Biomicrofluidics最新文献_第2页

Temperature-dependent microfluidic impedance spectroscopy for non-invasive biofluid characterization. 非侵入性生物流体表征的温度相关微流体阻抗谱。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-05-01 DOI: 10.1063/5.0255847

Tom Wade, Sohini Kar-Narayan

{"title":"Temperature-dependent microfluidic impedance spectroscopy for non-invasive biofluid characterization.","authors":"Tom Wade, Sohini Kar-Narayan","doi":"10.1063/5.0255847","DOIUrl":"https://doi.org/10.1063/5.0255847","url":null,"abstract":"Remote health monitoring has the potential to enable individuals to take control of their own health and well-being and to facilitate a transition toward preventative and personalized healthcare. Sweat can be sampled non-invasively and contains a wealth of information about the metabolic state of an individual, making it an excellent candidate for remote health monitoring. An accurate, rapid, and low-cost biofluid characterization technique is required to enable the widespread use of remote health monitoring. We previously introduced microfluidic impedance spectroscopy for the detection of electrolyte concentration in fluids, whereby a novel device architecture, measurement method, and analysis technique were presented for the characterization of cationic species. The purely electrical nature of this measurement technique removes the intermediate steps inherent in common rival technologies such as optical and electrochemical sensing, offering a range of advantages. In this work, we investigate the effect of temperature on microfluidic impedance spectroscopy of ionic species commonly present in biofluids. We find that the impedance spectra and concentration determination are temperature-dependent; remote health monitoring devices must be calibrated appropriately as they are likely to experience temperature fluctuations. Importantly, we demonstrate the ability of the method to measure the concentration of anionic species alongside that of cationic species, enabling the detection of chloride and lactate, which are useful biomarkers for hydration, cystic fibrosis, fatigue, sepsis, and hypoperfusion. We show that the presence of neutral species does not impair accurate determination of ionic concentration, thus, demonstrating the suitability of microfluidic impedance spectroscopy for non-invasive biofluid characterization.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 3","pages":"034101"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced microfluidic systems with temperature modulation for biological applications. 用于生物应用的具有温度调制的先进微流体系统。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-05-01 DOI: 10.1063/5.0251893

J Ko, J Lee

{"title":"Advanced microfluidic systems with temperature modulation for biological applications.","authors":"J Ko, J Lee","doi":"10.1063/5.0251893","DOIUrl":"https://doi.org/10.1063/5.0251893","url":null,"abstract":"Recent advances in microfluidic technology have shown the importance of precise temperature control in a wide range of biological applications. This perspective review presents a comprehensive overview of state-of-the-art microfluidic platforms that utilize thermal modulation for various applications, such as rapid nucleic acid amplification, targeted hyperthermia for cancer therapy, and efficient cellular lysis. We detail various heating mechanisms-including nanoparticle-driven induction, photothermal conversion, and electrothermal approaches (both external and on-chip)-and discuss how they are integrated within lab-on-a-chip systems. In parallel, advanced multi-modal sensing methods within microfluidics, ranging from conventional integrated sensors to cutting-edge quantum-based techniques using nanodiamond nitrogen-vacancy centers and suspended microchannel resonators, are highlighted. By integrating advanced multi-modal sensing capabilities into these microfluidic platforms, a broader range of applications are enabled, including single-cell analysis, metabolic profiling, and scalable diagnostics. Looking ahead, overcoming challenges in system integration, scalability, and cost-effectiveness will be essential to harnessing their full potential. Future developments in this field are expected to drive the evolution of lab-on-a-chip technologies, ultimately enabling breakthroughs in precision medicine and high-throughput biomedical applications.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 3","pages":"031301"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved acoustic holograms using simulated annealing. 利用模拟退火技术改进声全息图。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-04-15 eCollection Date: 2025-03-01 DOI: 10.1063/5.0258632

Gagana Weerasinghe, Bram Servais, Daniel Heath, Samuel T Martin, David J Collins

引用次数: 0

Mechanical interaction between a hydrogel and an embedded cell in biomicrofluidic applications. 生物微流控应用中水凝胶与嵌入细胞之间的机械相互作用。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-04-04 eCollection Date: 2025-03-01 DOI: 10.1063/5.0263344

Lei Li, Jiaqi Zhang, Pengtao Yue, James J Feng

{"title":"Mechanical interaction between a hydrogel and an embedded cell in biomicrofluidic applications.","authors":"Lei Li, Jiaqi Zhang, Pengtao Yue, James J Feng","doi":"10.1063/5.0263344","DOIUrl":"10.1063/5.0263344","url":null,"abstract":"Thanks to their softness, biocompatibility, porosity, and ready availability, hydrogels are commonly used in microfluidic assays and organ-on-chip devices as a matrix for cells. They not only provide a supporting scaffold for the differentiating cells and the developing organoids, but also serve as the medium for transmitting oxygen, nutrients, various chemical factors, and mechanical stimuli to the cells. From a bioengineering viewpoint, the transmission of forces from fluid perfusion to the cells through the hydrogel is critical to the proper function and development of the cell colony. In this paper, we develop a poroelastic model to represent the fluid flow through a hydrogel containing a biological cell modeled as a hyperelastic inclusion. In geometries representing shear and normal flows that occur frequently in microfluidic experiments, we use finite-element simulations to examine how the perfusion engenders interstitial flow in the gel and displaces and deforms the embedded cell. The results show that pressure is the most important stress component in moving and deforming the cell, and the model predicts the velocity in the gel and stress transmitted to the cell that is comparable to in vitro and in vivo data. This work provides a computational tool to design the geometry and flow conditions to achieve optimal flow and stress fields inside the hydrogels and around the cell.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 2","pages":"024104"},"PeriodicalIF":2.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design automation for deterministic lateral displacement by leveraging deep Q-network. 利用深度q -网络设计确定性横向位移的自动化。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-03-31 eCollection Date: 2025-03-01 DOI: 10.1063/5.0243605

