Biofouling最新文献_第2页

NMR-based metabolomics of Burkholderia pseudomallei biofilms and extracellular polymeric substance cultured in LB and MVBM media. 假马利氏伯克氏菌生物膜和细胞外聚合物在LB和MVBM培养基中培养的核磁共振代谢组学。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-05-01 Epub Date: 2025-05-14 DOI: 10.1080/08927014.2025.2502936

Suthantip Srithabut, Pisit Chareonsudjai, Thotsapol Chaianunporn, Chainarong Bunma, Jutarop Phetcharaburanin, Manida Suksawat, Suwalak Chitcharoen, Sorujsiri Chareonsudjai

{"title":"NMR-based metabolomics of Burkholderia pseudomallei biofilms and extracellular polymeric substance cultured in LB and MVBM media.","authors":"Suthantip Srithabut, Pisit Chareonsudjai, Thotsapol Chaianunporn, Chainarong Bunma, Jutarop Phetcharaburanin, Manida Suksawat, Suwalak Chitcharoen, Sorujsiri Chareonsudjai","doi":"10.1080/08927014.2025.2502936","DOIUrl":"10.1080/08927014.2025.2502936","url":null,"abstract":"Burkholderia pseudomallei biofilms are resistant to antibiotics and immune responses, leading to persistent infections. This study aimed to investigate the metabolic profiles of B. pseudomallei in biofilms and the extracellular polymeric substances (EPS) produced during grown in LB or MVBM medium using Nuclear Magnetic Resonance (NMR) spectroscopy to identify key metabolites. The results revealed similar biofilm metabolites in both media. However, betaine was detected in LB, but not in the case of MVBM. Acetate was significantly higher in MVBM compared to that of LB. Pathway analysis revealed that betaine-producing B. pseudomallei biofilm in LB was associated with metabolism of glycine, serine, and threonine, while acetate in MVBM was associated with metabolism of taurine and hypotaurine, phosphonate and phosphinate, and glycolysis/gluconeogenesis. The NMR analysis of EPS disclosed shared metabolites including dimethylsulfide, 1-methyluric acid and oxypurinol. This study provides the first extensive investigation into B. pseudomallei biofilm and EPS metabolites, identifying pathways that offer potential targets for combating B. pseudomallei biofilm-associated infections.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"498-511"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sweeteners affect biofilm formation and virulence gene expression in Pseudomonas aeruginosa PAO1. 甜味剂影响铜绿假单胞菌PAO1的生物膜形成和毒力基因表达。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-05-01 Epub Date: 2025-05-19 DOI: 10.1080/08927014.2025.2504023

Tamara Carević Milićević, Katarina Novović, Biljana Nikolić, Dejan Stojković, Vuk Maksimović, Dragica Milosavljević, Marija Ivanov

{"title":"Sweeteners affect biofilm formation and virulence gene expression in Pseudomonas aeruginosa PAO1.","authors":"Tamara Carević Milićević, Katarina Novović, Biljana Nikolić, Dejan Stojković, Vuk Maksimović, Dragica Milosavljević, Marija Ivanov","doi":"10.1080/08927014.2025.2504023","DOIUrl":"10.1080/08927014.2025.2504023","url":null,"abstract":"Pseudomonas aeruginosa is an opportunistic pathogen able to form biofilms, contributing to its virulence. With the increasing use of sweeteners in various foods, understanding their influence on bacterial behavior is critical. This study investigated the virulence of P. aeruginosa PAO1 exposed to sweeteners (erythritol, stevia, fructose, coconut sugar, cane sugar, demerara). Sweeteners didn't affect growth rates. Erythritol stimulated biofilm (100 µg/mL, 159.98% formation), while 10 µg/mL of coconut sugar, cane sugar, and demerara promoted lower levels (∼70% formation). Erythritol stimulated exopolysaccharides production but reduced biofilm eDNA. Stevia, fructose, and coconut sugar increased the expression of lasI, lasR, rhlI, rhlR, pqsA, mvfR, and pvdF. HPLC analysis confirmed sucrose as the major sugar in demerara, coconut and cane sugar. Erythritol stimulated biofilm and some virulence genes expression, while other sweeteners' effects varied. Cane sugar was a biofilm inhibitor with a limited gene expression effect. The sweeteners' impact on microorganisms is diverse and should be further investigated.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"512-522"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold nanorods non-functionalised and associated with gallic acid exhibit activity against non-albicans Candida species. 与没食子酸相关的非功能化金纳米棒具有抗非白色念珠菌的活性。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-05-01 Epub Date: 2025-05-19 DOI: 10.1080/08927014.2025.2504026

