Artificial Cells, Nanomedicine, and Biotechnology最新文献

{"title":"Correction.","authors":"","doi":"10.1080/21691401.2026.2635327","DOIUrl":"https://doi.org/10.1080/21691401.2026.2635327","url":null,"abstract":"","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"192"},"PeriodicalIF":4.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147289050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of cell-derived vesicles from eukaryotic and prokaryotic origins for the delivery of biomolecules.","authors":"Jan Atienza-Garriga, Luke Smithers, Crystal Cooper, Alice Vrielink, Neus Ferrer-Miralles","doi":"10.1080/21691401.2025.2599072","DOIUrl":"https://doi.org/10.1080/21691401.2025.2599072","url":null,"abstract":"<p><p>Cell membrane-derived vesicles play essential roles in intercellular communication, material transport, and waste disposal. Despite their biomedical and industrial potential, isolating extracellular vesicles from natural sources remains technically challenging, limiting purification efficiency and scalability. This study introduces cell membrane extrusion as an alternative approach to optimize the production of cell membrane-derived vesicles (CSMs), from eukaryotic and prokaryotic cells. CSMs, generated from HeLa and SH-SY5Y cells exhibited a distinctive cup-shaped morphology and sizes of 151.36 ± 72.36 nm, and 416.86 ± 108.49 nm at 20 °C by DLS respectively, showing remarkable thermal stability at 4-70 °C range. Furthermore, loaded vesicles interacted with mammalian cells and achieved successful cargo internalization. CSMs were also produced from <i>E. coli</i> membranes, forming unilamellar vesicles of approximately 100 nm, as observed by Cryo-TEM. These vesicles displayed an inverse correlation between vesicle size and thermal stability and efficient cargo incorporation detected in 85% ± 3% of CSMs. However, under tested conditions, no interaction with prokaryotic cells occurred, and consequently, no delivery of the loaded molecule was observed. Overall, thesefindings highlight the potential of generating cell membrane-derived nanovesicles through extrusion, offering a promising strategy to mimic extracellular vesicles for innovative biomedical and industrial applications, including targeted drug delivery system.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"1-18"},"PeriodicalIF":4.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanism of Gei Herba against type 2 diabetes mellitus: an integration of gut microbiota and network pharmacology analysis.","authors":"Xi-Mei Zhang, Hao-Ming Zhou, Wen-Xiao Wang, Yong-Gang Wu, Yi-Xin Zhang, Shi-Jun Yue","doi":"10.1080/21691401.2026.2618968","DOIUrl":"10.1080/21691401.2026.2618968","url":null,"abstract":"<p><p>The present study aimed to dissect the underlying mechanism of the Gei Herba (LBZ) in the treatment of type 2 diabetes mellitus (T2DM) based on the gut microbiota and network pharmacology strategies. Thirty-one compounds from LBZ were screened and 187 corresponding targets were identified through the database and literature screening. Multiple disease-associated gene and target databases were used to screen and obtain 726 T2DM-related targets. The gutMGene v1.0 database was used for searching metabolites and targets of gut microbiota. 168 overlapping targets of LBZ, T2DM and the gut microbiota were matched and used to build a protein-protein interaction network and perform enrichment analysis. By bioinformatics analysis of microbiota-pathway-target-compound network, the PI3K-Akt signalling pathway was identified, and potential gut probiotics, such as <i>Eubacterium limosum</i> and <i>Lactobacillus paracasei</i>, were found. Subsequently, the core active compounds in LBZ bind to the core targets were verified by molecular docking, all of which exhibited good binding affinity. Finally, four compounds, eleutheroside A, gallic acid, kaempferol, and protocatechuic acid, were found to conform to Lipinski's rule and be non-toxic, showing great potential in the treatment of T2DM. These findings suggest the potential mechanism of LBZ in the treatment of T2DM and the core compounds, such as eleutheroside A may be the promising candidates for T2DM treatment.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"119-131"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148148306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-guided data-driven kinetic modelling carbon quantum dot-enabled pH-responsive CMC/CeO₂ nanocarriers for quercetin delivery and <i>in vitro</i> evaluation in lung cancer cells.","authors":"Nikoo Baghal Darbandi, Zeinab Rostami Dehka, Mehrab Pourmadadi, Salar Mohammadi Shabestari, Elham Hatami Monfared","doi":"10.1080/21691401.2026.2702868","DOIUrl":"https://doi.org/10.1080/21691401.2026.2702868","url":null,"abstract":"<p><p>This work reports the development and systematic evaluation of a carboxymethyl cellulose (CMC)-based nanocarrier system co-loaded with cerium oxide (CeO₂) and carbon quantum dots (CQDs) for pH-responsive delivery