结合酶和阿勒颇松树精油控制不锈钢和塑料表面的阪崎克罗诺杆菌生物膜。

IF 2 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biofouling Pub Date : 2025-07-01 Epub Date: 2025-05-30 DOI:10.1080/08927014.2025.2511002

Ahmad Mohammad Ababneh, Amin N Olaimat, Murad Al-Holy, Anas Al-Nabulsi, Narmeen Al-Awwad, Saja Hamaideh, Ziad W Jaradat, Borhan Aldeen Albiss, Tareq Osaili, Richard A Holley

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引用次数: 0

摘要

本研究旨在研究阿勒颇松精油（APEO）的抗菌活性；水解酶混合物或其组合在两个连续洗涤步骤中对塑料和不锈钢表面的阪崎弧菌进行清洗。APEO对阪崎梭菌的最小抑菌浓度（MIC）为500 ~ 1000µg/ml，最小杀菌浓度（MBC）为1000 ~ 4000µg/ml。此外，APEO显示出抗膜活性，4 × MIC APEO在25°C下作用30 min，在塑料和不锈钢上分别减少1.8和1.6 log CFU/coupon。同样，这两种酶的混合物使附着在两个表面的阪崎梭菌细胞减少了1.7-2.2 log CFU/coupon。然而，用10%蛋白酶、5% α-淀粉酶和1%脂肪酶的混合酶在50°C下连续清洗30分钟，然后用4 × MIC APEO清洗30分钟，两步顺序清洗方案使两表面的阪崎弧菌生物膜比对照减少4.4-4.5 log CFU/次。

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Combined enzymes and Aleppo pine essential oil to control Cronobacter sakazakii biofilms on stainless steel and plastic surfaces.

This study aimed to investigate the antibiofilm activity of Aleppo pine essential oil (APEO); hydrolytic enzyme mixtures or their combination in two sequential washing steps against C. sakazakii on plastic and stainless steel surfaces. The minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) of APEO against C. sakazakii strains were 500-1,000 µg/ml, and 1,000-4,000 µg/ml, respectively. Further, APEO showed antibiofilm activity where 4 × MIC APEO at 25 °C for 30 min reduced C. sakazakii cells by 1.8 and 1.6 log CFU/coupon on plastic and stainless steel, respectively. Similarly, both enzyme mixtures reduced the C. sakazakii cells attached to both surfaces by 1.7-2.2 log CFU/coupon. However, the two-step sequential cleaning regime with enzyme mixture of 10% protease, 5% α-amylase, and 1% lipase at 50 °C for 30 min followed by 4 × MIC APEO for 30 min reduced C. sakazakii biofilm on both surfaces by 4.4-4.5 log CFU/coupon compared to the control.

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.