Biofouling最新文献

{"title":"Antibiofilm biosynthetic terpene derivatives against clinically and food-relevant bacteria, including synergistic interactions with <b>β</b>-lactam antibiotics: quantum chemical calculations, molecular docking and ADMET study.","authors":"Rafaela Mesquita Bastos Cavalcante, Ana Maria Aguiar Nascimento, Rhanna Karyne Rodrigues Muniz, Jéssica Híade Silva Cristino, Victor Moreira de Oliveira, Emmanuel Silva Marinho, Marcia Machado Marinho, Francisco Nithael Melo Lucio, Jesyka Macedo Guedes, Glaydson Leandro Farias Mendonça, Thiago Dos Santos Francisco, Francisco Ferdinando Mesquita Cajazeiras, Jaíza Maria Lima Dias, Victor Alves Carneiro, Hélcio Silva Dos Santos","doi":"10.1080/08927014.2026.2656304","DOIUrl":"10.1080/08927014.2026.2656304","url":null,"abstract":"<p><p>The indiscriminate use of antibiotics and biofilm formation have contributed to the emergence of resistant infections. Natural compounds such as limonene and its derivatives have gained attention due to their therapeutic potential and lower risk of resistance. This study evaluated the antibacterial and antibiofilm activity of limonene isomers ((R)- and (S)-limonene) and their derivatives ((S)-perillyl alcohol and (S)-perillaldehyde), as well as their combined effects with β-lactam antibiotics against Gram-positive and Gram-negative strains of clinical and food relevance. Disk diffusion, MIC, MBC, growth curve, biofilm quantification (biomass and cell viability), and checkerboard assays were performed. (S)-limonene, (S)-perillyl alcohol, and (S)-perillaldehyde showed bactericidal activity, whereas (R)-limonene was bacteriostatic. Structural and electronic analyses indicated that oxygenation and substituents modify electron density, polarity, and reactivity, influencing biological activity. All compounds delayed bacterial growth and significantly reduced biofilm biomass and cell viability, with S-isomers achieving reductions of up to 90%. Combinatory analysis revealed synergistic and additive effects with oxacillin, benzylpenicillin, ampicillin, and ceftriaxone, particularly against MRSA and <i>Klebsiella pneumoniae</i>. In silico docking suggested potential inhibition of Gyrase B, thymidylate kinase, and topoisomerase through interactions with active-site residues. ADMET predictions indicated favorable permeability and absorption, especially for S-PA and R-LMN. These findings highlight the potential of limonene derivatives as alternative or adjunct antimicrobial agents against biofilm-associated infections.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"515-537"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Achieving bacteria eradication on titanium implants: synergistic effect of tannic acid coating and levofloxacin.","authors":"Valentina Chiara Cajiao Checchin, Alejandro Miñán, Eduardo Prieto, Natalia Soledad Fagali, Mónica Fernández Lorenzo de Mele","doi":"10.1080/08927014.2026.2653583","DOIUrl":"10.1080/08927014.2026.2653583","url":null,"abstract":"<p><p>Titanium (Ti)-based implants may cause implant-related infections (IRIs) due to contamination before or during surgery. Tannic acid (TA) coatings offer a promising strategy to prevent IRIs owing to their antibacterial properties. TA binds strongly to metal oxide surfaces, forming stable self-assembled layers. We developed a simple method based on TA adsorption onto rough Ti substrates combined with a low dose of levofloxacin (LEV) to eradicate <i>Staphylococcus aureus</i> biofilms, the species most frequently associated with orthopedic implants. Physicochemical analyses (ATR-FTIR, contact angle, electrochemistry, AFM) confirmed TA deposition, increased hydrophilicity, and altered surface morphology. Microbiological assays revealed a two-log reduction in viable bacteria on TA-coated surfaces and a three-log reduction on LEV-treated surfaces compared to untreated Ti. Notably, the combined TA functionalization + LEV treatment exhibited a synergistic effect, resulting in effective bacterial eradication. These results highlight the potential of this approach as a preventive strategy for IRIs on Ti implants.