometric measurement, while bacterial counts were determined in colony-forming units (CFU). Scanning Electron Microscopy (SEM) evaluated the biofilm morphology on the surface of titanium nuts. All samples had CFU counts. Absorbance values that evidence biofilm formation were observed in seven of the eight samples tested. SEM images revealed robust bacterial colonization, and significant extracellular polymeric substance production, and the negative control displayed surface irregularities on the nut. Whole genome sequencing revealed accessory Gene Regulator (<i>agr</i>) type III in six samples, <i>agr</i> IV and <i>agr</i> II in two each. Genes encoding <i>hlb</i>, <i>luk-S</i>, <i>luk-F</i>, <i>siet</i>, <i>se_int</i>, and the <i>icaADCB</i> operon were identified in all sequenced samples. Other exfoliative toxins were absent. Biofilm formation by <i>S. pseudintermedius</i> was detected in all samples, indicating the susceptibility of orthopaedic titanium alloys to adhesion and biofilm formation by veterinary species. The biofilm formation capacity raises concerns about potential post-surgical complications and associated costs.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"88-97"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139970892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2024-01-01Epub Date: 2024-01-22DOI: 10.1080/08927014.2024.2305385
Deisy Guimarães Carneiro, Ananda Pereira Aguilar, Hilário Cuquetto Mantovani, Tiago Antônio de Oliveira Mendes, Maria Cristina Dantas Vanetti
{"title":"The quorum sensing molecule C12-HSL promotes biofilm formation and increases <i>adrA</i> expression in <i>Salmonella</i> Enteritidis under anaerobic conditions.","authors":"Deisy Guimarães Carneiro, Ananda Pereira Aguilar, Hilário Cuquetto Mantovani, Tiago Antônio de Oliveira Mendes, Maria Cristina Dantas Vanetti","doi":"10.1080/08927014.2024.2305385","DOIUrl":"10.1080/08927014.2024.2305385","url":null,"abstract":"<p><p>Acyl-homoserine lactones (AHLs) are quorum-sensing signaling molecules in Gram-negative bacteria and positively regulate biofilm formation in <i>Salmonella</i> under specific conditions. In this study, biofilm formation in <i>Salmonella enterica</i> was evaluated at 28 and 37 °C, under aerobic and anaerobic conditions. Additionally, the influence of the <i>N</i>-dodecanoyl-DL-homoserine lactone (C12-HSL) on biofilm formation and the expression of genes related to the synthesis of structural components, regulation, and quorum sensing was assessed under anaerobiosis at 28 and 37 °C. Biofilm formation was found not to be influenced by the atmospheric conditions at 28 °C. However, it was reduced at 37 °C under anaerobiosis. C12-HSL enhanced biofilm formation at 37 °C under anaerobiosis and increased the expression of the <i>adrA</i> and <i>luxS</i> genes, suggesting an increase in c-di-GMP, a second messenger that controls essential physiological functions in bacteria. These results provide new insights into the regulation of biofilm formation in <i>Salmonella</i> under anaerobic conditions.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"14-25"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139519907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Food grade disinfectants based on hydrogen peroxide/peracetic acid and sodium hypochlorite interfere with the adhesion of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes to stainless steel of differing surface roughness","authors":"Ružica Tomičić, Zorica Tomičić, Milica Nićetin, Violeta Knežević, Sunčica Kocić-Tanackov, Peter Raspor","doi":"10.1080/08927014.2023.2288886","DOIUrl":"https://doi.org/10.1080/08927014.2023.2288886","url":null,"abstract":"This study aimed to evaluate the potential of the bacterium Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes to adhere to stainless steel discs with differ...","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":"79 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138568911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2023-12-11DOI: 10.1080/08927014.2023.2284316
Anusha Y. G., Lavanya Mulky
{"title":"Biofilms and beyond: a comprehensive review of the impact of Sulphate Reducing Bacteria on steel corrosion","authors":"Anusha Y. G., Lavanya Mulky","doi":"10.1080/08927014.2023.2284316","DOIUrl":"https://doi.org/10.1080/08927014.2023.2284316","url":null,"abstract":"Sulphate-reducing bacteria (SRB) are known to cause severe corrosion of steel structures in various industries, resulting in significant economic and environmental consequences. This review paper c...","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":"6 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138579354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2023-10-01Epub Date: 2023-12-04DOI: 10.1080/08927014.2023.2290120
Carolina Alves Freiria de Oliveira, Leonardo Guedes da Silva Moraes, Ana Beatriz Vilela Teixeira, Valéria Oliveira Pagnano
