Investigation of biofilm-associated genes and biofilm formation in Non-aureus Staphylococcus (NAS) isolated from cow's milk.

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biofouling Pub Date : 2025-07-01 Epub Date: 2025-06-16 DOI:10.1080/08927014.2025.2516792
Bruna Lourenço Crippa, Pilar Lopes Mota Valente, Esther Leandra Palmeira Barros, Maria Eugênia Betim, Jaqueline Milagres de Almeida, Mirian Yuliza Rubio Cieza, Erik da Silva Pereira, Daniel Lucino Silva Dos Santos, Nathalia Cristina Cirone Silva
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Abstract

The isolation of non-aureus Staphylococcus (NAS) from the milk of both healthy cows and cows with mastitis has been frequently reported. However, there are few in-depth studies regarding their virulence profile and the ability of these microorganisms to form biofilms. Therefore, this research aimed to evaluate the biofilm formation capacity of NAS isolates from Brazilian milk. In this work, 309 NAS isolates were subjected to the Congo Red Agar (CRA) phenotypic test. Next, genotypic characterization was carried out by screening the bap, icaA, icaD, and MSCRAMMs genes: bbp, cna, ebps, eno, fib, fnbA, fnbB, clfA and clfB. Finally, ten isolates that presented the highest frequency of the genes analysed were selected to evaluate their ability to form biofilm on stainless-steel discs. t The number of cells (log10 CFU/cm2) in the biofilms was assessed at three time periods (24 h, 48 h, and 72 h) at a temperature of 25 °C. 35 NAS (11.32%) produced biofilms in the CRA test. Genotypic analysis showed the eno (38.5%) and bap (27.5%) genes were the most prevalent. In the analysis of biofilm formation on stainless steel, the factor 'growth time' had no significant effect on cell numbers. All selected isolates formed biofilm on stainless steel, and cell numbers were estimated to be in the 5.94 to 9.10 log10 CFU/m2 range. These results provide evidence that NAS isolated from milk may represent a risk to human and animal health since they carry several virulence genes and demonstrate the ability to form biofilms.

从牛奶中分离的非金黄色葡萄球菌(NAS)生物膜相关基因及生物膜形成的研究。
从健康奶牛和乳腺炎奶牛的牛奶中分离出非金黄色葡萄球菌(NAS)已被频繁报道。然而,关于它们的毒力谱和这些微生物形成生物膜的能力的深入研究很少。因此,本研究旨在评价巴西牛奶中分离的NAS生物膜形成能力。本文对309株NAS分离株进行了刚果红琼脂(CRA)表型检测。接下来,通过筛选bap、icaA、icaD和mscrms基因:bbp、cna、ebps、eno、fib、fnbA、fnbB、clfA和clfB进行基因型鉴定。最后,选出10株基因分析频率最高的菌株,以评估它们在不锈钢圆盘上形成生物膜的能力。t在25°C的温度下,在三个时间段(24小时、48小时和72小时)评估生物膜中的细胞数量(log10 CFU/cm2)。35个NAS(11.32%)在CRA试验中产生生物膜。基因型分析显示,eno(38.5%)和bap(27.5%)基因最为普遍。在不锈钢生物膜形成分析中,“生长时间”因子对细胞数量无显著影响。所有选择的菌株在不锈钢上形成生物膜,细胞数估计在5.94 ~ 9.10 log10 CFU/m2范围内。这些结果提供了证据,表明从牛奶中分离出的NAS可能对人类和动物健康构成风险,因为它们携带了几种毒力基因,并显示出形成生物膜的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biofouling
Biofouling 生物-海洋与淡水生物学
CiteScore
5.00
自引率
7.40%
发文量
57
审稿时长
1.7 months
期刊介绍: Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
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