Artificial Cells, Nanomedicine, and Biotechnology最新文献

Correction. 修正。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-07 DOI: 10.1080/21691401.2025.2476906

引用次数: 0

Statement of Retraction: Myricetin nanoliposomes induced SIRT3-mediated glycolytic metabolism leading to glioblastoma cell death. 撤回声明：杨梅素纳米脂质体诱导sirt3介导的糖酵解代谢导致胶质母细胞瘤细胞死亡。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-27 DOI: 10.1080/21691401.2025.2465942

引用次数: 0

Purinergic ecto-enzymes in human and ovine aortic valves: indicators of bacterial nanocellulose scaffold cellularization. 人和羊主动脉瓣的嘌呤能外泌酶：细菌纳米纤维素支架细胞化的指标。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-12 DOI: 10.1080/21691401.2025.2502033

Barbara Kutryb-Zając, Ada Kawecka, Gabriela Harasim, Michał Bieńkowski, Klaudia Stawarska, Krzysztof Urbanowicz, Ryszard T Smoleński, Maciej M Kowalik, Magdalena Kołaczkowska, Piotr Siondalski

{"title":"Purinergic ecto-enzymes in human and ovine aortic valves: indicators of bacterial nanocellulose scaffold cellularization.","authors":"Barbara Kutryb-Zając, Ada Kawecka, Gabriela Harasim, Michał Bieńkowski, Klaudia Stawarska, Krzysztof Urbanowicz, Ryszard T Smoleński, Maciej M Kowalik, Magdalena Kołaczkowska, Piotr Siondalski","doi":"10.1080/21691401.2025.2502033","DOIUrl":"https://doi.org/10.1080/21691401.2025.2502033","url":null,"abstract":"Purinergic signalling pathways play a vital role in the biological functions of the aortic valve (AV) through nucleotide and adenosine-dependent receptor effects. This study focused on characterizing a side-specific purinergic cascade in human non-stenotic and stenotic AVs, ovine native AVs and a novel bacterial nanocellulose (BNC) bio-prosthesis in an ovine model. Human stenotic AVs were collected during replacement surgeries, while non-stenotic AVs came from heart transplant patients. Ovine native AVs were sourced from domestic sheep, and the BNC prosthesis was implanted in the ovine aorta for six months, with hemodynamic monitoring throughout. Biochemical assessments revealed a beneficial ecto-enzyme pattern in non-stenotic and native AVs, contrasting with a detrimental pattern in stenotic valves. The BNC prosthesis demonstrated significantly lower nucleotide conversion activities than native valves and displayed increased peripheral blood mononuclear cell adhesion on its aortic surface. These findings suggest that nucleotide-converting ecto-enzymes could serve as markers for the biological activity of AV prostheses, highlighting the need for further studies to enhance the cellularization of BNC prostheses, potentially through adenosine-releasing scaffold modifications.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"219-230"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multimodal attention fusion deep self-reconstruction presentation model for Alzheimer's disease diagnosis and biomarker identification. 多模态注意力融合深度自我重建呈现模型用于阿尔茨海默病诊断和生物标志物识别。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-23 DOI: 10.1080/21691401.2025.2506591

