Biomicrofluidics最新文献_第5页

Universal correlation for the critical diameter of deterministic lateral displacement devices with polygonal posts 带有多边形支柱的确定性侧向位移装置临界直径的通用相关性

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-07-30 DOI: 10.1063/5.0214178

Sourabh Das, Ishaan Gupta, Supreet Singh Bahga

{"title":"Universal correlation for the critical diameter of deterministic lateral displacement devices with polygonal posts","authors":"Sourabh Das, Ishaan Gupta, Supreet Singh Bahga","doi":"10.1063/5.0214178","DOIUrl":"https://doi.org/10.1063/5.0214178","url":null,"abstract":"Deterministic lateral displacement (DLD) is a microfluidic technique that utilizes a specific array of micro-posts to separate cells or particles larger and smaller than a critical diameter. The critical diameter depends on the shape of the posts, the gap between the posts, and the relative shift between the adjacent rows of posts. Here, we present an experimental and numerical investigation to elucidate the functional dependence of the critical diameter of DLD arrays with polygonal posts on the geometric parameters. Based on simulations of fluid flow through DLD devices with varying geometric parameters, we first derived a correlation to predict the critical diameter of DLD arrays with polygonal post shapes having an arbitrary number of sides. We then used a novel experimental approach, wherein we coupled different DLD arrays with an upstream droplet generator to flow droplets of varying sizes and estimate the critical diameter. The critical diameter predicted by the correlation based on simulations compares well with our experimental data and with data available in the literature. The universal correlation for a critical diameter presented here can help design and optimize DLD devices with polygonal posts.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"213 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141870005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicle and lipoprotein diagnostics (ExoLP-Dx) with membrane sensor: A robust microfluidic platform to overcome heterogeneity. 带膜传感器的细胞外囊泡和脂蛋白诊断（ExoLP-Dx）：克服异质性的强大微流控平台。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-07-24 eCollection Date: 2024-07-01 DOI: 10.1063/5.0218986

Sonu Kumar, Satyajyoti Senapati, Hsueh-Chia Chang

{"title":"Extracellular vesicle and lipoprotein diagnostics (ExoLP-Dx) with membrane sensor: A robust microfluidic platform to overcome heterogeneity.","authors":"Sonu Kumar, Satyajyoti Senapati, Hsueh-Chia Chang","doi":"10.1063/5.0218986","DOIUrl":"10.1063/5.0218986","url":null,"abstract":"The physiological origins and functions of extracellular vesicles (EVs) and lipoproteins (LPs) propel advancements in precision medicine by offering non-invasive diagnostic and therapeutic prospects for cancers, cardiovascular, and neurodegenerative diseases. However, EV/LP diagnostics (ExoLP-Dx) face considerable challenges. Their intrinsic heterogeneity, spanning biogenesis pathways, surface protein composition, and concentration metrics complicate traditional diagnostic approaches. Commonly used methods such as nanoparticle tracking analysis, enzyme-linked immunosorbent assay, and nuclear magnetic resonance do not provide any information about their proteomic subfractions, including active proteins/enzymes involved in essential pathways/functions. Size constraints limit the efficacy of flow cytometry for small EVs and LPs, while ultracentrifugation isolation is hampered by co-elution with non-target entities. In this perspective, we propose a charge-based electrokinetic membrane sensor, with silica nanoparticle reporters providing salient features, that can overcome the interference, long incubation time, sensitivity, and normalization issues of ExoLP-Dx from raw plasma without needing sample pretreatment/isolation. A universal EV/LP standard curve is obtained despite their heterogeneities.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 4","pages":"041301"},"PeriodicalIF":2.6,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11272220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manipulating the duration of picoinjection controls the injected volume of individual droplets 控制皮米注射的持续时间可控制单个液滴的注射量

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-07-02 DOI: 10.1063/5.0206830

R. Thakur, D. Weitz

引用次数: 0

A method to prevent clogging and clustering in microfluidic systems using microbubble streaming 利用微气泡流防止微流体系统堵塞和聚集的方法

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-07-02 DOI: 10.1063/5.0214436

