Artificial Cells, Nanomedicine, and Biotechnology最新文献_第2页

Prefrontal cortex-extracellular vesicle RNAs coupling in alcohol use disorder: towards non-invasive biomarkers and targeted therapeutics. 前额叶皮层-细胞外囊泡rna在酒精使用障碍中的偶联：面向非侵入性生物标志物和靶向治疗

IF 5.6 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-01-28 DOI: 10.1080/21691401.2026.2617703

Jie-Quan Wang, Qing-Rong Xia, Xuan Zhou, Chun-Yu Yan, Wen-Xiu Hong, Yi-Yun Deng, Feng Shan, Ya-Ru Liu, Jun Liang

{"title":"Prefrontal cortex-extracellular vesicle RNAs coupling in alcohol use disorder: towards non-invasive biomarkers and targeted therapeutics.","authors":"Jie-Quan Wang, Qing-Rong Xia, Xuan Zhou, Chun-Yu Yan, Wen-Xiu Hong, Yi-Yun Deng, Feng Shan, Ya-Ru Liu, Jun Liang","doi":"10.1080/21691401.2026.2617703","DOIUrl":"10.1080/21691401.2026.2617703","url":null,"abstract":"Alcohol use disorder (AUD) poses a substantial global health burden, with its molecular mechanism remaining elusive. This study integrated datasets (GSE253155, GSE180722, GSE182173 and GSE176122) and clinical specimens to identify non-invasive biomarkers and therapeutic candidates for AUD. Transcriptomic analysis of post-mortem prefrontal cortex identified 34 differentially expressed genes (DEGs), significantly enriched in TNF/IL-17 signalling, ribosome, cytokine-cytokine receptor interaction and endoplasmic reticulum protein processing. Among multiple machine learning algorithms, the support vector machine demonstrated superior classification performance, prioritizing MDK, FAM225B and SERPINA3 as critical biomarkers. These were incorporated into a highly predictive diagnostic nomogram. Intersection with plasma extracellular vesicle (EV) RNAs showed that all three biomarkers were detectable and upregulated in AUD patients versus controls, which was confirmed by quantitative real-time PCR (qRT-PCR) in a clinical validation cohort. SERPINA3 exhibited a particularly robust association with AUD, maintaining significance after adjustment for confounders and suggesting direct effects via mediation analysis. Cross-species validation confirmed a conserved dysregulation of murine Serpina3n. Computational drug repurposing identified loratadine, doxepin, citalopram and imipramine as promising therapeutic candidates targeting SERPINA3. Collectively, this work delineated PFC signature genes, proposed a EVs-supported brain-periphery molecular crosstalk, and provided translatable biomarkers and repurposable candidates for AUD precision medicine.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"132-149"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148148333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel anti-rheumatic potential of Eucalrobusone C: inhibition of rheumatoid arthritis fibroblast-like synoviocytes and metabolic reprogramming. Eucalrobusone C新的抗风湿潜能：抑制类风湿关节炎成纤维细胞样滑膜细胞和代谢重编程。

IF 5.6 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-03-17 DOI: 10.1080/21691401.2026.2625669

Yaobin Zhu, Ting Chen, Xuebing Feng, Jiewei Luo, Jinshui Chen, Tianmin Wu

{"title":"Novel anti-rheumatic potential of Eucalrobusone C: inhibition of rheumatoid arthritis fibroblast-like synoviocytes and metabolic reprogramming.","authors":"Yaobin Zhu, Ting Chen, Xuebing Feng, Jiewei Luo, Jinshui Chen, Tianmin Wu","doi":"10.1080/21691401.2026.2625669","DOIUrl":"10.1080/21691401.2026.2625669","url":null,"abstract":"Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by synovial hyperplasia, inflammatory cell infiltration, and joint destruction. This study investigates the inhibitory effects and metabolic mechanisms of Eucalrobusone C (EC), a novel formyl-phloroglucinol meroterpenoid derivative isolated from Eucalyptus robusta, on Tumour Necrosis Factor-α (TNF-α)-induced rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs). EC was extracted and purified, with purity confirmed using 1H Nuclear Magnetic Resonance Spectrum (NMR) at 400 MHz. RA-FLSs were exposed to varying concentrations of EC, followed by comprehensive assessment including CCK8 assay for cell proliferation, flow cytometry for cell death, and Transwell assay for migration and invasion capacity. Metabolomic profiling employed Ultra-High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-Q-TOF MS), integrated with multivariate statistical analysis and bioinformatics tools to identify metabolic alterations. Results indicated that EC suppressed RA-FLS proliferation in a time- and concentration-dependent manner, significantly enhanced apoptosis, and inhibited cell migration and invasion. Metabolomics analysis detected 898 metabolites, with 112 upregulated and 67 downregulated in EC-treated groups compared to TNF-α-induced controls. Key differentially expressed metabolites were enriched in pathways including ABC transporters, neuroactive ligand-receptor interactions, protein digestion and absorption, and cAMP signalling. These findings suggest that EC exerts anti-rheumatic effects by modulating these metabolic pathways, offering potential as a therapeutic agent for RA management.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"232-244"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of phototherapeutic antibacterial activity of LED-illuminated curcumin nanomicelles against multidrug-resistant K. pneumoniae harbouring extended-spectrum β-Lactamase (ESBL) and carbapenemase resistance genes. led照明的姜黄素纳米胶束对含有广谱β-内酰胺酶（ESBL）和碳青霉烯酶耐药基因的多重耐药肺炎克雷伯菌光治疗抗菌活性的评价