Shan Huang, Yixin Liu, Jingyu Zhang, Yiming Wang

{"title":"Multimodal attention fusion deep self-reconstruction presentation model for Alzheimer's disease diagnosis and biomarker identification.","authors":"Shan Huang, Yixin Liu, Jingyu Zhang, Yiming Wang","doi":"10.1080/21691401.2025.2506591","DOIUrl":"https://doi.org/10.1080/21691401.2025.2506591","url":null,"abstract":"The unknown pathogenic mechanisms of Alzheimer's disease (AD) make treatment challenging. Neuroimaging genetics offers a method for identifying disease biomarkers for early diagnosis, but traditional approaches struggle with complex non-linear, multimodal and multi-expression data. However, traditional association analysis methods face challenges in handling nonlinear, multimodal and multi-expression data. Therefore, a multimodal attention fusion deep self-restructuring presentation (MAFDSRP) model is proposed to solve the above problem. First, multimodal brain imaging data are processed through a novel histogram-matching multiple attention mechanisms to dynamically adjust the weight of each input brain image data. Simultaneous, the genetic data are preprocessed to remove low-quality samples. Subsequently, the genetic data and fused neuroimaging data are separately input into the self-reconstruction network to learn the nonlinear relationships and perform subspace clustering at the top layer of the network. Finally, the learned genetic data and fused neuroimaging data are analysed through expression association analysis to identify AD-related biomarkers. The identified biomarkers underwent systematic multi-level analysis, revealing biomarker roles at molecular, tissue and functional levels, highlighting processes like inflammation, lipid metabolism, memory and emotional processing linked to AD. The experimental results show that MAFDSRP achieved 0.58 in association analysis, demonstrating its great potential in accurately identifying AD-related biomarkers.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"231-243"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of macrophage-associated diagnostic biomarkers and molecular subtypes in gestational diabetes mellitus based on machine learning. 基于机器学习的妊娠糖尿病巨噬细胞相关诊断生物标志物和分子亚型鉴定

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-06 DOI: 10.1080/21691401.2025.2513893

Kai Wei, Liyun Yuan, Yongsheng Ge, Han Yu, Guoping Zhao, Guoqing Zhang, Guohua Liu

{"title":"Identification of macrophage-associated diagnostic biomarkers and molecular subtypes in gestational diabetes mellitus based on machine learning.","authors":"Kai Wei, Liyun Yuan, Yongsheng Ge, Han Yu, Guoping Zhao, Guoqing Zhang, Guohua Liu","doi":"10.1080/21691401.2025.2513893","DOIUrl":"https://doi.org/10.1080/21691401.2025.2513893","url":null,"abstract":"Gestational diabetes mellitus (GDM) is a common metabolic disorder during pregnancy, involving multiple immune and inflammatory factors. Macrophages play a crucial role in its development. This study integrated scRNA-seq and RNA-seq data to explore macrophage-related diagnostic genes and GDM subtypes. For scRNA-seq data, cell clusters were annotated using the SingleR package and validated with marker gene expression profiles, while hdWGCNA analysis identified three gene modules related to macrophages. A diagnostic model for GDM derived from endothelial cell transcriptomes was constructed by employing a variety of machine learning ensemble algorithms, achieving an AUC of 0.887. The model identified five differentially expressed genes (ZEB2, MALAT1, HEBP1, AHSA1, and TTC3) as potential diagnostic biomarkers. The CB-DSNMF algorithm was proposed to identify two distinct GDM subtypes from RNA-seq data, revealing significant differences in biological behaviours. This algorithm outperformed other baselines in multiple clustering metrics. Mendelian randomisation analysis identified ZEB2 as a gene causally related to GDM risk. A transcription factor (TF)-gene regulatory network was constructed for these genes using the ENCODE database. The study highlights the importance of macrophages in GDM, provides a high-precision diagnostic model, and offers new insights into personalised treatment strategies, contributing to a better understanding of GDM pathophysiology.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"20-33"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-04 DOI: 10.1080/21691401.2025.2473244

引用次数: 0

Human tendon stem/progenitor cell-derived extracellular vesicle production promoted by dynamic culture. 动态培养促进人肌腱干/祖细胞来源的细胞外囊泡生成。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/21691401.2025.2475099

Marta Clerici, Maria Camilla Ciardulli, Erwin Pavel Lamparelli, Joseph Lovecchio, Emanuele Giordano, Tina P Dale, Nicholas R Forsyth, Nicola Maffulli, Giovanna Della Porta

