Bruna Lourenço Crippa, Pilar Lopes Mota Valente, Esther Leandra Palmeira Barros, Maria Eugênia Betim, Jaqueline Milagres de Almeida, Mirian Yuliza Rubio Cieza, Erik da Silva Pereira, Daniel Lucino Silva Dos Santos, Nathalia Cristina Cirone Silva
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引用次数: 0
Abstract
The isolation of non-aureus Staphylococcus (NAS) from the milk of both healthy cows and cows with mastitis has been frequently reported. However, there are few in-depth studies regarding their virulence profile and the ability of these microorganisms to form biofilms. Therefore, this research aimed to evaluate the biofilm formation capacity of NAS isolates from Brazilian milk. In this work, 309 NAS isolates were subjected to the Congo Red Agar (CRA) phenotypic test. Next, genotypic characterization was carried out by screening the bap, icaA, icaD, and MSCRAMMs genes: bbp, cna, ebps, eno, fib, fnbA, fnbB, clfA and clfB. Finally, ten isolates that presented the highest frequency of the genes analysed were selected to evaluate their ability to form biofilm on stainless-steel discs. t The number of cells (log10 CFU/cm2) in the biofilms was assessed at three time periods (24 h, 48 h, and 72 h) at a temperature of 25 °C. 35 NAS (11.32%) produced biofilms in the CRA test. Genotypic analysis showed the eno (38.5%) and bap (27.5%) genes were the most prevalent. In the analysis of biofilm formation on stainless steel, the factor 'growth time' had no significant effect on cell numbers. All selected isolates formed biofilm on stainless steel, and cell numbers were estimated to be in the 5.94 to 9.10 log10 CFU/m2 range. These results provide evidence that NAS isolated from milk may represent a risk to human and animal health since they carry several virulence genes and demonstrate the ability to form biofilms.
期刊介绍:
Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion.
Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context.
Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.