Yuwei Chen, Yidan Zhang, Junchao Wang

{"title":"Design automation for deterministic lateral displacement by leveraging deep Q-network.","authors":"Yuwei Chen, Yidan Zhang, Junchao Wang","doi":"10.1063/5.0243605","DOIUrl":"10.1063/5.0243605","url":null,"abstract":"Despite the widespread application of microfluidic chips in research fields, such as cell biology, molecular biology, chemistry, and life sciences, the process of designing new chips for specific applications remains complex and time-consuming, often relying on experts. To accelerate the development of high-performance and high-throughput microfluidic chips, this paper proposes an automated Deterministic Lateral Displacement (DLD) chip design algorithm based on reinforcement learning. The design algorithm proposed in this paper treats the throughput and sorting efficiency of DLD chips as key optimization objectives, achieving multi-objective optimization. The algorithm integrates existing research results from our team, enabling rapid evaluation and scoring of DLD chip design parameters. Using this comprehensive performance evaluation system and deep Q-network technology, our algorithm can balance optimal separation efficiency and high throughput in the automated design process of DLD chips. Additionally, the quick execution capability of this algorithm effectively guides engineers in developing high-performance and high-throughput chips during the design phase.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 2","pages":"024103"},"PeriodicalIF":2.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung-on-a-chip: From design principles to disease applications. 片上肺：从设计原则到疾病应用。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-03-28 eCollection Date: 2025-03-01 DOI: 10.1063/5.0257908

Yan Qiu, Guoqing Hu

引用次数: 0

Microfluidic tools to model, monitor, and modulate the gut-brain axis. 微流控工具建模，监测和调节肠脑轴。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-03-07 eCollection Date: 2025-03-01 DOI: 10.1063/5.0253041

Hyehyun Kim, Gregory Girardi, Allison Pickle, Testaverde S Kim, Erkin Seker

{"title":"Microfluidic tools to model, monitor, and modulate the gut-brain axis.","authors":"Hyehyun Kim, Gregory Girardi, Allison Pickle, Testaverde S Kim, Erkin Seker","doi":"10.1063/5.0253041","DOIUrl":"10.1063/5.0253041","url":null,"abstract":"The gut-brain axis (GBA) connects the gastrointestinal tract and the central nervous system (CNS) via the peripheral nervous system and humoral (e.g., circulatory and lymphatic system) routes. The GBA comprises a sophisticated interaction between various mammalian cells, gut microbiota, and systemic factors. This interaction shapes homeostatic and pathophysiological processes and plays an important role in the etiology of many disorders including neuropsychiatric conditions. However, studying the underlying processes of GBA in vivo, where numerous confounding factors exist, is challenging. Furthermore, conventional in vitro models fall short of capturing the GBA anatomy and physiology. Microfluidic platforms with integrated sensors and actuators are uniquely positioned to enhance in vitro models by representing the anatomical layout of cells and allowing to monitor and modulate the biological processes with high spatiotemporal resolution. Here, we first briefly describe microfluidic technologies and their utility in modeling the CNS, vagus nerve, gut epithelial barrier, blood-brain barrier, and their interactions. We then discuss the challenges and opportunities for each model, including the use of induced pluripotent stem cells and incorporation of sensors and actuator modalities to enhance the capabilities of these models. We conclude by envisioning research directions that can help in making the microfluidics-based GBA models better-suited to provide mechanistic insight into pathophysiological processes and screening therapeutics.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 2","pages":"021301"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conditions for a microfluidic creep experiment for microparticles using a cross-slot extensional flow device. 跨槽拉伸流装置微流体蠕变实验条件研究。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-03-06 eCollection Date: 2025-03-01 DOI: 10.1063/5.0239475

