Bioscience of Microbiota, Food and Health最新文献

{"title":"Oral administration of <i>Lacticaseibacillus casei</i> ATCC393 promotes angiogenesis by enhancing neutrophil activity in a murine hind-limb ischemia model.","authors":"Suguru Saito,&nbsp;Musin Kelel","doi":"10.12938/bmfh.2022-038","DOIUrl":"https://doi.org/10.12938/bmfh.2022-038","url":null,"abstract":"<p><p>Angiogenesis is a highly regulated biological event and requires the participation of neutrophils, which are innate immune cells, to initiate the systematic responses. Some strains of lactic acid bacteria (LAB) can be used for probiotics that provide functional modifications in our immune systems. Here, we show that oral administration of <i>Lacticaseibacillus casei</i> ATCC393 promoted inflammatory angiogenesis accompanied by enhanced neutrophil activity. Heat-killed <i>L. casei</i> (HK-LC) administration improved angiogenesis in a murine hind-limb ischemia (HLI) model. The recruitment and activity of neutrophils were enhanced by HK-LC administration under the HLI conditions. Our results provide novel evidence of an immunological contribution of LAB uptake in the prevention of or recovery from cardiovascular diseases.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"42 1","pages":"94-99"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6a/2e/bmfh-42-094.PMC9816049.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9103336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canna starch improves immune functions and the intestinal environment in mice.","authors":"Mamoru Tanaka,&nbsp;Ayaka Koida,&nbsp;Akira Miyazaki,&nbsp;Kazushi Tabata,&nbsp;Yuichiro Takei,&nbsp;Yoshihumi Tanimoto,&nbsp;Mami Kawamura,&nbsp;Masafumi Tsuzuki,&nbsp;Haruka Takahashi,&nbsp;Tetsu Yano,&nbsp;Hiroyuki Watanabe","doi":"10.12938/bmfh.2022-068","DOIUrl":"https://doi.org/10.12938/bmfh.2022-068","url":null,"abstract":"<p><p>The present study was conducted to elucidate the dietary effects of canna starch on the immune functions and intestinal luminal environment in mice. The amylose and resistant starch characteristics were determined for six types of starch, including edible canna. Canna starch was found to be higher in amylose and resistant starch compared with the other starches. BALB/c mice were fed 3.16% (low-canna group) and 6.32% (high-canna group) canna starch for 2 weeks, and then intestinal parameters were measured. Fecal IgA and mucin levels were markedly elevated by canna starch intake. IgA levels in serum and spleen lymphocytes were elevated by canna starch intake in the high-canna group, but not in the low-canna group. When the mice were fed canna starch, the cecum weight increased, and the pH in the cecum decreased. The high-canna group had significantly increased levels of <i>Clostridium</i> subcluster XIVa lactic acid, acetic acid, and n-butyric acid in the cecum compared with the control group. These results suggested that canna starch supplementation changed the intestinal microbiota and enhanced the intestinal immune and barrier functions and cecal organic acids in mice.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"42 2","pages":"131-137"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/f2/bmfh-42-131.PMC10067328.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9252566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of circadian clock and claudin regulations on inulin-induced calcium absorption in the mouse intestinal tract.","authors":"Kazuto Shiga,&nbsp;Atsushi Haraguchi,&nbsp;Hiroyuki Sasaki,&nbsp;Yu Tahara,&nbsp;Kanami Orihara,&nbsp;Shigenobu Shibata","doi":"10.12938/bmfh.2022-029","DOIUrl":"https://doi.org/10.12938/bmfh.2022-029","url":null,"abstract":"<p><p>Dietary calcium supplementation has been shown to be an effective adjunct therapy in an inflammatory bowel disease model. Soluble dietary fiber reduces intestinal pH and is known to enhance calcium absorption. Although many circadian clock regulations of nutrient absorption in the intestinal tract have been reported, the effects of clock regulation on calcium absorption have yet to be understood. In this study, we investigated the timing of efficient calcium intake by measuring urinary calcium excretion in mice. The diurnal variations in channel-forming tight junctions (claudins) were detected in both the jejunum and ileum. Following 2 days of feeding with a Ca²⁺-free diet, Ca²⁺-containing diets with or without soluble fiber (inulin) were fed at specific timings, and urine was subsequently examined every 4 hr. There was an evident increase in urinary calcium concentration when the inulin diet was fed at the beginning of the resting period. The <i>Claudin 2</i> (<i>Cldn2</i>) expression level also showed a significant day-night change, which seemed to be a mechanism for the increased calcium excretion after inulin intake. This diurnal rhythm and enhanced <i>Cldn2</i> expression were abolished by disruption of the suprachiasmatic nucleus, the central clock in the hypothalamus. This study suggests that intestinal calcium absorption might be modulated by the circadian clock and that the intake of inulin is more effective at the beginning of the resting period in mice.