Biomicrofluidics最新文献_第8页

The mechanism of biofilm detachment in porous medium under flow field 流场作用下多孔介质中生物膜脱落的机理

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-05-06 DOI: 10.1063/5.0203061

Yangyang Tang, Zheng Zhang, Cong Tao, Xiaoling Wang

{"title":"The mechanism of biofilm detachment in porous medium under flow field","authors":"Yangyang Tang, Zheng Zhang, Cong Tao, Xiaoling Wang","doi":"10.1063/5.0203061","DOIUrl":"https://doi.org/10.1063/5.0203061","url":null,"abstract":"Biofilms are communities formed by bacteria adhering to surfaces, widely present in porous medium, and their growth can lead to clogging. Our experiment finds that under certain flow conditions, biofilms detach in pores and form a dynamically changing flow path. We define detachment that occurs far from the boundary of the flow path (with a distance greater than 200 μm) as internal detachment and detachment that occurs at the boundary of the flow path as external detachment. To understand the mechanism of biofilm detachment, we study the detachment behaviors of the Bacillus subtilis biofilm in a porous medium in a microfluidic device, where Bacillus subtilis strain is triple fluorescent labeled, which can represent three main phenotypes during the biofilm formation: motile cells, matrix-producing cells, and spores. We find that slow small-scale internal detachment occurs in regions with very few motile cells and matrix-producing cells, and bacterial movement in these areas is disordered. The increase in the number of matrix-producing cells induces clogging, and after clogging, the rapid detachment of the bulk internal biofilm occurs due to the increased pressure difference at the inlet and outlet. When both internal and external detachments occur simultaneously, the number of matrix-producing cells in the internal detachment area is 2.5 times that in the external detachment area. The results indicate that biofilm detachment occurs in areas with fewer matrix-producing cells, as matrix-producing cells can help resist detachment by secreting extracellular polymeric substances (EPSs).","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"115 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140885371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mimicking blood and lymphatic vasculatures using microfluidic systems. 利用微流体系统模拟血液和淋巴管。

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-05-06 eCollection Date: 2024-05-01 DOI: 10.1063/5.0175154

Eva Hall, Kailee Mendiola, N Keilany Lightsey, Donny Hanjaya-Putra

{"title":"Mimicking blood and lymphatic vasculatures using microfluidic systems.","authors":"Eva Hall, Kailee Mendiola, N Keilany Lightsey, Donny Hanjaya-Putra","doi":"10.1063/5.0175154","DOIUrl":"10.1063/5.0175154","url":null,"abstract":"The role of the circulatory system, containing the blood and lymphatic vasculatures, within the body, has become increasingly focused on by researchers as dysfunction of either of the systems has been linked to serious complications and disease. Currently, in vivo models are unable to provide the sufficient monitoring and level of manipulation needed to characterize the fluidic dynamics of the microcirculation in blood and lymphatic vessels; thus in vitro models have been pursued as an alternative model. Microfluidic devices have the required properties to provide a physiologically relevant circulatory system model for research as well as the experimental tools to conduct more advanced research analyses of microcirculation flow. In this review paper, the physiological behavior of fluid flow and electrical communication within the endothelial cells of the systems are detailed and discussed to highlight their complexities. Cell co-culturing methods and other relevant organ-on-a-chip devices will be evaluated to demonstrate the feasibility and relevance of the in vitro microfluidic model. Microfluidic systems will be determined as a noteworthy model that can display physiologically relevant flow of the cardiovascular and lymphatic systems, which will enable researchers to investigate the systems' prevalence in diseases and identify potential therapeutics.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 3","pages":"031502"},"PeriodicalIF":3.2,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11081709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic paper analytic device (μPAD) technology for food safety applications. 用于食品安全应用的微流控纸质分析装置（μPAD）技术。

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-05-02 eCollection Date: 2024-05-01 DOI: 10.1063/5.0192295

Soja Saghar Soman, Shafeek Abdul Samad, Priyamvada Venugopalan, Nityanand Kumawat, Sunil Kumar

