Artificial Cells, Nanomedicine, and Biotechnology最新文献

Correction. 修正。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-07 DOI: 10.1080/21691401.2025.2476906

引用次数: 0

Statement of Retraction: Myricetin nanoliposomes induced SIRT3-mediated glycolytic metabolism leading to glioblastoma cell death. 撤回声明：杨梅素纳米脂质体诱导sirt3介导的糖酵解代谢导致胶质母细胞瘤细胞死亡。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-27 DOI: 10.1080/21691401.2025.2465942

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Human tendon stem/progenitor cell-derived extracellular vesicle production promoted by dynamic culture. 动态培养促进人肌腱干/祖细胞来源的细胞外囊泡生成。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/21691401.2025.2475099

Marta Clerici, Maria Camilla Ciardulli, Erwin Pavel Lamparelli, Joseph Lovecchio, Emanuele Giordano, Tina P Dale, Nicholas R Forsyth, Nicola Maffulli, Giovanna Della Porta

{"title":"Human tendon stem/progenitor cell-derived extracellular vesicle production promoted by dynamic culture.","authors":"Marta Clerici, Maria Camilla Ciardulli, Erwin Pavel Lamparelli, Joseph Lovecchio, Emanuele Giordano, Tina P Dale, Nicholas R Forsyth, Nicola Maffulli, Giovanna Della Porta","doi":"10.1080/21691401.2025.2475099","DOIUrl":"https://doi.org/10.1080/21691401.2025.2475099","url":null,"abstract":"Tendon injuries significantly impact quality of life, prompting the exploration of innovative solutions beyond conventional surgery. Extracellular Vesicles (EVs) have emerged as a promising strategy to enhance tendon regeneration. In this study, human Tendon Stem/Progenitor Cells (TSPCs) were isolated from surgical biopsies and cultured in a Growth-Differentiation Factor-5-supplemented medium to promote tenogenic differentiation under static and dynamic conditions using a custom-made perfusion bioreactor. Once at 80% confluence, cells were transitioned to a serum-free medium for conditioned media collection. Ultracentrifugation revealed the presence of vesicles with a 106 particles/mL concentration and sub-200nm diameter size. Dynamic culture yielded a 3-fold increase in EV protein content compared to static culture, as confirmed by Western-blot analysis. Differences in surface marker expression were also shown by flow cytometric analysis. Data suggest that we efficiently developed a protocol for extracting EVs from human TSPCs, particularly under dynamic conditions. This approach enhances EV protein content, offering potential therapeutic benefits for tendon regeneration. However, further research is needed to fully understand the role of EVs in tendon regeneration.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"1-16"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The integration of metabolites from Forsythia suspensa and gut microbiota ameliorates drug-induced liver injury: network pharmacology and molecular docking studies. 连翘代谢物与肠道微生物群的整合改善药物性肝损伤：网络药理学和分子对接研究。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-07 DOI: 10.1080/21691401.2025.2475088

Yanni Wang, Xiangxiang Peng, Bingjie Qian, Libo Wang, Jiabing Wang

{"title":"The integration of metabolites from Forsythia suspensa and gut microbiota ameliorates drug-induced liver injury: network pharmacology and molecular docking studies.","authors":"Yanni Wang, Xiangxiang Peng, Bingjie Qian, Libo Wang, Jiabing Wang","doi":"10.1080/21691401.2025.2475088","DOIUrl":"https://doi.org/10.1080/21691401.2025.2475088","url":null,"abstract":"This study integrates metabolites from Forsythia suspensa (FS) and gut microbiota GM to assess combined therapeutic efficacy against drug-induced liver injury (DILI) using network pharmacology and molecular docking. Metabolites of FS and GM were retrieved from the NPASS and gutMGene databases, respectively. Relevant targets for metabolites and DILI-related targets were identified through public databases. The PPI network and KEGG pathway analysis were employed to identify hub targets and key signalling pathways. Furthermore, we performed a molecular docking assay on the active metabolites and targets to verify the network pharmacological concept. The physicochemical properties and toxicity of identified key metabolites were assessed using in silico platforms. 19 final targets were recognized as key proteins responsible for the alleviation of DILI by FS and GM metabolites, with ESR1 emerging as a central target in the PPI network. The estrogen signalling pathway, particularly involving ESR1, ESR2 and JUN genes, was identified as a key mediator in the therapeutic effects. Four GM metabolites (baicalein, luteolin, lunularin and 2,3-bis(3,4-dihydroxybenzyl)butyrolactone) and two FS metabolites (pinoresinol and isolariciresinol) were identified as non-toxic, promising candidates. In conclusion, metabolites from FS and GM may exert a potent synergistic effect on DILI through modulation of the estrogen signalling pathway.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"105-121"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-04 DOI: 10.1080/21691401.2025.2473244