IF 5.6 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-09-20 DOI: 10.1080/21691401.2026.2727297

Farhad Moradi, Roghayyeh Vakili-Ghartavol, Mohsen Ostovari, Maryam Akbari, Nahal Hadi

{"title":"Evaluation of phototherapeutic antibacterial activity of LED-illuminated curcumin nanomicelles against multidrug-resistant K. pneumoniae harbouring extended-spectrum β-Lactamase (ESBL) and carbapenemase resistance genes.","authors":"Farhad Moradi, Roghayyeh Vakili-Ghartavol, Mohsen Ostovari, Maryam Akbari, Nahal Hadi","doi":"10.1080/21691401.2026.2727297","DOIUrl":"https://doi.org/10.1080/21691401.2026.2727297","url":null,"abstract":"This study evaluated the antibacterial effect of photodynamic therapy (aPDT) using nanomicelle-encapsulated curcumin photosensitizer (PS) against drug-resistant clinical isolates of Klebsiella pneumoniae and K. pneumoniae ATCC 13883™. In this study, nanomicelle curcumin prepared by the thin film hydration method, with LED as the radiation source, was used for effective aPDT. The technique's effectiveness was analysed through membrane changes tracked by flow cytometry, bacterial structural alterations observed via FTIR, lipid peroxidation quantification assay, atomic force microscopy, and its performance against 17 clinical isolates resistant to cephalosporins and carbapenems was evaluated. Based on the results of aPDT, the minimum inhibitory concentration of the PS was 200 μg/mL for the standard strain and 125-1000 μg/mL for superbug clinical isolates. With an irradiation dose of 500 J/cm2, an irradiation time of 500 s, and a power density of 1000 mW/cm2 from an LED, bacterial growth was inhibited by a mean of 8 Log10 CFU/mL reduction, as determined by the culture method. Additionally, the results of the bacterial structural integrity assays (flow cytometry, FTIR, MDA levels, and AFM) for the standard strain provided convergent evidence with the culture results. This study demonstrates antibacterial activity of LED-illuminated curcumin nanomicelles against multidrug-resistant K. pneumoniae.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"819-837"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The unmasking of two-faced portrait in sorafenib-resistant surroundings via systems pharmacology concept: a brightness or a silhouette. 通过系统药理学概念揭示索拉非尼耐药环境中双面肖像：亮度或轮廓。

IF 5.6 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-04-19 DOI: 10.1080/21691401.2026.2655581

Ki-Kwang Oh, Goo-Hyun Kwon, Jung-A Eom, Kyeong Jin Lee, Dong Joon Kim, Ki-Tae Suk

{"title":"The unmasking of two-faced portrait in sorafenib-resistant surroundings via systems pharmacology concept: a brightness or a silhouette.","authors":"Ki-Kwang Oh, Goo-Hyun Kwon, Jung-A Eom, Kyeong Jin Lee, Dong Joon Kim, Ki-Tae Suk","doi":"10.1080/21691401.2026.2655581","DOIUrl":"10.1080/21691401.2026.2655581","url":null,"abstract":"This study aimed to identify novel key targets and mechanisms for repurposing strategies and mitigating sorafenib (SFB) resistance using the GEO transcriptomic dataset GSE94550 within a systems pharmacology framework. Potential counteracting molecules against SFB were retrieved from chemical repositories, followed by molecular docking tests (MDT), Kaplan-Meier survival analysis, and density functional theory (DFT) assessments to evaluate therapeutic potential. PPI networks were constructed using STRING and R to characterize the relationships between upregulated and downregulated genes. The most relevant signalling pathways associated with major targets were determined to elucidate the upstream regulatory mechanisms. Among the differentially expressed genes, APOB emerged as a pivotal regulator (log2FC ≥ +2 or ≤ -2), modulating fifteen genes, including eleven upregulated and four downregulated nodes. At stricter thresholds (log2FC ≥ +3 or ≤ -3 and ≥ +4 or ≤ -4), CD44 was identified as a key upregulated target. Its inhibition - particularly by verbacoside - was strongly associated with suppression of the ECM-receptor interaction pathway, suggesting a significant therapeutic axis. This study illuminates the molecular landscape of SFB-resistant environments through an integrative network approach and highlights verbacoside as a promising agent capable of attenuating SFB resistance, supporting its potential role in combination therapy.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"289-323"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer activity of fluoxetine Janus dendrimer against cancer cells. 氟西汀树突状分子对癌细胞的抗癌活性。

