Biomicrofluidics最新文献_第5页

Dynamic cellular responses to gravitational forces: Exploring the impact on white blood cell(s). 细胞对重力的动态反应：探索对白细胞的影响。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-10-21 eCollection Date: 2024-09-01 DOI: 10.1063/5.0216617

Anirudh Murali, Ram Rup Sarkar

{"title":"Dynamic cellular responses to gravitational forces: Exploring the impact on white blood cell(s).","authors":"Anirudh Murali, Ram Rup Sarkar","doi":"10.1063/5.0216617","DOIUrl":"https://doi.org/10.1063/5.0216617","url":null,"abstract":"In recent years, the allure of space exploration and human spaceflight has surged, yet the effects of microgravity on the human body remain a significant concern. Immune and red blood cells rely on hematic or lymphatic streams as their primary means of transportation, posing notable challenges under microgravity conditions. This study sheds light on the intricate dynamics of cell behavior when suspended in bio-fluid under varying gravitational forces. Utilizing the dissipative particle dynamics approach, blood and white blood cells were modeled, with gravity applied as an external force along the vertical axis, ranging from 0 to 2 g in parameter sweeps. The results revealed discernible alterations in the cell shape and spatial alignment in response to gravity, quantified through metrics such as elongation and deformation indices, pitch angle, and normalized center of mass. Statistical analysis using the Mann-Whitney U test underscored clear distinctions between microgravity (<1 g) and hypergravity (>1 g) samples compared to normal gravity (1 g). Furthermore, the examination of forces exerted on the solid, including drag, shear stress, and solid forces, unveiled a reduction in the magnitude as the gravitational force increased. Additional analysis through dimensionless numbers unveiled the dominance of capillary and gravitational forces, which impacted cell velocity, leading to closer proximity to the wall and heightened viscous interaction with surrounding fluid particles. These interactions prompted shape alterations and reduced white blood cell area while increasing red blood cells. This study represents an effort in comprehending the effects of gravity on blood cells, offering insights into the intricate interplay between cellular dynamics and gravitational forces.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 5","pages":"054112"},"PeriodicalIF":2.6,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11495877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent developments in preventing catheter-related infections based on biofilms: A comprehensive review. 基于生物膜预防导管相关感染的最新进展：全面回顾。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-10-11 eCollection Date: 2024-09-01 DOI: 10.1063/5.0195165

Byeongchan So, Jongwon Kim, Jung Ki Jo, Hongyun So

引用次数: 0

Trajectory analysis of Bacillus subtilis in micro-droplets. 微滴中枯草芽孢杆菌的轨迹分析。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-10-09 eCollection Date: 2024-09-01 DOI: 10.1063/5.0211134

Yangyang Tang, Xiaolei Cao, Rui Kong, Xianyong Li, Jiankun Wang, Jin Wu, Xiaoling Wang

{"title":"Trajectory analysis of Bacillus subtilis in micro-droplets.","authors":"Yangyang Tang, Xiaolei Cao, Rui Kong, Xianyong Li, Jiankun Wang, Jin Wu, Xiaoling Wang","doi":"10.1063/5.0211134","DOIUrl":"https://doi.org/10.1063/5.0211134","url":null,"abstract":"In order to study Bacillus subtilis biofilm formation in microdroplets, we use microfluidics technology to make the droplets and confocal microscopy to capture bacterial movement and biofilm formation in the droplets. We develop a multi-target tracking methodology, using a YOLOv5 detector to identify cells and a DeepSORT algorithm to track cell movements. We find that Bacillus subtilis bacteria with autonomous migration and biofilm-forming ability prefer to cluster and swarm near the microdroplet surface, rather than in the droplet interior. Bacterial mobility depends on phenotype and spatial location within the droplet. The motile cells move about 3.5 times faster than the matrix-producing cells. When the cells are near the wall of the droplet, the direction of the motion of motile cells is along that wall. When the cells are inside the droplet, the direction of the motion of motile cells is disordered, i.e., there is no clear directional or goal-oriented movement. This contrast increases the cell contact probability and facilitates the formation of a Bacillus subtilis biofilm in the droplet. Furthermore, we develop a mathematical model to describe the motion behavior of Bacillus subtilis in microdroplets, which is useful for exploring the influence of motility on biofilm formation.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 5","pages":"054111"},"PeriodicalIF":2.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11466507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple guideline for designing droplet microfluidic chips to achieve an improved single (bio)particle encapsulation rate using a stratified flow-assisted particle ordering method. 设计液滴微流控芯片的简单指南，利用分层流动辅助粒子排序法提高单个（生物）粒子的封装率。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-10-09 eCollection Date: 2024-09-01 DOI: 10.1063/5.0219528

