Biomicrofluidics最新文献_第10页

Recent advances in droplet sequential monitoring methods for droplet sorting. 液滴顺序监测方法在液滴分选中的最新进展。

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-11-13 eCollection Date: 2023-12-01 DOI: 10.1063/5.0173340

Yukun He, Yi Qiao, Lu Ding, Tianguang Cheng, Jing Tu

引用次数: 0

Nano/microfluidic device for high-throughput passive trapping of nanoparticles. 用于高通量被动捕获纳米颗粒的纳米/微流体装置。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2023-11-01 eCollection Date: 2023-12-01 DOI: 10.1063/5.0176323

Tanner Wells, Holger Schmidt, Aaron Hawkins

引用次数: 0

Automated design of a 3D passive microfluidic particle sorter. 3D无源微流体颗粒分选机的自动化设计。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2023-11-01 eCollection Date: 2023-12-01 DOI: 10.1063/5.0169562

Kuan-Ming Lai, Zhenya Liu, Yidan Zhang, Junchao Wang, Tsung-Yi Ho

引用次数: 0

Machine learning implementation strategy in imaging and impedance flow cytometry. 成像和阻抗流式细胞术中的机器学习实现策略。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2023-10-27 eCollection Date: 2023-09-01 DOI: 10.1063/5.0166595

Trisna Julian, Tao Tang, Yoichiroh Hosokawa, Yaxiaer Yalikun

{"title":"Machine learning implementation strategy in imaging and impedance flow cytometry.","authors":"Trisna Julian, Tao Tang, Yoichiroh Hosokawa, Yaxiaer Yalikun","doi":"10.1063/5.0166595","DOIUrl":"10.1063/5.0166595","url":null,"abstract":"Imaging and impedance flow cytometry is a label-free technique that has shown promise as a potential replacement for standard flow cytometry. This is due to its ability to provide rich information and archive high-throughput analysis. Recently, significant efforts have been made to leverage machine learning for processing the abundant data generated by those techniques, enabling rapid and accurate analysis. Harnessing the power of machine learning, imaging and impedance flow cytometry has demonstrated its capability to address various complex phenotyping scenarios. Herein, we present a comprehensive overview of the detailed strategies for implementing machine learning in imaging and impedance flow cytometry. We initiate the discussion by outlining the commonly employed setup to acquire the data (i.e., image or signal) from the cell. Subsequently, we delve into the necessary processes for extracting features from the acquired image or signal data. Finally, we discuss how these features can be utilized for cell phenotyping through the application of machine learning algorithms. Furthermore, we discuss the existing challenges and provide insights for future perspectives of intelligent imaging and impedance flow cytometry.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71410438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organoid-on-a-chip: Current challenges, trends, and future scope toward medicine. 芯片上的有机物：当前医学的挑战、趋势和未来范围。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2023-10-27 eCollection Date: 2023-09-01 DOI: 10.1063/5.0171350

Zhangjie Li, Qinyu Li, Chenyang Zhou, Kangyi Lu, Yijun Liu, Lian Xuan, Xiaolin Wang

{"title":"Organoid-on-a-chip: Current challenges, trends, and future scope toward medicine.","authors":"Zhangjie Li, Qinyu Li, Chenyang Zhou, Kangyi Lu, Yijun Liu, Lian Xuan, Xiaolin Wang","doi":"10.1063/5.0171350","DOIUrl":"10.1063/5.0171350","url":null,"abstract":"In vitro organoid models, typically defined as 3D multicellular aggregates, have been extensively used as a promising tool in drug screening, disease progression research, and precision medicine. Combined with advanced microfluidics technique, organoid-on-a-chip can flexibly replicate in vivo organs within the biomimetic physiological microenvironment by accurately regulating different parameters, such as fluid conditions and concentration gradients of biochemical factors. Since engineered organ reconstruction has opened a new paradigm in biomedicine, innovative approaches are increasingly required in micro-nano fabrication, tissue construction, and development of pharmaceutical products. In this Perspective review, the advantages and characteristics of organoid-on-a-chip are first introduced. Challenges in current organoid culture, extracellular matrix building, and device manufacturing techniques are subsequently demonstrated, followed by potential alternative approaches, respectively. The future directions and emerging application scenarios of organoid-on-a-chip are finally prospected to further satisfy the clinical demands.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71410439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on inertial microfluidic fabrication methods. 惯性微流体制备方法综述。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2023-10-19 eCollection Date: 2023-09-01 DOI: 10.1063/5.0163970