of quercetin (QC) and <i>in vitro</i> evaluation in lung cancer cells. The nanocarriers were prepared using a water-in-oil-in-water (W/O/W) double emulsion approach, yielding spherical particles with an average size of approximately 134 nm and a high positive surface charge (+66 mV), indicative of favourable colloidal stability. FESEM analysis confirmed a uniform morphology and compact internal structure. The incorporation of CeO₂ appears to reinforce the polymer matrix, contributing to improved drug encapsulation. The optimized formulation exhibited high encapsulation efficiency (88%) and drug loading capacity (47%), outperforming CeO₂-free systems. <i>In vitro</i> release studies demonstrated a clear pH-dependent biphasic behaviour, with significantly faster release under pH 5.4 compared to physiological pH (7.4), reaching 98% and 58% after 96 h, respectively. Drug release followed the Higuchi model, suggesting diffusion-controlled kinetics, while the Korsmeyer-Peppas model indicated a non-Fickian mechanism. An AI-guided nonlinear modelling workflow was used to extract interpretable kinetic descriptors directly from experimental release data. Biological evaluation revealed enhanced anticancer activity against A549 cells, with viability reduced to 49.1%, while maintaining high biocompatibility towards L929 cells.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"337-357"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and multi-layered experimental validation of feature genes related to butyrate metabolism and oxidative stress in sepsis.","authors":"Qinglu Fan, Renwei Luo, Lingxia Qin, Zhihao Nie, Qingyun Chen, Shujian Wei, Lingxuan Dan, Guang Li, Genhua Mu, Songping Xie","doi":"10.1080/21691401.2026.2658990","DOIUrl":"10.1080/21691401.2026.2658990","url":null,"abstract":"<p><p>The mechanisms underlying sepsis remain poorly understood and specific therapeutic options remain lacking. Accumulating evidence suggests that butyrate metabolism and oxidative stress (OS) are involved in the pathogenesis of sepsis. This study aims to elucidate molecular characteristics of butyrate metabolism and OS-related genes in sepsis and find potential therapeutic targets. Using sepsis-related datasets, 876 differential expression genes (DEGs) were screened and intersected with butyrate metabolism and OS-related genes to yield 8 differentially expressed butyrate metabolism and OS-related genes (DEBORGs). Machine learning algorithms finally identified 3 feature genes (ALDH1A1, CYP1B1, and GADD45A) with favourable diagnostic values for sepsis. CIBERSORT immune infiltration analysis demonstrated strong associations between feature genes and the infiltration proportions of various immune cell types, such as monocyte, macrophage, and neutrophil. Cellular experiment and prospectively collected clinical samples were utilized to validate the expression levels and diagnostic efficacy of feature genes. The scRNA-seq analysis indicated that monocyte plays a crucial role in mediating the effect of feature genes on sepsis. This study elucidates that ALDH1A1, CYP1B1, and GADD45A are feature genes linked to butyrate metabolism and OS in sepsis, with monocytes being the primary cells involved, providing novel insights into the sepsis pathogenesis and potential therapeutic targets.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"332-345"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics integrative bioinformatics analysis of gene expression characteristics and molecular mechanisms in preeclampsia placental tissue.","authors":"Ni Li, Lin Tian, Haiyun Wei","doi":"10.1080/21691401.2026.2697688","DOIUrl":"https://doi.org/10.1080/21691401.2026.2697688","url":null,"abstract":"<p><p>Preeclampsia (PE) is a severe pregnancy-specific complication characterized by new-onset hypertension and proteinuria after 20 weeks of gestation, which can cause multi-organ damage and life-threatening outcomes for both mothers and foetuses. Its pathogenesis remains incompletely elucidated, with placental dysfunction widely recognized as a core pathogenic factor. This study integrated multiple placental transcriptome and single-cell sequencing datasets from the Gene Expression Omnibus (GEO) database, employing a multi-dimensional bioinformatics approach - including differential expression analysis, Weighted Gene Co-expression Network Analysis (WGCNA), machine learning, molecular subtype clustering, single-cell resolution analysis, and intercellular communication analysis - to systematically identify PE-related key genes, construct a diagnostic model, define molecular subtypes, and explore potential molecular mechanisms. Results showed 10 differentially expressed genes (DEGs) were identified in PE placental tissues; WGCNA pinpointed the turquoise module as the core PE-associated module. Further screening using 11 machine learning algorithms identified 9 feature genes with high diagnostic value (DDR1, DIO2, FSTL3, HK2, HTRA4, LEP, SERPINA3, TMEM45A, TREM1). A diagnostic