IF 5.6 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-05-21 DOI: 10.1080/21691401.2026.2672826

Marcos Martínez-García, Isabel Hernández-Rioja, Israel Barajas-Mendoza, Alberto Espinosa-Hernández, Teresa Ramirez-Apan, Elena Martínez-Klimova

{"title":"Anticancer activity of fluoxetine Janus dendrimer against cancer cells.","authors":"Marcos Martínez-García, Isabel Hernández-Rioja, Israel Barajas-Mendoza, Alberto Espinosa-Hernández, Teresa Ramirez-Apan, Elena Martínez-Klimova","doi":"10.1080/21691401.2026.2672826","DOIUrl":"10.1080/21691401.2026.2672826","url":null,"abstract":"Targeting the inflammation-related molecules with nonsteroidal anti-inflammatory drugs (NSAIDs) represents a promising approach for cancer prevention/therapy. We evaluated the in vitro anticancer effects of sulindac, ketoprofen, celecoxib and the antidepressant fluoxetine, both free and coupled with synthesized dendrons and dendrimers, on the proliferation and apoptosis of human MCF-7 (human mammary adenocarcinoma), SKLU-1 (human lung adenocarcinoma) and as a control the normal monkey kidney (COS-7) cell line. The antiproliferative activity and cytotoxicity of tested NSAIDs on MCF-7 and SKLU-1 cell lines were assessed by the sulforhodamine B (SRB) assay. The tested dendrons with NSAIDs showed activity against the tumour cell lines. Significant inhibition of the growth of cancer cells was observed for the Janus dendrimers with sulindac-celecoxib, which was selective against MCF-7; and Janus dendrimers with fluoxetine were highly cytotoxic. The fluoxetine-dendrimer could be a candidate for the development of new pharmacological strategies for the treatment and prevention of MCF-7 cancer.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"106-119"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147986295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the safety and efficacy of micronized encapsulated ferric pyrophosphate in patients with iron deficiency anaemia: a phase-IV open-label clinical study. 评价微胶囊化焦磷酸铁治疗缺铁性贫血的安全性和有效性：一项iv期开放标签临床研究

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-01-28 DOI: 10.1080/21691401.2026.2618967

Belal Almajali, Giriraja Kv, Gowthamarajan Kuppusamy, Md Zeyaullah, Nayudu Teja, Veera Venkata Satyanarana Reddy Karri, Mohamed Rahamathulla, Muhammad Ali Abdullah Almoyad, Khursheed Muzammil, Mohammed Muqtader Ahmed, Ismail Pasha

{"title":"Assessment of the safety and efficacy of micronized encapsulated ferric pyrophosphate in patients with iron deficiency anaemia: a phase-IV open-label clinical study.","authors":"Belal Almajali, Giriraja Kv, Gowthamarajan Kuppusamy, Md Zeyaullah, Nayudu Teja, Veera Venkata Satyanarana Reddy Karri, Mohamed Rahamathulla, Muhammad Ali Abdullah Almoyad, Khursheed Muzammil, Mohammed Muqtader Ahmed, Ismail Pasha","doi":"10.1080/21691401.2026.2618967","DOIUrl":"https://doi.org/10.1080/21691401.2026.2618967","url":null,"abstract":"An open-level, single-arm, phase-4 clinical trial was carried out to assess the safety and potential benefits of micronized coated ferric pyrophosphate (MEFP) in patients with iron deficiency anaemia (IDA). For 12 weeks, 60 patients between the ages of 18 and 60 with moderate IDA were randomly received MEFP by PO daily. The efficacy endpoints as haemoglobin levels, mean corpuscular haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), packed cell volume (PCV), red blood cell count (RBC), serum ferritin and transferrin saturation (%) were measured. Adverse event reports and physical examinations were performed as a measure of safety assessment. The results revealed that haemoglobin, MCV, MCHC, serum ferritin, transferrin saturation (%), PCV and RBC increased significantly from baseline. Fewer occurrences were observed in a few patients, and their adverse events were minimal. There was no adverse effect on liver or renal functions. Few minor improvements were noticed at the completion of the study. In conclusion, MEFP appears to be effective in IDA and well tolerated, with a favourable safety profile. MEFP is an effective, safe therapeutic alternative in IDA subjects for increasing haemoglobin concentration and iron stores along with improvement of symptoms related to anaemia.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"150-158"},"PeriodicalIF":4.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146096805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of the single-cell and transcriptomics uncovers the pathogenic network and potential biomarkers related to hypoxia, metabolic reprogramming and atherosclerosis in obstructive sleep apnoea. 单细胞和转录组学的综合分析揭示了阻塞性睡眠呼吸暂停中与缺氧、代谢重编程和动脉粥样硬化相关的致病网络和潜在生物标志物。