Sara Ghanbarpour Mamaghani, Joanna B Dahl

{"title":"Conditions for a microfluidic creep experiment for microparticles using a cross-slot extensional flow device.","authors":"Sara Ghanbarpour Mamaghani, Joanna B Dahl","doi":"10.1063/5.0239475","DOIUrl":"10.1063/5.0239475","url":null,"abstract":"The micromechanical measurement field has struggled to establish repeatable techniques because the deforming stresses can be difficult to model. A recent numerical study [Lu et al., J. Fluid Mech. 962, A26 (2023)] showed that viscoelastic capsules flowing through a cross-slot can achieve a quasi-steady strain near the extensional flow stagnation point that is equal to the equilibrium static strain, thereby implying that the capsule's elastic behavior can be captured in continuous device operation. However, no experimental microfluidic cross-slot studies have reported quasi-steady strains for suspended cells or particles to our knowledge. Here, we demonstrate experimentally the conditions necessary for the cross-slot microfluidic device to replicate a uniaxial creep test at the microscale and at relatively high throughput. By using large dimension cross-slots relative to the microparticle diameter, our cross-slot implementation creates an extensional flow region that is large enough for agarose hydrogel microparticles to achieve a strain plateau while dwelling near the stagnation point. This strain plateau will be key for accurately and precisely measuring viscoelastic properties of small microscale biological objects. We propose an analytical mechanical model to extract linear viscoelastic mechanical properties from observed particle strain histories. Particle image velocimetry measurements of the unperturbed velocity field is used to estimate where in the device particles experienced extensional flow and where the mechanical model might be applied to extract mechanical property measurements. Finally, we provide recommendations for applying the cross-slot microscale creep experiment to other biomaterials and criteria to identify particles that likely achieved a quasi-steady strain state.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 2","pages":"024102"},"PeriodicalIF":2.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11888784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts. 从专业化到广泛采用：液滴微流体创新的主要趋势，增强了非专家的可及性。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-03-03 eCollection Date: 2025-03-01 DOI: 10.1063/5.0242599

Jolien Breukers, Karen Ven, Wannes Verbist, Iene Rutten, Jeroen Lammertyn

{"title":"From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts.","authors":"Jolien Breukers, Karen Ven, Wannes Verbist, Iene Rutten, Jeroen Lammertyn","doi":"10.1063/5.0242599","DOIUrl":"10.1063/5.0242599","url":null,"abstract":"Droplet microfluidics has emerged as a versatile and powerful tool for various analytical applications, including single-cell studies, synthetic biology, directed evolution, and diagnostics. Initially, access to droplet microfluidics was predominantly limited to specialized technology labs. However, the landscape is shifting with the increasing availability of commercialized droplet manipulation technologies, thereby expanding its use to non-specialized labs. Although these commercial solutions offer robust platforms, their adaptability is often constrained compared to in-house developed devices. Consequently, both within the industry and academia, significant efforts are being made to further enhance the robustness and automation of droplet-based platforms, not only to facilitate technology transfer to non-expert laboratories but also to reduce experimental failures. This Perspective article provides an overview of recent advancements aimed at increasing the robustness and accessibility of systems enabling complex droplet manipulations. The discussion encompasses diverse aspects such as droplet generation, reagent addition, splitting, washing, incubation, sorting, and dispensing. Moreover, alternative techniques like double emulsions and hydrogel capsules, minimizing or eliminating the need for microfluidic operations by the end user, are explored. These developments are foreseen to facilitate the integration of intricate droplet manipulations by non-expert users in their workflows, thereby fostering broader and faster adoption across scientific domains.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 2","pages":"021302"},"PeriodicalIF":2.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges. 大气冰核粒子生物分析的微流体：展望与挑战。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2025-02-27 eCollection Date: 2025-01-01 DOI: 10.1063/5.0236911

Mark D Tarn, Kirsty J Shaw, Polly B Foster, Jon S West, Ian D Johnston, Daniel K McCluskey, Sally A Peyman, Benjamin J Murray

{"title":"Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges.","authors":"Mark D Tarn, Kirsty J Shaw, Polly B Foster, Jon S West, Ian D Johnston, Daniel K McCluskey, Sally A Peyman, Benjamin J Murray","doi":"10.1063/5.0236911","DOIUrl":"10.1063/5.0236911","url":null,"abstract":"Atmospheric ice-nucleating particles (INPs) make up a vanishingly small proportion of atmospheric aerosol but are key to triggering the freezing of supercooled liquid water droplets, altering the lifetime and radiative properties of clouds and having a substantial impact on weather and climate. However, INPs are notoriously difficult to model due to a lack of information on their global sources, sinks, concentrations, and activity, necessitating the development of new instrumentation for quantifying and characterizing INPs in a rapid and automated manner. Microfluidic technology has been increasingly adopted by ice nucleation research groups in recent years as a means of performing droplet freezing analysis of INPs, enabling the measurement of hundreds or thousands of droplets per experiment at temperatures down to the homogeneous freezing of water. The potential for microfluidics extends far beyond this, with an entire toolbox of bioanalytical separation and detection techniques developed over 30 years for medical applications. Such methods could easily be adapted to biological and biogenic INP analysis to revolutionize the field, for example, in the identification and quantification of ice-nucleating bacteria and fungi. Combined with miniaturized sampling techniques, we can envisage the development and deployment of microfluidic sample-to-answer platforms for automated, user-friendly sampling and analysis of biological INPs in the field that would enable a greater understanding of their global and seasonal activity. Here, we review the various components that such a platform would incorporate to highlight the feasibility, and the challenges, of such an endeavor, from sampling and droplet freezing assays to separations and bioanalysis.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"19 1","pages":"011502"},"PeriodicalIF":2.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11878220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0