Guillermo Ramirez-Alvarado, Gabriel Garibaldi, Chiraz Toujani, Gongchen Sun
{"title":"原位三维聚合(IS-3DP):采用水性两相系统在微流体通道内形成三维物体。","authors":"Guillermo Ramirez-Alvarado, Gabriel Garibaldi, Chiraz Toujani, Gongchen Sun","doi":"10.1063/5.0226620","DOIUrl":null,"url":null,"abstract":"<p><p>Rapid prototyping and fabrication of microstructure have been revolutionized by 3D printing, especially stereolithography (SLA) based techniques due to the superior spatial resolution they offer. However, SLA-type 3D printing faces intrinsic challenges in multi-material integration and adaptive Z-layer slicing due to the use of a vat and a mechanically controlled Z-layer generation. In this paper, we present the conceptualization of a novel paradigm which uses dynamic and multi-phase laminar flow in a microfluidic channel to achieve fabrication of 3D objects. Our strategy, termed \"<i>in situ</i> 3D polymerization,\" combines <i>in situ</i> polymerization and co-flow aqueous two-phase systems and achieves slicing, polymerization, and layer-by-layer printing of 3D structures in a microchannel. The printing layer could be predicted and controlled solely by programming the fluid input. Our strategy provides generalizability to fit with different light sources, pattern generators, and photopolymers. The integration of the microfluidic channel could enable high-degree multi-material integration without complicated modification of the 3D printer.</p>","PeriodicalId":8855,"journal":{"name":"Biomicrofluidics","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510685/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>In situ</i> 3D polymerization (<i>IS</i>-3DP): Implementing an aqueous two-phase system for the formation of 3D objects inside a microfluidic channel.\",\"authors\":\"Guillermo Ramirez-Alvarado, Gabriel Garibaldi, Chiraz Toujani, Gongchen Sun\",\"doi\":\"10.1063/5.0226620\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Rapid prototyping and fabrication of microstructure have been revolutionized by 3D printing, especially stereolithography (SLA) based techniques due to the superior spatial resolution they offer. However, SLA-type 3D printing faces intrinsic challenges in multi-material integration and adaptive Z-layer slicing due to the use of a vat and a mechanically controlled Z-layer generation. In this paper, we present the conceptualization of a novel paradigm which uses dynamic and multi-phase laminar flow in a microfluidic channel to achieve fabrication of 3D objects. Our strategy, termed \\\"<i>in situ</i> 3D polymerization,\\\" combines <i>in situ</i> polymerization and co-flow aqueous two-phase systems and achieves slicing, polymerization, and layer-by-layer printing of 3D structures in a microchannel. The printing layer could be predicted and controlled solely by programming the fluid input. Our strategy provides generalizability to fit with different light sources, pattern generators, and photopolymers. The integration of the microfluidic channel could enable high-degree multi-material integration without complicated modification of the 3D printer.</p>\",\"PeriodicalId\":8855,\"journal\":{\"name\":\"Biomicrofluidics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-10-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510685/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomicrofluidics\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1063/5.0226620\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomicrofluidics","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1063/5.0226620","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
摘要
三维打印技术,尤其是基于立体光刻技术(SLA)的三维打印技术,因其卓越的空间分辨率而为微观结构的快速原型制作和制造带来了革命性的变化。然而,由于使用大桶和机械控制的 Z 层生成,SLA 型三维打印在多材料集成和自适应 Z 层切片方面面临着固有的挑战。在本文中,我们提出了一种新模式的概念,即利用微流体通道中的动态多相层流来实现三维物体的制造。我们的策略被称为 "原位三维聚合",它结合了原位聚合和共流水性两相系统,在微通道中实现了三维结构的切片、聚合和逐层打印。只需对输入的流体进行编程,就能预测和控制打印层。我们的策略具有通用性,可适用于不同的光源、图案生成器和光聚合物。微流体通道的集成可实现高度的多材料集成,而无需对三维打印机进行复杂的改装。
In situ 3D polymerization (IS-3DP): Implementing an aqueous two-phase system for the formation of 3D objects inside a microfluidic channel.
Rapid prototyping and fabrication of microstructure have been revolutionized by 3D printing, especially stereolithography (SLA) based techniques due to the superior spatial resolution they offer. However, SLA-type 3D printing faces intrinsic challenges in multi-material integration and adaptive Z-layer slicing due to the use of a vat and a mechanically controlled Z-layer generation. In this paper, we present the conceptualization of a novel paradigm which uses dynamic and multi-phase laminar flow in a microfluidic channel to achieve fabrication of 3D objects. Our strategy, termed "in situ 3D polymerization," combines in situ polymerization and co-flow aqueous two-phase systems and achieves slicing, polymerization, and layer-by-layer printing of 3D structures in a microchannel. The printing layer could be predicted and controlled solely by programming the fluid input. Our strategy provides generalizability to fit with different light sources, pattern generators, and photopolymers. The integration of the microfluidic channel could enable high-degree multi-material integration without complicated modification of the 3D printer.
期刊介绍:
Biomicrofluidics (BMF) is an online-only journal published by AIP Publishing to rapidly disseminate research in fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena. BMF also publishes research in unique microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications.
BMF offers quick publication, multimedia capability, and worldwide circulation among academic, national, and industrial laboratories. With a primary focus on high-quality original research articles, BMF also organizes special sections that help explain and define specific challenges unique to the interdisciplinary field of biomicrofluidics.
Microfluidic and nanofluidic actuation (electrokinetics, acoustofluidics, optofluidics, capillary)
Liquid Biopsy (microRNA profiling, circulating tumor cell isolation, exosome isolation, circulating tumor DNA quantification)
Cell sorting, manipulation, and transfection (di/electrophoresis, magnetic beads, optical traps, electroporation)
Molecular Separation and Concentration (isotachophoresis, concentration polarization, di/electrophoresis, magnetic beads, nanoparticles)
Cell culture and analysis(single cell assays, stimuli response, stem cell transfection)
Genomic and proteomic analysis (rapid gene sequencing, DNA/protein/carbohydrate arrays)
Biosensors (immuno-assay, nucleic acid fluorescent assay, colorimetric assay, enzyme amplification, plasmonic and Raman nano-reporter, molecular beacon, FRET, aptamer, nanopore, optical fibers)
Biophysical transport and characterization (DNA, single protein, ion channel and membrane dynamics, cell motility and communication mechanisms, electrophysiology, patch clamping). Etc...