Biofouling最新文献

{"title":"Crevice corrosion of CuNi 90/10 under simulated macrofouling in temperate and tropical Australian marine environments.","authors":"M A Javed, R Piola, W C Neil, S A Wade","doi":"10.1080/08927014.2026.2630944","DOIUrl":"10.1080/08927014.2026.2630944","url":null,"abstract":"<p><p>Copper-nickel 90/10 alloys are widely used in marine environments due to their resistance to corrosion and biofouling. However, the influence of macrofouling on localised corrosion remains poorly understood. This study examines the effect of simulated macrofouling-induced crevices on CuNi 90/10 corrosion using laser-cut plastic shims to replicate hard fouling geometries. Samples with crevice gaps of 50, 130 and 1,000 µm were exposed for 180 days at two Australian marine sites: temperate (Melbourne) and tropical (Cairns). Crevice corrosion was most severe in Melbourne, with the 130 µm gap showing the deepest attack. In Cairns, samples showed lower crevice corrosion despite heavier external fouling, suggesting that fouling composition and protective calcareous deposits may mitigate attack. Crevice interiors lacked Desulfobacterota and exhibited less diverse communities with greater inter-replicate variability at both sites than outside surfaces. These results highlight the combined influence of environment, crevice geometry, corrosion product films, and biofouling composition on crevice corrosion of CuNi 90/10.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"391-410"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147301667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myricetin as a potential therapeutic agent against <i>Pseudomonas aeruginosa</i>: inhibition of biofilm formation, quorum sensing, and virulence factor production.","authors":"Sallah A Al Hashedi, Saif M Dmour, Hamad Sarheed Galut, Ayman Daifallah Ismail Alsheikh, Mohammad H Abukhalil, Sham Ma'aitah, Najeeb S Al-Zoreky, Khaled M A Ramadan, Muhammad Naeem Sattar, Zafar Iqbal, Adil Alshoaibi, Sultan A M Saghir","doi":"10.1080/08927014.2026.2634395","DOIUrl":"10.1080/08927014.2026.2634395","url":null,"abstract":"<p><p>Multidrug-resistant pathogenic bacteria, particularly <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>), pose a significant threat to human health. Despite the huge persistence of antibiotics, there remains a lack of effective natural compounds capable of simultaneously disrupting quorum sensing (QS), biofilm formation, and virulence in this pathogen. This study aimed to investigate the inhibitory potential of myricetin against <i>P. aeruginosa</i> focusing on its ability to interfere with QS-regulated virulence traits. Antibacterial, antibiofilm, anti-QS, and virulence factor activities were evaluated using crystal violet biofilm formation and QS-regulated virulence factor inhibition assays (e.g. pyocyanin, rhamnolipid, protease, and exopolysaccharides). The minimum inhibitory concentration (MIC) required to inhibit visible bacterial growth was 0.97 mg/mL. Additionally, the minimum biofilm inhibitory concentration of 50 (MBIC₅₀) was recorded at the MIC value. Myricetin showed a significant inhibitory effect against biofilm formation by suppressing bacterial hydrophobicity, aggregation, and swarming motility. Furthermore, myricetin significantly reduced the production of pyocyanin, rhamnolipid, protease, and exopolysaccharides. The myricetin effectively impeded QS mechanisms as evidenced by a significant reduction in the production of acyl homoserine lactone and violacein pigment, both qualitatively and quantitatively. Gene expression analysis exhibited a significant downregulation of <i>LasI</i>/<i>R</i> and <i>RhlI</i>/<i>R</i> genes, further enhancing the myricetin role in QS inhibition. Collectively, these findings demonstrate that myricetin effectively interferes with QS-mediated virulence mechanisms in <i>P. aeruginosa</i>, supporting its potential as a promising lead compound for developing anti-virulence strategies.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"429-445"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the impact of dust deposition and CO₂ level on biofilm development causing biodeterioration of historic buildings and monuments in India.","authors":"Riya Singh, Ilma Khan, Riya Sharma, Saurabh Mishra, Lalit Kumar Pandey","doi":"10.1080/08927014.2026.2645567","DOIUrl":"https://doi.org/10.1080/08927014.2026.2645567","url":null,"abstract":"<p><p>The deterioration of buildings and historic monuments due to phototrophic biofilms, dust, and carbon dioxide concentration is an increasingly significant global issue, but remains insufficiently studied. In this research, phototrophic biofilms were collected from nine sampling sites, which were categorized as control, moderately contaminated, and severely contaminated based on the air quality index and canopy cover. Severely contaminated sites showed higher temperatures, CO₂ levels, and dust accumulation, but lower humidity. Cyanobacteria dominated all biofilm samples, followed by fungal hyphae and spores. Green algae and diatoms contributed minimally. Healthy diatom frustules decreased sharply with increasing contamination, from the control sites to severely contaminated sites. Regression analysis revealed significant positive correlations between cyanobacterial abundance and both CO₂ levels and dust deposition, indicating that increased air pollutants promote the growth of cyanobacteria and diatoms in phototrophic biofilms, further highlighting their potential as a sensitive bioindicator of environmental contamination affecting cultural heritage sites.