{"title":"Human tendon stem/progenitor cell-derived extracellular vesicle production promoted by dynamic culture.","authors":"Marta Clerici, Maria Camilla Ciardulli, Erwin Pavel Lamparelli, Joseph Lovecchio, Emanuele Giordano, Tina P Dale, Nicholas R Forsyth, Nicola Maffulli, Giovanna Della Porta","doi":"10.1080/21691401.2025.2475099","DOIUrl":"10.1080/21691401.2025.2475099","url":null,"abstract":"Tendon injuries significantly impact quality of life, prompting the exploration of innovative solutions beyond conventional surgery. Extracellular Vesicles (EVs) have emerged as a promising strategy to enhance tendon regeneration. In this study, human Tendon Stem/Progenitor Cells (TSPCs) were isolated from surgical biopsies and cultured in a Growth-Differentiation Factor-5-supplemented medium to promote tenogenic differentiation under static and dynamic conditions using a custom-made perfusion bioreactor. Once at 80% confluence, cells were transitioned to a serum-free medium for conditioned media collection. Ultracentrifugation revealed the presence of vesicles with a 106 particles/mL concentration and sub-200nm diameter size. Dynamic culture yielded a 3-fold increase in EV protein content compared to static culture, as confirmed by Western-blot analysis. Differences in surface marker expression were also shown by flow cytometric analysis. Data suggest that we efficiently developed a protocol for extracting EVs from human TSPCs, particularly under dynamic conditions. This approach enhances EV protein content, offering potential therapeutic benefits for tendon regeneration. However, further research is needed to fully understand the role of EVs in tendon regeneration.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"1-16"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The integration of metabolites from Forsythia suspensa and gut microbiota ameliorates drug-induced liver injury: network pharmacology and molecular docking studies. 连翘代谢物与肠道微生物群的整合改善药物性肝损伤：网络药理学和分子对接研究。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-07 DOI: 10.1080/21691401.2025.2475088

Yanni Wang, Xiangxiang Peng, Bingjie Qian, Libo Wang, Jiabing Wang

{"title":"The integration of metabolites from Forsythia suspensa and gut microbiota ameliorates drug-induced liver injury: network pharmacology and molecular docking studies.","authors":"Yanni Wang, Xiangxiang Peng, Bingjie Qian, Libo Wang, Jiabing Wang","doi":"10.1080/21691401.2025.2475088","DOIUrl":"10.1080/21691401.2025.2475088","url":null,"abstract":"This study integrates metabolites from Forsythia suspensa (FS) and gut microbiota GM to assess combined therapeutic efficacy against drug-induced liver injury (DILI) using network pharmacology and molecular docking. Metabolites of FS and GM were retrieved from the NPASS and gutMGene databases, respectively. Relevant targets for metabolites and DILI-related targets were identified through public databases. The PPI network and KEGG pathway analysis were employed to identify hub targets and key signalling pathways. Furthermore, we performed a molecular docking assay on the active metabolites and targets to verify the network pharmacological concept. The physicochemical properties and toxicity of identified key metabolites were assessed using in silico platforms. 19 final targets were recognized as key proteins responsible for the alleviation of DILI by FS and GM metabolites, with ESR1 emerging as a central target in the PPI network. The estrogen signalling pathway, particularly involving ESR1, ESR2 and JUN genes, was identified as a key mediator in the therapeutic effects. Four GM metabolites (baicalein, luteolin, lunularin and 2,3-bis(3,4-dihydroxybenzyl)butyrolactone) and two FS metabolites (pinoresinol and isolariciresinol) were identified as non-toxic, promising candidates. In conclusion, metabolites from FS and GM may exert a potent synergistic effect on DILI through modulation of the estrogen signalling pathway.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"105-121"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanistic action of mogroside V in the alleviation of oxidative aging. 苦参苷V在延缓氧化老化中的作用机制。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-11 DOI: 10.1080/21691401.2025.2486752

