Biofouling最新文献

{"title":"Repurposing GS-441524 to inhibit biofilm formation and virulence of <i>Bacillus cereus</i>.","authors":"Kai-Zhong Xu, Di Meng, Lu-Jun Yin, Min Wang, Jing Li","doi":"10.1080/08927014.2026.2730440","DOIUrl":"10.1080/08927014.2026.2730440","url":null,"abstract":"<p><p><i>Bacillus cereus</i> is a resilient foodborne pathogen, with biofilm formation and virulence regulated by quorum sensing (QS). This study evaluates GS-441524, the active metabolite of remdesivir, as an anti-virulence agent against B. cereus. At sub-inhibitory concentrations, GS-441524 dose-dependently reduced protease, lipase, hemolysin, and AI-2 production, impaired motility, and inhibited biofilm formation. Gene expression analysis showed downregulation of key QS (<i>papR, luxS, plcR</i>) and toxin (<i>hblD, cytK, nprB</i>) genes. Molecular docking indicated strong binding to HblD. In a <i>Galleria mellonella</i> model, GS-441524 enhanced survival upon <i>B. cereus</i> challenge. These findings demonstrate that GS-441524 disrupts QS and virulence, offering a promising strategy to control <i>B. cereus.</i></p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"962-973"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148928834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efflux pump inhibition disrupts biofilms and enhances azole susceptibility in <i>Candidozyma auris</i>.","authors":"Kevin Eduardo Castro, Maria Laina Silva, Francisco Eilton Sousa Lopes, Ana Raquel Colares de Andrade, Benaya Sanders Carneiro, Lua Silva, João Moreira de Matos Neto, José Júlio Costa Sidrim, D S C M Castelo-Branco, Gláucia Morgana de Melo Guedes, Bruna Rodrigues de Sousa, Camylla Carvalho de Melo, Reginaldo Gonçalves de Lima-Neto, Rossana de Aguiar Cordeiro","doi":"10.1080/08927014.2026.2734116","DOIUrl":"10.1080/08927014.2026.2734116","url":null,"abstract":"<p><p><i>Candidozyma auris</i> (formerly <i>Candida auris</i>) is a global emerging fungal pathogen of significant global clinical and epidemiological concern. It is frequently associated with hospital outbreaks, largely due to its multidrug resistance, ability to persist on medical devices, and high potential for nosocomial transmission. This study investigated efflux pump inhibition as a potential strategy for controlling <i>C. auris</i>. The efflux pump inhibitor promethazine (PMZ) was evaluated against planktonic cells and mature biofilms of 15 clinical isolates. PMZ completely inhibited the planktonic growth of both azole-susceptible and azole-resistant <i>C. auris</i> strains at concentrations ranging from 32 to 64 μg/mL. Synergistic interactions between PMZ and either echinocandins or azoles were observed, resulting in effective inhibition of nearly all tested strains during planktonic phase growth. Additionally, PMZ at 4× MIC significantly disrupted mature biofilms of both azole-susceptible and azole-resistant strains. Mature biofilms were also susceptible to combinations of antifungals and PMZ. These findings indicate that efflux pump inhibition represents a promising therapeutic strategy for controlling infections caused by <i>C. auris.</i> PMZ may be considered a promising structural framework for the development of novel antifungal agents.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1039-1051"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term evaluation of \"Cajun coating,\" a capsaicin-enhanced copper antifouling coating for reducing marine biofouling.","authors":"Anna Reimer, Kathryn A O'Shaughnessy, Edward Sullivan, Steven Vrolijk, Charles W Martin","doi":"10.1080/08927014.2026.2734112","DOIUrl":"10.1080/08927014.2026.2734112","url":null,"abstract":"<p><p>Marine biofouling imposes significant operational and ecological costs for the maritime industry, including increased hydrodynamic drag, material degradation, and the spread of non-native species. This study investigated the use of cayenne pepper to reduce fouling on submerged hard substrate - a method sometimes used by recreational boat owners for anti-fouling and referred to as \"Cajun coating.\" We ran a preliminary short-term efficacy study to evaluate whether capsaicin, the active ingredient in cayenne pepper, enhances the effectiveness of traditional copper-based antifouling coatings under <i>in-situ</i> conditions. Fiberglass-coated tiles were assigned six treatments (copper only, copper with 0.5%, 1%, or 2% capsaicin, copper plus methylated seed oil, and an uncoated tile control) and were deployed in the marine environment for six weeks in coastal Alabama. Nine taxa were recorded, with taxa richness highest on controls and lowest on 2% capsaicin tiles. Community composition differed significantly among treatments, with soft-bodied taxa (e.g. soft tube-dwelling polychaetes, hydroids) declining in response to capsaicin, whereas barnacles showed minimal response. Detritus and bare space generally