Biomicrofluidics最新文献_第9页

Acoustophoresis of monodisperse oil droplets in water: Effect of symmetry breaking and non-resonance operation on oil trapping behavior 水中单分散油滴的声震：对称性破坏和非共振操作对油捕集行为的影响

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-12-27 DOI: 10.1063/5.0175400

H. Bazyar, M. H. Kandemir, J. Peper, M. A. B. Andrade, A. L. Bernassau, K. Schroën, R. G. H. Lammertink

{"title":"Acoustophoresis of monodisperse oil droplets in water: Effect of symmetry breaking and non-resonance operation on oil trapping behavior","authors":"H. Bazyar, M. H. Kandemir, J. Peper, M. A. B. Andrade, A. L. Bernassau, K. Schroën, R. G. H. Lammertink","doi":"10.1063/5.0175400","DOIUrl":"https://doi.org/10.1063/5.0175400","url":null,"abstract":"Acoustic manipulation of particles in microchannels has recently gained much attention. Ultrasonic standing wave (USW) separation of oil droplets or particles is an established technology for microscale applications. Acoustofluidic devices are normally operated at optimized conditions, namely, resonant frequency, to minimize power consumption. It has been recently shown that symmetry breaking is needed to obtain efficient conditions for acoustic particle trapping. In this work, we study the acoustophoretic behavior of monodisperse oil droplets (silicone oil and hexadecane) in water in the microfluidic chip operating at a non-resonant frequency and an off-center placement of the transducer. Finite element-based computer simulations are further performed to investigate the influence of these conditions on the acoustic pressure distribution and oil trapping behavior. Via investigating the Gor’kov potential, we obtained an overlap between the trapping patterns obtained in experiments and simulations. We demonstrate that an off-center placement of the transducer and driving the transducer at a non-resonant frequency can still lead to predictable behavior of particles in acoustofluidics. This is relevant to applications in which the theoretical resonant frequency cannot be achieved, e.g., manipulation of biological matter within living tissues.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139056422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micromixing within microfluidic devices: Fundamentals, design, and fabrication 微流体设备中的微混合：基础、设计和制造

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-12-12 DOI: 10.1063/5.0178396

Shuxiang Cai, Yawen Jin, Yun Lin, Yingzheng He, Peifan Zhang, Zhixing Ge, Wenguang Yang

引用次数: 0

Synchronous oscillatory electro-inertial focusing of microparticles 微颗粒的同步振荡电惯性聚焦

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-12-12 DOI: 10.1063/5.0162368

Giridar Vishwanathan, Gabriel Juarez

{"title":"Synchronous oscillatory electro-inertial focusing of microparticles","authors":"Giridar Vishwanathan, Gabriel Juarez","doi":"10.1063/5.0162368","DOIUrl":"https://doi.org/10.1063/5.0162368","url":null,"abstract":"Here, results are presented on the focusing of 1μm polystyrene particle suspensions using a synchronous oscillatory pressure-driven flow and oscillatory electric field in a microfluidic device. The effect of the phase difference between the oscillatory fields on the focusing position and focusing efficiency was investigated. The focusing position of negatively charged polystyrene particles could be tuned anywhere between the channel centerline to the channel walls. Similarly, the focusing efficiency could range from 20% up to 90%, depending on the phase difference, for particle Reynolds numbers of order O(10−4). The migration velocity profile was measured and the peak velocity was found to scale linearly with both the oscillatory pressure-driven flow amplitude and the oscillatory electric field amplitude. Furthermore, the average migration velocity was observed to scale with the cosine of the phase difference between the fields, indicating the coupled non-linear nature of the phenomenon. Last, the peak migration velocity was measured for different particle radii and found to have an inverse relation, where the velocity increased with decreasing particle radius for identical conditions.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138579915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidics-enabled intelligent manufacturing of metal halide perovskite nanocrystals 利用微流体技术智能制造金属卤化物过氧化物纳米晶体

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-12-12 DOI: 10.1063/5.0172135

Xiaobing Tang, Fuqian Yang

引用次数: 0

Toward versatile digital bioanalysis 实现多功能数字生物分析

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-12-07 DOI: 10.1063/5.0174727

Jun Ando, Rikiya Watanabe

引用次数: 0

Vascularized microfluidic models of major organ structures and cancerous tissues 主要器官结构和癌症组织的血管化微流体模型

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-12-06 DOI: 10.1063/5.0159800

Anagha Rama Varma, Parinaz Fathi

引用次数: 0

Effect of the shear rate and residence time on the lysis of AC16 human cardiomyocyte cells via surface acoustic waves 剪切率和停留时间对通过表面声波裂解 AC16 人类心肌细胞的影响

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-12-06 DOI: 10.1063/5.0158977