引用次数: 0

Antimicrobial capping agents on silver nanoparticles made via green method using natural products from banana plant waste. 利用香蕉植物废料中的天然产物，通过绿色方法制备纳米银抗菌封盖剂。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-07 DOI: 10.1080/21691401.2025.2462335

Jimmy K Kabeya, Nadège K Ngombe, Paulin K Mutwale, Justin B Safari, Gauta Gold Matlou, Rui W M Krause, Christian I Nkanga

{"title":"Antimicrobial capping agents on silver nanoparticles made via green method using natural products from banana plant waste.","authors":"Jimmy K Kabeya, Nadège K Ngombe, Paulin K Mutwale, Justin B Safari, Gauta Gold Matlou, Rui W M Krause, Christian I Nkanga","doi":"10.1080/21691401.2025.2462335","DOIUrl":"10.1080/21691401.2025.2462335","url":null,"abstract":"Herein, we investigated the phytochemical composition and antibacterial activities of the organic layers from biosynthesized silver nanoparticles (AgNPs). AgNPs were synthesized using Musa paradisiaca and Musa sapientum extracts. UV-vis absorption in the 400-450 nm range indicated surface plasmonic resonance peak of AgNPs. Samples analyses using dynamic light scattering and transmission electron microscopy revealed the presence of particles within nanometric ranges, with sizes of 30-140 nm and 8-40 nm, respectively. Fourier transform infrared (FTIR) unveiled the presence of several organic functional groups on the surface of AgNPs, indicating the presence of phytochemicals from plant extracts. Thin layer chromatography (TLC) of the phytochemicals (capping agents) from AgNPs identified multiple groups of secondary metabolites. These phytochemical capping agents exhibited antibacterial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, with minimum inhibitory concentrations ranging from 62.5 to 1000 µg/mL. Regardless of the bacterial species or plant parts (leaves or pseudo-stems), capping agents from M. sapientum nanoparticles displayed significantly enhanced antibacterial effectiveness compared to all other samples, including the raw plant extracts and biosynthesized capped and uncapped AgNPs. These results suggest the presence of antimicrobial phytochemicals on biosynthesized AgNPs, highlighting the promise of green nanoparticle synthesis as a valuable approach in bioprospecting antimicrobial agents.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"29-42"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Microvesicles and exosomes isolated from murine bone marrow-derived mesenchymal stromal cells primed with p38MAPK inhibitor differentially regulate hematopoietic stem cell function. 从小鼠骨髓间充质基质细胞中分离的微囊泡和外泌体对造血干细胞功能的调控存在差异。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/21691401.2025.2475095

Pallavi Budgude, Vaijayanti Kale, Anuradha Vaidya

{"title":"Microvesicles and exosomes isolated from murine bone marrow-derived mesenchymal stromal cells primed with p38MAPK inhibitor differentially regulate hematopoietic stem cell function.","authors":"Pallavi Budgude, Vaijayanti Kale, Anuradha Vaidya","doi":"10.1080/21691401.2025.2475095","DOIUrl":"https://doi.org/10.1080/21691401.2025.2475095","url":null,"abstract":"The signaling mechanisms active within mesenchymal stromal cells (MSCs) influence the composition of microvesicles (MVs) and exosomes (Exos) secreted by them. Previously, we showed that priming MSCs with a p38 pharmacological inhibitor (pMSCs) rejuvenates them and improves their ability to promote ex vivo hematopoietic stem cell (HSC) expansion. This study examined whether pMSCs exerted HSC-supportive ability via MVs (pMVs) and Exos (pExos). Our findings demonstrate distinct regulation of HSC fate by pMVs and pExos. pMVs promoted the expansion of long-term HSCs (LT-HSCs), distinguished by their robust self-renewal capacity and superior engraftment ability. In contrast, pExos facilitated expansion of short-term HSCs (ST-HSCs) with high proliferative and differentiation potential. Infusing a combination of pMVs- and pExos-expanded HSCs as a composite graft resulted in significantly higher HSC engraftment, emphasizing the synergistic interaction between LT- and ST-HSC populations. Gene expression studies, functional and phenotypic experiments showed that pMVs regulate HSC quiescence via the Egr1/Cdkn1a axis, while pExos control HSC proliferation via the Nfya/Cdkn1a axis. These findings provide insights into the molecular mechanisms underlying the differential regulation of HSC function by pMVs and pExos. It also proposes a composite graft strategy of using pMVs and pExos as \"MSC-derived biologics\" for improving the HSC transplantation success.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"122-137"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis of silver nanoparticles for functional cotton fabrics: antimicrobial efficacy against multidrug-resistant bacteria and cytotoxicity evaluation. 功能棉织物用纳米银的绿色合成：对多重耐药细菌的抗菌效果及细胞毒性评价。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-01 DOI: 10.1080/21691401.2025.2485115