increased with capsaicin concentration. Notably, bare space - perhaps the most relevant indicator of antifouling efficacy - was significantly greater in the 2% capsaicin treatment than in the copper treatments, indicating enhanced antifouling performance with incorporation of capsaicin. Largest differences were also seen between 1% and 2% capsaicin treatments, particularly in total mean taxa abundance and percent detritus. Overall, capsaicin enhanced antifouling performance in a concentration-dependent manner, particularly against soft-bodied taxa and increasing bare space, suggesting this approach has potential as an environmentally benign antifouling option. Our results provide preliminary <i>in-situ</i> research-based evidence for this low-technology and cost-effective approach to reduce marine biofouling on infrastructure and recreational vessels.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1027-1038"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolving frontiers in drinking water membrane technology: a 25-year bibliometric and antifouling perspective.","authors":"Zhen-Hua Tian, Ting Yang, Meng-Qi Tian, Peng-Fei Li","doi":"10.1080/08927014.2026.2718246","DOIUrl":"10.1080/08927014.2026.2718246","url":null,"abstract":"<p><p>Based on a comprehensive dataset of 5,925 publications retrieved from Scopus and the Web of Science Core Collection between 2000 and 2024, this study systematically maps the research landscape of membrane technology for drinking water production <i>via</i> integrated bibliometric analysis and visualization using VOSviewer, Origin 2023, and R statistical software. The results indicated that annual publications increased by approximately 1,361% (from 36 in 2000 to 526 in 2024), with China and USA collectively contributing about 52% of global publications. Core journals in this domain include <i>Water Research</i>, <i>Journal of Membrane Science</i>, and <i>Chemosphere</i>. Early pioneering work was carried out by Bart Van der Bruggen, while Liang Heng from Harbin Institute of Technology has become one of the most productive researchers in recent years. Notably, the research focus has gradually shifted from membrane processes to membrane materials. While previous reviews have largely focused on technical optimization without systematic quantification of research trends or collaboration networks, this study fills that gap by providing the first large‑scale bibliometric mapping of the field's evolution, geographic hotspots and emerging themes. Additionally, this study reviews the research progress in antifouling strategies, encompassing membrane cleaning techniques and membrane surface modification, aiming to provide valuable insights for researchers worldwide.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"903-919"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148824646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofilm-producing <i>Acinetobacter baumannii</i> in Asia: mapping the clinical burden and antimicrobial resistance through systematic review and meta-analysis.","authors":"Indu Singh, Anil Kumar, Indira Kumari Verma, Divakar Sharma, Megh Singh Dhakad, Mirza Masroor Ali Beg","doi":"10.1080/08927014.2026.2732046","DOIUrl":"10.1080/08927014.2026.2732046","url":null,"abstract":"<p><p><i>Acinetobacter baumannii</i>, an evolving pathogen that has become a drug-resistant strain in the last two decades, causes a high rate of infections in health-care settings. Most nosocomial infections were caused by biofilm-forming <i>A. baumannii,</i> which enables it to survive and proliferate under hostile conditions, including high concentrations of antibiotics. They confer resistance to antibiotics and facilitate the spread of drug-resistance genes among strains. For alternative preventive measures, patterns of <i>A. baumannii</i> are required, which are still in their infancy, especially in Asia. Thus, the current systematic review and meta-analysis were conducted to evaluate the Asian prevalence of biofilm-forming <i>A. baumannii</i> in clinical isolates. Data extraction was performed by searching various electronic databases and was collected in Microsoft Excel. The extracted data were exported to STATA version 12 for further statistical analysis. The pooled prevalence of biofilm-forming <i>A. baumannii</i> was evaluated using random effects with DerSimonian-Laird. Sensitivity analysis was performed to assess the impact of individual reports on the pooled prevalence. A funnel plot was used to assess publication bias, and in addition to confirm, Egger's statistical test was employed. The prevalence of biofilm-forming <i>A. baumannii</i> in Asia was 67% (95% CI = 58.50, 75.31). A significant heterogeneity was observed among studies with an <i>I</i>² of 98.8%. The prevalence of strong, moderate and weak biofilm-forming <i>A. baumannii</i> was 20.37% (95% CI = 19.31, 21.43), 30.28% (95% CI = 29.17, 31.40) and 0%. The highest prevalence of biofilm-forming <i>A. baumannii</i> clinical isolates was reported for China (99.04%; 95% CI = 97.2, 100.9). The prevalent gene linked to biofilm-forming <i>A. baumannii</i> clinical isolates was the <i>ompA</i> gene (65.38%). The increased biofilm-forming burden of <i>A. baumannii</i> in healthcare settings indicates the need for routine patient screening and the prevention of emerging multidrug resistant <i>A. baumannii</i>. Moreover, these outcomes suggested the design of a novel prevention program by public-health policymakers to control the looming drug-resistant <i>A. baumannii</i> strain.