G. Almanza, R. M. Trujillo, D. Sanchez-Saldaña, Ø. Rosand, M. Høydal, M. Fernandino, C. A. Dorao

{"title":"Effect of the shear rate and residence time on the lysis of AC16 human cardiomyocyte cells via surface acoustic waves","authors":"G. Almanza, R. M. Trujillo, D. Sanchez-Saldaña, Ø. Rosand, M. Høydal, M. Fernandino, C. A. Dorao","doi":"10.1063/5.0158977","DOIUrl":"https://doi.org/10.1063/5.0158977","url":null,"abstract":"The efficient breakage of one cell or a concentration of cells for releasing intracellular material such as DNA, without damaging it, is the first step for several diagnostics or treatment processes. As the cell membrane is easy to bend but resistant to stretching, the exposure of the cell to a shear rate during a short period of time can be sufficient to damage the membrane and facilitate the extraction of DNA. However, how to induce high shear stresses on cells in small microliter volumes samples has remained an elusive problem. Surface acoustic waves operating at high frequencies can induce acoustic streaming leading to shear rates sufficient to cell lysis. Lysis induced by acoustic streaming in sessile droplets has been investigated in the past from the lysis efficiency point of view. However, the effects of the velocity field and shear rate induced by acoustic streaming on the lysis process remain unexplored. Here, we study the lysis of AC16 human cardiomyocytes in microliter droplets under the effect of the shear rate induced by acoustic streaming. It is identified that for a given shear rate, the extracted DNA is also affected by the actuation period which can be attributed to a cycling process that leads to an accumulation of damage on the cell membrane.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138545310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A method to generate perfusable physiologic-like vascular channels within a liver-on-chip model. 一种在肝脏芯片模型中产生可灌注的生理样血管通道的方法。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2023-12-04 eCollection Date: 2023-12-01 DOI: 10.1063/5.0170606

E Ferrari, E Monti, C Cerutti, R Visone, P Occhetta, L G Griffith, M Rasponi

{"title":"A method to generate perfusable physiologic-like vascular channels within a liver-on-chip model.","authors":"E Ferrari, E Monti, C Cerutti, R Visone, P Occhetta, L G Griffith, M Rasponi","doi":"10.1063/5.0170606","DOIUrl":"10.1063/5.0170606","url":null,"abstract":"The human vasculature is essential in organs and tissues for the transport of nutrients, metabolic waste products, and the maintenance of homeostasis. The integration of vessels in in vitro organs-on-chip may, therefore, improve the similarity to the native organ microenvironment, ensuring proper physiological functions and reducing the gap between experimental research and clinical outcomes. This gap is particularly evident in drug testing and the use of vascularized models may provide more realistic insights into human responses to drugs in the pre-clinical phases of the drug development pipeline. In this context, different vascularized liver models have been developed to recapitulate the architecture of the hepatic sinusoid, exploiting either porous membranes or bioprinting techniques. In this work, we developed a method to generate perfusable vascular channels with a circular cross section within organs-on-chip without any interposing material between the parenchyma and the surrounding environment. Through this technique, vascularized liver sinusoid-on-chip systems with and without the inclusion of the space of Disse were designed and developed. The recapitulation of the Disse layer, therefore, a gap between hepatocytes and endothelial cells physiologically present in the native liver milieu, seems to enhance hepatic functionality (e.g., albumin production) compared to when hepatocytes are in close contact with endothelial cells. These findings pave the way to numerous further uses of microfluidic technologies coupled with vascularized tissue models (e.g., immune system perfusion) as well as the integration within multiorgan-on-chip settings.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10697721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138497732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the synergy of biomicrofluidic technologies and real-time 3D tracking: A perspective. 论生物微流控技术与实时3D跟踪的协同作用:一个视角。

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-12-04 eCollection Date: 2023-12-01 DOI: 10.1063/5.0174269

Liu Hong, Leonardo P Chamorro

引用次数: 0

Gas transport mechanisms through gas-permeable membranes in microfluidics: A perspective. 微流体中气体通过透气膜的输运机制:一个观点。

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2023-11-16 eCollection Date: 2023-12-01 DOI: 10.1063/5.0169555

Sangjin Seo, Taesung Kim

{"title":"Gas transport mechanisms through gas-permeable membranes in microfluidics: A perspective.","authors":"Sangjin Seo, Taesung Kim","doi":"10.1063/5.0169555","DOIUrl":"https://doi.org/10.1063/5.0169555","url":null,"abstract":"Gas-permeable membranes (GPMs) and membrane-like micro-/nanostructures offer precise control over the transport of liquids, gases, and small molecules on microchips, which has led to the possibility of diverse applications, such as gas sensors, solution concentrators, and mixture separators. With the escalating demand for GPMs in microfluidics, this Perspective article aims to comprehensively categorize the transport mechanisms of gases through GPMs based on the penetrant type and the transport direction. We also provide a comprehensive review of recent advancements in GPM-integrated microfluidic devices, provide an overview of the fundamental mechanisms underlying gas transport through GPMs, and present future perspectives on the integration of GPMs in microfluidics. Furthermore, we address the current challenges associated with GPMs and GPM-integrated microfluidic devices, taking into consideration the intrinsic material properties and capabilities of GPMs. By tackling these challenges head-on, we believe that our perspectives can catalyze innovative advancements and help meet the evolving demands of microfluidic applications.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138457636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0