Sérgio Antunes Filho, Clara M Almeida, Maria Teresa Villela Romanos, Bianca Pizzorno Backx, Raquel Regina Bonelli

{"title":"Green synthesis of silver nanoparticles for functional cotton fabrics: antimicrobial efficacy against multidrug-resistant bacteria and cytotoxicity evaluation.","authors":"Sérgio Antunes Filho, Clara M Almeida, Maria Teresa Villela Romanos, Bianca Pizzorno Backx, Raquel Regina Bonelli","doi":"10.1080/21691401.2025.2485115","DOIUrl":"https://doi.org/10.1080/21691401.2025.2485115","url":null,"abstract":"Bacterial infections associated with healthcare are a challenge on a global scale due to the high morbidity and mortality rates, especially those caused by multidrug-resistant isolates. Hospital textiles are abiotic surfaces that may serve as a means of disseminating and persisting microorganisms in hospitals, as microorganisms can remain viable on these surfaces for up to months. In this study, we employed a green synthesis approach utilizing guava leaf extract (Psidium guajava) to produce silver nanoparticles, which were then incorporated into a cotton fabric. Antimicrobial properties and the cytotoxicity of the functional textile were assessed. The finding indicated that the green synthesis method was efficient and resulted in a predominant population of nanoparticles with diameters ranging from 25 to 84 nm that were uniformly dispersed in the textile. The functional textile exhibited low toxicity and high antimicrobial efficiency, even against multidrug-resistant microorganisms of particular concern in hospital settings. Atomic force microscopy carried out evidenced invaginations in the cell wall of bacteria submitted to this textile, suggesting surface damage as an important mechanism of action silver nanoparticles incorporated.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"153-165"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple-negative breast cancer cells with Psidium guajava peel-derived chitosan nanoparticles. 瓜石榴皮衍生壳聚糖纳米颗粒对MDA-MB-231三阴性乳腺癌细胞的靶向细胞毒性研究

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-10 DOI: 10.1080/21691401.2025.2462333

Vino Udappusamy, Rajan Thinagaran, Vijayakumar Mayakrishnan, Janani Balakarthikeyan, Priya Kannappan, Sameer Al-Ghamdi, Naif Abdurhman Alrudian, Mohammed Saad Alqahtani, Khalid Albasheer, Chandrabose Sureka, Mahmoud H El-Bidawy, Nesreen Alsanousi, Sahar Gamil, Thiyagarajan Ramesh

{"title":"An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple-negative breast cancer cells with Psidium guajava peel-derived chitosan nanoparticles.","authors":"Vino Udappusamy, Rajan Thinagaran, Vijayakumar Mayakrishnan, Janani Balakarthikeyan, Priya Kannappan, Sameer Al-Ghamdi, Naif Abdurhman Alrudian, Mohammed Saad Alqahtani, Khalid Albasheer, Chandrabose Sureka, Mahmoud H El-Bidawy, Nesreen Alsanousi, Sahar Gamil, Thiyagarajan Ramesh","doi":"10.1080/21691401.2025.2462333","DOIUrl":"https://doi.org/10.1080/21691401.2025.2462333","url":null,"abstract":"Triple-negative breast cancer (TNBC) is a significant global health issue, with high mortality rates. The chemotherapeutic drugs currently used for TNBC have significant side effects, impacting both normal and cancer cells. In this study, we investigated a potential use of fruit peel extract of Psidium guajava (PGP) encapsulated with chitosan nanoparticles (CSNPs) to combat TNBC. The synthesized PGP-CSNPs were characterized using UV-vis spectroscopy, Fourier transform infra-red (FTIR) spectroscopy, TEM and GC-MS. The maximum loading capacity and encapsulation efficacy of PGP-CSNPs were found to be 72.5 ± 0.49% and 92.9 ± 0.10%, respectively. Furthermore, in vitro cytotoxicity was assessed, and the IC50 value for PGP-CSNPs was 50.13 µg/mL. It was observed that PGP-CSNPs could induce apoptosis in MDA-MB-231 cells in dose-dependent manner. Furthermore, molecular docking was performed for bioactive compounds retrieved from PGP-CSNPs against human tumour suppressor proteins Bcl2, and results showed that the PGP-CSNPs had lower binding energy than cisplatin. This suggests that, the synthesized PGP-CSNPs have the potential to serve as a therapeutic agent for tackling TNBC. However, to validate its efficacy in human therapy, furthermore pre-clinical and clinical procedures should be examined, as this is an ongoing and significant step towards developing an effective and safe anticancer drug.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"43-55"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Correction. 更正。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-14 DOI: 10.1080/21691401.2025.2478352

引用次数: 0