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"42 2","pages":"114-123"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/45/92/bmfh-42-114.PMC10067330.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9258286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bacillus subtilis</i> TO-A extends the lifespan of <i>Caenorhabditis elegans</i>.","authors":"Ryuichi Saito,&nbsp;Naoki Sato,&nbsp;Yoichi Okino,&nbsp;Dian-Sheng Wang,&nbsp;Genichiro Seo","doi":"10.12938/bmfh.2022-057","DOIUrl":"https://doi.org/10.12938/bmfh.2022-057","url":null,"abstract":"<p><p><i>Clostridium butyricum</i> TO-A, <i>Enterococcus faecium</i> T-110, and <i>Bacillus subtilis</i> TO-A are sold as oral probiotic preparations and reportedly exhibit many beneficial effects on the health of hosts, including humans and livestock. In this study, we compared the ability of these clinically applied probiotic bacteria with <i>Escherichia coli</i> OP50 in extending the lifespan of <i>Caenorhabditis elegans</i>. To compare the <i>C. elegans</i> lifespan-extending effects of the three bacteria, experiments were performed using a nematode growth medium containing a small amount of trypticase soy agar. The maximum lifespans of worms fed <i>C. butyricum</i> TO-A, <i>E. faecium</i> T-110, or <i>B. subtilis</i> TO-A increased by 11, 12, and 26%, respectively, compared with worms fed <i>E. coli</i> OP50. In addition, we conducted a metabolomic analysis of methanol extracts of <i>B. subtilis</i> TO-A cells, which exhibited the strongest lifespan-extending effect on <i>C. elegans</i> among the probiotic bacteria tested in this study. As a result, 59 candidate substances involved in extending the lifespan of <i>C. elegans</i> were identified in <i>B. subtilis</i> TO-A cells.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"42 2","pages":"124-130"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/e9/bmfh-42-124.PMC10067327.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9258287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety evaluation of a heat-treated &lt;i&gt;Bifidobacterium bifidum &lt;/i&gt;OLB6378 concentrate","authors":"Misato TSUBOI, Masaki TERAHARA, Masashi NAGATA, Toyohisa KATSUMATA, Takahiro ISHII, Masayuki KATO, Yoshitaka NAKAMURA","doi":"10.12938/bmfh.2023-044","DOIUrl":"https://doi.org/10.12938/bmfh.2023-044","url":null,"abstract":"Several bacterial strains, including probiotic strains, have undergone evaluations for their safety and potential beneficial health effects. Some of these strains have been introduced into various markets, including that for infant products. However, certain probiotic strains have been linked to serious infections in infants, such as septicemia and meningitis. Given this, it is crucial to assess the safety of each probiotic strain, including those of Bifidobacterium, which is a common genus of probiotics. One such strain, Bifidobacterium bifidum OLB6378 (NITE BP-31), referred to as OLB6378 hereafter, has been selected for use in infants. To determine its genotoxicity and general toxicity potential, a heat-treated OLB6378 concentrate was subjected to various tests, including the bacterial reverse mutation test, in vitro chromosome aberration test, in vivo micronucleus test, and single- and 90-day oral gavage toxicity studies in rats. No significant differences were observed compared with negative controls in any of genotoxicity tests. The single-dose toxicity study employed dose levels of 560, 1,693, and 5,092 mg/kg, representing the total solid contents of culture concentrates containing OLB6378 (equivalent to 8.1 × 1011, 2.4 × 1012, and 7.4 × 1012 cells/kg of Bifidobacterium, respectively). In the 90-day toxicity study, dose levels of 280, 853, and 2,546 mg/kg/day were used (equivalent to 4.0 × 1011, 1.2 × 1012, and 3.7 × 1012 cells/kg/day, respectively). Importantly, the heat-treated OLB6378 concentrate did not induce any signs of toxicity in any of the conducted toxicity studies. In conclusion, the heat-treated OLB6378 concentrate exhibited no genotoxicity potential, and the no-observed-adverse-effect level in the 90-day toxicity study was determined to be 2,546 mg/kg/day (equivalent to 3.7 × 1012 cells/kg/day). This suggests that heat-treated OLB6378 can be safely utilized as a food source.