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-15"},"PeriodicalIF":2.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of β-lactam resistance in dual-species biofilms of <i>Pseudomonas aeruginosa</i> and methicillin-sensitive <i>Staphylococcus aureus</i>.","authors":"Waleska S da Cruz Nizer, Kira N Allison, Carole Beaulieu, Megan C Montgomery, Edana Cassol, Joerg Overhage","doi":"10.1080/08927014.2026.2620072","DOIUrl":"10.1080/08927014.2026.2620072","url":null,"abstract":"<p><p>Polymicrobial biofilms are frequently associated with chronic infections and are highly tolerant to antibiotic treatment. Given that β-lactam antibiotics are the most prescribed antibiotics worldwide, we investigated β-lactam resistance in dual-species biofilms formed by <i>Pseudomonas aeruginosa</i> PAO1 and methicillin-sensitive <i>Staphylococcus aureus</i> (MSSA). Compared to monocultures, MSSA exhibited reduced susceptibility to cephalexin, ampicillin, and cefazolin, while <i>P. aeruginosa</i> showed reduced susceptibility to cefazolin and nafcillin within dual-species biofilms. Using a set of <i>P. aeruginosa</i> mutants, we demonstrate that the PAO1 AmpC β-lactamase, the exopolysaccharides Psl and Pel, and the quorum sensing regulators LasR and RhlR each play significant roles in protecting MSSA from β-lactam treatment. Interestingly, co-cultures of MSSA with the Δ<i>pslA pelF</i> mutant strain increased the survival of Δ<i>pslA pelF</i> under β-lactam exposure. Overall, these findings advance our understanding of how interdependent bacterial interactions compensate for the loss of matrix components and thereby contribute to antimicrobial tolerance in polymicrobial biofilms.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"278-293"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146059325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of location and coating on the development of an in-water grooming program to maintain ship hull coatings.","authors":"Melissa Tribou, Geoffrey Swain, Harrison Gardner, Sam Royster, Jarema M Didoszak, Dean Webster, Joseph Dahlgren","doi":"10.1080/08927014.2026.2621192","DOIUrl":"10.1080/08927014.2026.2621192","url":null,"abstract":"<p><p>Ship hull grooming, a form of proactive in-water cleaning, has been extensively researched in a warm, productive, subtropical Atlantic location using US Navy-approved commercial coatings. Less is known about how grooming performs on toughened fouling control coatings or the grooming frequencies required in locations with lower seawater temperatures and lower fouling intensities. The purpose of this study was to explore differences in proactive in-water cleaning schedules due to changes in location and coating types. This study compared the time-based frequency of in-water grooming of two commercial and two experimental fouling control coatings in Port Canaveral, Florida, and Monterey, California. The results demonstrated that a higher grooming frequency was required to maintain surfaces free of fouling at the warm-water Port Canaveral, FL site compared to the cooler-water site in Monterey, CA. They also showed that the type of fouling control coating will influence the grooming frequency.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"305-323"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of capsaicin in antifouling technologies in marine environments.","authors":"Huanchao Shen, Mulan Mu, Zhiwei Jiao","doi":"10.1080/08927014.2026.2628690","DOIUrl":"10.1080/08927014.2026.2628690","url":null,"abstract":"<p><p>Marine biofouling has been negatively affecting human sailing since ancient times. Traditional antifouling coatings, such as tributyl tin-based coatings, were banned globally in 2008 due to their inherent toxicity and severe threat to the marine environment. Developing environmentally friendly, long-term marine antifouling coatings remains a huge challenge for the maritime industry. Capsaicin is one of the natural product antifoulants (NPAs). Capsaicin is favored by researchers due to its good antifouling effect and low toxicity. This review provides insights into four methods of application of capsaicin in the field of antifouling: incorporation into coatings, encapsulation <i>via</i> microcapsules, synthesis and exploration of capsaicin derivatives and incorporation into membranes to enhance the antifouling properties of materials. This paper compares the experimental data of various applied methods in the hope of enlightening interested researchers.