Paulo Henrique Fonseca Carmo, Maria Fernanda Siqueira Fernandes da Costa, Anna Carolina Pinheiro Lage, Maíra Terra Garcia, Juliana Campos Junqueira

{"title":"Gold nanorods non-functionalised and associated with gallic acid exhibit activity against non-albicans Candida species.","authors":"Paulo Henrique Fonseca Carmo, Maria Fernanda Siqueira Fernandes da Costa, Anna Carolina Pinheiro Lage, Maíra Terra Garcia, Juliana Campos Junqueira","doi":"10.1080/08927014.2025.2504026","DOIUrl":"10.1080/08927014.2025.2504026","url":null,"abstract":"Strategies focusing on natural compounds and nanotechnology have been explored to overcome the limitations of conventional therapies in managing Candida infections. In this context, metal nanoparticles, both non-functionalised and combined with gallic acid, may offer a promising alternative. This study investigated the effects of gold nanoparticles non-functionalised (AuNp) and associated with gallic acid (AuNpGA) against planktonic cells and biofilms of Nakaseomyces glabratus, Pichia kudriavzevii, Candida parapsilosis, and Candida tropicalis. Both AuNp and AuNpGA inhibited the growth of all strains at 1.56 µg/mL and exhibited fungicidal effects at concentrations ranging from 1.56 to 3.12 µg/mL. The time-kill curve revealed that AuNpGA and AuNp completely inhibited the viability of all strains in planktonic cultures at 8 and 24 h, respectively, exhibiting greater antifungal activity compared to fluconazole. Treatment with AuNp increased ROS production against N. glabratus and P. kudriavzevii. Oxidative stress was enhanced against all strains after treatment with AuNpGA, and exposure to this compound reduced ergosterol levels of P. kudriavzevii and C. parapsilosis. Furthermore, AuNpGA and AuNp significantly decreased the viability of all Candida biofilms at 7.8 and 15.6 µg/mL, respectively. In summary, both gold nanoparticles exhibited activity against planktonic cells and biofilms, suggesting their potential as agents for treating Candida infections.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"523-535"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dynamic microcosm biofilm model for root carious-like lesion development: analysis of demineralization and microbiological characterization. 根状龋样病变发展的动态微观生物膜模型：脱矿和微生物特性分析。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-05-01 Epub Date: 2025-05-22 DOI: 10.1080/08927014.2025.2507893

Tamires Timm Maske, Glenda Ávila Marques, Bruna Dalongaro Fritsch, Bruna Moraes Kremer, Maximiliano Sérgio Cenci, Pabulo Henrique Rampelotto, Rodrigo Alex Arthur