Zohreh Akbari, Mohammad Amin Raoufi, Sheyda Mirjalali, Behrouz Aghajanloo

{"title":"A review on inertial microfluidic fabrication methods.","authors":"Zohreh Akbari, Mohammad Amin Raoufi, Sheyda Mirjalali, Behrouz Aghajanloo","doi":"10.1063/5.0163970","DOIUrl":"10.1063/5.0163970","url":null,"abstract":"In recent decades, there has been significant interest in inertial microfluidics due to its high throughput, ease of fabrication, and no need for external forces. The focusing efficiency of inertial microfluidic systems relies entirely on the geometrical features of microchannels because hydrodynamic forces (inertial lift forces and Dean drag forces) are the main driving forces in inertial microfluidic devices. In the past few years, novel microchannel structures have been propounded to improve particle manipulation efficiency. However, the fabrication of these unconventional structures has remained a serious challenge. Although researchers have pushed forward the frontiers of microfabrication technologies, the fabrication techniques employed for inertial microfluidics have not been discussed comprehensively. This review introduces the microfabrication approaches used for creating inertial microchannels, including photolithography, xurography, laser cutting, micromachining, microwire technique, etching, hot embossing, 3D printing, and injection molding. The advantages and disadvantages of these methods have also been discussed. Then, the techniques are reviewed regarding resolution, structures, cost, and materials. This review provides a thorough insight into the manufacturing methods of inertial microchannels, which could be helpful for future studies to improve the harvesting yield and resolution by choosing a proper fabrication technique.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10589053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood component separation in straight microfluidic channels. 直接微流体通道中的血液成分分离。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2023-10-16 eCollection Date: 2023-09-01 DOI: 10.1063/5.0176457

Lap Man Lee, Ketan H Bhatt, Dustin W Haithcock, Balabhaskar Prabhakarpandian

{"title":"Blood component separation in straight microfluidic channels.","authors":"Lap Man Lee, Ketan H Bhatt, Dustin W Haithcock, Balabhaskar Prabhakarpandian","doi":"10.1063/5.0176457","DOIUrl":"10.1063/5.0176457","url":null,"abstract":"Separation of blood components is required in many diagnostic applications and blood processes. In laboratories, blood is usually fractionated by manual operation involving a bulk centrifugation equipment, which significantly increases logistic burden. Blood sample processing in the field and resource-limited settings cannot be readily implemented without the use of microfluidic technology. In this study, we developed a small footprint, rapid, and passive microfluidic channel device that relied on margination and inertial focusing effects for blood component separation. No blood dilution, lysis, or labeling step was needed as to preserve sample integrity. One main innovation of this work was the insertion of fluidic restrictors at outlet ports to divert the separation interface into designated outlet channels. Thus, separation efficiency was significantly improved in comparison to previous works. We demonstrated different operation modes ranging from platelet or plasma extraction from human whole blood to platelet concentration from platelet-rich plasma through the manipulation of outlet port fluidic resistance. Using straight microfluidic channels with a high aspect ratio rectangular cross section, we demonstrated 95.4% platelet purity extracted from human whole blood. In plasma extraction, 99.9% RBC removal rate was achieved. We also demonstrated 2.6× concentration of platelet-rich plasma solution to produce platelet concentrate. The extraction efficiency and throughput rate are scalable with continuous and clog-free recirculation operation, in contrast to other blood fractionation approaches using filtration membranes or affinity-based purification methods. Our microfluidic blood separation method is highly tunable and versatile, and easy to be integrated into multi-step blood processing and advanced sample preparation workflows.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diamond quantum sensors in microfluidics technology. 微流体技术中的金刚石量子传感器。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2023-10-16 eCollection Date: 2023-09-01 DOI: 10.1063/5.0172795

