Biomicrofluidics最新文献

Modeling the dynamics of circulating tumor cell clusters inside a microfluidic channel.

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-02-11 eCollection Date: 2025-01-01 DOI: 10.1063/5.0249165

Emmanuel I Ezeobidi, Agnieszka Truszkowska

{"title":"Modeling the dynamics of circulating tumor cell clusters inside a microfluidic channel.","authors":"Emmanuel I Ezeobidi, Agnieszka Truszkowska","doi":"10.1063/5.0249165","DOIUrl":"10.1063/5.0249165","url":null,"abstract":"Circulating tumor cells are central to metastasis, a particularly malign spread of cancer beyond its original location. While rare, there is growing evidence that the clusters of circulating tumor cells are significantly more harmful than individual cells. Microfluidic platforms constitute the core of circulating tumor cell cluster research, allowing cluster detection, analysis, and treatment. In this work, we propose a new mathematical model of circulating tumor cell clusters and apply it to simulate the dynamics of the aggregates inside a microfluidic channel with the external flow of a fluid. We leverage our previous model of the interactions of circulating tumor cells with varying clustering affinities and introduce explicit bonds between the cells that makeup a cluster. We show that the bonds have a visible impact on the cluster dynamics and that they enable the reproduction of known cluster flow and deformation patterns. Furthermore, we demonstrate that the dynamics of these aggregates are sensitive to bond properties, as well as initialization and flow conditions. We believe that our modeling framework represents a valuable mesoscopic formulation with an impact beyond circulating tumor cell clusters, as cell aggregates are common in both nature and applications.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 1","pages":"014103"},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11821273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A microfluidic sucrose gap platform using trilaminar flow with on-chip switching and novel calibration: Challenges and limitations.

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-02-10 eCollection Date: 2025-01-01 DOI: 10.1063/5.0246160

J Dungan, J Mathews, M Levin, V Koomson

{"title":"A microfluidic sucrose gap platform using trilaminar flow with on-chip switching and novel calibration: Challenges and limitations.","authors":"J Dungan, J Mathews, M Levin, V Koomson","doi":"10.1063/5.0246160","DOIUrl":"10.1063/5.0246160","url":null,"abstract":"Gap junction connectivity is crucial to intercellular communication and plays a key role in many critical processes in developmental biology. However, direct analysis of gap junction connectivity in populations of developing cells has proven difficult due to the limitations of patch clamp and dye diffusion based technologies. We re-examine a microfluidic technique based on the principle of laminar flow, which aims to electrically measure gap junction connectivity. In the device, the trilaminar flow of a saline sheathed sucrose solution establishes distinct regions of electrical conductivity in the extracellular fluid spanning an NRK-49F cell monolayer. In theory, the sucrose gap created by laminar flow provides sufficient electrical isolation to detect electrical current flows through the gap junctional network. A novel calibration approach is introduced to account for stream width variation in the device, and elastomeric valves are integrated to improve the performance of gap junction blocker assays. Ultimately, however, this approach is shown to be ineffective in detecting changes in gap junction impedance due to the gap junction blocker, 2-APB. A number of challenges associated with the technique are identified and analyzed in depth and important improvements are described for future iterations.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 1","pages":"014102"},"PeriodicalIF":2.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Processing and inspection of high-pressure microfluidics systems: A review. 高压微流体系统的加工与检测：综述。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-01-06 eCollection Date: 2025-01-01 DOI: 10.1063/5.0235201

Jiangyi Song, Shaoxin Meng, Jianben Liu, Naichao Chen

{"title":"Processing and inspection of high-pressure microfluidics systems: A review.","authors":"Jiangyi Song, Shaoxin Meng, Jianben Liu, Naichao Chen","doi":"10.1063/5.0235201","DOIUrl":"https://doi.org/10.1063/5.0235201","url":null,"abstract":"In the field of microfluidics, high-pressure microfluidics technology, which utilizes high driving pressure for microfluidic analysis, is an evolving technology. This technology combines microfluidics and pressurization, where the flow of fluid is controlled by means of high-pressure-driven devices greater than 10 MPa. This paper first reviews the existing high-pressure microfluidics systems and describes their components and applications. Then, it summarizes several materials used in the microfabrication of high-pressure microfluidics chips, reviewing their properties, processing methods, and bonding methods. In addition, advanced laser processing techniques for the microfabrication of high-pressure microfluidics chips are described. Last, the paper examines the analytical detection methods employed in high-pressure microfluidics systems, encompassing optical and electrochemical detection methods. The review of analytical detection methods shows the different functions and application scenarios of high-pressure microfluidics systems. In summary, this study provides an efficient and advanced microfluidics system, which can be widely used in chemical engineering, food industry, and environmental engineering under high pressure conditions.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 1","pages":"011501"},"PeriodicalIF":2.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of 3D printed chip to improve sensitivity of platelet adhesion through reinjection: Effect of alcohol consumption on platelet adhesion. 3D打印芯片设计通过回注提高血小板粘附灵敏度：酒精消耗对血小板粘附的影响。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-01-03 eCollection Date: 2025-01-01 DOI: 10.1063/5.0237452