{"title":"Microfluidic paper analytic device (μPAD) technology for food safety applications.","authors":"Soja Saghar Soman, Shafeek Abdul Samad, Priyamvada Venugopalan, Nityanand Kumawat, Sunil Kumar","doi":"10.1063/5.0192295","DOIUrl":"10.1063/5.0192295","url":null,"abstract":"Foodborne pathogens, food adulterants, allergens, and toxic chemicals in food can cause major health hazards to humans and animals. Stringent quality control measures at all stages of food processing are required to ensure food safety. There is, therefore, a global need for affordable, reliable, and rapid tests that can be conducted at different process steps and processing sites, spanning the range from the sourcing of food to the end-product acquired by the consumer. Current laboratory-based food quality control tests are well established, but many are not suitable for rapid on-site investigations and are costly. Microfluidic paper analytical devices (μPADs) are a fast-growing field in medical diagnostics that can fill these gaps. In this review, we describe the latest developments in the applications of microfluidic paper analytic device (μPAD) technology in the food safety sector. State-of-the-art μPAD designs and fabrication methods, microfluidic assay principles, and various types of μPAD devices with food-specific applications are discussed. We have identified the prominent research and development trends and future directions for maximizing the value of microfluidic technology in the food sector and have highlighted key areas for improvement. We conclude that the μPAD technology is promising in food safety applications by using novel materials and improved methods to enhance the sensitivity and specificity of the assays, with low cost.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 3","pages":"031501"},"PeriodicalIF":3.2,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11068414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bionic mimosa soft robot based on a multi-responsive PNIPAM-PEGDA hydrogel composition. 基于多反应 PNIPAM-PEGDA 水凝胶成分的仿生含羞草软机器人。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-05-01 DOI: 10.1063/5.0203482

Wenguang Yang, Xiaowen Wang, Xiangyu Teng, Zezheng Qiao, Haibo Yu, Zheng Yuan

{"title":"A bionic mimosa soft robot based on a multi-responsive PNIPAM-PEGDA hydrogel composition.","authors":"Wenguang Yang, Xiaowen Wang, Xiangyu Teng, Zezheng Qiao, Haibo Yu, Zheng Yuan","doi":"10.1063/5.0203482","DOIUrl":"10.1063/5.0203482","url":null,"abstract":"Deformation plays a vital role in the survival of natural organisms. One example is that plants deform themselves to face the sun for sufficient sunlight exposure, which allows them to produce nutrients through photosynthesis. Drawing inspiration from nature, researchers have been exploring the development of 3D deformable materials. However, the traditional approach to manufacturing deformable hydrogels relies on complex technology, which limits their potential applications. In this study, we simulate the stress variations observed in the plant tissue to create a 3D structure from a 2D material. Using UV curing technology, we create a single-layer poly(N-isopropylacrylamide) hydrogel sheet with microchannels that exhibit distinct swelling rates when subjected to stimulation. After a two-step curing process, we produce a poly(N-isopropylacrylamide)-polyethylene glycol diacrylatedouble-layer structure that can be manipulated to change its shape by controlling the light and solvent content. Based on the double-layer structure, we fabricate a dual-response driven bionic mimosa robot that can perform a variety of functions. This soft robot can not only reversibly change its shape but also maintain a specific shape without continuous stimulation. Its capacity for reversible deformation, resulting from internal stress, presents promising application prospects in the biomedical and soft robotics domain. This study delivers an insightful framework for the development of programmable soft materials.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 3","pages":"034102"},"PeriodicalIF":2.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical simulation of cellular blood flow in curved micro-vessels with saccular aneurysms: Effect of curvature degree and hematocrit level 带有囊状动脉瘤的弯曲微血管中细胞血流的数值模拟：弯曲度和血细胞比容水平的影响

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-04-29 DOI: 10.1063/5.0203220

