Artificial Cells, Nanomedicine, and Biotechnology最新文献_第3页

An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple-negative breast cancer cells with Psidium guajava peel-derived chitosan nanoparticles. 瓜石榴皮衍生壳聚糖纳米颗粒对MDA-MB-231三阴性乳腺癌细胞的靶向细胞毒性研究

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-10 DOI: 10.1080/21691401.2025.2462333

Vino Udappusamy, Rajan Thinagaran, Vijayakumar Mayakrishnan, Janani Balakarthikeyan, Priya Kannappan, Sameer Al-Ghamdi, Naif Abdurhman Alrudian, Mohammed Saad Alqahtani, Khalid Albasheer, Chandrabose Sureka, Mahmoud H El-Bidawy, Nesreen Alsanousi, Sahar Gamil, Thiyagarajan Ramesh

{"title":"An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple-negative breast cancer cells with Psidium guajava peel-derived chitosan nanoparticles.","authors":"Vino Udappusamy, Rajan Thinagaran, Vijayakumar Mayakrishnan, Janani Balakarthikeyan, Priya Kannappan, Sameer Al-Ghamdi, Naif Abdurhman Alrudian, Mohammed Saad Alqahtani, Khalid Albasheer, Chandrabose Sureka, Mahmoud H El-Bidawy, Nesreen Alsanousi, Sahar Gamil, Thiyagarajan Ramesh","doi":"10.1080/21691401.2025.2462333","DOIUrl":"10.1080/21691401.2025.2462333","url":null,"abstract":"Triple-negative breast cancer (TNBC) is a significant global health issue, with high mortality rates. The chemotherapeutic drugs currently used for TNBC have significant side effects, impacting both normal and cancer cells. In this study, we investigated a potential use of fruit peel extract of Psidium guajava (PGP) encapsulated with chitosan nanoparticles (CSNPs) to combat TNBC. The synthesized PGP-CSNPs were characterized using UV-vis spectroscopy, Fourier transform infra-red (FTIR) spectroscopy, TEM and GC-MS. The maximum loading capacity and encapsulation efficacy of PGP-CSNPs were found to be 72.5 ± 0.49% and 92.9 ± 0.10%, respectively. Furthermore, in vitro cytotoxicity was assessed, and the IC50 value for PGP-CSNPs was 50.13 µg/mL. It was observed that PGP-CSNPs could induce apoptosis in MDA-MB-231 cells in dose-dependent manner. Furthermore, molecular docking was performed for bioactive compounds retrieved from PGP-CSNPs against human tumour suppressor proteins Bcl2, and results showed that the PGP-CSNPs had lower binding energy than cisplatin. This suggests that, the synthesized PGP-CSNPs have the potential to serve as a therapeutic agent for tackling TNBC. However, to validate its efficacy in human therapy, furthermore pre-clinical and clinical procedures should be examined, as this is an ongoing and significant step towards developing an effective and safe anticancer drug.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"43-55"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced cartilage repair using gelatin methacryloyl hydrogels combined with icariin and magnesium-doped bioactive glass. 用明胶甲基丙烯酰水凝胶联合淫羊藿苷和掺镁生物活性玻璃增强软骨修复。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-15 DOI: 10.1080/21691401.2025.2490677

Shiyao Liao, Kai Zhou, Yao Kang, Tingxiao Zhao, Yicheng Lin, Jun Lv, Danjie Zhu

{"title":"Enhanced cartilage repair using gelatin methacryloyl hydrogels combined with icariin and magnesium-doped bioactive glass.","authors":"Shiyao Liao, Kai Zhou, Yao Kang, Tingxiao Zhao, Yicheng Lin, Jun Lv, Danjie Zhu","doi":"10.1080/21691401.2025.2490677","DOIUrl":"https://doi.org/10.1080/21691401.2025.2490677","url":null,"abstract":"Cartilage repair remains challenging due to limited self-healing, poor biocompatibility, and insufficient mechanical properties of current materials. To overcome these issues, we developed a multifunctional composite hydrogel by integrating gelatine methacrylate (GelMA) with magnesium-doped bioactive glass (Mg-BG) and icariin (ICA). SEM analysis revealed that pure GelMA exhibited a highly porous yet loosely organized structure, whereas the addition of Mg-BG and ICA produced a denser, more interconnected porous network that enhances cell adhesion and nutrient diffusion. In vitro, the ICA/Mg-BG/GelMA hydrogel achieved a swelling ratio up to 430% and maintained cell viability above 80% over 5 days. Moreover, qRT-PCR and immunohistochemical analyses demonstrated that the composite hydrogel upregulated chondrogenic markers (SOX9, ACAN, and COL2A1) compared with GelMA alone. Specifically, it downregulates M1 pro-inflammatory markers (CCR7, iNOS, CD86) and upregulates M2 anti-inflammatory markers (ARG1, CD163, CD206), thereby creating a regenerative microenvironment. These results indicate that the synergistic combination of GelMA, Mg-BG, and ICA not only improves the scaffold's mechanical support but also enhances its biological functionality, offering a promising strategy for cartilage repair. Future studies will focus on in vivo validation to further assess its clinical potential.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"181-193"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of mitophagy-related biomarkers in human rheumatoid arthritis using machine learning models. 利用机器学习模型鉴定类风湿关节炎中与线粒体自噬相关的生物标志物。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-16 DOI: 10.1080/21691401.2025.2533361