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"974-986"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148981938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-settlement activity of marine-inspired micropeptides against model soft and hard macrofoulers <i>Ciona savignyi</i> and <i>Amphibalanus improvisus</i>.","authors":"Srinivas Venkatnarayanan, Swantje Enge, Alexander J Craig, Lindon W K Moodie, Yuri Ermolovich, Henrik Pavia, Natalia Molchanova, Patrick L Cahill, Johan Svenson","doi":"10.1080/08927014.2026.2725685","DOIUrl":"10.1080/08927014.2026.2725685","url":null,"abstract":"<p><p>The current study describes the evaluation of 16 synthetic linear tripeptides combining structural elements from the innate defence system with brominated amino acids often found in antifouling compounds isolated from sessile marine organisms. These organisms have clean exteriors and their chemical arsenal of natural antifoulants is interesting from an antifoulant design perspective. The peptides were evaluated for their ability to inhibit the settlement and metamorphosis of the soft and hard macrofoulers <i>Ciona savignyi</i> and <i>Amphibalanus improvisus</i> which are two key macrofoulers challenging to remove once established. It was shown that the evaluated compounds were generally highly active antifoulants with low micromolar IC₅₀-values against both fouling organisms <i>via</i> different mechanisms. Several of the compounds were shown to be biocidal against <i>Ciona savignyi</i> larvae at the lowest evaluated concentration (1 µM). Against <i>Amphibalanus improvisus</i>, a non-biocidal antifouling effect was generally observed with the most potent compound displaying an IC₅₀-value of 0.5 µM. The study illustrated how these two groups of natural compounds combined are highly bioactive and can yield highly efficient antifoulants.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"929-941"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic insights into microbial biodeterioration of terracotta in the 18th-century-old temple Jor Mandir.","authors":"Shouvik Pramanik, Sayan Banerjee, Arka Mondal, Anupam Kundu, Raju Biswas, Bomba Dam, Sikha Mandal, Jnanendra Rath","doi":"10.1080/08927014.2026.2734111","DOIUrl":"10.1080/08927014.2026.2734111","url":null,"abstract":"<p><p>Microbial colonisation represents a significant yet underexplored factor contributing to the biodeterioration of terracotta heritage structures in tropical environments. Here, we present a comprehensive multi-analytical investigation of the Jor Mandir complex, an eighteenth-century terracotta monument in eastern India. Culture-based analysis revealed morphologically distinct cyanobacteria that were not detected by metagenomics, underscoring the complementarity of the two approaches. Amplicon sequencing of a composite biofilm sample revealed a microbial community dominated by Cyanobacteria (44.18%), followed by Actinobacteriota (18.61%), Proteobacteria (14.30%), Chloroflexi (10.16%), Acidobacteriota (7.38%), and other minor phyla. Stress-tolerant coccoid taxa such as <i>Chroococcidiopsis</i> and <i>Aphanocapsa</i>, along with filamentous genera including <i>Leptolyngbya</i>, <i>Lyngbya</i>, <i>Oscillatoria</i>, and <i>Phormidium</i>, and heterocystous nitrogen-fixing genera such as <i>Scytonema</i>, <i>Nostoc</i>, and <i>Calothrix</i>, were identified. These organisms exhibited biochemical adaptations, including the production of scytonemin and carotenoids, which contributed to photoprotection and substrate weathering. Elemental profiling indicated associations between the aluminosilicate terracotta matrix and surface-associated biofilm material. GC-MS analysis revealed a range of organic compounds, including fatty acid methyl esters, hydrocarbons, phenolic compounds, and phthalate derivatives, reflecting the chemically complex nature of the biofilm matrix. PICRUSt2-based functional reconstruction further indicated a biofilm community with predicted pathways related to biosynthetic processes, biodegradation pathways, and energy metabolism. Together, these descriptive observations provide a site-specific baseline profile of microbial colonisation and biofilm-associated characteristics on the terracotta surfaces of the Jor Mandir complex and highlight the value of integrating microbiological and physicochemical approaches in future monument monitoring and conservation studies.