Haebeen Kim, Hae-Ryoun Park, Jae Min Song, Eunseop Yeom

{"title":"Design of 3D printed chip to improve sensitivity of platelet adhesion through reinjection: Effect of alcohol consumption on platelet adhesion.","authors":"Haebeen Kim, Hae-Ryoun Park, Jae Min Song, Eunseop Yeom","doi":"10.1063/5.0237452","DOIUrl":"https://doi.org/10.1063/5.0237452","url":null,"abstract":"Monitoring platelet aggregation is crucial for predicting thrombotic diseases and identifying the risk of bleeding or resistance to antiplatelet drugs. This study developed a microfluidic device to measure platelet activation with high sensitivity. By controlling exposure time through repeated reinjections, the device enables the detection of subtle changes in platelet activity influenced by lifestyle factors, such as alcohol consumption. Using computational fluid dynamics simulations, the design was optimized to achieve moderate shear stresses and fabricated with 3D printing. Experimental results revealed that pillars biased to one side partially accelerate the flow and inhibit platelet adhesion. A distinct difference in platelet adhesion was clearly observed before and after alcohol consumption. Despite the high standard deviations in platelet adhesion area, hematocrit, and viscosity after alcohol consumption, the area covered by adhered platelets increased by 3.12 times compared to that before alcohol consumption. This microfluidic chip offers potential for personalized health monitoring by distinguishing platelet variations caused by lifestyle or dietary habits. However, challenges such as reinjection procedures and large sample volumes require further investigation.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 1","pages":"014101"},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of dcEF on microRNA profiles in glioblastoma and exosomes using a novel microfluidic bioreactor. dcEF对胶质母细胞瘤和外泌体microRNA谱的影响

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-27 eCollection Date: 2024-12-01 DOI: 10.1063/5.0228901

Hsieh-Fu Tsai, Amy Q Shen

{"title":"Impact of dcEF on microRNA profiles in glioblastoma and exosomes using a novel microfluidic bioreactor.","authors":"Hsieh-Fu Tsai, Amy Q Shen","doi":"10.1063/5.0228901","DOIUrl":"10.1063/5.0228901","url":null,"abstract":"Glioblastoma multiforme, the most common type of highly aggressive primary brain tumor, is influenced by complex molecular signaling pathways, where microRNAs (miRNAs) play a critical regulatory role. Originating from glial cells, glioblastoma cells are affected by the physiological direct current electric field (dcEF) in the central nervous system. While dcEF has been shown to affect glioblastoma migration (electrotaxis), the specific impact on glioblastoma intercellular communication and miRNA expression in glioblastoma cells and their exosomes remains unclear. This study aims to fill this gap by investigating the differential expression of microRNAs in glioblastoma cells and exosomes under dcEF stimulation. We have developed a novel, reversibly sealed dcEF stimulation bioreactor that ensures uniform dcEF stimulation across a large cell culture area, specifically targeting glioblastoma cells and primary human astrocytes. Using microarray analysis, we examined differential miRNA profiles in both cellular and exosomal RNAs. Our study identified shared molecular targets and pathways affected by dcEF stimulation. Our findings reveal significant changes in miRNA expression due to dcEF stimulation, with specific miRNAs, such as hsa-miR-4440 being up-regulated and hsa-miR-3201 and hsa-mir-548g being down-regulated. Future research will focus on elucidating the molecular mechanisms of these miRNAs and their potential as diagnostic biomarkers. The developed platform offers high-quality dcEF stimulation and rapid sample recovery, with potential applications in tissue engineering and multi-omics molecular analysis.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"064106"},"PeriodicalIF":2.6,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11686958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-energy-powered microfluidic devices. 生物能源驱动的微流体装置。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-24 eCollection Date: 2024-12-01 DOI: 10.1063/5.0227248