Jiayi Chen, Zuhai Huang, Chengyu Qin, Zixiang Pang, Yuanming Chen

{"title":"Identification of mitophagy-related biomarkers in human rheumatoid arthritis using machine learning models.","authors":"Jiayi Chen, Zuhai Huang, Chengyu Qin, Zixiang Pang, Yuanming Chen","doi":"10.1080/21691401.2025.2533361","DOIUrl":"https://doi.org/10.1080/21691401.2025.2533361","url":null,"abstract":"Rheumatoid arthritis (RA) is a systemic immune-mediated disease characterized by synovitis and joint cartilage destruction. Although many studies have shown that mitophagy is crucial in the development of bone metabolism disorders, its exact function in rheumatoid arthritis (RA) is still not well understood. This study analysed the GSE77298 dataset from the Gene Expression Omnibus (GEO) to identify differentially expressed genes (DEGs) between rheumatoid arthritis (RA) patients and healthy controls. Mitophagy-related genes (MRGs) were extracted from the literature and screened using bioinformatics techniques, resulting in differentially expressed MRGs (DE-MRGs). The diagnostic value of these genes was assessed using receiver operating characteristic (ROC) curves, and an ANN model was constructed. In the GSE77298 dataset, 267 differentially expressed genes (DEGs) were identified. Weighted gene co-expression network analysis (WGCNA) identified 2191 key module genes, leading to 63 DE-MRGs. Two MRGs, TMEM45A and ZBTB25, were identified as hub genes with areas under the curve (AUC) of 0.991 and 0.911, respectively. The nomogram model demonstrated high diagnostic value. Mitophagy plays a critical role in the progression of rheumatoid arthritis (RA). Identifying two genes associated with mitophagy may aid in the early diagnosis, mechanistic understanding, and treatment of RA.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"287-303"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the impact of gut microbiota-derived metabolites on depression through network pharmacology. 通过网络药理学揭示肠道微生物衍生代谢物对抑郁症的影响。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-24 DOI: 10.1080/21691401.2025.2531752

Si Su, Tengarile A, Ruhan A, Riga Wu, Lisi Wei, La Ta, Wenfeng Huo, Lijun Tong, Jing Zhang, Rilebagen Hu, Li Li

{"title":"Revealing the impact of gut microbiota-derived metabolites on depression through network pharmacology.","authors":"Si Su, Tengarile A, Ruhan A, Riga Wu, Lisi Wei, La Ta, Wenfeng Huo, Lijun Tong, Jing Zhang, Rilebagen Hu, Li Li","doi":"10.1080/21691401.2025.2531752","DOIUrl":"https://doi.org/10.1080/21691401.2025.2531752","url":null,"abstract":"A total of 208 metabolites and 223 targets were initially extracted from the gutMGene v1.0 database. In addition, 1,630 and 1,321 targets were identified using the Similarity Ensemble Approach and Swiss Target Prediction databases, respectively, resulting in 921 overlapping targets. By integrating data from gutMGenev1.0, 13 core targets were finally identified. A microbiota-metabolite-target-signal pathway-disease network was constructed using Cytoscape 3.9.1, revealing 15 metabolites associated with the IL-17, TLR, and PI3K-Akt signalling pathways. Among these, five metabolites exhibited favourable drug similarity and acceptable toxicological profiles. Molecular docking confirmed the stable binding of two key metabolites-succinate and phenylacetylglutamine-to their respective targets. The results showed that succinate and phenylacetylglutamine demonstrated strong binding affinities to IL-1β and GSK3B, both involved in the IL-17, TLR, and PI3K-Akt signalling pathways. IL-17 and TLR4, as important pro-inflammatory cytokines, are closely associated with the development of depression, while the PI3K/AKT signalling pathway plays a key role in its pathogenesis. The present study reveals the potential mechanisms by which gut microbiota influence MDD and provides a scientific basis and a comprehensive research framework for future investigations.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"327-342"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Long non-coding RNA NKILA weakens TNF-α-induced inflammation of MRC-5 cells by miR-21 up-regulation. 撤回：长链非编码RNA NKILA通过上调miR-21减弱TNF-α-诱导的MRC-5细胞炎症。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-08-12 DOI: 10.1080/21691401.2025.2543678

引用次数: 0

Correction. 更正。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-14 DOI: 10.1080/21691401.2025.2478352

引用次数: 0

Statement of Retraction: Long noncoding RNA LINC-PINT inhibits non-small cell lung cancer progression through sponging miR-218-5p/PDCD4. 撤回声明：长链非编码RNA LINC-PINT通过海绵miR-218-5p/PDCD4抑制非小细胞肺癌的进展。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-21 DOI: 10.1080/21691401.2025.2536932

