Ariel Gonzalez, Alejandro Miñán, Eduardo Prieto, Patricia Schilardi, Natalia S Fagali, Mónica Fernández Lorenzo de Mele
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引用次数: 0
摘要
耐多药细菌生物膜的兴起对生物医学应用提出了重大挑战，需要创新和环保的解决方案。在这项研究中，使用两种异构体植物化合物：香芹酚（Carv-Ti-NL）和百里香酚（TOH-Ti-NL）在钛（Ti）表面设计了杀菌纳米层（NLs）。这些NLs是通过一个简单的一步自组装过程制造的。对金黄色葡萄球菌均表现出较强的抗生物膜和杀菌活性。结果表明，TOH-Ti-NL具有较好的成骨作用，而成纤维细胞在TOH-Ti-NL上的粘附能力降低，而在car - ti - nl上的增殖能力增强。衰减全反射傅里叶变换红外光谱（ATR-FTIR）证实了Carv和TOH在Ti上自发氧化成酮结构。与Carv-Ti-NL相比，TOH-Ti-NL还表现出更高的表面粗糙度，与骨整合改善有关，并且释放率更高。两种涂层都能在24小时内根除细菌。它们的早期有效性强调了它们在钛植入物上预防感染的潜力。因此，TOH-Ti-NL有望用于骨相关应用，而car - ti - nl可能更适合成纤维细胞生长，为各种生物医学应用提供量身定制的特性。
Antibiofilm and osteogenic nanocoatings: a comparative study of self-assembled layers of the isomers carvacrol and thymol on titanium biomaterials.
The rise of multidrug-resistant bacterial biofilms presents a significant challenge in biomedical applications, demanding innovative and eco-friendly solutions. In this study, bactericidal nanolayers (NLs) were engineered on titanium (Ti) surfaces using two isomeric phytocompounds: carvacrol (Carv-Ti-NL) and thymol (TOH-Ti-NL). These NLs were fabricated via a simple, one-step self-assembly process. Both exhibited strong anti-biofilm and bactericidal activity against Staphylococcus aureus. TOH-Ti-NL proved superior for osteogenesis, while fibroblasts showed reduced adhesion on TOH-Ti-NL but enhanced proliferation on Carv-Ti-NL. Attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) spectroscopy confirmed spontaneous oxidation of Carv and TOH on Ti into ketonic structures. TOH-Ti-NL also displayed higher surface roughness, linked to improved osseointegration, and a higher release rate than Carv-Ti-NL. Both coatings eradicated bacteria within 24 h. Their early effectiveness underscores their potential for infection prevention on Ti implants. Thus, TOH-Ti-NL shows promise for bone-related applications, whereas Carv-Ti-NL may be better suited for fibroblast growth, offering tailored properties for diverse biomedical applications.
期刊介绍：
Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion.
Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context.
Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
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