Thu H Nguyen, Noura Ezzo, Sarah Chan, Evelyn K F Yim, Carolyn L Ren

{"title":"A simple guideline for designing droplet microfluidic chips to achieve an improved single (bio)particle encapsulation rate using a stratified flow-assisted particle ordering method.","authors":"Thu H Nguyen, Noura Ezzo, Sarah Chan, Evelyn K F Yim, Carolyn L Ren","doi":"10.1063/5.0219528","DOIUrl":"https://doi.org/10.1063/5.0219528","url":null,"abstract":"Encapsulation of a single (bio)particle into individual droplets (referred to as single encapsulation) presents tremendous potential for precise biological and chemical reactions at the single (bio)particle level. Previously demonstrated successful strategies often rely on the use of high flow rates, gel, or viscoelastic materials for initial cell ordering prior to encapsulation into droplets, which could potentially challenge the system's operation. We propose to enhance the single encapsulation rate by using a stratified flow structure to focus and pre-order the (bio)particles before encapsulation. The stratified flow structure is formed using two simple aqueous Newtonian fluids with a viscosity contrast, which together serve as the dispersed phase. The single encapsulation rate is influenced by many parameters, including fluid viscosity contrast, geometric conditions, flow conditions and flow rate ratios, and dimensionless numbers such as the capillary number. This study focuses on investigating the influences of these parameters on the focused stream of the stratified flow, which is key for single encapsulation. The results allow the proposal of a simple guideline that can be adopted to design droplet microfluidic chips with an improved single encapsulation rate demanded by a wide range of applications. The guideline was validated by performing the single encapsulation of mouse embryonic stem cells suspended in a gelatin-methacryloyl solution in individual droplets of phosphate buffer saline, achieving a single encapsulation efficiency of up to 70%.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 5","pages":"054110"},"PeriodicalIF":2.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11466506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualizing liquid distribution across hyphal networks with cellular resolution. 以细胞分辨率观察液体在整个菌丝网络中的分布。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-10-07 eCollection Date: 2024-09-01 DOI: 10.1063/5.0231656

Amelia J Clark, Emily Masters-Clark, Eleonora Moratto, Pilar Junier, Claire E Stanley

{"title":"Visualizing liquid distribution across hyphal networks with cellular resolution.","authors":"Amelia J Clark, Emily Masters-Clark, Eleonora Moratto, Pilar Junier, Claire E Stanley","doi":"10.1063/5.0231656","DOIUrl":"https://doi.org/10.1063/5.0231656","url":null,"abstract":"Filamentous fungi and fungal-like organisms contribute to a wide range of important ecosystem functions. Evidence has shown the movement of liquid across mycelial networks in unsaturated environments, such as soil. However, tools to investigate liquid movement along hyphae at the level of the single cell are still lacking. Microfluidic devices permit the study of fungal and fungal-like organisms with cellular resolution as they can confine hyphae to a single optical plane, which is compatible with microscopy imaging over longer timescales and allows for precise control of the microchannel environment. The aim of this study was to develop a method that enables the visualization and quantification of liquid movement on hyphae of fungal and fungal-like microorganisms. For this, the fungal-fungal interaction microfluidic device was modified to allow for the maintenance of unsaturated microchannel conditions. Fluorescein-containing growth medium solidified with agar was used to track liquid transported by hyphae via fluorescence microscopy. Our key findings highlight the suitability of this novel methodology for the visualization of liquid movement by hyphae over varying time scales and the ability to quantify the movement of liquid along hyphae. Furthermore, we showed that at the cellular level, extracellular movement of liquid along hyphae can be bidirectional and highly dynamic, uncovering a possible link between liquid movement and hyphal growth characteristics. We envisage that this method can be applied to facilitate future research probing the parameters contributing to hyphal liquid movement and is an essential step for studying the phenomenon of fungal highways.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 5","pages":"054109"},"PeriodicalIF":2.6,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colloidal droplet desiccation on a electrowetting-on-dielectric (EWOD) platform. 电介质电润湿（EWOD）平台上的胶体液滴干燥。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-10-02 eCollection Date: 2024-09-01 DOI: 10.1063/5.0209815