{"title":"A dynamic microcosm biofilm model for root carious-like lesion development: analysis of demineralization and microbiological characterization.","authors":"Tamires Timm Maske, Glenda Ávila Marques, Bruna Dalongaro Fritsch, Bruna Moraes Kremer, Maximiliano Sérgio Cenci, Pabulo Henrique Rampelotto, Rodrigo Alex Arthur","doi":"10.1080/08927014.2025.2507893","DOIUrl":"10.1080/08927014.2025.2507893","url":null,"abstract":"This study investigated the multifunctional oral cavity simulator (MOCS) in terms of microbial composition, functional profile, and dentin root demineralization. Microcosm biofilms were grown on dentin using human saliva for 4, 7, and 14 days, with exposure to sucrose and a mucin-enriched medium. Biofilms were analyzed for microbial viability and composition through CFU count and 16S-rRNA gene sequencing. Demineralization was quantified by percentage surface hardness change (%SHC), mineral loss (ML), and lesion depth (LD). The results showed microbial viability at all time points. After 7 days, aciduric/acidogenic and proteolytic organisms increased in abundance. The functional profile reflected the oscillations in microbial composition. No significant differences in %SHC, ML, or LD were observed across the time points. Carious lesions exhibited 60-70% SHC and 125-200 µm depth. MOCS was able to induce root carious lesions as result of microcosm biofilm metabolic activity, indicating its potential use in preclinical studies on root dentin caries.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"536-550"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining optimum conditions for biofilm formation by Salmonella isolates and anti-biofilm activity of cinnamon essential oil nanoemulsion. 确定分离沙门氏菌形成生物膜的最佳条件及肉桂精油纳米乳的抗生物膜活性。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-05-01 Epub Date: 2025-05-07 DOI: 10.1080/08927014.2025.2499714

Fatemeh Dadkhah, Javad Aliakbarlu, Hossein Tajik

引用次数: 0

The mechanistic pathways of extracellular polymeric substances in the inhibition of carbon steel corrosion. 胞外聚合物抑制碳钢腐蚀的机理途径。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-04-01 Epub Date: 2025-04-07 DOI: 10.1080/08927014.2025.2483739

Ping Xu, Xinyue Chen, Weijin Xi

引用次数: 0

Development of marine antifouling performance in hard fouling-release coatings. 船用硬脱污涂料防污性能研究进展。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-04-01 Epub Date: 2025-05-06 DOI: 10.1080/08927014.2025.2498027

Allanique T Hunter, Aaron J Cogger, Kristyn Boutilier, Kylie H Curnew, Katherine Purvis, Alexis Trevors, Russell C Wyeth

{"title":"Development of marine antifouling performance in hard fouling-release coatings.","authors":"Allanique T Hunter, Aaron J Cogger, Kristyn Boutilier, Kylie H Curnew, Katherine Purvis, Alexis Trevors, Russell C Wyeth","doi":"10.1080/08927014.2025.2498027","DOIUrl":"https://doi.org/10.1080/08927014.2025.2498027","url":null,"abstract":"Marine biofouling is a substantial economic and environmental issue. Hard fouling-release coatings present a promising solution, combining fouling-release characteristics with durability. This study tested proprietary hard fouling-release prototype coatings from GIT Coatings, Inc. alongside uncoated controls, colour controls, and commercial performance standards. Three successive experiments were completed, incorporating static and dynamic flow conditions at sites in Nova Scotia, Canada. Initially, biofouling percent cover and cleanability for prototype coatings were comparable to untreated controls. By the final experiment, prototype coatings had significantly lower percent covers than both uncoated controls and the durability performance comparison, Ecospeed. Furthermore, several prototype hard fouling-release coatings had comparable percent cover (and possibly cleanability) to the fouling-release performance comparison, Intersleek. The results indicate that hard fouling-release coatings with potentially greater durability and longevity can achieve similar fouling-release performance as commercial fouling-release coatings. Further tests are needed to determine if unintended toxicity contributes to the antifouling effects.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":"41 4","pages":"429-442"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring quinazoline-derived copper(I) complex coated intravaginal ring against vulvovaginal candidiasis causing Candida species. 探讨喹唑啉衍生铜(I)络合物阴道内环对引起假丝酵母菌的外阴阴道念珠菌病的防治作用。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-04-01 Epub Date: 2025-04-23 DOI: 10.1080/08927014.2025.2489479

Dhesiga Krishnan, Sudaarsan Aruna Senthil Kumar, Sowndarya Jothipandiyan, Venkatesan Yamuna Devi, Devarajan Suresh, Paramasivam Nithyanand