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"502-514"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the impact of dust deposition and CO₂ level on biofilm development causing biodeterioration of historic buildings and monuments in India.","authors":"Riya Singh, Ilma Khan, Riya Sharma, Saurabh Mishra, Lalit Kumar Pandey","doi":"10.1080/08927014.2026.2645567","DOIUrl":"10.1080/08927014.2026.2645567","url":null,"abstract":"<p><p>The deterioration of buildings and historic monuments due to phototrophic biofilms, dust, and carbon dioxide concentration is an increasingly significant global issue, but remains insufficiently studied. In this research, phototrophic biofilms were collected from nine sampling sites, which were categorized as control, moderately contaminated, and severely contaminated based on the air quality index and canopy cover. Severely contaminated sites showed higher temperatures, CO₂ levels, and dust accumulation, but lower humidity. Cyanobacteria dominated all biofilm samples, followed by fungal hyphae and spores. Green algae and diatoms contributed minimally. Healthy diatom frustules decreased sharply with increasing contamination, from the control sites to severely contaminated sites. Regression analysis revealed significant positive correlations between cyanobacterial abundance and both CO₂ levels and dust deposition, indicating that increased air pollutants promote the growth of cyanobacteria and diatoms in phototrophic biofilms, further highlighting their potential as a sensitive bioindicator of environmental contamination affecting cultural heritage sites.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"459-473"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pore formation in a Portland cement during two-year microbial fouling under conditions simulating deep radioactive waste geological repository.","authors":"Elena Abramova, Nadezhda Popova, Grigory Artemiev, Ivan Zel, Bulat Bakirov, Murat Kenessarin, Igor Chuprakov, Alexey Safonov","doi":"10.1080/08927014.2026.2649938","DOIUrl":"10.1080/08927014.2026.2649938","url":null,"abstract":"<p><p>Changes in pore formation, strength characteristics and mineral composition of Portland cement M500 during biofouling under conditions simulating a deep geological radioactive waste repository were evaluated. The biocide polyhexamethylene guanidine (PHMG) was added to inhibit microbial growth. After two years, microbial activity was found to be a key determinant of the cement's strength characteristics due to the dissolution of primary mineral phases (portlandite, C3S, C2S and C2FA) and the formation of calcite. The volume fraction of cracks increased by an average of 25 times in all samples. Notably, the addition of PHMG did not significantly affect the porosity of the cement, and its compressive strength parameters showed no correlation with pore and crack content during long-term storage. The observed strength increase with PHMG is likely attributable to the formation of an additional polymer skeleton within the cement matrix.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"486-501"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold atmospheric plasma and plasma‑treated saline in the inactivation of <i>Staphylococcus aureus</i> biofilms: structural and physicochemical insights from multimodal analyses.","authors":"Tamer Akan, E Serhat Yavaş, Ahmet Çabuk, Pınar Aytar Çelik, İlknur Dağ, Çağrı Durmuş","doi":"10.1080/08927014.2026.2663011","DOIUrl":"10.1080/08927014.2026.2663011","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> biofilms pose significant challenges in medical and industrial settings by exhibiting high resistance to conventional treatments. Although cold atmospheric plasma (CAP) shows promise as an antibacterial agent, the mechanisms of interaction with bacteria, particularly in saline environments, remain poorly understood. <i>S. aureus</i> biofilms were exposed to Argon CAP, and morphological and metabolic changes were evaluated using confocal laser scanning microscopy, field emission scanning electron microscopy, Fourier transform infrared spectroscopy, atomic force microscopy, and XTT fluorescence. Planktonic cells were further examined by transmission electron microscopy. The findings revealed significant biofilm disruption with 94% metabolic inactivation after 300 s. To distinguish direct plasma effects, plasma-treated saline was tested and demonstrated to inactivate within 30 min. Optical emission spectroscopy detected intense Na emission (589 nm) during plasma-saline interactions; however, this observation is reported as a spectral feature rather than a mechanistic conclusion, and further experiments are required to clarify its potential relevance.