Udita Uday Ghosh, Trina Dhara, Janesh Bakshi, Kalpita Nath, Sunando DasGupta

{"title":"Colloidal droplet desiccation on a electrowetting-on-dielectric (EWOD) platform.","authors":"Udita Uday Ghosh, Trina Dhara, Janesh Bakshi, Kalpita Nath, Sunando DasGupta","doi":"10.1063/5.0209815","DOIUrl":"10.1063/5.0209815","url":null,"abstract":"The physics of the effects of electric field on the desiccation of colloidal droplets, comprising of dispersed negatively charged nanoparticles [2 μl, 1(w/w. %)], are studied in a standard electrowetting-on-a-dielectric configuration. The extent of contact line pinning during evaporation is found to be a function of the magnitude of the applied voltage and quantified in terms of the dimensionless electrowetting number (η). The pinned contact line led to higher particle compaction as evidenced by the characterization of dried colloidal film thicknesses. Crack formation and their dynamics have been analyzed in detail to elicit the interplay of forces near the contact line region and on the compaction front. These aspects of crack formation are elucidated in the light of magnitude and polarity of the applied electric field. It is found to influence the crack front initiation velocity, the geometry, the number of cracks, and an attempt is made to explain the same via first principle-based approaches. Therefore, this study indicates the possibility of using electrowetting as a technique to fine-tune the crack formation behavior in thin colloidal films.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 5","pages":"054108"},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial blood for therapeutic and laboratory usage: Where do we stand? 用于治疗和实验室的人造血液：现状如何？

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-09-25 eCollection Date: 2024-09-01 DOI: 10.1063/5.0186931

Pulak Kumar Ray, Pawan Kumar, Somnath Roy, Arup Kumar Das, Prasanta Kumar Das

{"title":"Artificial blood for therapeutic and laboratory usage: Where do we stand?","authors":"Pulak Kumar Ray, Pawan Kumar, Somnath Roy, Arup Kumar Das, Prasanta Kumar Das","doi":"10.1063/5.0186931","DOIUrl":"https://doi.org/10.1063/5.0186931","url":null,"abstract":"The scarcity of blood for transfusion purposes has been widely acknowledged. Surgical therapeutic processes, war zones, and post-disaster treatments demand a huge amount of blood. Modern-day laboratories also require blood for bioengineering experimentation. Therefore, an artificially devised solution capable of mimicking the blood functions from biological and engineering relevance would be a noteworthy discovery of contemporary science. The experience drawn from discarded century-old blood substitutes has led us to technologically more advanced present-day solutions, which are better at carrying out the physiological functions of blood. Aiming at safety, stability, non-toxicity, and compatibility in terms of immuno-response, a remarkable number of substitutes are being tried to mimic the physiological properties and functions of red blood cells, platelets, plasma, and white blood cells. Despite significant efforts and time devoted, for transfusion, no product so far has been able to replace natural blood. This article puts together the important developments in blood substitutes that have evolved over the years, including substitutes for clinical as well as engineering requirements. It also points out the recent endeavors of synthesizing blood cells through modern synthetic routes. It has been highlighted that none of the blood substitutes have achieved the required efficacy so that they can be used in vivo. Finally, the emerging trends and future research needs have been stressed upon.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 5","pages":"051505"},"PeriodicalIF":2.6,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascularized platforms for investigating cell communication via extracellular vesicles. 通过细胞外囊泡研究细胞通讯的血管化平台。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-09-23 eCollection Date: 2024-09-01 DOI: 10.1063/5.0220840

Junyoung Kim, Jooyoung Ro, Yoon-Kyoung Cho

{"title":"Vascularized platforms for investigating cell communication via extracellular vesicles.","authors":"Junyoung Kim, Jooyoung Ro, Yoon-Kyoung Cho","doi":"10.1063/5.0220840","DOIUrl":"https://doi.org/10.1063/5.0220840","url":null,"abstract":"The vascular network plays an essential role in the maintenance of all organs in the body via the regulated delivery of oxygen and nutrients, as well as tissue communication via the transfer of various biological signaling molecules. It also serves as a route for drug administration and affects pharmacokinetics. Due to this importance, engineers have sought to create physiologically relevant and reproducible vascular systems in tissue, considering cell-cell and extracellular matrix interaction with structural and physical conditions in the microenvironment. Extracellular vesicles (EVs) have recently emerged as important carriers for transferring proteins and genetic material between cells and organs, as well as for drug delivery. Vascularized platforms can be an ideal system for studying interactions between blood vessels and EVs, which are crucial for understanding EV-mediated substance transfer in various biological situations. This review summarizes recent advances in vascularized platforms, standard and microfluidic-based techniques for EV isolation and characterization, and studies of EVs in vascularized platforms. It provides insights into EV-related (patho)physiological regulations and facilitates the development of EV-based therapeutics.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 5","pages":"051504"},"PeriodicalIF":2.6,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11421861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142336268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic supercritical CO 2 applications: Solvent extraction, nanoparticle synthesis, and chemical reaction. 微流控超临界二氧化碳应用：溶剂萃取、纳米粒子合成和化学反应。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-09-23 eCollection Date: 2024-09-01 DOI: 10.1063/5.0215567