model built with the 'Stepglm[forward]' algorithm exhibited excellent performance in both training and validation sets (average AUC = 0.865). Molecular subtype analysis classified PE samples into two subtypes (C1, C2) with significantly distinct immune infiltration profiles, where the C1 subtype showed higher immune cell infiltration. Single-cell analysis identified 11 cell types in PE placental tissue and highlighted TMEM45A as a key DEG. Intercellular communication analysis revealed the VEGF signalling pathway as the core driver of abnormal cellular crosstalk in PE, primarily mediating signal transduction between villous cytotrophoblast cells (VCT), extravillous trophoblast cells (EVT), and endothelial cells. Hypoxia scoring analysis demonstrated significantly higher hypoxia levels in the PE group compared to normal controls, with TMEM45A expression positively correlated with hypoxia scores. This study provides novel insights into the molecular pathogenesis of PE and offers potential biomarkers and a theoretical basis for its early diagnosis and targeted therapy.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"358-373"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Statement: Down-regulation of long non-coding RNA ITGB2-AS1 inhibits osteosarcoma proliferation and metastasis by repressing Wnt/β-catenin signalling and predicts favourable prognosis.","authors":"","doi":"10.1080/21691401.2026.2708515","DOIUrl":"https://doi.org/10.1080/21691401.2026.2708515","url":null,"abstract":"","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"394"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148598115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Statement: Long non-coding RNA NEAT1 promotes steatosis via enhancement of estrogen receptor alpha-mediated AQP7 expression in HepG2 cells.","authors":"","doi":"10.1080/21691401.2026.2708500","DOIUrl":"https://doi.org/10.1080/21691401.2026.2708500","url":null,"abstract":"","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"392"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148598135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Statement: Silencing long non-coding RNA SNHG6 restrains proliferation, migration and invasion of Wilms' tumour cell lines by regulating miR-15a.","authors":"","doi":"10.1080/21691401.2026.2708408","DOIUrl":"https://doi.org/10.1080/21691401.2026.2708408","url":null,"abstract":"","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"389"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148598180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and antibiofilm evaluation of biogenic ternary CuO-AgO-MgO nanocomposite against clinical isolates of carbapenem-resistant <i>Klebsiella pneumoniae</i>.","authors":"Mohammed S Abdulrahman, Nasir A Ibrahim, Fathy M Elkady, Amr H Hashem, Faisal Alsenani, Nosiba S Basher, Faisal H Altemani, Abdel-Aty E Alfeky, Ayman Salama, Adnan Alharbi, Sulaiman A Alsalamah, Fahd A Nasr, Mohammed Aufy, Ebrahim Saied","doi":"10.1080/21691401.2026.2665582","DOIUrl":"https://doi.org/10.1080/21691401.2026.2665582","url":null,"abstract":"<p><p>Escalation of antimicrobial resistance amongst <i>Klebsiella pneumoniae</i> (<i>K. pneumoniae</i>) represents a remarkable worldwide health concern. This study reports the green biosynthesis of copper, silver and magnesium oxide trimetallic nanocomposites (CuO-AgO-MgO TNCs) using banana peel extract as a safe and sustainable reducing and stabilizing agent and emphasizes its inhibitory activity against carbapenem-resistant <i>K. pneumoniae</i> (CRKP) strains. The TNCs were characterized by ultraviolet-visible (UV-vis) spectroscopy, Fourier transform infra-red (FTIR) spectroscopy, X-ray diffraction (XRD) analysis, transmission electron microscope (TEM), scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis, confirming their crystalline structure (average size 125 nm) and elemental composition (Cu, Ag, Mg, O). Cytotoxicity assessment using WI-38 normal cells showed minimal toxic effect with an IC₅₀ of 193.49 ± 2.85 µg/mL. Antibacterial activity was evaluated against genotypically diverse CRKP strains. Well diffusion assay (WDA) showed inhibition zones of 18-38 mm, while the broth microdilution assay provided minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values ranging from 8 to 1024 µg/mL and 128 to 2048 µg/mL, respectively. Time-kill assays indicated rapid bacterial growth reduction within 2 h, and antibiofilm assays showed 25-91% inhibition of biofilm formation. The image provided by SEM revealed pronounced morphological alterations in treated bacterial cells. Our findings exhibited biogenic CuO-AgO-MgO TNCs with potent antibacterial and antibiofilm activities against CRKP while showing negligible toxicity towards normal cells, highlighting their potential as a safe and effective alternative antimicrobial strategy.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"223-247"},"PeriodicalIF":4.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}