引用次数: 0

Effect of promethazine against Staphylococcus aureus and its preventive action in the formation of biofilms on silicone catheters. 异丙嗪对金黄色葡萄球菌的影响及其在硅胶导管上形成生物膜的预防作用。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-04-01 Epub Date: 2025-04-04 DOI: 10.1080/08927014.2025.2486250

Érica Rayanne Mota da Costa, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Thais Lima Ferreira, Maria Janielly Castelo Branco Silveira, Leilson Carvalho de Oliveira, Lívia Gurgel do Amaral Valente Sá, Cecília Rocha da Silva, João Batista de Andrade Neto, Sorele Facundo da Silva, Bruno Coêlho Cavalcanti, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior

{"title":"Effect of promethazine against Staphylococcus aureus and its preventive action in the formation of biofilms on silicone catheters.","authors":"Érica Rayanne Mota da Costa, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Thais Lima Ferreira, Maria Janielly Castelo Branco Silveira, Leilson Carvalho de Oliveira, Lívia Gurgel do Amaral Valente Sá, Cecília Rocha da Silva, João Batista de Andrade Neto, Sorele Facundo da Silva, Bruno Coêlho Cavalcanti, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior","doi":"10.1080/08927014.2025.2486250","DOIUrl":"10.1080/08927014.2025.2486250","url":null,"abstract":"Urinary infections caused by Staphylococcus aureus are commonly associated with urinary catheterization and often result in severe complications. Given this problem, the objective of the study was to investigate the preventive action of promethazine (PMT) against the formation of methicillin-resistant Staphylococcus aureus (MRSA) biofilms when impregnated in urinary catheters. For this purpose, techniques such as broth microdilution, checkerboard, impregnation on urinary catheter fragments, flow cytometry assays and scanning electron microscopy were employed. PMT exhibited antimicrobial activity with Minimum Inhibitory Concentration (MIC) values ranging from 171 to 256 µg/mL, predominantly additive interaction in combination with oxacillin (OXA) and vancomycin (VAN), and a reduction in cell viability of biofilms formed and forming by methicillin-sensitive and -resistant S. aureus. Morphological alterations, damage to the membrane, and genetic material of cells treated with promethazine were also observed. The results demonstrated that PMT can be classified as a promising antimicrobial agent for use in the antibacterial coating of long-term urinary devices.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"344-361"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulation of head-tail biofilm streamer growth based on immersed boundary method. 基于浸入边界法的头尾生物膜流体生长模拟。

IF 2.6 3区生物学

Biofouling Pub Date : 2025-04-01 Epub Date: 2025-04-14 DOI: 10.1080/08927014.2025.2490748

Yumeng Fu, Jiankun Wang, Xiaoling Wang

{"title":"Simulation of head-tail biofilm streamer growth based on immersed boundary method.","authors":"Yumeng Fu, Jiankun Wang, Xiaoling Wang","doi":"10.1080/08927014.2025.2490748","DOIUrl":"https://doi.org/10.1080/08927014.2025.2490748","url":null,"abstract":"Biofilms are subjected to various forces in the fluid field, as a result, the biofilm forms a head-tail structure known as a streamer to reduce pressure differential resistance. To characterize biofilm growth in fluid, we establish a head-tail biofilm streamer growth model based on the immersed boundary method using MATLAB software, and simulate streamer growth in various environmental conditions to explore the factors affecting its growth. Firstly, we found that a higher flow velocity makes the streamer grow faster and thereby produce more biomass. Secondly, we explored the effect of the position of nutrient source on the streamer growth, found that when the nutrient source overlaps with the streamer, its length is longer than when the nutrient source and the streamer are mismatched. Further we found that the Young's modulus of the streamer also influences its growth length. Streamers with small Young's modulus were more likely to deform, making them grow longer than the streamers with large Young's modulus. Finally, we determined the relationship between the tail length and the head diameter of the streamer through mechanical analysis, and found that there is an optimal ratio of the tail length to the head diameter which exposes the streamer to the minimum drag in the fluid field.","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":"41 4","pages":"394-406"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0