Screening of the most efficacious lactic acid bacteria strain for myocardial infarction recovery and verification and exploration of its functions and mechanisms.

IF 3.1 4区 医学 Q2 Agricultural and Biological Sciences
Shanshan Qi, Donghua Liu, Bo Ma, Lei Yang, Haitao Yu
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引用次数: 0

Abstract

Screening efficient strains by cell platform is cost-effective, but to date, no screening experiments have been performed for targeted lactic acid bacteria with hypoxic/reoxygenation (H/R)-treated cardiomyocytes, and their effects on the phosphoinositide 3-kinase (PI3K)/protein kinase b (Akt)/endothelial nitric oxide synthase (eNOS) pathway in myocardial infarction (MI) are unclear. Here we activated 102 strains of lactic acid bacteria and inoculated them into MRS medium for fermentation. The fermentation supernatants of the lactic acid bacteria were incubated with an H/R model of H9C2 cells. We found that Bifidobacterium longum ZL0210 had the greatest potential for inhibiting the apoptosis of H/R-induced H9C2 cells. Furthermore, it significantly increased the expression of heme oxygenase-1 (HO-1) and quinone oxidoreductase 1 (NQO1) in H9C2 cardiomyocytes, as well as the Bcl-2/Bax protein ratio, protecting damaged myocardial cells via an anti-apoptotic pathway. Intragastric administration of B. longum ZL0210 to mice for one week before and after establishment of an MI model drastically attenuated the myocardial cell hypertrophy and fibrosis of the MI mice. Meanwhile, B. longum ZL0210 significantly reduced the secretion of myocardial enzymes, increased the activity of antioxidant enzymes, and inhibited lipid-oxidative malondialdehyde (MDA) levels. Moreover, it upregulated the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein and the phosphorylation levels of PI3K, Akt, and eNOS, resulting in increased NO contents. In summary, we screened 102 strains of lactic acid bacteria with a cell platform and determined that B. longum ZL0210 was a favorable candidate for protecting the myocardium. We are the first to reveal the protective effects of B. longum ZL0210 for MI via activation of the PI3K/Akt/eNOS pathway through TRAIL.

筛选对心肌梗死恢复最有效的乳酸菌菌株及其功能机制的验证与探索。
通过细胞平台筛选高效菌株具有成本效益,但迄今为止,尚未对缺氧/再氧化(H/R)处理心肌细胞的靶向乳酸菌进行筛选实验,其对心肌梗死(MI)中磷酸肌肽3激酶(PI3K)/蛋白激酶b (Akt)/内皮型一氧化氮合酶(eNOS)途径的影响尚不清楚。本实验对102株乳酸菌进行了活化,并将其接种于MRS培养基中发酵。将乳酸菌发酵上清液与H9C2细胞H/R模型孵育。我们发现长双歧杆菌ZL0210抑制H/ r诱导的H9C2细胞凋亡的潜力最大。显著提高H9C2心肌细胞血红素加氧酶-1 (HO-1)和醌氧化还原酶1 (NQO1)表达及Bcl-2/Bax蛋白比值,通过抗凋亡途径保护受损心肌细胞。小鼠在心肌梗死模型建立前后灌胃1周,长叶双歧杆菌ZL0210可明显减轻心肌细胞的肥大和纤维化。同时,长叶扁豆ZL0210显著降低心肌酶分泌,提高抗氧化酶活性,抑制脂质氧化丙二醛(MDA)水平。上调肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor-related apoptosis-inducing ligand, TRAIL)蛋白表达及PI3K、Akt、eNOS磷酸化水平,导致NO含量升高。综上所述,我们利用细胞平台筛选了102株乳酸菌,确定长芽孢杆菌ZL0210是保护心肌的有利候选菌株。我们首次揭示了长芽胞杆菌ZL0210通过TRAIL激活PI3K/Akt/eNOS通路对心肌梗死的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bioscience of Microbiota, Food and Health
Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
5.50
自引率
3.20%
发文量
24
期刊介绍: Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.
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