Manipulation of magnetic beads for actively capturing Vibrio parahaemolyticus and nucleic acid based on microfluidic system.

IF 2.6 4区工程技术 Q2 BIOCHEMICAL RESEARCH METHODS

Biomicrofluidics Pub Date : 2024-05-10 eCollection Date: 2024-05-01 DOI:10.1063/5.0193442

Zhaoxuan Zhang, Xue Deng, Wenqiang Zhang, Kehan Chen, Yuan Su, Chao Gao, De Gong, Longjiao Zhu, Jun Cai

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引用次数: 0

Abstract

Rapid biological detection of pathogen micro-organisms has attracted much attention for practical biomedical applications. Despite the development in this field, it is still challenging to achieve simple and rapid biological detection using the microfluidic method. Herein, we propose a novel strategy of biological detection that combines precise detection control of the capillary microfluidic chip and versatile manipulation of magnetic beads. The microfluidic chip was fabricated via laser cutting, which utilized capillary pressure to realize rapid passive injection of liquid samples. Under an external magnetic field, the aptamer-modified magnetic beads were actuated to mix with Vibrio parahaemolyticus (V. parahaemolyticus) and its nucleic acid in the capillary microfluidic chip for rapid selective capture and detection, which could be achieved within 40 min. The experimental results demonstrated that V. parahaemolyticus could be captured using on-chip immunomagnetic beads with a high efficiency and significantly enhanced detection value. Due to these superior performances, the capillary microfluidic system, based on the manipulation of magnetic beads, demonstrated great potential for automatic biological detection.

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基于微流体系统的磁珠主动捕获副溶血性弧菌和核酸的操作。

病原微生物的快速生物检测在实际生物医学应用中备受关注。尽管这一领域在不断发展，但利用微流体方法实现简单快速的生物检测仍具有挑战性。在此，我们提出了一种新型生物检测策略，将毛细管微流控芯片的精确检测控制与磁珠的多功能操作相结合。微流控芯片通过激光切割制作而成，利用毛细管压力实现液体样品的快速被动注入。在外加磁场的作用下，合子修饰的磁珠被驱动与毛细管微流控芯片中的副溶血弧菌及其核酸混合，实现快速选择性捕获和检测，整个过程可在 40 分钟内完成。实验结果表明，使用芯片上的免疫磁珠可高效捕获副溶血性弧菌，并显著提高检测值。由于这些优异的性能，基于磁珠操作的毛细管微流控系统在生物自动检测方面展现出巨大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomicrofluidics 生物-纳米科技

CiteScore

5.80

自引率

3.10%

发文量

审稿时长

1.3 months

期刊介绍： Biomicrofluidics (BMF) is an online-only journal published by AIP Publishing to rapidly disseminate research in fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena. BMF also publishes research in unique microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. BMF offers quick publication, multimedia capability, and worldwide circulation among academic, national, and industrial laboratories. With a primary focus on high-quality original research articles, BMF also organizes special sections that help explain and define specific challenges unique to the interdisciplinary field of biomicrofluidics. Microfluidic and nanofluidic actuation (electrokinetics, acoustofluidics, optofluidics, capillary) Liquid Biopsy (microRNA profiling, circulating tumor cell isolation, exosome isolation, circulating tumor DNA quantification) Cell sorting, manipulation, and transfection (di/electrophoresis, magnetic beads, optical traps, electroporation) Molecular Separation and Concentration (isotachophoresis, concentration polarization, di/electrophoresis, magnetic beads, nanoparticles) Cell culture and analysis(single cell assays, stimuli response, stem cell transfection) Genomic and proteomic analysis (rapid gene sequencing, DNA/protein/carbohydrate arrays) Biosensors (immuno-assay, nucleic acid fluorescent assay, colorimetric assay, enzyme amplification, plasmonic and Raman nano-reporter, molecular beacon, FRET, aptamer, nanopore, optical fibers) Biophysical transport and characterization (DNA, single protein, ion channel and membrane dynamics, cell motility and communication mechanisms, electrophysiology, patch clamping). Etc...