In vitro study of essential oils encapsulated in chitosan microparticles against Histoplasma capsulatum and their pathogenicity in Caenorhabditis elegans.

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biofouling Pub Date : 2025-02-01 Epub Date: 2025-02-06 DOI:10.1080/08927014.2025.2453184

Anderson da Cunha Costa, Mirele Rodrigues Fernandes, Augusto Feynman Dias Nobre, Maria Gleiciane Rocha, Jacó Ricarte Lima de Mesquita, Rosemeyre Souza Freire, Andre Jalles Monteiro, Rodrigo Silveira Vieira, Raimunda Sâmia Nogueira Brilhante

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Abstract

Histoplasmosis, caused by Histoplasma capsulatum, poses risks for immunocompromised individuals. With limited therapeutic options, this study explores microparticles as antimicrobial delivery systems for Cymbopogon flexuosus and Pelargonium graveolens essential oils against H. capsulatum. The broth microdilution assay showed MICs of 32 to 128 µg/mL in filamentous phase and 8 to 64 µg/mL in yeast phase. Combining microparticles with antifungal drugs demonstrated synergistic effects in both filamentous and yeast-like forms with amphotericin B or itraconazole. Chitosan microparticles reduced H. capsulatum biofilm biomass and metabolic activity by about 60% at 512 µg/mL. In vivo evaluation with Caenorhabditis elegans showed H. capsulatum caused over 90% mortality. These findings highlight the potential use of chitosan microparticles as a delivery system for essential oils against H. capsulatum, especially in combination with other compounds.

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壳聚糖微颗粒精油体外抗荚膜组织浆体作用及其对秀丽隐杆线虫致病性的研究。

由荚膜组织浆体引起的组织浆菌病对免疫功能低下的个体构成风险。在有限的治疗选择下，本研究探索了微颗粒作为对弯曲Cymbopogon flexosus和Pelargonium graveolens精油对荚膜荚膜菌的抗菌递送系统。肉汤微量稀释试验显示，丝状菌期mic为32 ~ 128µg/mL，酵母期mic为8 ~ 64µg/mL。将微颗粒与抗真菌药物结合，与两性霉素B或伊曲康唑在丝状和酵母样形式中均表现出协同作用。壳聚糖微颗粒在512µg/mL浓度下，可使荚膜荚膜生物量和代谢活性降低约60%。对秀丽隐杆线虫的体内评价显示，荚膜荚膜梭菌致死率超过90%。这些发现突出了壳聚糖微粒作为精油抗荚膜荚膜菌递送系统的潜在用途，特别是与其他化合物结合使用时。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.