Yuhan Li, Chuangyi Xu, Yifan Liao, Xiao Chen, Jiang Chen, Fan Yang, Mingyuan Gao

{"title":"Bio-energy-powered microfluidic devices.","authors":"Yuhan Li, Chuangyi Xu, Yifan Liao, Xiao Chen, Jiang Chen, Fan Yang, Mingyuan Gao","doi":"10.1063/5.0227248","DOIUrl":"10.1063/5.0227248","url":null,"abstract":"Bio-microfluidic technologies offer promising applications in diagnostics and therapy, yet they face significant technical challenges, particularly in the need for external power sources, which limits their practicality and user-friendliness. Recent advancements have explored innovative methods utilizing body fluids, motion, and heat to power these devices, addressing the power supply issue effectively. Among these, body-motion and body-heat-powered systems stand out for their potential to create self-sustaining, wearable, and implantable devices. In this Perspective, we focus on the principles and applications of hydrovoltaic cells, biofuel cells, and piezoelectric and triboelectric nanogenerators. Recent strides in energy conversion efficiency, coupled with the development of biocompatible and durable materials, are driving innovation in bio-integrated electronics. Integration with bio-microfluidic platforms further enhances the linkage to the human body and the potential of these devices for personalized healthcare applications. Ongoing research into these areas promises to deliver sustainable and user-friendly solutions for continuous monitoring, diagnostics, and therapy, potentially revolutionizing the landscape of healthcare delivery.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"061303"},"PeriodicalIF":2.6,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Titrating chimeric antigen receptors on CAR T cells enabled by a microfluidic-based dosage-controlled intracellular mRNA delivery platform. 基于微流体的剂量控制细胞内mRNA递送平台在CAR - T细胞上滴定嵌合抗原受体。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1063/5.0231595

Yu-Hsi Chen, Mahnoor Mirza, Ruoyu Jiang, Abraham P Lee

引用次数: 0

Recent developments in microfluidic passive separation to enable purification of platelets for transfusion. 用于输血血小板纯化的微流控被动分离技术的最新进展。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-17 eCollection Date: 2024-12-01 DOI: 10.1063/5.0226060

Mai T P Dinh, Mubasher Iqbal, Kumar Abhishek, Fong W Lam, Sergey S Shevkoplyas

{"title":"Recent developments in microfluidic passive separation to enable purification of platelets for transfusion.","authors":"Mai T P Dinh, Mubasher Iqbal, Kumar Abhishek, Fong W Lam, Sergey S Shevkoplyas","doi":"10.1063/5.0226060","DOIUrl":"10.1063/5.0226060","url":null,"abstract":"Platelet transfusion is a lifesaving therapy intended to prevent and treat bleeding. However, in addition to platelets, a typical unit also contains a large volume of supernatant that accumulates multiple pro-inflammatory contaminants, including residual leukocytes, microaggregates, microparticles, antibodies, and cytokines. Infusion of this supernatant is responsible for virtually all adverse reactions to platelet transfusions. Conventional methods for removing residual leukocytes (leukoreduction) and reducing the volume of transfused supernatant (volume reduction) struggle to mitigate these risks holistically. Leukoreduction filters can remove leukocytes and microaggregates but fail to reduce supernatant volume, whereas centrifugation can reduce volume, but it is ineffective against larger contaminants and damages platelets. Additionally, platelet purification based on these methods is often too logistically complex, time-consuming, and labor-intensive to implement routinely. Emerging microfluidic technologies offer promising alternatives through passive separation mechanisms that enable cell separation with minimal damage and drastically reduced instrumentation size and facility requirements. This review examines recent innovations in microfluidic cell separation that can be used for leukoreduction and volume reduction of platelets. It begins by defining the performance requirements that any separation method must meet to successfully replace conventional methods currently used to perform these tasks. Standard performance metrics are described, including leukocyte depletion efficiency, degree of volume reduction, processing throughput, and platelet recovery. Finally, the review outlines the primary challenges that must be overcome to enable simple-to-use, disposable microfluidic devices capable of both reducing the platelet unit volume and removing pro-inflammatory contaminants, while preserving most functional platelets for transfusion.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"061504"},"PeriodicalIF":2.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanically mediated cargo delivery to cells using microfluidic devices. 利用微流体装置将机械介导的货物输送到细胞。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-06 eCollection Date: 2024-12-01 DOI: 10.1063/5.0240667

