Biomicrofluidics最新文献_第4页

Bio-energy-powered microfluidic devices. 生物能源驱动的微流体装置。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-24 eCollection Date: 2024-12-01 DOI: 10.1063/5.0227248

Yuhan Li, Chuangyi Xu, Yifan Liao, Xiao Chen, Jiang Chen, Fan Yang, Mingyuan Gao

{"title":"Bio-energy-powered microfluidic devices.","authors":"Yuhan Li, Chuangyi Xu, Yifan Liao, Xiao Chen, Jiang Chen, Fan Yang, Mingyuan Gao","doi":"10.1063/5.0227248","DOIUrl":"10.1063/5.0227248","url":null,"abstract":"Bio-microfluidic technologies offer promising applications in diagnostics and therapy, yet they face significant technical challenges, particularly in the need for external power sources, which limits their practicality and user-friendliness. Recent advancements have explored innovative methods utilizing body fluids, motion, and heat to power these devices, addressing the power supply issue effectively. Among these, body-motion and body-heat-powered systems stand out for their potential to create self-sustaining, wearable, and implantable devices. In this Perspective, we focus on the principles and applications of hydrovoltaic cells, biofuel cells, and piezoelectric and triboelectric nanogenerators. Recent strides in energy conversion efficiency, coupled with the development of biocompatible and durable materials, are driving innovation in bio-integrated electronics. Integration with bio-microfluidic platforms further enhances the linkage to the human body and the potential of these devices for personalized healthcare applications. Ongoing research into these areas promises to deliver sustainable and user-friendly solutions for continuous monitoring, diagnostics, and therapy, potentially revolutionizing the landscape of healthcare delivery.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"061303"},"PeriodicalIF":2.6,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Titrating chimeric antigen receptors on CAR T cells enabled by a microfluidic-based dosage-controlled intracellular mRNA delivery platform. 基于微流体的剂量控制细胞内mRNA递送平台在CAR - T细胞上滴定嵌合抗原受体。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1063/5.0231595

Yu-Hsi Chen, Mahnoor Mirza, Ruoyu Jiang, Abraham P Lee

引用次数: 0

Recent developments in microfluidic passive separation to enable purification of platelets for transfusion. 用于输血血小板纯化的微流控被动分离技术的最新进展。

IF 2.4 4区工程技术

Biomicrofluidics Pub Date : 2024-12-17 eCollection Date: 2024-12-01 DOI: 10.1063/5.0226060

Mai T P Dinh, Mubasher Iqbal, Kumar Abhishek, Fong W Lam, Sergey S Shevkoplyas

{"title":"Recent developments in microfluidic passive separation to enable purification of platelets for transfusion.","authors":"Mai T P Dinh, Mubasher Iqbal, Kumar Abhishek, Fong W Lam, Sergey S Shevkoplyas","doi":"10.1063/5.0226060","DOIUrl":"10.1063/5.0226060","url":null,"abstract":"Platelet transfusion is a lifesaving therapy intended to prevent and treat bleeding. However, in addition to platelets, a typical unit also contains a large volume of supernatant that accumulates multiple pro-inflammatory contaminants, including residual leukocytes, microaggregates, microparticles, antibodies, and cytokines. Infusion of this supernatant is responsible for virtually all adverse reactions to platelet transfusions. Conventional methods for removing residual leukocytes (leukoreduction) and reducing the volume of transfused supernatant (volume reduction) struggle to mitigate these risks holistically. Leukoreduction filters can remove leukocytes and microaggregates but fail to reduce supernatant volume, whereas centrifugation can reduce volume, but it is ineffective against larger contaminants and damages platelets. Additionally, platelet purification based on these methods is often too logistically complex, time-consuming, and labor-intensive to implement routinely. Emerging microfluidic technologies offer promising alternatives through passive separation mechanisms that enable cell separation with minimal damage and drastically reduced instrumentation size and facility requirements. This review examines recent innovations in microfluidic cell separation that can be used for leukoreduction and volume reduction of platelets. It begins by defining the performance requirements that any separation method must meet to successfully replace conventional methods currently used to perform these tasks. Standard performance metrics are described, including leukocyte depletion efficiency, degree of volume reduction, processing throughput, and platelet recovery. Finally, the review outlines the primary challenges that must be overcome to enable simple-to-use, disposable microfluidic devices capable of both reducing the platelet unit volume and removing pro-inflammatory contaminants, while preserving most functional platelets for transfusion.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"061504"},"PeriodicalIF":2.4,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanically mediated cargo delivery to cells using microfluidic devices. 利用微流体装置将机械介导的货物输送到细胞。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-06 eCollection Date: 2024-12-01 DOI: 10.1063/5.0240667