Qiannan Wang, Xinyue Mao, Yulan Li, Gang Mo, Dayu Li, Deping Cao, Gen Chen

{"title":"The mechanistic action of mogroside V in the alleviation of oxidative aging.","authors":"Qiannan Wang, Xinyue Mao, Yulan Li, Gang Mo, Dayu Li, Deping Cao, Gen Chen","doi":"10.1080/21691401.2025.2486752","DOIUrl":"https://doi.org/10.1080/21691401.2025.2486752","url":null,"abstract":"Introduce: Diseases related to oxidative ageing are becoming increasingly evident in younger individuals. In this study, we investigated the mechanisms underlying the actions of mogroside V when used to treat anti-oxidative ageing.Methods: We used D-galactose-induced LO2 cells and C57BL/6J mice as models to investigate the molecular mechanisms of mogroside V (MV) for the treatment of oxidative ageing. Network pharmacology was used to predict the targets of MV for the treatment of oxidative ageing.Results: By down-regulating the EGFR/p38/JNK pathway, MV significantly inhibited oxidative ageing and apoptosis in cells, reduced the levels of SA-β-galactosidase. In mice, compared with the model group, MV treatment (100 mg/kg·d) reduced MDA levels and significantly increased the levels of GSH and SOD; furthermore, the size and structure of the liver leaflet and glomeruli was arranged in a regular manner; the small intestine glands had decreased in size. Moreover, the expression levels of Ptp1b mRNA had increased significantly while the levels of c-Jun mRNA and protein were significantly reduced. MV also increased the proportion of beneficial bacteria in the small intestine, including Bacteroidales and Lactobacillaceae.Conclusion: Our analyses revealed that MV can significantly reduce oxidative ageing caused by the accumulation of D-galactose.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"166-180"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial capping agents on silver nanoparticles made via green method using natural products from banana plant waste. 利用香蕉植物废料中的天然产物，通过绿色方法制备纳米银抗菌封盖剂。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-07 DOI: 10.1080/21691401.2025.2462335

Jimmy K Kabeya, Nadège K Ngombe, Paulin K Mutwale, Justin B Safari, Gauta Gold Matlou, Rui W M Krause, Christian I Nkanga

{"title":"Antimicrobial capping agents on silver nanoparticles made via green method using natural products from banana plant waste.","authors":"Jimmy K Kabeya, Nadège K Ngombe, Paulin K Mutwale, Justin B Safari, Gauta Gold Matlou, Rui W M Krause, Christian I Nkanga","doi":"10.1080/21691401.2025.2462335","DOIUrl":"10.1080/21691401.2025.2462335","url":null,"abstract":"Herein, we investigated the phytochemical composition and antibacterial activities of the organic layers from biosynthesized silver nanoparticles (AgNPs). AgNPs were synthesized using Musa paradisiaca and Musa sapientum extracts. UV-vis absorption in the 400-450 nm range indicated surface plasmonic resonance peak of AgNPs. Samples analyses using dynamic light scattering and transmission electron microscopy revealed the presence of particles within nanometric ranges, with sizes of 30-140 nm and 8-40 nm, respectively. Fourier transform infrared (FTIR) unveiled the presence of several organic functional groups on the surface of AgNPs, indicating the presence of phytochemicals from plant extracts. Thin layer chromatography (TLC) of the phytochemicals (capping agents) from AgNPs identified multiple groups of secondary metabolites. These phytochemical capping agents exhibited antibacterial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, with minimum inhibitory concentrations ranging from 62.5 to 1000 µg/mL. Regardless of the bacterial species or plant parts (leaves or pseudo-stems), capping agents from M. sapientum nanoparticles displayed significantly enhanced antibacterial effectiveness compared to all other samples, including the raw plant extracts and biosynthesized capped and uncapped AgNPs. These results suggest the presence of antimicrobial phytochemicals on biosynthesized AgNPs, highlighting the promise of green nanoparticle synthesis as a valuable approach in bioprospecting antimicrobial agents.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"29-42"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0