Bactericidal and antibiofilm activities of Piper betle extract against Burkholderia pseudomallei: in vitro and in silico approaches.

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biofouling Pub Date : 2024-12-17 DOI:10.1080/08927014.2024.2438689

Komgrit Eawsakul, Wiyada Kwanhian Klangbud, Phirabhat Saengsawang, Tassanee Ongtanasup, Kunchaphorn Ratchasong, Ratchadaporn Boripun, Veeranoot Nissapatorn, Maria de Lourdes Pereira, Conny Turni, Fonthip Makkliang, Kawalin Pumbut, Watcharapong Mitsuwan

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Abstract

Burkholderia pseudomallei biofilm is a significant virulence factor in infection. This study aimed to investigate antibacterial and antibiofilm activities of Piper betle extract against B. pseudomallei. The MIC and MBC values of the extract against the isolates were 0.5-1.0 mg/mL. At 2 × MIC, the cells showed cell shrinkage and abnormalities. At 1/2 × MIC, the extract displayed 40-71% inhibition of biofilm formation. At 8 × MIC, the extract reduced the viability of mature biofilms by 60-86%. Hydroxychavicol and eugenol, the main compounds in the extract, showed binding activity to CdpA, an enzyme implicated in biofilms as observed by in silico studies. Hydroxychavicol exhibited the highest affinity for CdpA, with a distance of 2.27 Å. Molecular dynamics simulations revealed that hydroxychavicol forms a stable complex with cyclic di-GMP phosphodiesterase, maintaining protein structural integrity with minimal conformational changes. The results suggested that Piper betle may have medicinal benefits by inhibiting biofilm-related infections.

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瓜蒌提取物对假马勒伯克霍尔德氏菌的杀菌和抗生物膜活性：体外和硅学方法。

假杆菌伯克霍尔德菌生物膜是感染的重要毒力因子。本研究旨在研究红椒提取物对假芽孢杆菌的抑菌活性和抗膜活性。提取物对分离菌的MIC和MBC值为0.5 ~ 1.0 mg/mL。在2倍MIC下，细胞出现细胞萎缩和异常。在1/2 × MIC下，提取物对生物膜形成的抑制作用为40-71%。在8倍MIC下，提取物使成熟生物膜的活力降低了60-86%。羟基丁香酚和丁香酚是提取物中的主要化合物，显示出与CdpA结合的活性，CdpA是一种与生物膜有关的酶，由硅研究观察到。羟基chavicol对CdpA的亲和力最高，距离为2.27 Å。分子动力学模拟表明，羟基chavicol与环二gmp磷酸二酯酶形成稳定的配合物，以最小的构象变化保持蛋白质结构的完整性。结果提示，琵琶鱼可能通过抑制生物膜相关感染而具有药用价值。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.