枯草芽孢杆菌TO-A延长秀丽隐杆线虫的寿命。

IF 3.1 4区 医学 Q2 Agricultural and Biological Sciences
Ryuichi Saito, Naoki Sato, Yoichi Okino, Dian-Sheng Wang, Genichiro Seo
{"title":"枯草芽孢杆菌TO-A延长秀丽隐杆线虫的寿命。","authors":"Ryuichi Saito,&nbsp;Naoki Sato,&nbsp;Yoichi Okino,&nbsp;Dian-Sheng Wang,&nbsp;Genichiro Seo","doi":"10.12938/bmfh.2022-057","DOIUrl":null,"url":null,"abstract":"<p><p><i>Clostridium butyricum</i> TO-A, <i>Enterococcus faecium</i> T-110, and <i>Bacillus subtilis</i> TO-A are sold as oral probiotic preparations and reportedly exhibit many beneficial effects on the health of hosts, including humans and livestock. In this study, we compared the ability of these clinically applied probiotic bacteria with <i>Escherichia coli</i> OP50 in extending the lifespan of <i>Caenorhabditis elegans</i>. To compare the <i>C. elegans</i> lifespan-extending effects of the three bacteria, experiments were performed using a nematode growth medium containing a small amount of trypticase soy agar. The maximum lifespans of worms fed <i>C. butyricum</i> TO-A, <i>E. faecium</i> T-110, or <i>B. subtilis</i> TO-A increased by 11, 12, and 26%, respectively, compared with worms fed <i>E. coli</i> OP50. In addition, we conducted a metabolomic analysis of methanol extracts of <i>B. subtilis</i> TO-A cells, which exhibited the strongest lifespan-extending effect on <i>C. elegans</i> among the probiotic bacteria tested in this study. As a result, 59 candidate substances involved in extending the lifespan of <i>C. elegans</i> were identified in <i>B. subtilis</i> TO-A cells.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/e9/bmfh-42-124.PMC10067327.pdf","citationCount":"1","resultStr":"{\"title\":\"<i>Bacillus subtilis</i> TO-A extends the lifespan of <i>Caenorhabditis elegans</i>.\",\"authors\":\"Ryuichi Saito,&nbsp;Naoki Sato,&nbsp;Yoichi Okino,&nbsp;Dian-Sheng Wang,&nbsp;Genichiro Seo\",\"doi\":\"10.12938/bmfh.2022-057\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Clostridium butyricum</i> TO-A, <i>Enterococcus faecium</i> T-110, and <i>Bacillus subtilis</i> TO-A are sold as oral probiotic preparations and reportedly exhibit many beneficial effects on the health of hosts, including humans and livestock. In this study, we compared the ability of these clinically applied probiotic bacteria with <i>Escherichia coli</i> OP50 in extending the lifespan of <i>Caenorhabditis elegans</i>. To compare the <i>C. elegans</i> lifespan-extending effects of the three bacteria, experiments were performed using a nematode growth medium containing a small amount of trypticase soy agar. The maximum lifespans of worms fed <i>C. butyricum</i> TO-A, <i>E. faecium</i> T-110, or <i>B. subtilis</i> TO-A increased by 11, 12, and 26%, respectively, compared with worms fed <i>E. coli</i> OP50. In addition, we conducted a metabolomic analysis of methanol extracts of <i>B. subtilis</i> TO-A cells, which exhibited the strongest lifespan-extending effect on <i>C. elegans</i> among the probiotic bacteria tested in this study. As a result, 59 candidate substances involved in extending the lifespan of <i>C. elegans</i> were identified in <i>B. subtilis</i> TO-A cells.</p>\",\"PeriodicalId\":8867,\"journal\":{\"name\":\"Bioscience of Microbiota, Food and Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/e9/bmfh-42-124.PMC10067327.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience of Microbiota, Food and Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.12938/bmfh.2022-057\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience of Microbiota, Food and Health","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.12938/bmfh.2022-057","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1

摘要

丁酸梭菌TO-A、屎肠球菌T-110和枯草芽孢杆菌TO-A作为口服益生菌制剂出售,据报道,它们对宿主(包括人类和牲畜)的健康有许多有益的影响。在这项研究中,我们比较了这些临床应用的益生菌与大肠杆菌OP50在延长秀丽隐杆线虫寿命方面的能力。为了比较这三种细菌对秀丽隐杆线虫的寿命延长效果,实验使用含有少量胰蛋白酶大豆琼脂的线虫生长培养基进行。与大肠杆菌OP50相比,饲喂丁酸梭菌TO-A、粪肠梭菌T-110和枯草芽孢杆菌TO-A的线虫的最大寿命分别增加了11%、12%和26%。此外,我们对枯草芽孢杆菌TO-A细胞的甲醇提取物进行了代谢组学分析,发现在本研究测试的益生菌中,TO-A细胞对秀丽隐杆线虫的寿命延长作用最强。结果,在枯草芽孢杆菌TO-A细胞中鉴定出59种参与延长秀丽隐杆线虫寿命的候选物质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

<i>Bacillus subtilis</i> TO-A extends the lifespan of <i>Caenorhabditis elegans</i>.

<i>Bacillus subtilis</i> TO-A extends the lifespan of <i>Caenorhabditis elegans</i>.

<i>Bacillus subtilis</i> TO-A extends the lifespan of <i>Caenorhabditis elegans</i>.

Bacillus subtilis TO-A extends the lifespan of Caenorhabditis elegans.

Clostridium butyricum TO-A, Enterococcus faecium T-110, and Bacillus subtilis TO-A are sold as oral probiotic preparations and reportedly exhibit many beneficial effects on the health of hosts, including humans and livestock. In this study, we compared the ability of these clinically applied probiotic bacteria with Escherichia coli OP50 in extending the lifespan of Caenorhabditis elegans. To compare the C. elegans lifespan-extending effects of the three bacteria, experiments were performed using a nematode growth medium containing a small amount of trypticase soy agar. The maximum lifespans of worms fed C. butyricum TO-A, E. faecium T-110, or B. subtilis TO-A increased by 11, 12, and 26%, respectively, compared with worms fed E. coli OP50. In addition, we conducted a metabolomic analysis of methanol extracts of B. subtilis TO-A cells, which exhibited the strongest lifespan-extending effect on C. elegans among the probiotic bacteria tested in this study. As a result, 59 candidate substances involved in extending the lifespan of C. elegans were identified in B. subtilis TO-A cells.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Bioscience of Microbiota, Food and Health
Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
5.50
自引率
3.20%
发文量
24
期刊介绍: Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信