Biofouling最新文献

{"title":"Quorum quenching effects of linoleic and stearic acids on outer membrane vesicle-mediated virulence in <i>Chromobacterium violaceum</i>.","authors":"Venkatramanan Mahendrarajan, Nalini Easwaran","doi":"10.1080/08927014.2024.2446930","DOIUrl":"https://doi.org/10.1080/08927014.2024.2446930","url":null,"abstract":"<p><p><i>Chromobacterium violaceum</i> is a pathogenic bacterium that can infect humans and animals, yet the role of its outer membrane vesicles (OMVs) in mediating pathogenicity remains underexplored. This study evaluated the effects of linoleic acid (LA) and stearic acid (SA) on quorum sensing (QS)-mediated violacein production, biofilm formation, and OMV biogenesis in <i>C. violaceum</i>. Our findings revealed that 2 mM LA and 1 mM SA effectively quench QS, leading to a significant reduction in violacein production, biofilm formation, and OMV biogenesis. Gene expression analysis confirmed the downregulation of QS-related genes, including <i>cviI</i>, <i>cviR</i>, <i>vioA</i>, <i>vioB</i>, and <i>vioC</i>, in fatty acid-treated <i>C. violaceum</i>. Additionally, we assessed the antimicrobial activity of <i>C. violaceum</i>-derived OMVs on <i>Rhizobium sp.</i>, a PGPR and observed a marked reduction in bactericidal activity in the treated OMVs. This study suggests that LA and SA have potential as anti-infective agents to mitigate OMV-mediated virulence and combat antibiotic resistance in pathogens.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-11"},"PeriodicalIF":2.6,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of magnetically treated water on the survival of bacteria in biofilms.","authors":"Aidan R Foster, Erika R Stark, Luisa A Ikner, Ian L Pepper","doi":"10.1080/08927014.2024.2444379","DOIUrl":"https://doi.org/10.1080/08927014.2024.2444379","url":null,"abstract":"<p><p>The goal of this study was to evaluate if a magnetic water treatment device could be used to mitigate biofilms in water systems. Magnetic treatment was applied to water upstream of a modified Robbins device in which <i>Pseudomonas fluorescence</i> biofilms were formed. Duration of magnetic treatment, system flow rate, and field strength were varied to assess the impacts on the biofilm. A control system was concurrently established in which no magnetic treatment was applied. After treatment, the number of viable cells in the biofilm was reduced by up to 2.46 log₁₀ CFU cm^-2 depending on the operational conditions. Increased cell stress, and ultimately death, was observed during treatment as indicated by an elevated AMPi stress index. These results indicate that magnetic water treatment may be an effective technology to decrease the extent of biofilms in water systems and a reduced need for chemical treatment. A mechanism is proposed in which metabolic processes are hindered due to the magnetic field effects on ions in the water. However, a mechanistic investigation remains outside the scope of this study. Future studies should aim to characterize both the impacts of treatment on the matrix and cellular processes to determine a mechanism for the observed effects.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-13"},"PeriodicalIF":2.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel HEMA copolymer hydrogel with antifouling and anti-inflammatory activity as a promising medical device coating layer to prevent microbial adhesion.","authors":"Hadeel Abu Mahfouz, Ola Tarawneh, Lama Hamadneh, Muayad Esaifan, Sameer Al-Kouz, Ala A Alhusban, Mohammad Abu-Sini, Lana Hamdan, Buthaina Hussein, Mohammad Hailat","doi":"10.1080/08927014.2024.2442011","DOIUrl":"https://doi.org/10.1080/08927014.2024.2442011","url":null,"abstract":"<p><p>Compared to antimicrobial agents, anti-adhesive surfaces can reduce bacteria adhesion and biofilm formation in catheters, providing better selectivity, efficiency, and device life span. In this research, novel anionic surface biomaterials were created and tested to reduce microbial adhesion and colonization in medical device coating. Maleic anhydride (MA) was polymerized with 2-HEMA in varying amounts to produce a p(HEMA-<i>co</i>-MA) hydrogel copolymer. Fourier transforms infrared characterization (ATR-FTIR), thermal analysis, scanning electron microscopy with energy-dispersive X-ray spectroscopy, swelling capacity, cytotoxicity evaluation, and mixed biofilm formation ability were used to characterize the copolymer hydrogels. Hydrogels were evaluated by considering the guidance and regulations of ISO and ASTM standards. The polymers were dense, had stable cross-linking between both monomers, were non-toxic to the Human Embryonic Kidney (HEK) 293 cell line, and reduced bacterial biofilm formation statistically significantly. Furthermore, increasing the amount of MA affected <i>TGF-1</i> gene expression, where the gene expression was significantly elevated, especially at the highest percentage of MA. Furthermore, the high percentage of MA in the polymer improved the new polymer's thermal properties, film flexibility, and swelling capacity. These novel polymers could be promising materials for improving catheter biomaterial properties and modifying the surfaces of designated devices to reduce microbial infections and growth.