Zhiyu Mao, Bori Shi, Jinbo Wu, Xinghua Gao

{"title":"Mechanically mediated cargo delivery to cells using microfluidic devices.","authors":"Zhiyu Mao, Bori Shi, Jinbo Wu, Xinghua Gao","doi":"10.1063/5.0240667","DOIUrl":"10.1063/5.0240667","url":null,"abstract":"Drug delivery technologies, which are a crucial area of research in the field of cell biology, aim to actively or passively deliver drugs to target cells to enhance therapeutic efficacy and minimize off-target effects. In recent years, with advances in drug development, particularly, the increasing demand for macromolecular drugs (e.g., proteins and nucleic acids), novel drug delivery technologies and intracellular cargo delivery systems have emerged as promising tools for cell and gene therapy. These systems include various viral- and chemical-mediated methods as well as physical delivery strategies. Physical methods, such as electroporation and microinjection, have shown promise in early studies but have not been widely adopted due to concerns regarding efficiency and cellular viability. Recently, microfluidic technologies have provided new opportunities for cargo delivery by allowing for precise control of fluid dynamic parameters to achieve efficient and safe penetration of cell membranes, as well as for foreign material transport. Microfluidics-based mechanical delivery methods utilize biophysical phenomena, such as cell constriction and fluid shear, and are associated with high throughput and high transfection efficiency. In this review, we summarize the latest advancements in microfluidic mechanical delivery technologies, and we discuss constriction- and fluid shear-induced delivery strategies. Furthermore, we explore the potential application of artificial intelligence in optimizing cargo delivery technologies, aiming to provide theoretical support and practical guidance for the future development of novel cellular drug delivery technologies.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"061302"},"PeriodicalIF":2.6,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Miniaturized electrophoresis: An integrated microfluidic cartridge with functionalized hydrogel-assisted LAMP for sample-to-answer analysis of nucleic acid. 微型电泳：一个集成的微流体盒与功能化的水凝胶辅助LAMP用于样本到答案的核酸分析。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-04 eCollection Date: 2024-12-01 DOI: 10.1063/5.0211812

Natish Kumar, Monika Kumari, Devtulya Chander, Sandeep Dogra, Asha Chaubey, Ravi Kumar Arun

{"title":"Miniaturized electrophoresis: An integrated microfluidic cartridge with functionalized hydrogel-assisted LAMP for sample-to-answer analysis of nucleic acid.","authors":"Natish Kumar, Monika Kumari, Devtulya Chander, Sandeep Dogra, Asha Chaubey, Ravi Kumar Arun","doi":"10.1063/5.0211812","DOIUrl":"10.1063/5.0211812","url":null,"abstract":"Accurate detection of pathogenic nucleic acids is crucial for early diagnosis, effective treatment, and containment of infectious diseases. It facilitates the timely identification of pathogens, aids in monitoring disease outbreaks, and helps prevent the spread of infections within healthcare settings and communities. We developed a multi-layered, paper-based microfluidic and miniaturized electrophoresis system for rapid nucleic acid extraction, separation, amplification, and detection, designed for resource-limited settings. Constructed from acrylic, transparency film, pressure-sensitive adhesion, and Whatman paper using a CO2 laser, the setup simplifies traditional methods and eliminates the need for complex equipment. DNA extraction and purification are achieved using Zweifach-Fung bifurcation and Fahraeus effect principles, with detection via a hydrogel-assisted colorimetric isothermal reverse transcriptase-loop-mediated isothermal amplification technique. The system accurately identified the SARS-CoV-2 N-gene and β-actin human gene, validated by a compact electrophoresis setup. In clinical validation with 12 patient specimens, the system demonstrated a positive predictive agreement of 83.0% and a negative predictive agreement of 100%. The system achieves a limit of detection of 1 copy/μl and can potentially transform nucleic acid detection assays in healthcare settings. This study addresses key challenges in nucleic acid detection, such as ensuring sample quality and quantity, reducing reliance on sophisticated equipment, preventing contamination, simplifying procedures, and providing rapid and accurate diagnostics for emerging pathogens.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"064104"},"PeriodicalIF":2.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11620794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0