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"987-1002"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanobiology-aware autonomous antifouling interfaces: multimodal sensing, adaptive actuation, and closed-loop biofouling control.","authors":"Muhammad Saeed Akhtar, Wajid Zaman","doi":"10.1080/08927014.2026.2739961","DOIUrl":"https://doi.org/10.1080/08927014.2026.2739961","url":null,"abstract":"<p><p>Biofouling is a dynamic, spatially heterogeneous, and history-dependent interfacial process, whereas most control strategies remain static or calendar-based. This mismatch can cause unnecessary chemical and energy use, delayed intervention, coating damage, and incomplete recovery. This critical review develops a mechanobiology-aware framework for autonomous antifouling interfaces that detect a fouling-relevant signal, estimate latent interfacial state, select a bounded action, actuate locally, and verify recovery. It links conditioning-film ageing, bacterial surface sensing, cyclic di-GMP-mediated commitment, extracellular polymeric substance assembly, collective mechanics, transport limitation, community interactions, and dispersal to optical, electrochemical, acoustic, mechanical, and molecular sensing. Electrochemical, optical, thermal, acoustic, hydrodynamic, topographic, hydrated or liquid-infused, enzymatic, and quorum-interference actuation are evaluated in relation to target state and failure mode. Passive resistance, pre-programmed responsiveness, and genuine closed-loop operation are distinguished explicitly. The proposed architecture treats the fouled interface as a partially observed system in which multimodal measurements support an uncertainty-aware state estimate, a constrained policy selects the least harmful action likely to succeed, and post-action sensing verifies biological and functional recovery. Reduced digital-twin models are positioned as decision-support tools rather than as established universal solutions. Key deployment requirements include calibration transfer, sensor self-fouling, latency, material ageing, detached-biomass capture, release accounting, safe fallback, and direct comparison with the identical actuator operated open-loop.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-22"},"PeriodicalIF":2.5,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-destructive monitoring of antibiotic action in <i>Bacillus subtilis</i> biofilms using dual-parameter swept-source optical coherence tomography.","authors":"Nafisa Sultana, Raju Poddar","doi":"10.1080/08927014.2026.2736790","DOIUrl":"https://doi.org/10.1080/08927014.2026.2736790","url":null,"abstract":"<p><p>Biofilms show an enhanced tolerance to antimicrobials compared to planktonic cells and conventional susceptibility tests provide limited insight into antibiotic action within intact biofilms. We assessed swept-source optical coherence tomography (SSOCT) as a non-destructive, label-free platform for biofilm monitoring, extracting two label-free parameters, the optical attenuation coefficient (μ_t) and biospeckle temporal contrast (γ). Biofilms cultured for 24 h, were treated with antibiotics at minimum biofilm eradication concentration (MBEC) determined by the Calgary biofilm device and imaged at 0, 24 and 48 h post-treatment alongside time-matched untreated controls, with parallel colony forming unit (CFU) enumeration. Untreated biofilms showed progressive increases in both μ_t and γ, confirming that time-matched rather than pre-treatment controls are required. Treated biofilms showed elevated μ_t (76.9-103.2% above control, <i>p</i> < 0.001) but declining γ (68.4% and 68.6% below control at 48 h, <i>p</i> < 0.001), with the μ_t-γ correlation reversing from positive during maturation (r = +0.93) to negative under treatment (r = -0.94). Viable counts decrease by ∼2.5 log₁₀ CFU/mL in both treated arms (p < 0.001), confirming substantial killing; γ nonetheless declined nonlinearly with respect to viability and continued falling after CFU/mL stabilized, indicating that γ reflects progressive physical quiescence rather than viable cell numbers. SSOCT attenuation and biospeckle thus offer a non-destructive complement to conventional viability assays.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-16"},"PeriodicalIF":2.5,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149029626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass transfer: the invisible architecture of biofilms.","authors":"Manuel Simões","doi":"10.1080/08927014.2026.2718245","DOIUrl":"10.1080/08927014.2026.2718245","url":null,"abstract":"","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"783-785"},"PeriodicalIF":2.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}