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"554-570"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-phase adhesive secretion in the stalked barnacle <i>Capitulum mitella</i>.","authors":"Ziquan Zhou, Zewen Zheng, Xiuqin Bai, Xiaozhen Rao, Gang Lin","doi":"10.1080/08927014.2026.2649225","DOIUrl":"10.1080/08927014.2026.2649225","url":null,"abstract":"<p><p>Barnacles are a highly diverse group that includes sessile, stalked, and parasitic forms, all capable of adhering strongly to wet surfaces. While acorn barnacle adhesion has been well studied, the mechanisms in stalked barnacles remain poorly understood. Herein, the adhesive of <i>Capitulum mitella</i> is investigated, revealing a two-phase secretion: a fluid-phase mixture extruded through glandular structures and a solidified adhesive with distinct lamellar architecture. The outer layers of adhesive can resist environmental stress, while the inner fibrous network contributes to cohesion and viscoelasticity. These components differ markedly in morphology and chemical composition. Raman spectroscopy, FTIR, and thioflavin T staining confirm the absence of amyloid-like β-sheet structures, in contrast to previously reported amyloid-based architectures in acorn barnacle adhesives. The results highlight species-specific adhesive strategies in stalked barnacles and offer insights relevant to the development of bioinspired adhesives and antifouling technologies.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"474-485"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrate modulates microbial interaction and biocorrosion activities of acid producing bacteria.","authors":"Tekle Tafese Fida, Taylor Sherman, Marie Hall, Rebekah Wilson, Scott Leleika, Karen Crippen","doi":"10.1080/08927014.2026.2661971","DOIUrl":"10.1080/08927014.2026.2661971","url":null,"abstract":"<p><p>Acid-producing bacteria (APB) play significant role in pipeline corrosion by producing corrosive metabolites. Current APB mitigation strategies are often unsuccessful, costly, or not environmentally friendly. In this study, nitrate was used to manipulate microbial interaction and control APB activities. Microbial diversity was manipulated by subsampling and culturing sufficient replicates of serially diluted subsamples to achieve random distribution in the absence or presence of nitrate. Microbial activities were evaluated by monitoring changes in pH, corrosion rate, production of organic acids, changes in community composition, and functional gene abundance. The study identified a change in microbial community assemblage with decreased abundance of <i>Clostridium</i> upon nitrate addition, resulting in limited APB activities such as acid production and biocorrosion. Nitrate resulted in about a 3.5-fold decrease in carbon steel weight loss corrosion induced by APB but did not affect microbial growth and abundance of metabolic genes, suggesting that the inhibitory effect is likely due to changes in redox potential of culture media or increased growth of APB competitors. The study also identified the importance of nitrate as an economically viable and environmentally sustainable approach to mitigate APB-induced biocorrosion.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"538-553"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of biofouling characteristics and issues for offshore renewable energy industry.","authors":"Aurélie Portas, Jean-François Briand, Guillaume Damblans, Nolwenn Quillien","doi":"10.1080/08927014.2026.2630946","DOIUrl":"10.1080/08927014.2026.2630946","url":null,"abstract":"<p><p>The development of offshore renewable energy (ORE) is accelerating to reach global decarbonisation objectives, but it faces technical and ecological challenges, including biofouling. This review synthesises current knowledge on biofouling colonising ORE structures, focusing on: 1) its technical impacts (increased hydrodynamic drag, corrosion, decreased component lifespan), and 2) its environmental impacts (modification of habitat, reef effect, non-native species dispersal). Special focus is given to floating systems, such as floating wind turbines, where biofouling significantly influences mooring lines and dynamic cables. Despite increasing interest in the field, many uncertainties remain, notably the lack of <i>in situ</i> offshore data, limited understanding of biofouling-structure interactions, and absence of standardised measurement protocols. The review discusses main knowledge gaps in offshore fouling dynamics and proposes future research perspectives to improve maintenance strategies and environmental management. Understanding these processes is essential for the long-term sustainability of ORE technologies and their integration into marine ecosystems.