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-11"},"PeriodicalIF":2.6,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zerumbone disrupts mixed biofilms of <i>Candida albicans</i> and <i>Streptococcus mutans</i> on acrylic resin.","authors":"Ana Luíza Gorayb Pereira, César Augusto Abreu Pereira, Luana Mendonça Dias, Janaína Habib Jorge, Ana Cláudia Pavarina","doi":"10.1080/08927014.2024.2441259","DOIUrl":"https://doi.org/10.1080/08927014.2024.2441259","url":null,"abstract":"<p><p>The efficacy of Zerumbone (ZER) against mixed biofilms of fluconazole-resistant <i>Candida albicans</i> (ATCC 96901) and <i>Streptococcus mutans</i> (UA159) was evaluated. Biofilms were cultivated on acrylic resin specimens for 48 h, with alternating supplementation of glucose and sucrose. ZER's ability to inhibit biofilm formation (pre-treatment) and eradicate mature biofilms (post-treatment) was assessed. Control groups were treated with Chlorhexidine (CHX), Nystatin (NYS), Penicillin (ATB), and distilled water. The efficacy was measured by colony forming units (CFU/mm²) counts, biomass and biofilm's matrix components quantification (water-soluble polysaccharides [WSP], alkali-soluble polysaccharides [ASPs], proteins, and extracellular DNA [eDNA]). Data were analyzed by one-way ANOVA with Tukey's or Gammes-Howell post-hoc test for normal data and Kruskal-Wallis test for data that did not meet the assumption of normality (α = 0,05). In the biofilm inhibition assay, ZER decreased total microbiota (<i>C. albicans</i> + <i>S. mutans</i>) (2.7 log₁₀; <i>p</i> < 0.005), <i>C. albicans</i> (1.4 log₁₀; <i>p</i> < 0.038) and <i>S. mutans</i> (1.9 log₁₀; <i>p</i> < 0.048) counting (vs control group), and biofilm components [insoluble proteins: 37% (<i>p</i> < 0.001); WSP: 13% (<i>p</i> < 0.042); ASP: 46% (<i>p</i> < 0.001); eDNA: 11% (<i>p</i> < 0.048)]. Post-treatment with ZER reduced total microbiota (3.2 log₁₀; <i>p</i> < 0.001), <i>C. albicans</i> (3 log₁₀; <i>p</i> < 0.001) and <i>S. mutans</i> (2 log₁₀; <i>p</i> < 0.001) counting (vs control group), and biofilm components [soluble proteins: 20% (<i>p</i> < 0.001); WSP: 20% (<i>p</i> < 0.001); ASP: 51% (<i>p</i> < 0.001); and eDNA: 33% (<i>p</i> < 0.001)]. The positive control groups demonstrated similar or lower efficacy than ZER under all experimental conditions. ZER demonstrates efficacy against mixed biofilms by reducing <i>C. albicans</i> and <i>S. mutans</i> counting and disrupting the extracellular matrix in both assays.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-16"},"PeriodicalIF":2.6,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bactericidal and antibiofilm activities of <i>Piper betle</i> extract against <i>Burkholderia pseudomallei</i>: <i>in vitro</i> and <i>in silico</i> approaches.","authors":"Komgrit Eawsakul, Wiyada Kwanhian Klangbud, Phirabhat Saengsawang, Tassanee Ongtanasup, Kunchaphorn Ratchasong, Ratchadaporn Boripun, Veeranoot Nissapatorn, Maria de Lourdes Pereira, Conny Turni, Fonthip Makkliang, Kawalin Pumbut, Watcharapong Mitsuwan","doi":"10.1080/08927014.2024.2438689","DOIUrl":"https://doi.org/10.1080/08927014.2024.2438689","url":null,"abstract":"<p><p><i>Burkholderia pseudomallei</i> biofilm is a significant virulence factor in infection. This study aimed to investigate antibacterial and antibiofilm activities of <i>Piper betle</i> extract against <i>B. pseudomallei</i>. The MIC and MBC values of the extract against the isolates were 0.5-1.0 mg/mL. At 2 × MIC, the cells showed cell shrinkage and abnormalities. At 1/2 × MIC, the extract displayed 40-71% inhibition of biofilm formation. At 8 × MIC, the extract reduced the viability of mature biofilms by 60-86%. Hydroxychavicol and eugenol, the main compounds in the extract, showed binding activity to CdpA, an enzyme implicated in biofilms as observed by <i>in silico</i> studies. Hydroxychavicol exhibited the highest affinity for CdpA, with a distance of 2.27 Å. Molecular dynamics simulations revealed that hydroxychavicol forms a stable complex with cyclic di-GMP phosphodiesterase, maintaining protein structural integrity with minimal conformational changes. The results suggested that <i>Piper betle</i> may have medicinal benefits by inhibiting biofilm-related infections.