Advanced microfluidic systems with temperature modulation for biological applications.

IF 2.6 4区工程技术 Q2 BIOCHEMICAL RESEARCH METHODS

Biomicrofluidics Pub Date : 2025-05-01 DOI:10.1063/5.0251893

J Ko, J Lee

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引用次数: 0

Abstract

Recent advances in microfluidic technology have shown the importance of precise temperature control in a wide range of biological applications. This perspective review presents a comprehensive overview of state-of-the-art microfluidic platforms that utilize thermal modulation for various applications, such as rapid nucleic acid amplification, targeted hyperthermia for cancer therapy, and efficient cellular lysis. We detail various heating mechanisms-including nanoparticle-driven induction, photothermal conversion, and electrothermal approaches (both external and on-chip)-and discuss how they are integrated within lab-on-a-chip systems. In parallel, advanced multi-modal sensing methods within microfluidics, ranging from conventional integrated sensors to cutting-edge quantum-based techniques using nanodiamond nitrogen-vacancy centers and suspended microchannel resonators, are highlighted. By integrating advanced multi-modal sensing capabilities into these microfluidic platforms, a broader range of applications are enabled, including single-cell analysis, metabolic profiling, and scalable diagnostics. Looking ahead, overcoming challenges in system integration, scalability, and cost-effectiveness will be essential to harnessing their full potential. Future developments in this field are expected to drive the evolution of lab-on-a-chip technologies, ultimately enabling breakthroughs in precision medicine and high-throughput biomedical applications.

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用于生物应用的具有温度调制的先进微流体系统。

微流控技术的最新进展显示了精确温度控制在广泛的生物应用中的重要性。这一观点综述了最先进的微流控平台的全面概述，该平台利用热调节进行各种应用，如快速核酸扩增，靶向热疗癌症治疗和有效的细胞裂解。我们详细介绍了各种加热机制，包括纳米颗粒驱动的感应、光热转换和电热方法（包括外部和芯片上），并讨论了如何将它们集成到芯片上的实验室系统中。与此同时，微流体中的先进多模态传感方法，从传统的集成传感器到使用纳米金刚石氮空位中心和悬浮微通道谐振器的尖端量子技术，都得到了强调。通过将先进的多模态传感功能集成到这些微流控平台中，可以实现更广泛的应用，包括单细胞分析、代谢分析和可扩展诊断。展望未来，克服系统集成、可扩展性和成本效益方面的挑战将是充分利用其潜力的关键。该领域的未来发展有望推动芯片实验室技术的发展，最终实现精准医疗和高通量生物医学应用的突破。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomicrofluidics 生物-纳米科技

CiteScore

5.80

自引率

3.10%

发文量

审稿时长

1.3 months

期刊介绍： Biomicrofluidics (BMF) is an online-only journal published by AIP Publishing to rapidly disseminate research in fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena. BMF also publishes research in unique microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. BMF offers quick publication, multimedia capability, and worldwide circulation among academic, national, and industrial laboratories. With a primary focus on high-quality original research articles, BMF also organizes special sections that help explain and define specific challenges unique to the interdisciplinary field of biomicrofluidics. Microfluidic and nanofluidic actuation (electrokinetics, acoustofluidics, optofluidics, capillary) Liquid Biopsy (microRNA profiling, circulating tumor cell isolation, exosome isolation, circulating tumor DNA quantification) Cell sorting, manipulation, and transfection (di/electrophoresis, magnetic beads, optical traps, electroporation) Molecular Separation and Concentration (isotachophoresis, concentration polarization, di/electrophoresis, magnetic beads, nanoparticles) Cell culture and analysis(single cell assays, stimuli response, stem cell transfection) Genomic and proteomic analysis (rapid gene sequencing, DNA/protein/carbohydrate arrays) Biosensors (immuno-assay, nucleic acid fluorescent assay, colorimetric assay, enzyme amplification, plasmonic and Raman nano-reporter, molecular beacon, FRET, aptamer, nanopore, optical fibers) Biophysical transport and characterization (DNA, single protein, ion channel and membrane dynamics, cell motility and communication mechanisms, electrophysiology, patch clamping). Etc...