Amirabas Bakhtiari, Christian J. Kähler

{"title":"A method to prevent clogging and clustering in microfluidic systems using microbubble streaming","authors":"Amirabas Bakhtiari, Christian J. Kähler","doi":"10.1063/5.0214436","DOIUrl":"https://doi.org/10.1063/5.0214436","url":null,"abstract":"This paper presents an innovative strategy to address the issues of clogging and cluster-related challenges in microchannels within microfluidic devices. Leveraging three-dimensional (3D) microbubble streaming as a dynamic solution, our approach involves the controlled activation of microbubbles near channel constrictions, inducing microstreaming with distinctive features. This microstreaming, characterized by a high non-uniform 3D gradient and significant shear stress, effectively inhibits arch formation at constrictions and disintegrates particle clusters, demonstrating real-time prevention of clogging incidents and blockages. This study includes experimental validation of the anti-clogging technique, a detailed examination of microstreaming phenomena, and their effects on clogging and clustering issues. It also incorporates statistical analyses performed in various scenarios to verify the method’s effectiveness and adaptability. Moreover, a versatile control system has been designed that operates in event-triggered, continuous, or periodic modes, which suits different lab-on-a-chip applications and improves the overall functionality of microfluidic systems.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"16 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141524983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel viscoelastic microfluidic platform for nanoparticle/small extracellular vesicle separation through viscosity gradient-induced migration. 通过粘度梯度诱导迁移实现纳米粒子/小细胞外囊泡分离的新型粘弹性微流体平台。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-06-26 eCollection Date: 2024-05-01 DOI: 10.1063/5.0208417

Han Guo, Dayin Wang, Shilun Feng, Kaihuan Zhang, Yuan Luo, Jianlong Zhao

{"title":"A novel viscoelastic microfluidic platform for nanoparticle/small extracellular vesicle separation through viscosity gradient-induced migration.","authors":"Han Guo, Dayin Wang, Shilun Feng, Kaihuan Zhang, Yuan Luo, Jianlong Zhao","doi":"10.1063/5.0208417","DOIUrl":"10.1063/5.0208417","url":null,"abstract":"Small extracellular vesicles (sEVs) are extracellular vesicles with diameters ranging from 30 to 150 nm, harboring proteins and nucleic acids that reflect their source cells and act as vital mediators of intercellular communication. The comprehensive analysis of sEVs is hindered by the complex composition of biofluids that contain various extracellular vesicles. Conventional separation methods, such as ultracentrifugation and immunoaffinity capture, face routine challenges in operation complexity, cost, and compromised recovery rates. Microfluidic technologies, particularly viscoelastic microfluidics, offer a promising alternative for sEV separation due to its field-free nature, fast and simple operation procedure, and minimal sample consumption. In this context, we here introduce an innovative viscoelastic approach designed to exploit the viscosity gradient-induced force with size-dependent characteristics, thereby enabling the efficient separation of nano-sized particles and sEVs from larger impurities. We first seek to illustrate the underlying mechanism of the viscosity gradient-induced force, followed by experimental validation with fluorescent nanoparticles demonstrating separation results consistent with qualitative analysis. We believe that this work is the first to report such viscosity gradient-induced phenomenon in the microfluidic context. The presented approach achieves ∼80% for both target purity and recovery rate. We further demonstrate effective sEV separation using our device to showcase its efficacy in the real biological context, highlighting its potential as a versatile, label-free platform for sEV analysis in both fundamental biological research and clinical applications.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 3","pages":"034107"},"PeriodicalIF":2.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic gut-axis-on-a-chip models for pharmacokinetic-based disease models. 基于药物动力学的疾病模型的微流控芯片肠道轴模型。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-06-26 eCollection Date: 2024-05-01 DOI: 10.1063/5.0206271

Raehyun Kim, Jong Hwan Sung

引用次数: 0

Application and prospect of microfluidic devices for rapid assay of cell activities in the tumor microenvironment. 微流控装置在快速检测肿瘤微环境中细胞活性方面的应用和前景。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-06-17 eCollection Date: 2024-05-01 DOI: 10.1063/5.0206058

Linjing Zhu, Xueling Cui, Lingling Jiang, Fang Fang, Boyang Liu

{"title":"Application and prospect of microfluidic devices for rapid assay of cell activities in the tumor microenvironment.","authors":"Linjing Zhu, Xueling Cui, Lingling Jiang, Fang Fang, Boyang Liu","doi":"10.1063/5.0206058","DOIUrl":"10.1063/5.0206058","url":null,"abstract":"The global impact of cancer on human health has raised significant concern. In this context, the tumor microenvironment (TME) plays a pivotal role in the tumorigenesis and malignant progression. In order to enhance the accuracy and efficacy of therapeutic outcomes, there is an imminent requirement for in vitro models that can accurately replicate the intricate characteristics and constituents of TME. Microfluidic devices exhibit notable advantages in investigating the progression and treatment of tumors and have the potential to become a novel methodology for evaluating immune cell activities in TME and assist clinicians in assessing the prognosis of patients. In addition, it shows great advantages compared to traditional cell experiments. Therefore, the review first outlines the applications and advantages of microfluidic chips in facilitating tumor cell culture, constructing TME and investigating immune cell activities. Second, the roles of microfluidic devices in the analysis of circulating tumor cells, tumor prognosis, and drug screening have also been mentioned. Moreover, a forward-looking perspective is discussed, anticipating the widespread clinical adoption of microfluidic devices in the future.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 3","pages":"031506"},"PeriodicalIF":2.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic technologies for advanced antimicrobial susceptibility testing. 用于高级抗菌药物药敏试验的微流控技术。