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-16"},"PeriodicalIF":2.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors affecting the spatial and temporal distribution of biofouling communities on Atlantic salmon (<i>Salmo salar</i>) farms: insights from the Broughton Archipelago, British Columbia, Canada.","authors":"Devan L Johnson, Christopher M Pearce, Mark S Flaherty, Laura L E Cowen, Morgan J Black, Sandra V Worst, Raquel A Greiter Loerzer, Kaitlin C Guitard, Matthew McGoveran, Bogdan Vornicu","doi":"10.1080/08927014.2024.2430353","DOIUrl":"https://doi.org/10.1080/08927014.2024.2430353","url":null,"abstract":"<p><p>Biofouling communities were examined at five depths at two salmon farms (Doctor Islets (DI), Wicklow Point (WP)) in British Columbia, Canada from April/May to October 2020. In addition, various water quality parameters were measured and the jellyfish numbers were quantified. Biofouling communities were mainly composed of Mollusca (primarily <i>Mytilus</i> spp.), arthropods (mostly harpacticoids), and hydroids (predominantly <i>Obelia</i> sp.), while jellyfish samples were made up mostly of medusa-form <i>Obelia</i> sp. At DI, all variables except ammonia were associated with biofouling counts, all variables except depth were associated with hydroid biomass, while only temperature, dissolved oxygen, ammonia, and nitrate were associated with jellyfish. At WP, all variables except phosphate and silica were associated with biofouling counts, only depth was associated with hydroid biomass, and only ammonia was associated with jellyfish. Insights into what environmental variables are correlated with biofouling organisms and jellyfish may assist with the development of effective mitigation strategies.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-16"},"PeriodicalIF":2.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rolling down the pilus formation of gram-positive bacteria: underlining the importance of Sortase C as a drug target.","authors":"Himanshi Kain, Ena Gupta, Prashant Sharma, Akanksha Haldiya, Vijay Kumar Srivastava, Ravi Ranjan Kumar Neeraj, Pradeep Sharma, S L Kothari, Sandip Patil, Shaowei Dong, Anupam Jyoti, Sanket Kaushik","doi":"10.1080/08927014.2024.2426167","DOIUrl":"https://doi.org/10.1080/08927014.2024.2426167","url":null,"abstract":"<p><p>Bacteria possess hair-like projections on their surface termed pili. The primary function of a pilus is to enable bacterial cell attachment to the host. Since pili are associated with cell adhesion, they play a major role in bacterial colonization and infection. Due to their important functional role, these surface appendages become ideal drug targets, hence it is essential to study the mechanism associated with pilus assembly, elongation, and attachment. Several enzymes are required for pilus biosynthesis, and their adhesion to the host. In this review paper, we have described the importance of the Sortase C (SrtC) protein which is required for pilus assembly and pilin polymerization. We also provide a detailed structural comparison of the protein from various pathogenic bacteria and highlight the importance of SrtC as a drug target. In addition to this, we have also reported structural studies of SrtC from the pathogenic bacteria <i>Enterococcus faecalis</i> using homology modelling.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"1-19"},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ozone as a promising method for controlling <i>Pseudomonas</i> spp. biofilm in the food industry: a systematic review.","authors":"Nathália Nogueira Leite, Victoria Garcia Sperandio, Eugénio da Piedade Edmundo Sitoe, Marcus Vinícius de Assis Silva, Ernandes Rodrigues de Alencar, Solimar Gonçalves Machado","doi":"10.1080/08927014.2024.2420002","DOIUrl":"10.1080/08927014.2024.2420002","url":null,"abstract":"<p><p>This study aimed to evaluate the effectiveness of ozonation in controlling <i>Pseudomonas</i> spp. biofilm in the food industry, and present possible parameters influencing this process. The study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The search was conducted in the PubMed, EMBASE, ScienceDirect, and Scopus databases. Eleven articles published between 1993 and 2023 were included in the study, indicating that the topic