IF 5.6 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-10-01 DOI: 10.1080/21691401.2026.2730116

Huiqian Yang, Hongjie Du, Chunyan Zang, Jian Liu, Zheng Wang, Fenglei Xu, Hui Li, Xiaoming Li

{"title":"Comprehensive analysis of the single-cell and transcriptomics uncovers the pathogenic network and potential biomarkers related to hypoxia, metabolic reprogramming and atherosclerosis in obstructive sleep apnoea.","authors":"Huiqian Yang, Hongjie Du, Chunyan Zang, Jian Liu, Zheng Wang, Fenglei Xu, Hui Li, Xiaoming Li","doi":"10.1080/21691401.2026.2730116","DOIUrl":"https://doi.org/10.1080/21691401.2026.2730116","url":null,"abstract":"This study aimed to identify key biomarkers for obstructive sleep apnoea (OSA) by analysing RNA-seq (GSE75097: 6 control and 42 OSA) and single-cell RNA-seq (GSE214865: 11 control and 11 OSA) datasets. Differential expression analysis revealed 575 DEGs between OSA and control groups. Using weighted gene co-expression network analysis (WGCNA) combined with machine learning approaches (Random Forest, SVM-RFE and LASSO), we identified four hub genes (C17ORF91, HBEGF, PGBD2 and ZNF616) as candidate biomarkers, showing exploratory discriminatory performance (AUC > 0.7). Gene set enrichment analysis (GSEA) revealed that these genes are associated with pathways related to hypoxia response, atherosclerosis and metabolic reprogramming (NES as low as -2.049, adj. p < .05), as well as immune regulation (NES up to 1.932, adj. p < .05). Single-cell RNA sequencing analysis further demonstrated that cMonocytes play a central role in OSA progression, exhibiting the highest scores for hypoxia, metabolic dysfunction and atherosclerosis risk. An exploratory nomogram incorporating these hub genes showed favourable internal performance within the discovery cohort. These findings provide hypothesis-generating insights into the molecular features associated with OSA and identify these genes as candidate biomarkers that require independent external and functional validation. Further validation of these genes may provide a basis for evaluating their potential diagnostic and therapeutic relevance in OSA.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"838-862"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time RNA sensors for diabetes management: assessing the impact of endocrine disruptors on biosensing and point-of-care diagnostics. 用于糖尿病管理的实时RNA传感器：评估内分泌干扰物对生物传感和即时诊断的影响。

IF 5.6 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-06-11 DOI: 10.1080/21691401.2026.2679936

Prit Ashara, Muktesh Chandra, Praveen Kumar Sharma, Irfan Nurhidayat, Mohd Asif Shah

引用次数: 0

Characterization of genipin-crosslinked gelatin/PVA hydrogels and the chondroprotective influence of hydroxytyrosol: an In vitro study. 格尼平交联明胶/聚乙烯醇水凝胶的表征及羟基酪醇对软骨保护作用的体外研究。

IF 5.6 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-06-22 DOI: 10.1080/21691401.2026.2678704

Wafa Ali Batarfi, Mohd Heikal Mohd Yunus, Adila A Hamid, Manira Maarof, Rizal Abdul Rani