Zhiyu Mao, Bori Shi, Jinbo Wu, Xinghua Gao

{"title":"Mechanically mediated cargo delivery to cells using microfluidic devices.","authors":"Zhiyu Mao, Bori Shi, Jinbo Wu, Xinghua Gao","doi":"10.1063/5.0240667","DOIUrl":"10.1063/5.0240667","url":null,"abstract":"Drug delivery technologies, which are a crucial area of research in the field of cell biology, aim to actively or passively deliver drugs to target cells to enhance therapeutic efficacy and minimize off-target effects. In recent years, with advances in drug development, particularly, the increasing demand for macromolecular drugs (e.g., proteins and nucleic acids), novel drug delivery technologies and intracellular cargo delivery systems have emerged as promising tools for cell and gene therapy. These systems include various viral- and chemical-mediated methods as well as physical delivery strategies. Physical methods, such as electroporation and microinjection, have shown promise in early studies but have not been widely adopted due to concerns regarding efficiency and cellular viability. Recently, microfluidic technologies have provided new opportunities for cargo delivery by allowing for precise control of fluid dynamic parameters to achieve efficient and safe penetration of cell membranes, as well as for foreign material transport. Microfluidics-based mechanical delivery methods utilize biophysical phenomena, such as cell constriction and fluid shear, and are associated with high throughput and high transfection efficiency. In this review, we summarize the latest advancements in microfluidic mechanical delivery technologies, and we discuss constriction- and fluid shear-induced delivery strategies. Furthermore, we explore the potential application of artificial intelligence in optimizing cargo delivery technologies, aiming to provide theoretical support and practical guidance for the future development of novel cellular drug delivery technologies.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"061302"},"PeriodicalIF":2.6,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Miniaturized electrophoresis: An integrated microfluidic cartridge with functionalized hydrogel-assisted LAMP for sample-to-answer analysis of nucleic acid. 微型电泳：一个集成的微流体盒与功能化的水凝胶辅助LAMP用于样本到答案的核酸分析。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-12-04 eCollection Date: 2024-12-01 DOI: 10.1063/5.0211812

Natish Kumar, Monika Kumari, Devtulya Chander, Sandeep Dogra, Asha Chaubey, Ravi Kumar Arun

{"title":"Miniaturized electrophoresis: An integrated microfluidic cartridge with functionalized hydrogel-assisted LAMP for sample-to-answer analysis of nucleic acid.","authors":"Natish Kumar, Monika Kumari, Devtulya Chander, Sandeep Dogra, Asha Chaubey, Ravi Kumar Arun","doi":"10.1063/5.0211812","DOIUrl":"10.1063/5.0211812","url":null,"abstract":"Accurate detection of pathogenic nucleic acids is crucial for early diagnosis, effective treatment, and containment of infectious diseases. It facilitates the timely identification of pathogens, aids in monitoring disease outbreaks, and helps prevent the spread of infections within healthcare settings and communities. We developed a multi-layered, paper-based microfluidic and miniaturized electrophoresis system for rapid nucleic acid extraction, separation, amplification, and detection, designed for resource-limited settings. Constructed from acrylic, transparency film, pressure-sensitive adhesion, and Whatman paper using a CO2 laser, the setup simplifies traditional methods and eliminates the need for complex equipment. DNA extraction and purification are achieved using Zweifach-Fung bifurcation and Fahraeus effect principles, with detection via a hydrogel-assisted colorimetric isothermal reverse transcriptase-loop-mediated isothermal amplification technique. The system accurately identified the SARS-CoV-2 N-gene and β-actin human gene, validated by a compact electrophoresis setup. In clinical validation with 12 patient specimens, the system demonstrated a positive predictive agreement of 83.0% and a negative predictive agreement of 100%. The system achieves a limit of detection of 1 copy/μl and can potentially transform nucleic acid detection assays in healthcare settings. This study addresses key challenges in nucleic acid detection, such as ensuring sample quality and quantity, reducing reliance on sophisticated equipment, preventing contamination, simplifying procedures, and providing rapid and accurate diagnostics for emerging pathogens.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"064104"},"PeriodicalIF":2.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11620794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunable motile sperm separation based on sperm persistence in migrating through shear barriers. 基于精子在通过剪切屏障迁移中的持久性的可调运动精子分离。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-11-26 eCollection Date: 2024-12-01 DOI: 10.1063/5.0233544

Mohammadjavad Bouloorchi Tabalvandani, Zahra Saeidpour, Zahra Habibi, Saeed Javadizadeh, Majid Badieirostami

{"title":"Tunable motile sperm separation based on sperm persistence in migrating through shear barriers.","authors":"Mohammadjavad Bouloorchi Tabalvandani, Zahra Saeidpour, Zahra Habibi, Saeed Javadizadeh, Majid Badieirostami","doi":"10.1063/5.0233544","DOIUrl":"10.1063/5.0233544","url":null,"abstract":"Rheotaxis is one of the major migratory mechanisms used in autonomous swimmers such as sperms and bacteria. Here, we present a microfluidic chip using joint rheotaxis and boundary-following behavior that selects sperms based on the motility and persistence. The proposed device consists of a channel decorated with diamond-shaped pillars that create spots of increased velocity field and shear rate. These spots are supposed as hydrodynamic barriers that impede the passage of less motile sperms through the channels, while highly motile sperms were able to overcome the generated barrier and swim through the structures. The proposed device was able to populate the chamber with sorted sperms that were fully viable and motile. The experimental results validated the separation of highly motile sperms with enhanced motility parameters compared with the initial sample. Our device was able to improve linear straight velocity, curvilinear velocity, and average path velocity of the sorted population surpassing 35%, compared with the raw semen. The processing time was also reduced to 20 min.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"064103"},"PeriodicalIF":2.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal bubble single-cell printing chip: High-throughput, wide-field, and efficient. 热泡单细胞打印芯片：高通量、宽视场、高效。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-11-26 eCollection Date: 2024-12-01 DOI: 10.1063/5.0225883