Junyi Yang, Peichun Amy Tsai

{"title":"<ArticleTitle xmlns:ns0=\"http://www.w3.org/1998/Math/MathML\">Microfluidic supercritical <ns0:math> <ns0:msub><ns0:mrow><ns0:mi>CO</ns0:mi></ns0:mrow> <ns0:mn>2</ns0:mn></ns0:msub> </ns0:math> applications: Solvent extraction, nanoparticle synthesis, and chemical reaction.","authors":"Junyi Yang, Peichun Amy Tsai","doi":"10.1063/5.0215567","DOIUrl":"https://doi.org/10.1063/5.0215567","url":null,"abstract":"Supercritical <math> <msub><mrow><mi>CO</mi></mrow> <mn>2</mn></msub> </math> , known for its non-toxic, non-flammable and abundant properties, is well-perceived as a green alternative to hazardous organic solvents. It has attracted considerable interest in food, pharmaceuticals, chromatography, and catalysis fields. When supercritical <math> <msub><mrow><mi>CO</mi></mrow> <mn>2</mn></msub> </math> is integrated into microfluidic systems, it offers several advantages compared to conventional macro-scale supercritical reactors. These include optical transparency, small volume, rapid reaction, and precise manipulation of fluids, making microfluidics a versatile tool for process optimization and fundamental studies of extraction and reaction kinetics in supercritical <math> <msub><mrow><mi>CO</mi></mrow> <mn>2</mn></msub> </math> applications. Moreover, the small length scale of microfluidics allows for the production of uniform nanoparticles with reduced particle size, beneficial for nanomaterial synthesis. In this perspective, we review microfluidic investigations involving supercritical <math> <msub><mrow><mi>CO</mi></mrow> <mn>2</mn></msub> </math> , with a particular focus on three primary applications, namely, solvent extraction, nanoparticle synthesis, and chemical reactions. We provide a summary of the experimental innovations, key mechanisms, and principle findings from these microfluidic studies, aiming to spark further interest. Finally, we conclude this review with some discussion on the future perspectives in this field.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 5","pages":"051301"},"PeriodicalIF":2.6,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous high-throughput particle-bacteria separation and solution exchange via in-plane and out-of-plane parallelization of microfluidic centrifuges. 通过微流控离心机的平面内和平面外并行化，同时进行高通量颗粒-细菌分离和溶液交换。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-09-23 eCollection Date: 2024-09-01 DOI: 10.1063/5.0215930

Nima Norouzy, Alireza Zabihihesari, Pouya Rezai

{"title":"Simultaneous high-throughput particle-bacteria separation and solution exchange via in-plane and out-of-plane parallelization of microfluidic centrifuges.","authors":"Nima Norouzy, Alireza Zabihihesari, Pouya Rezai","doi":"10.1063/5.0215930","DOIUrl":"https://doi.org/10.1063/5.0215930","url":null,"abstract":"Inertial microfluidic devices have gained attention for point-of-need (PoN) sample preparation. Yet, devices capable of simultaneous particle-bacteria solution exchange and separation are low in throughput, hindering their applicability to PoN settings. This paper introduces a microfluidic centrifuge for high-throughput solution exchange and separation of microparticles, addressing the need for processing large sample volumes at elevated flow rates. The device integrates Dean flow recirculation and inertial focusing of microparticles within 24 curved microchannels assembled in a three-layer configuration via in-plane and out-of-plane parallelization. We studied solution exchange and particle migration using singleplex and duplex samples across devices with varying curve numbers (2-curve, 8-curve, and 24-curve). Processing 5 and 10 μm microparticles at flow rates up to 16.8 ml/min achieved a solution exchange efficiency of 96.69%. In singleplex solutions, 10 and 5 μm particles selectively migrated to inner and outer outlets, demonstrating separation efficiencies of 99.7% and 90.3%, respectively. With duplex samples, sample purity was measured to be 93.4% and 98.6% for 10 and 5 μm particles collected from the inner and the outer outlets, respectively. Application of our device in biological assays was shown by performing duplex experiments where 10 μm particles were isolated from Salmonella bacterial suspension with purity of 97.8% while increasing the state-of-the-art particle solution exchange and separation throughput by 16 folds. This parallelization enabled desirable combinations of high throughput, low-cost, and scalability, without compromising efficiency and purity, paving the way for sample preparation at the PoN in the future.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 5","pages":"054107"},"PeriodicalIF":2.6,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0