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"339-360"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147301597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piperine inhibits biofilm formation in co-cultures of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>.","authors":"Sharmistha Das, Ritwik Roy, Moumita Malik, Payel Paul, Poulomi Chakraborty, Sudipta Chatterjee, Debasish Maiti, Prosun Tribedi","doi":"10.1080/08927014.2026.2630947","DOIUrl":"10.1080/08927014.2026.2630947","url":null,"abstract":"<p><p>Polymicrobial biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> pose serious clinical challenges due to their persistence, metabolic adaptability, and antibiotic tolerance. The present study investigated the ability of these two bacteria to co-exist and form mixed-species biofilms and evaluated the antibiofilm potential of piperine, a plant-derived alkaloid from <i>Piper nigrum</i>. Co-existence of <i>S. aureus</i> and <i>P. aeruginosa</i> was confirmed by enumeration of colony-forming units, growth kinetics, cross-streaking, metabolic fingerprinting, Gini coefficient analysis, and antibiotic susceptibility. Sub-minimum inhibitory concentrations of piperine significantly inhibited mixed-species biofilm formation, as demonstrated by biochemical, microbiological and microscopic analysis. Piperine treatment led to noticeable reductions in biofilm biomass, exopolysaccharide content, total protein content, metabolic activity and extracellular DNA. Mechanistic investigations revealed that piperine impaired biofilm-forming determinants by reducing cellular co-aggregation and cell surface hydrophobicity, inducing intracellular reactive oxygen species (ROS) accumulation, and increasing membrane permeability. Significantly, piperine effectively disrupted pre-established mixed-species biofilms, and its antibiofilm efficacy was further validated in a catheter model, highlighting its relevance against device-associated infections. Collectively, these findings demonstrate that piperine inhibits and disintegrates <i>S. aureus</i>-<i>P. aeruginosa</i> biofilms through diverse mechanisms, positioning it as a promising phytochemical for managing biofilm-associated infections.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"411-428"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147301588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequential attachment and <i>Listeria</i> predominance under turbulent flow.","authors":"Krisha Pant, Jon Palmer, Steve Flint","doi":"10.1080/08927014.2026.2630941","DOIUrl":"10.1080/08927014.2026.2630941","url":null,"abstract":"<p><p>The exploration of multispecies biofilms has provided significant insight into how bacteria interact in natural environments. However, crucial information is still lacking when the assumption that all the bacteria approach the substrate simultaneously during multispecies biofilm formation is challenged. The objective of this study was to analyse the multispecies biofilm formed by industrially relevant bacteria, including <i>Pseudomonas fluorescens</i> and <i>Listeria monocytogenes</i>, focusing on sequential colonisation under turbulent flow and static conditions. Under flow conditions, the attachment of <i>Listeria</i> on preformed <i>Pseudomonas</i> biofilm was significantly higher (<i>p</i> < 0.001) and reached its highest cell concentration (8.9 log CFUcm^-2) earlier (by 24 h) compared to control (sterile stainless-steel surfaces) and co-inoculated (with <i>P. fluorescens</i>) conditions. The conditioning of stainless-steel surfaces with freeze-dried and rehydrated exopolysaccharides (0-27.5 µgml^-1) extracted from <i>P. fluorescens</i> did not affect <i>L. monocytogenes</i> attachment, indicating the importance of biofilm architecture. The cell concentration of <i>P. fluorescens</i> was not affected by either the flow rate or the colonisation order in a multispecies biofilm. Overall, the sequence of colonisation affects biofilm formation in multispecies biofilms under turbulent flow and is an important variable in studies of the community lifestyle of multispecies biofilms.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"375-390"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}