Ahmed Elhanafy, Samir Elsagheer, Shinichi Ookawara, Sameh Nada

{"title":"Numerical simulation of cellular blood flow in curved micro-vessels with saccular aneurysms: Effect of curvature degree and hematocrit level","authors":"Ahmed Elhanafy, Samir Elsagheer, Shinichi Ookawara, Sameh Nada","doi":"10.1063/5.0203220","DOIUrl":"https://doi.org/10.1063/5.0203220","url":null,"abstract":"The dynamics of cellular blood flow in curved vessels considerably differ from those in straight vessels. It is reported that clotting development is significantly affected by vessel shape irregularities. Thus, the current study aims to investigate the effect of curvature degree and hematocrit level on cellular blood flow in a curved micro-vessel with a saccular aneurysm. Accordingly, a three-dimensional numerical simulation is performed using a validated code developed for cellular blood flow problems. The obtained results show that the cell-free layer thickness is highly dependent on the curvature degree and hematocrit level, which may have a remarkable impact on the apparent viscosity of blood as well as the dynamics of other particles such as drug particulates. The near-wall region exhibits the highest degree of cell deformation, whereas the red blood cells within the aneurysm zone remain nearly undeformed. Meanwhile, the velocity of the red blood cells decreases with the increase in curvature degree, which can affect the quality of the oxygenation process. Because of the saccular aneurysm, a considerable decrease in plasma velocity is predicted. Moreover, no secondary flows are detected in the curved vessel except in the aneurysm zone. An increase in the curvature degree is expected to reduce the blood flow rate by about 10%. Furthermore, low wall shear stress values are predicted in the straight case compared to the values at the apex of the curved vessel, which may affect the structure and function of the endothelial cells of the vessel wall and, hence, increase the aneurysm rupture possibility.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"53 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140829584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A brief overview of passive microvalves in microfluidics: Mechanism, manufacturing, and applications 微流体中的无源微阀概述：机理、制造和应用

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-04-22 DOI: 10.1063/5.0188807

Bin Li, Ludan Zhang, Siwei Bai, Jing Jin, Huaying Chen

{"title":"A brief overview of passive microvalves in microfluidics: Mechanism, manufacturing, and applications","authors":"Bin Li, Ludan Zhang, Siwei Bai, Jing Jin, Huaying Chen","doi":"10.1063/5.0188807","DOIUrl":"https://doi.org/10.1063/5.0188807","url":null,"abstract":"Microvalves play a crucial role in manipulating fluid states within a microfluidic system and are finding widespread applications in fields such as biology, medicine, and environmental preservation. Leveraging the characteristics and features of microvalves enables the realization of various complicated microfluidic functions. Continuous advancement in the manufacturing process contributes to more flexible control modes for passive microvalves. As a consequence, these valves are progressively shrinking in size while simultaneously improving in precision and stability. Although active microvalves have the benefits of low leakage, rapid response time, and wide adaptability range, the energy supply system limits the size and even their applicability in integration and miniaturization. In comparison, passive microvalves have the advantage of relying solely on the fluid flow or fluid driving pressure to control the open/close of fluid flow over active microvalves, in spite of having slightly reduced control accuracy. Their self-sustaining feature is highly consistent with the need for assembly and miniaturization in the point-of-care testing technology. Hence, these valves have attracted significant interest for research and application purposes. This review focuses on the recent literature on passive microvalves and details existing passive microvalves from three different aspects: operating principle, processing method, and applications. This work aims to increase the visibility of passive microvalves among researchers and enhance their comprehension by classifying them according to the aforementioned three aspects, facilitating the practical applications and further developments of passive microvalves. Additionally, this paper is expected to serve as a comprehensive and systematic reference for interdisciplinary researchers that intend to design related microfluidic systems.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"2019 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acoustohydrodynamic micromixers: Basic mixing principles, programmable mixing prospectives, and biomedical applications 声流体动力微搅拌器：基本混合原理、可编程混合前景和生物医学应用

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-04-18 DOI: 10.1063/5.0179750