{"title":"Antimicrobial activity of cleansers on the cobalt-chromium surface of removable partial denture: a systematic review.","authors":"Carolina Alves Freiria de Oliveira, Leonardo Guedes da Silva Moraes, Ana Beatriz Vilela Teixeira, Valéria Oliveira Pagnano","doi":"10.1080/08927014.2023.2290120","DOIUrl":"10.1080/08927014.2023.2290120","url":null,"abstract":"<p><p>This study aimed to review systematically the literature about the antimicrobial action of evaluated cleansers on the Co-Cr alloy of RPD. The search was conducted in MEDLINE/PubMed, Scopus, Lilacs, Embase and Science Direct May, 2022. The review was performed based on PRISMA guidelines and recorded in Open Science Framework. Independent reviewers performed the search, selection, extraction, and analysis of the data. The risk of bias of the <i>in vitro</i> and clinical trials studies was analyzed by the Joanna Briggs Institute tool. A total of 187 articles were found and 9 were included. The cleansers that showed antimicrobial action were 2% and 5.25% sodium hypochlorite, 0.12% chlorhexidine and NitrAdine effervescent tablet. Polident, Corega Tabs effervescent tablets and 5 mg/mL chitosan solution showed intermediate effects. Propolis and green tea toothpaste were not effective. Three articles presented a high risk of bias and 6, low risk. The cleansers that showed the highest antimicrobial efficacy on Co-Cr alloy were 0.12% chlorhexidine digluconate and NitrAdine and can be safely used on RPD framework.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"916-927"},"PeriodicalIF":2.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138476683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofoulingPub Date : 2023-10-01Epub Date: 2023-12-18DOI: 10.1080/08927014.2023.2294763
Reetika Debroy, Sudha Ramaiah
{"title":"Consolidated knowledge-guided computational pipeline for therapeutic intervention against bacterial biofilms - a review.","authors":"Reetika Debroy, Sudha Ramaiah","doi":"10.1080/08927014.2023.2294763","DOIUrl":"10.1080/08927014.2023.2294763","url":null,"abstract":"<p><p>Biofilm-associated bacterial infections attributed to multifactorial antimicrobial resistance have caused worldwide challenges in formulating successful treatment strategies. In search of accelerated yet cost-effective therapeutics, several researchers have opted for bioinformatics-based protocols to systemize targeted therapies against biofilm-producing strains. The present review investigated the up-to-date computational databases and servers dedicated to anti-biofilm research to design/screen novel biofilm inhibitors (antimicrobial peptides/phytocompounds/synthetic compounds) and predict their biofilm-inhibition efficacy. Scrutinizing the contemporary <i>in silico</i> methods, a consolidated approach has been highlighted, referred to as a knowledge-guided computational pipeline for biofilm-targeted therapy. The proposed pipeline has amalgamated prominently employed methodologies in genomics, transcriptomics, interactomics and proteomics to identify potential target proteins and their complementary anti-biofilm compounds for effective functional inhibition of biofilm-linked pathways. This review can pave the way for new portals to formulate successful therapeutic interventions against biofilm-producing pathogens.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"928-947"},"PeriodicalIF":2.7,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138797202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efficacy of hypochlorous acid as an alternative oral antimicrobial agent on human gingival fibroblasts, <i>Aggregatibacter actinomycetemcomitans</i>, and <i>Candida albicans</i> biofilms <i>in vitro</i>.","authors":"Gözdem Bayraktar, Ayşe Mine Yılmaz Göler, Burak Aksu, Hafize Öztürk Özener","doi":"10.1080/08927014.2023.2288071","DOIUrl":"10.1080/08927014.2023.2288071","url":null,"abstract":"<p><p>This study compared the cytotoxicity and antimicrobial activity of hypochlorous acid (HOCl) at 50 ppm and 200 ppm and 0.2% chlorhexidine (CHX) at various time intervals, <i>in vitro</i>. Cell viability and cytotoxicity of human gingival fibroblasts (HGF) were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and the lactate dehydrogenase assay. Antimicrobial effects on <i>Aggregatibacter actinomycetemcomitans</i> and <i>Candida albicans</i> were determined using the time-kill method. All solutions exhibited a significant impact on HGFs in a dose- and time-dependent manner. 50 ppm HOCl demonstrated the highest cell viability, followed by 200 ppm HOCl. Both HOCl solutions were less cytotoxic to HGFs than 0.2% CHX. 50 ppm and 200 ppm HOCl demonstrated stronger efficiencies than CHX against <i>A. actinomycetemcomitans</i> and <i>C. albicans</i>. The data suggest that HOCl solutions have potential as an alternative antiseptic to CHX due to their lower cytotoxicity and superior antimicrobial activity, but optimal dosage of HOCl requires further investigations.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"980-989"},"PeriodicalIF":2.7,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138450788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}