引用次数: 0

In vivo toxicity of chitosan-based nanoparticles: a systematic review. 壳聚糖基纳米颗粒的体内毒性：系统综述。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-09 DOI: 10.1080/21691401.2025.2462328

Shela Salsabila, Miski Aghnia Khairinisa, Nasrul Wathoni, Irna Sufiawati, Wan Ezumi Mohd Fuad, Nur Kusaira Khairul Ikram, Muchtaridi Muchtaridi

{"title":"In vivo toxicity of chitosan-based nanoparticles: a systematic review.","authors":"Shela Salsabila, Miski Aghnia Khairinisa, Nasrul Wathoni, Irna Sufiawati, Wan Ezumi Mohd Fuad, Nur Kusaira Khairul Ikram, Muchtaridi Muchtaridi","doi":"10.1080/21691401.2025.2462328","DOIUrl":"10.1080/21691401.2025.2462328","url":null,"abstract":"Chitosan nanoparticles have been extensively utilised as polymeric drug carriers in nanoparticles formulations due to their potential to enhance drug delivery, efficacy, and safety. Numerous toxicity studies have been previously conducted to assess the safety profile of chitosan-based nanoparticles. These toxicity studies employed various methodologies, including test animals, interventions, and different routes of administration. This review aims to summarise research on the safety profile of chitosan-based nanoparticles in drug delivery, with a focus on general toxicity tests to determine LD50 and NOAEL values. It can serve as a repository and reference for chitosan-based nanoparticles, facilitating future research and further development of drugs delivery system using chitosan nanoparticles. Publications from 2014 to 2024 were obtained from PubMed, Scopus, Google Scholar, and ScienceDirect, in accordance with the inclusion and exclusion criteria.The ARRIVE 2.0 guidelines were employed to evaluate the quality and risk-of-bias in the in vivo toxicity studies. The results demonstrated favourable toxicity profiles, often exhibiting reduced toxicity compared to free drugs or substances. Acute toxicity studies consistently reported high LD50 values, frequently exceeding 5000 mg/kg body weight, while subacute studies typically revealed no significant adverse effects. Various routes of administration varied, including oral, intravenous, intraperitoneal, inhalation, and topical, each demonstrating promising safety profiles.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"1-15"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-08-01 DOI: 10.1080/21691401.2025.2538368

引用次数: 0

Antiparasitic and antimicrobial activity of Ipomoea palmata against Toxoplasma gondii and Staphylococcus aureus: correlation with major phenolics identified by HPLC. 棕榈木对刚地弓形虫和金黄色葡萄球菌的抗寄生和抗菌活性：与HPLC鉴定的主要酚类物质的相关性。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-26 DOI: 10.1080/21691401.2025.2494796

Amira Mira, Tariq M Aljarba, Fatma M Abdel Bar, Rehab Ahmed, Walaa A Negm, Engy Elekhnawy, Hosam M El-Seadawy, Eman A Elmorsy, Salwa S Younis

{"title":"Antiparasitic and antimicrobial activity of Ipomoea palmata against Toxoplasma gondii and Staphylococcus aureus: correlation with major phenolics identified by HPLC.","authors":"Amira Mira, Tariq M Aljarba, Fatma M Abdel Bar, Rehab Ahmed, Walaa A Negm, Engy Elekhnawy, Hosam M El-Seadawy, Eman A Elmorsy, Salwa S Younis","doi":"10.1080/21691401.2025.2494796","DOIUrl":"https://doi.org/10.1080/21691401.2025.2494796","url":null,"abstract":"Toxoplasma gondii, a protozoan parasite found in water sources, causes toxoplasmosis, with no current protocols for inactivating its oocysts in water. Staphylococcus aureus, a significant bacterial pathogen, is known for causing various illnesses, including skin infections and biofilm-related diseases. This study investigated the antibacterial and antiparasitic properties of Ipomoea palmata leaf extract, rich in phenolics, against T. gondii tachyzoites and S. aureus. I. palmata extract significantly reduced tachyzoites count in peritoneal fluids and liver smears of infected mice with alleviation of toxoplasmosis-induced hepatitis. SEM showed surface irregularities in tachyzoites from treated groups. The extract demonstrated antibacterial action against S. aureus with a minimum inhibitory concentration of 128 to 512 µg/mL, reduced biofilm formation from 69.23% to 15.38% of tested isolates, and downregulated biofilm genes (cna, fnbA, and ica) in 53.85% of isolates. Treatment with I. palmata extract improved liver architecture, reduced inflammation, and eliminated blood vessel congestion. The main phenolic acids identified by HPLC/UV analysis were chlorogenic acid, gallic acid, ellagic acid, and methyl gallate, while the predominant flavonoids were apigenin, quercetin, and naringenin. These findings highlight the potential of I. palmata extract as a natural antimicrobial and antiparasitic agent, warranting further research to isolate and evaluate its active compounds.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"194-206"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0