has been under investigation for several decades, gaining more prominence in recent years. Studies have demonstrated the antimicrobial effect of ozone under different experimental conditions, indicating that it is an effective strategy. Furthermore, they suggest that, in addition to ozone concentration and exposure time, other parameters such as the type of materials used in processing plants, hydrodynamic conditions, water temperature, and knowledge of commonly found microorganisms contribute to the effectiveness of the process aimed at reducing microbial counts. In conclusion, the available evidence suggests that ozonation in controlling <i>Pseudomonas</i> spp. can be considered a promising antimicrobial strategy. More efforts are needed to adapt the different methodologies according to each industrial reality.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"660-678"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dry biofilms on polystyrene surfaces: the role of oxidative treatments for their mitigation.","authors":"Emmanuel I Epelle, Ngozi Amaeze, William G Mackay, Mohammed Yaseen","doi":"10.1080/08927014.2024.2411389","DOIUrl":"10.1080/08927014.2024.2411389","url":null,"abstract":"<p><p><i>Candida auris</i> and <i>Staphylococcus aureus</i> are associated with a wide range of infections, as they exhibit multidrug resistance - a growing health concern. In this study, gaseous ozone, and ultraviolet-C (UVC) radiation are applied as infection control measures to inactivate dry biofilms of these organisms on polystyrene surfaces. The dosages utilised herein are 1000 and 3000 ppm.min for ozone and 2864 and 11592 mJ.cm^-2 for UVC. Both organisms showed an increased sensitivity to UVC relative to ozone exposure in a bespoke decontamination chamber. While complete inactivation of both organisms (>7.5 CFU log) was realized after 60 mins of UVC application, this could not be achieved with ozonation for the same duration. However, a combined application of ozone and UVC yielded complete inactivation in only 20 mins. For both treatment methods, it was observed that dry biofilms of <i>S. aureus</i> were more difficult to inactivate than dry biofilms of <i>C. auris</i>. Compared to dry biofilms of <i>C. auris</i>, micrographs of wet <i>C. auris</i> biofilms revealed the presence of an abundance of extracellular material after treatments. Interestingly, wet biofilms were more difficult to inactivate than dry biofilms. These insights are crucial to preventing recalcitrant and recurrent infections <i>via</i> contact with contaminated polymeric surfaces.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"772-784"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and structure of epibenthic communities across subtidal artificial hard habitats in the Bay of Cherbourg (English Channel).","authors":"Bastien Taormina, Jean-Charles Leclerc, Anne-Marie Rusig, Maxime Navon, Maël Deloor, Pascal Claquin, Jean-Claude Dauvin","doi":"10.1080/08927014.2024.2419572","DOIUrl":"10.1080/08927014.2024.2419572","url":null,"abstract":"<p><p>To inform the performance of ecological engineering designs for artificial structures at sea, it is essential to characterise their impacts on the epibenthic communities colonising them. In this context, the present study aims to compare the community structure among natural and four different artificial hard habitats with different ages and features installed in the Bay of Cherbourg (English Channel): <i>i</i>) cinder blocks and <i>ii</i>) boulders, both installed six years prior to the study, and <i>iii</i>) smooth and <i>iv</i>) rugous concrete dykes, both installed one year prior to this study. Results showed that artificial habitats installed six years ago harboured communities with functional and taxonomic diversity characteristic of mature communities but were still different from those of natural habitat. Conversely, the two dyke habitats installed one year prior to this study presented a poorly diversified community dominated by opportunistic taxa. Furthermore, while the concrete used for the two dyke habitats presented different rugosity properties, both habitats supported similar communities, suggesting that such eco-engineering measures did not affect the settlement of early colonisers. Overall, this study highlights the need for long-term monitoring to comprehensively evaluate epibenthic colonisation of artificial structures.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"847-861"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142494080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}