{"title":"Characterization of genipin-crosslinked gelatin/PVA hydrogels and the chondroprotective influence of hydroxytyrosol: an In vitro study.","authors":"Wafa Ali Batarfi, Mohd Heikal Mohd Yunus, Adila A Hamid, Manira Maarof, Rizal Abdul Rani","doi":"10.1080/21691401.2026.2678704","DOIUrl":"https://doi.org/10.1080/21691401.2026.2678704","url":null,"abstract":"Osteoarthritis (OA) presents a significant therapeutic challenge, necessitating innovative biomaterial strategies to reduce chronic inflammation and promote cartilage regeneration. This study developed a hybrid gelatine (GE)/polyvinyl alcohol (PVA) hydrogel crosslinked with genipin (GNP) to effectively deliver Hydroxytyrosol (HT). A 6% GE was incorporated with 3% and 5% PVA concentrations, followed by 0.1% (w/v) GNP. The physicochemical and biocompatibility properties were further evaluated. Physicochemical analysis identified the GPVA5%-GNP formulation as optimal, featuring an interconnected porous structure, optimal swelling ratio (640.43 ± 22.28%), improved hydrophilicity (44.50 ± 1.81°), controlled biodegradation, and enhanced compressive strength (96.88 ± 0.72%). Chemical tests confirmed thermal and structural stability, while biocompatibility assays showed over 95% cell viability. The therapeutic potential was evaluated using an IL-1β-induced inflammatory model in human chondrocytes. MTT assays revealed that HT at 12.5, 25, and 50 μM restored metabolic activity in a dose-dependent manner over 14 days, outperforming the 9 μM Glucosamine Sulphate positive control. The 50 μM HT dose yielded the best metabolic recovery, supported by Live/Dead imaging that showed increased viable cell density from Day 1 to Day 7. Overall, the GPVA5%-GNP hydrogel loaded with 50 μM HT offers a robust protective environment that reduces catabolic stress and promotes cell proliferation, making it a promising localized therapy for cartilage regeneration in OA.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"248-275"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A functional polymorphism in ADAMTS7 3'-UTR abrogates miR-654-5p-mediated ADAMTS7 expression suppression and increases the incidence, short-term outcome, and recurrence of large artery atherosclerotic stroke among southern Chinese population. ADAMTS7 3'-UTR的功能多态性消除了mir -654-5p介导的ADAMTS7表达抑制，增加了中国南方人群大动脉粥样硬化性卒中的发病率、短期预后和复发。

IF 5.6 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2026-12-01 Epub Date: 2026-05-25 DOI: 10.1080/21691401.2026.2670966

Linfa Chen, Shan Wei, Zishan Li, Yutian Zhang, Jiahao Li, Jinjin Yan, Chun Xiao, You Li

{"title":"A functional polymorphism in ADAMTS7 3'-UTR abrogates miR-654-5p-mediated ADAMTS7 expression suppression and increases the incidence, short-term outcome, and recurrence of large artery atherosclerotic stroke among southern Chinese population.","authors":"Linfa Chen, Shan Wei, Zishan Li, Yutian Zhang, Jiahao Li, Jinjin Yan, Chun Xiao, You Li","doi":"10.1080/21691401.2026.2670966","DOIUrl":"10.1080/21691401.2026.2670966","url":null,"abstract":"Background: A Disintegrin and Metalloproteinase with Thrombospondin motifs 7 (ADAMTS7) plays a critical role in atherosclerosis by degrading the extracellular matrix and modulating smooth muscle cell (SMC) proliferation, migration, and phenotypic transformation. This study investigated the association between the ADAMTS7 single-nucleotide polymorphism (SNP) rs1045130 and large artery atherosclerotic (LAA) stroke.Materials and methods: Genotyping analyses were conducted on two independent case-control cohorts comprising 1,027 LAA patients and 1,043 age-matched controls, followed by the quantification of ADAMTS7 expression and its downstream molecular effects.Results: ADAMTS7 was highly expressed in endothelial cells, SMCs, and macrophage-derived foam cells of vulnerable human aortic plaques. Furthermore, individuals carrying the rs1045130 A allele or GA/AA genotype had a significantly higher susceptibility to LAA stroke, poorer short-term outcomes, and an increased risk of one-month recurrence. Mechanistically, the G > A mutation disrupts the ADAMTS7-miR-654-5p interaction, leading to elevated ADAMTS7 expression and subsequent promotion of foamed vascular SMCs (VSMCs) proliferation and migration.Conclusions: The rs1045130 G > A variant is associated with increased LAA stroke incidence and worse prognosis. The mutation promotes the proliferation and migration of foamed VSMCs by disrupting the miR-654-5p/ADAMTS7 axis, potentially accounting for the higher disease risk and poorer prognosis observed in affected individuals.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"54 1","pages":"137-151"},"PeriodicalIF":5.6,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0