Chenhao Bai, Xiaoqing Tang, Yuyang Li, Tatsuo Arai, Qiang Huang, Xiaoming Liu

{"title":"Acoustohydrodynamic micromixers: Basic mixing principles, programmable mixing prospectives, and biomedical applications","authors":"Chenhao Bai, Xiaoqing Tang, Yuyang Li, Tatsuo Arai, Qiang Huang, Xiaoming Liu","doi":"10.1063/5.0179750","DOIUrl":"https://doi.org/10.1063/5.0179750","url":null,"abstract":"Acoustohydrodynamic micromixers offer excellent mixing efficiency, cost-effectiveness, and flexible controllability compared with conventional micromixers. There are two mechanisms in acoustic micromixers: indirect influence by induced streamlines, exemplified by sharp-edge micromixers, and direct influence by acoustic waves, represented by surface acoustic wave micromixers. The former utilizes sharp-edge structures, while the latter employs acoustic wave action to affect both the fluid and its particles. However, traditional micromixers with acoustic bubbles achieve significant mixing performance and numerous programmable mixing platforms provide excellent solutions with wide applicability. This review offers a comprehensive overview of various micromixers, elucidates their underlying principles, and explores their biomedical applications. In addition, advanced programmable micromixing with impressive versatility, convenience, and ability of cross-scale operations is introduced in detail. We believe this review will benefit the researchers in the biomedical field to know the micromixers and find a suitable micromixing method for their various applications.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"9 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140625766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time-traceable micro-taggants for anti-counterfeiting and secure distribution of food and medicines 用于食品和药品防伪和安全分销的时间可追踪微型掺杂物

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-04-15 DOI: 10.1063/5.0200915

Cheolheon Park, Yunjin Jeong, Huiran Yeom, Seo Woo Song, Wook Park, Daewon Lee

引用次数: 0

Soluto-thermal Marangoni convection in stationary micro-bioreactors on heated substrates: Tool for in vitro diagnosis of PSA 加热基底上固定微型生物反应器中的溶解-热马兰戈尼对流：PSA 体外诊断工具

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-04-08 DOI: 10.1063/5.0188093

Vidisha Singh Rathaur, Siddhartha Panda

{"title":"Soluto-thermal Marangoni convection in stationary micro-bioreactors on heated substrates: Tool for in vitro diagnosis of PSA","authors":"Vidisha Singh Rathaur, Siddhartha Panda","doi":"10.1063/5.0188093","DOIUrl":"https://doi.org/10.1063/5.0188093","url":null,"abstract":"The investigation of antigen-laden droplet deposition patterns on antibody-immobilized substrates has potential for disease detection. Stationary droplets that contain antigens on surfaces immobilized with antibodies can function as microreactors. Temperature modulation enhances reaction efficiency and reduces detection time in droplet-based systems. Thus, the aim of this study is to explore the impact of substrate heating on the structures of protein deposits and the influence of substrate temperature on thermo-solutal Marangoni convection within the droplets. Previous research has explored deposition patterns as diagnostic tools, but limited investigations have focused on the effects of substrate heating on protein deposit structures and the influence of substrate temperature on thermo-solutal Marangoni convection within droplets, creating a knowledge gap. In this study, we conducted experiments to explore how heating the substrate affects the deposition patterns of droplets containing prostate-specific antigen (PSA) on a substrate immobilized with anti-PSA IgG. Additionally, we investigated the thermo-solutal Marangoni convection within these droplets. Our findings reveal distinct deposition patterns classified into dendritic structures (heterogeneous), transitional patterns, and needle-like (homogeneous) structures. The presence of prominent coffee rings and the variation in crystal size across different groups highlight the interplay between thermal and solutal Marangoni advection. Entropy analysis provides insights into structural differences within and between patterns. This work optimizes substrate temperatures for reduced evaporation and detection times while preserving protein integrity, advancing diagnostic tool development, and improving understanding of droplet-based systems.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"67 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spheroids formation in large drops suspended in superhydrophobic paper cones 悬浮在超疏水纸锥中的大水滴形成球状物

IF 3.2 4区工程技术

Biomicrofluidics Pub Date : 2024-04-05 DOI: 10.1063/5.0197807

Omkar Mohapatra, Maheshwar Gopu, Rahail Ashraf, Jijo Easo George, Saniya Patil, Raju Mukherjee, Sanjay Kumar, Dileep Mampallil

引用次数: 0