Bo Deng, Kun Wang, Peng Huang, Miaomiao Yang, Demeng Liu, Yimin Guan

{"title":"Thermal bubble single-cell printing chip: High-throughput, wide-field, and efficient.","authors":"Bo Deng, Kun Wang, Peng Huang, Miaomiao Yang, Demeng Liu, Yimin Guan","doi":"10.1063/5.0225883","DOIUrl":"10.1063/5.0225883","url":null,"abstract":"Single-cell printing technology has arisen as a potent instrument for investigating cell biology and disease pathophysiology. Nonetheless, current single-cell printing methodologies are hindered by restricted throughput, a limited field of view, and diminished efficiency. We present an innovative single-cell printing chip that utilizes thermal inkjet technology for single-cell printing, therefore addressing these constraints. We have accomplished high-throughput, wide-field, and efficient single-cell printing by merging a high-density thermal foam-based inkjet nozzle array on a chip with high-speed cameras and computer vision technologies for optical image capture and single-cell identification training. We have shown the efficacy and adaptability of the printing chip by printing various concentrations of Chinese hamster ovary cells and human embryonic kidney 293 cells. The printing of a single 96-well plate is accomplished in 2-3 min, facilitating one-time loading and uninterrupted multi-plate paving. Our thermal bubble single-cell printing chip serves as a viable platform for high-throughput single-cell analysis applications.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"064102"},"PeriodicalIF":2.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data-driven models for microfluidics: A short review. 微流控数据驱动模型：简评。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-11-20 eCollection Date: 2024-12-01 DOI: 10.1063/5.0236407

Yu Chang, Qichen Shang, Zifei Yan, Jian Deng, Guangsheng Luo

引用次数: 0

Applications of microfluidics in mRNA vaccine development: A review. 微流控技术在 mRNA 疫苗开发中的应用：综述。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-11-14 eCollection Date: 2024-12-01 DOI: 10.1063/5.0228447

Ali Fardoost, Koosha Karimi, Harshitha Govindaraju, Pegah Jamali, Mehdi Javanmard

引用次数: 0

Viscoelastic particle focusing and separation in a microfluidic channel with a cruciform section. 具有十字形截面的微流体通道中的粘弹性颗粒聚焦和分离。

IF 2.6 4区工程技术

Biomicrofluidics Pub Date : 2024-11-06 eCollection Date: 2024-12-01 DOI: 10.1063/5.0233177

Jaekyeong Jang, Jiyeon Ahn, Taehoon Kim, Younghak Cho

{"title":"Viscoelastic particle focusing and separation in a microfluidic channel with a cruciform section.","authors":"Jaekyeong Jang, Jiyeon Ahn, Taehoon Kim, Younghak Cho","doi":"10.1063/5.0233177","DOIUrl":"10.1063/5.0233177","url":null,"abstract":"Considerable attention has been given to elasto-inertial microfluidics, which are widely applied for the focusing, sorting, and separation of particles/cells. In this work, we propose a novel yet simple fabrication process for a microchannel with a cruciform section, where elasto-inertial particle focusing is explored in a viscoelastic fluid. SU-8 master molds for polydimethylsiloxane (PDMS) structures were fabricated via standard photolithography, and then plasma bonding, following self-alignment between two PDMS structures, was performed for the formation of a microchannel with a cruciform section. The particle behaviors inside the fabricated microchannel were experimentally investigated for various flow rates and particle sizes and compared with those inside a microchannel with a square cross section. The experimental results revealed that 3D particle focusing was achieved in the center under viscoelastic fluid flow over a wide range of flow rates without any shear thinning. Even for small particles (∼2 μm), single-line particle focusing was observed in the microchannel with a cruciform section but not in a square microchannel with the same hydraulic diameter (Dh = 75 μm). The effects of four reflex angles (270°) on particle focusing were quantitatively evaluated through numerical simulation. The simulation revealed that the migration pattern of particles is governed by the combined effect of the reflex angles and fluid inertia, leading to characteristic particle focusing behavior within the cross section of the cruciform microchannel. These findings agree well with the experimental results, which highlight the superior capability of the cruciform microchannel for inertial particle focusing across a wide range of particle sizes.","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":"18 6","pages":"064101"},"PeriodicalIF":2.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11567695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0