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"233-249"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146211931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/08927014.2026.2626152","DOIUrl":"10.1080/08927014.2026.2626152","url":null,"abstract":"","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"338"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro performance of eugenol and cinnamaldehyde on biofilms produced by <i>Candidozyma auris</i>.","authors":"Débora Silva Marques de Sousa, Gabrielle Pires de Morais Monari, Cassiana Ferreira da Rosa, Ana Flávia Marques Pereira, Tatiane Baptista Zapata, Cauê Bastos Tertuliano Dos Santos, Ary Fernandes Júnior, Sandra de Moraes Gimenes Bosco, Marcia de Souza Carvalho Melhem, Luciana da Silva Ruiz","doi":"10.1080/08927014.2026.2613950","DOIUrl":"10.1080/08927014.2026.2613950","url":null,"abstract":"<p><p><i>Candidozyma auris</i> has high rates of resistance to antifungals and hospital disinfectants. This yeast remains on surfaces for long periods, favoring the colonization of patients. Similarly <i>Candida</i> species, it produces persistent biofilms, which contribute to invasive infections, resulting in high mortality rates. In the search for new therapeutic alternatives of antifungal action, natural products have been widely studied due to their pharmacological potential and diverse biological activities. Eugenol and cinnamaldehyde are derived from the essential oils of cinnamon and clove, respectively, and have shown action against several microorganisms, including <i>Candida</i> species. The aims of the present study were to evaluate the antibiofilm activity of the natural products eugenol and cinnamaldehyde on clinical isolates of <i>C. auris</i>. Both products showed inhibitory action on planktonic form. Biofilm biomass was considerably reduced for most of the evaluated isolates. On the other hand, during the treatment period, the metabolic activity of most isolates increased, compared to control, possibly due to the heteroresistance phenomenon; however, further studies are still needed to support this hypothesis.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"250-263"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevention of <i>Staphylococcus aureus</i> biofilm formation on catheters, contact lenses, and contact lens cases by a synthetic analogue of the antimicrobial lipopeptide humimycin.","authors":"Md Ramim Tanver Rahman, Ismail Fliss, Eric Biron","doi":"10.1080/08927014.2026.2621190","DOIUrl":"10.1080/08927014.2026.2621190","url":null,"abstract":"<p><p>Biofilm-associated infections, particularly those caused by <i>Staphylococcus aureus</i>, pose a persistent challenge in clinical settings and in medical devices such as catheters, wound dressings, orthopaedic and dental implants, and contact lenses. The resilience of biofilms to conventional antimicrobial treatments underscores the need for innovative strategies. This study evaluated the efficacy of a synthetic analogue of the antimicrobial lipopeptide humimycin as a preventive measure against biofilm formation of <i>S. aureus</i> on catheters, contact lenses and contact lens cases, both as a coating agent and in combination with commercial multipurpose contact lens solutions. The results demonstrate a dose-dependent reduction in biofilm formation with the humimycin analogue coatings achieving up to 98% inhibition at 256 µg/mL. This significant reduction was accompanied by a marked decrease in bacterial metabolic activity, indicating that the humimycin analogue disrupts biofilm integrity and impairs bacterial function. When combined with commercial contact lens solutions such as Opti-Free^® Express^® and Solocare Aqua^®, the biofilm-disrupting activity of the lipopeptide was further enhanced, with lower minimum inhibitory concentration and minimum biofilm inhibitory concentration values. These findings suggest that the humimycin analogue holds significant promise as a novel antimicrobial strategy for preventing <i>S. aureus</i> biofilm-related infections acquired from medical devices.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"294-304"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The action of selected monoterpenes as biofilm control agents and antibiotic resistance modifiers.","authors":"Francisca Massano, Ana C Afonso, Mariana Sousa, Lília S Teixeira, Anabela Borges, Manuel Simões","doi":"10.1080/08927014.2026.2615081","DOIUrl":"10.1080/08927014.2026.2615081","url":null,"abstract":"<p><p>Multidrug-resistant bacteria in biofilms are a growing public health threat, due to their resistance to conventional antibiotics. Phytochemicals are attractive candidates because of their structural diversity and ability to potentiate antimicrobial activity. This study investigated the antibiofilm and resistance-modifying effects of two monoterpenes, menthol and linalool, alone and in combination with ten antibiotics, against <i>Escherichia coli</i> and <i>Staphylococcus epidermidis</i>. Menthol exhibited MIC and MBC of 800 µg/mL against <i>E. coli</i> and the same MIC against <i>S. epidermidis</i>, while linalool showed MICs of 800 µg/mL and 400 µg/mL, respectively. Combination assays revealed enhanced activity of erythromycin with both monoterpenes against <i>E. coli</i> and of amoxicillin with menthol against <i>S. epidermidis</i>, although sessile cells were largely unaffected. When applied individually, both monoterpenes caused a 3-log reduction in culturable <i>E. coli</i> biofilm cells. The overall findings highlight the antibiofilm activity of linalool and, particularly, menthol, supporting their role as antibiotic adjuvants against biofilm-associated infections.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"264-277"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}