Antibiofilm effect of caffeine against Listeria monocytogenes and Escherichia coli in grape and apple fruit juices.

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biofouling Pub Date : 2025-07-01 Epub Date: 2025-06-16 DOI:10.1080/08927014.2025.2515923
André Ricardo Peron Dos Santos, Bruna Camila Souza Lima, Gabriel José Couto, Luana de Carvalho, Lucília Rocha Magna, Matheus Henrique Nogueira, Mariana Lima Braga, Milena Matesco Carreteiro, Márcia Cristina Furlaneto, Luciana Furlaneto Maia
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Abstract

This study evaluated the antibiofilm activity of caffeine against Listeria monocytogenes and Escherichia coli within grape and apple juice matrices. Caffeine, a purine alkaloid recognized for its antimicrobial properties, was tested at the minimum inhibitory concentration (MIC) against planktonic and sessile cells. MIC values were established at 9.37 mM for E. coli and 37.5 mM for L. monocytogenes. Biofilm formation was evaluated through crystal violet staining, both when bacterial cells were exposed to caffeine during the initial stages of biofilm development (co-treatment) and after the biofilm had been established (post-treatment). The extracellular polymeric substances (EPS) produced within the biofilms were quantitatively measured. Bacterial viability was assessed by time-kill kinetics assays and further visualized by scanning electron microscopy (SEM). Caffeine treatment significantly inhibited biofilm formation by 98.1% for E. coli and 98.7% for L. monocytogenes, and by 98.8 and 99.0%, in co-treatment and post-treatment, respectively. No consistent correlation was observed between EPS quantity and biofilm inhibition. SEM analysis confirmed caffeine-induced structural disruption of the biofilm matrix and damage to bacterial cell integrity. This investigation introduces a novel application of caffeine as an anti-biofilm agent in unpreserved fruit juice systems and demonstrates, for the first time, its efficacy against foodborne pathogens.

咖啡因对葡萄和苹果果汁中单核增生李斯特菌和大肠杆菌的抗菌作用。
本研究评价了咖啡因对葡萄和苹果汁基质中单核细胞增生李斯特菌和大肠杆菌的抗菌膜活性。咖啡因是一种以抗菌特性而闻名的嘌呤生物碱,在最低抑制浓度(MIC)下对浮游细胞和无根细胞进行了测试。大肠杆菌的MIC值为9.37 mM,单核增生乳杆菌为37.5 mM。当细菌细胞在生物膜发育的初始阶段(共处理)和生物膜建立后(后处理)暴露于咖啡因时,通过结晶紫染色评估生物膜的形成。定量测定生物膜内产生的胞外聚合物质(EPS)。通过时间杀伤动力学试验评估细菌活力,并通过扫描电子显微镜(SEM)进一步观察。在共处理和后处理中,咖啡因对大肠杆菌和单核增生乳杆菌的生物膜形成的抑制作用分别为98.1%和98.7%,分别为98.8%和99.0%。EPS数量与生物膜抑制作用之间没有一致的相关性。扫描电镜分析证实了咖啡因引起的生物膜基质的结构破坏和细菌细胞完整性的损害。本研究介绍了咖啡因作为一种抗生物膜剂在未保存果汁系统中的新应用,并首次证明了其对食源性病原体的功效。
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来源期刊
Biofouling
Biofouling 生物-海洋与淡水生物学
CiteScore
5.00
自引率
7.40%
发文量
57
审稿时长
1.7 months
期刊介绍: Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
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