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134890191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fucosylated human milk oligosaccharide-utilizing bifidobacteria regulate the gut organic acid profile of infants","authors":"Kana YAHAGI","doi":"10.12938/bmfh.2023-069","DOIUrl":"https://doi.org/10.12938/bmfh.2023-069","url":null,"abstract":"Bifidobacteria are the predominant bacteria in the infant gut and have beneficial effects on host physiology. Infant cohort studies have demonstrated that a higher abundance of bifidobacteria in the gut is associated with a reduced risk of disease. Recently, bifidobacteria-derived metabolites, such as organic acid, have been suggested to play crucial roles in host physiology. This review focuses on an investigation of longitudinal changes in the gut microbiota and organic acid concentrations over 2 years of life in 12 Japanese infants and aims to identify bifidobacteria that contribute to the production of organic acid in healthy infants. Acetate, lactate, and formate, which are rarely observed in adults, are characteristically observed during breast-fed infancy. Bifidobacterium longum subspecies infantis and the symbiosis of Bifidobacterium bifidum and Bifidobacterium breve efficiently produce these organic acids through metabolization of human milk oligosaccharide (HMO) with different strategies. These findings confirmed that HMO-utilizing bifidobacteria play an important role in regulating the gut organic acid profiles of infants.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135319744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Enterococcus faecium</i> 129 BIO 3B is classified as <i>Enterococcus lactis</i> 129 BIO 3B.","authors":"Kiyofumi Ohkusu","doi":"10.12938/bmfh.2022-088","DOIUrl":"https://doi.org/10.12938/bmfh.2022-088","url":null,"abstract":"<p><p><i>Enterococcus faecium</i> 129 BIO 3B is a lactic acid bacterium that has been safely used as a probiotic product for over 100 years. Recently, concerns about its safety have arisen because some species of <i>E. faecium</i> belong to the vancomycin-resistant enterococci. The groups of <i>E. faecium</i> with less pathogenic potential have been split into a separate species (<i>Enterococcus lactis</i>). In this study, I investigated the phylogenetic classification and safety of <i>E. faecium</i> 129 BIO 3B as well as <i>E. faecium</i> 129 BIO 3B-R, which is naturally resistant to ampicillin. Mass spectrometry and basic local alignment search tool analysis using specific gene regions failed to differentiate 3B and 3B-R into <i>E. faecium</i> or <i>E. lactis</i>. However, multilocus sequence typing successfully identified 3B and 3B-R as the same sequence types as <i>E. lactis</i>. Overall genome relatedness indices showed that 3B and 3B-R have high degrees of homology with <i>E. lactis</i>. Gene amplification was confirmed for 3B and 3B-R with <i>E. lactis</i> species-specific primers. The minimum inhibitory concentration of ampicillin was confirmed to be 2 µg/mL for 3B, which is within the safety standard for <i>E. faecium</i> set by the European Food Safety Authority. Based on the above results, <i>E. faecium</i> 129 BIO 3B and <i>E. faecium</i> 129 BIO 3B-R were classified as <i>E. lactis</i>. The absence of pathogenic genes except for <i>fms21</i> in this study demonstrates that these bacteria are safe for use as probiotics.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"42 3","pages":"180-185"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/82/70/bmfh-42-180.PMC10315194.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10160014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Turmeronols (A and B) from <i>Curcuma longa</i> have anti-inflammatory effects in lipopolysaccharide-stimulated BV-2 microglial cells by reducing NF-κB signaling.","authors":"Ryosuke Saji,&nbsp;Ryusei Uchio,&nbsp;Arisa Fuwa,&nbsp;Chinatsu Okuda-Hanafusa,&nbsp;Kengo Kawasaki,&nbsp;Koutarou Muroyama,&nbsp;Shinji Murosaki,&nbsp;Yoshihiro Yamamoto,&nbsp;Yoshitaka Hirose","doi":"10.12938/bmfh.2022-071","DOIUrl":"https://doi.org/10.12938/bmfh.2022-071","url":null,"abstract":"Turmeronols (A and B), bisabolane-type sesquiterpenoids found in turmeric, reduce inflammation outside the brain in animals; however, their effects on neuroinflammation, a common pathology of various neurodegenerative diseases, are not understood. Inflammatory mediators produced by microglial cells play a key role in neuroinflammation, so this study evaluated the anti-inflammatory effects of turmeronols in BV-2 microglial cells stimulated with lipopolysaccharide (LPS). Pretreatment with turmeronol A or B significantly inhibited LPS-induced nitric oxide (NO) production; mRNA expression of inducible NO synthase; production of interleukin (IL)-1β, IL-6, and tumor necrosis factor α and upregulation of their mRNA expression; phosphorylation of nuclear factor-κB (NF-κB) p65 proteins and inhibitor of NF-κB kinase (IKK); and nuclear translocation of NF-κB. These results suggest that these turmeronols may prevent the production of inflammatory mediators by inhibiting the IKK/NF-κB signaling pathway in activated microglial cells and can potentially treat neuroinflammation associated with microglial activation.