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-06-07 eCollection Date: 2024-05-01 DOI: 10.1063/5.0190112

Wenshuai Wu, Ying Mu

引用次数: 0

Recent advances in microfluidic-based spectroscopic approaches for pathogen detection. 基于微流体的病原体检测光谱方法的最新进展。

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-06-07 eCollection Date: 2024-05-01 DOI: 10.1063/5.0204987

Mubashir Hussain, Xu He, Chao Wang, Yichuan Wang, Jingjing Wang, Mingyue Chen, Haiquan Kang, Na Yang, Xinye Ni, Jianqing Li, Xiuping Zhou, Bin Liu

{"title":"Recent advances in microfluidic-based spectroscopic approaches for pathogen detection.","authors":"Mubashir Hussain, Xu He, Chao Wang, Yichuan Wang, Jingjing Wang, Mingyue Chen, Haiquan Kang, Na Yang, Xinye Ni, Jianqing Li, Xiuping Zhou, Bin Liu","doi":"10.1063/5.0204987","DOIUrl":"10.1063/5.0204987","url":null,"abstract":"Rapid identification of pathogens with higher sensitivity and specificity plays a significant role in maintaining public health, environmental monitoring, controlling food quality, and clinical diagnostics. Different methods have been widely used in food testing laboratories, quality control departments in food companies, hospitals, and clinical settings to identify pathogens. Some limitations in current pathogens detection methods are time-consuming, expensive, and laborious sample preparation, making it unsuitable for rapid detection. Microfluidics has emerged as a promising technology for biosensing applications due to its ability to precisely manipulate small volumes of fluids. Microfluidics platforms combined with spectroscopic techniques are capable of developing miniaturized devices that can detect and quantify pathogenic samples. The review focuses on the advancements in microfluidic devices integrated with spectroscopic methods for detecting bacterial microbes over the past five years. The review is based on several spectroscopic techniques, including fluorescence detection, surface-enhanced Raman scattering, and dynamic light scattering methods coupled with microfluidic platforms. The key detection principles of different approaches were discussed and summarized. Finally, the future possible directions and challenges in microfluidic-based spectroscopy for isolating and detecting pathogens using the latest innovations were also discussed.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 3","pages":"031505"},"PeriodicalIF":3.2,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11162289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic sweat patch based on capillary force and evaporation pump for real-time continuous sweat analysis 基于毛细管力和蒸发泵的微流控汗液贴片，用于实时连续汗液分析

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-05-31 DOI: 10.1063/5.0208075

Xiujun Fu, Ye Qiu, Hengjie Zhang, Ye Tian, Aiping Liu, Huaping Wu

{"title":"Microfluidic sweat patch based on capillary force and evaporation pump for real-time continuous sweat analysis","authors":"Xiujun Fu, Ye Qiu, Hengjie Zhang, Ye Tian, Aiping Liu, Huaping Wu","doi":"10.1063/5.0208075","DOIUrl":"https://doi.org/10.1063/5.0208075","url":null,"abstract":"In addition to the common blood and urine, fresh sweat contains a diverse range of physiological indicators that can effectively reflect changes in the body’s state. Wearable sweat sensors are crucial for understanding human physiological health; however, real-time in situ measurement of multiple biomarkers in sweat remains a significant challenge. Here, we propose a wearable microfluidic patch featuring an integrated microfluidic channel and evaporation pump for accelerated and continuous sweat collection, eliminating the need for additional sweat storage cavities that typically impede real-time detection. Capillary forces are harnessed to facilitate the rapid flow of sweat through the detection area, while an evaporation pump based on porous laser-induced graphene enhances sweat evaporation. The synergistic integration of these two components enables an uninterrupted flow of fresh sweat within the patch, ensuring real-time monitoring. The influence of channel size parameters on sweat flow velocity is analyzed, and the optimal width-to-height ratio for achieving the desired flow velocity is determined. By implementing a multi-channel parallel design with chamfering, liquid flow resistance is effectively reduced. Furthermore, the patch integrates sensor modules for sodium ion, chloride ion, glucose, and pH value measurements, ensuring excellent sealing and stability of the assembled system. This work presents a simplified approach to developing wearable sweat sensors that hold the potential for health monitoring and disease diagnosis.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"33 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141191640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0