Masazumi Fujiwara

引用次数: 0

All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography. 全聚合物微流控芯片——用于系列晶体学的固定目标样品递送工作台。

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-10-13 eCollection Date: 2023-09-01 DOI: 10.1063/5.0167164

Kevin K Gu, Zhongrui Liu, Sankar Raju Narayanasamy, Megan L Shelby, Nicholas Chan, Matthew A Coleman, Matthias Frank, Tonya L Kuhl

{"title":"All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.","authors":"Kevin K Gu, Zhongrui Liu, Sankar Raju Narayanasamy, Megan L Shelby, Nicholas Chan, Matthew A Coleman, Matthias Frank, Tonya L Kuhl","doi":"10.1063/5.0167164","DOIUrl":"10.1063/5.0167164","url":null,"abstract":"The development of x-ray free electron laser (XFEL) light sources and serial crystallography methodologies has led to a revolution in protein crystallography, enabling the determination of previously unobtainable protein structures and near-atomic resolution of otherwise poorly diffracting protein crystals. However, to utilize XFEL sources efficiently demands the continuous, rapid delivery of a large number of difficult-to-handle microcrystals to the x-ray beam. A recently developed fixed-target system, in which crystals of interest are enclosed within a sample holder, which is rastered through the x-ray beam, is discussed in detail in this Perspective. The fixed target is easy to use, maintains sample hydration, and can be readily modified to allow a broad range of sample types and different beamline requirements. Recent innovations demonstrate the potential of such microfluidic-based fixed targets to be an all-around \"workhorse\" for serial crystallography measurements. This Perspective will summarize recent advancements in microfluidic fixed targets for serial crystallography, examine needs for future development, and guide users in designing, choosing, and utilizing a fixed-target sample delivery device for their system.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576627/pdf/BIOMGB-000017-051302_1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient single-cell oxygen consumption rate characterization based on frequency domain fluorescence lifetime imaging microscopy measurement and microfluidic platform. 基于频域荧光寿命成像显微镜测量和微流体平台的高效单细胞耗氧率表征。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2023-10-13 eCollection Date: 2023-09-01 DOI: 10.1063/5.0161752

Santhosh Kannan, Ping-Liang Ko, Hsiao-Mei Wu, Yi-Chung Tung

{"title":"Efficient single-cell oxygen consumption rate characterization based on frequency domain fluorescence lifetime imaging microscopy measurement and microfluidic platform.","authors":"Santhosh Kannan, Ping-Liang Ko, Hsiao-Mei Wu, Yi-Chung Tung","doi":"10.1063/5.0161752","DOIUrl":"10.1063/5.0161752","url":null,"abstract":"Cell metabolism is critical in regulating normal cell functions to maintain energy homeostasis. In order to monitor cell metabolism, the oxygen consumption rate (OCR) of cells has been characterized as an important factor. In conventional cell analysis, the cells are characterized in bulk due to technical limitations. However, the heterogeneity between the cells cannot be identified. Therefore, single-cell analysis has been proposed to reveal cellular functions and their heterogeneity. In this research, an approach integrating a microfluidic device and widefield frequency domain fluorescence imaging lifetime microscopy (FD-FLIM) for single-cell OCR characterization in an efficient manner is developed. The microfluidic device provides an efficient platform to trap and isolate single cells in microwells with the buffer saline containing an oxygen-sensitive phosphorescent dye. The oxygen tension variation within the microwells can be efficiently estimated by measuring the fluorescence lifetime change using the FD-FLIM, and the OCR values of the single cells can then be calculated. In the experiments, breast cancer (MCF-7) cells are exploited for the OCR measurement. The results demonstrate the functionality of the developed approach and show the heterogeneity among the cells. The developed approach possesses great potential to advance cellular metabolism studies with single-cell resolution.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0