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"42 3","pages":"172-179"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6c/ce/bmfh-42-172.PMC10315188.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10160015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of <i>Bifidobacterium longum</i> BB536 against nausea caused by pirfenidone in a mouse model of pellagra.","authors":"Koji Kuronuma,&nbsp;Natsumi Susai,&nbsp;Tomohiro Kuroita,&nbsp;Takeshi Yoshioka,&nbsp;Atsushi Saito,&nbsp;Hirofumi Chiba","doi":"10.12938/bmfh.2022-042","DOIUrl":"https://doi.org/10.12938/bmfh.2022-042","url":null,"abstract":"<p><p>Pellagra is caused by abnormal intake and/or use of nicotinic acid and is known in part to be induced by the use of medications such as isoniazid or pirfenidone. We previously investigated atypical phenotypes of pellagra, such as nausea, using a mouse model of pellagra and found that gut microbiota play an important role in the development of these phenotypes. Here, we investigated the effect of <i>Bifidobacterium longum</i> BB536 on pellagra-related nausea caused by pirfenidone in our mouse model. Our pharmacological data indicated that pirfenidone (PFD) causes modulation of the gut microbiota profile, which appeared to play an important role in the development of pellagra-related nausea. A gut microbiota-mediated protective effect of <i>B. longum</i> BB536 against nausea caused by PFD was also identified. Finally, the urinary ratio of nicotinamide/N-methylnicotinamide was shown to be a biomarker of pellagra-like adverse effects induced by PFD, and it may contribute to the prevention of these effects in patients with idiopathic pulmonary fibrosis.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"42 3","pages":"195-202"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d3/07/bmfh-42-195.PMC10315189.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9804963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of the most efficacious lactic acid bacteria strain for myocardial infarction recovery and verification and exploration of its functions and mechanisms.","authors":"Shanshan Qi,&nbsp;Donghua Liu,&nbsp;Bo Ma,&nbsp;Lei Yang,&nbsp;Haitao Yu","doi":"10.12938/bmfh.2021-044","DOIUrl":"https://doi.org/10.12938/bmfh.2021-044","url":null,"abstract":"<p><p>Screening efficient strains by cell platform is cost-effective, but to date, no screening experiments have been performed for targeted lactic acid bacteria with hypoxic/reoxygenation (H/R)-treated cardiomyocytes, and their effects on the phosphoinositide 3-kinase (PI3K)/protein kinase b (Akt)/endothelial nitric oxide synthase (eNOS) pathway in myocardial infarction (MI) are unclear. Here we activated 102 strains of lactic acid bacteria and inoculated them into MRS medium for fermentation. The fermentation supernatants of the lactic acid bacteria were incubated with an H/R model of H9C2 cells. We found that <i>Bifidobacterium longum</i> ZL0210 had the greatest potential for inhibiting the apoptosis of H/R-induced H9C2 cells. Furthermore, it significantly increased the expression of heme oxygenase-1 (HO-1) and quinone oxidoreductase 1 (NQO1) in H9C2 cardiomyocytes, as well as the Bcl-2/Bax protein ratio, protecting damaged myocardial cells via an anti-apoptotic pathway. Intragastric administration of <i>B. longum</i> ZL0210 to mice for one week before and after establishment of an MI model drastically attenuated the myocardial cell hypertrophy and fibrosis of the MI mice. Meanwhile, <i>B. longum</i> ZL0210 significantly reduced the secretion of myocardial enzymes, increased the activity of antioxidant enzymes, and inhibited lipid-oxidative malondialdehyde (MDA) levels. Moreover, it upregulated the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein and the phosphorylation levels of PI3K, Akt, and eNOS, resulting in increased NO contents. In summary, we screened 102 strains of lactic acid bacteria with a cell platform and determined that <i>B. longum</i> ZL0210 was a favorable candidate for protecting the myocardium. We are the first to reveal the protective effects of <i>B. longum</i> ZL0210 for MI via activation of the PI3K/Akt/eNOS pathway through TRAIL.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"42 1","pages":"13-23"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c2/fa/bmfh-42-013.PMC9816043.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9103332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}