Artificial Cells, Nanomedicine, and Biotechnology最新文献_第2页

Green synthesis of silver nanoparticles for functional cotton fabrics: antimicrobial efficacy against multidrug-resistant bacteria and cytotoxicity evaluation. 功能棉织物用纳米银的绿色合成：对多重耐药细菌的抗菌效果及细胞毒性评价。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-01 DOI: 10.1080/21691401.2025.2485115

Sérgio Antunes Filho, Clara M Almeida, Maria Teresa Villela Romanos, Bianca Pizzorno Backx, Raquel Regina Bonelli

{"title":"Green synthesis of silver nanoparticles for functional cotton fabrics: antimicrobial efficacy against multidrug-resistant bacteria and cytotoxicity evaluation.","authors":"Sérgio Antunes Filho, Clara M Almeida, Maria Teresa Villela Romanos, Bianca Pizzorno Backx, Raquel Regina Bonelli","doi":"10.1080/21691401.2025.2485115","DOIUrl":"10.1080/21691401.2025.2485115","url":null,"abstract":"Bacterial infections associated with healthcare are a challenge on a global scale due to the high morbidity and mortality rates, especially those caused by multidrug-resistant isolates. Hospital textiles are abiotic surfaces that may serve as a means of disseminating and persisting microorganisms in hospitals, as microorganisms can remain viable on these surfaces for up to months. In this study, we employed a green synthesis approach utilizing guava leaf extract (Psidium guajava) to produce silver nanoparticles, which were then incorporated into a cotton fabric. Antimicrobial properties and the cytotoxicity of the functional textile were assessed. The finding indicated that the green synthesis method was efficient and resulted in a predominant population of nanoparticles with diameters ranging from 25 to 84 nm that were uniformly dispersed in the textile. The functional textile exhibited low toxicity and high antimicrobial efficiency, even against multidrug-resistant microorganisms of particular concern in hospital settings. Atomic force microscopy carried out evidenced invaginations in the cell wall of bacteria submitted to this textile, suggesting surface damage as an important mechanism of action silver nanoparticles incorporated.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"153-165"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the anticancer and synergistic effects of the combination of green synthesized silver nanoparticles and papaverine on breast cancer cell lines: focusing on the apoptosis pathway and microRNA regulation. 研究绿色合成纳米银与罂粟碱联合对乳腺癌细胞株的抗癌和协同作用：重点研究凋亡途径和microRNA调控。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-01 DOI: 10.1080/21691401.2025.2507372

Maryam Fekri Soufiabadi, Reza Haji Hosseini, Zolfaghar Lotfi

{"title":"Investigating the anticancer and synergistic effects of the combination of green synthesized silver nanoparticles and papaverine on breast cancer cell lines: focusing on the apoptosis pathway and microRNA regulation.","authors":"Maryam Fekri Soufiabadi, Reza Haji Hosseini, Zolfaghar Lotfi","doi":"10.1080/21691401.2025.2507372","DOIUrl":"https://doi.org/10.1080/21691401.2025.2507372","url":null,"abstract":"The study investigates the anticancer effects of green silver nanoparticles (Ag-NPs) synthesized from Viola cornuta extract combined with papaverine on breast cancer cells. Ag-NPs were characterized using various analytical techniques, confirming their presence with UV-vis spectroscopy showing a peak at 413 nm and an average size of 42 nm via field emission scanning electron microscopy (FE-SEM) analysis. The particles demonstrated a face-centred cubic structure, with energy-dispersive X-ray spectroscopy (EDX) confirming elemental composition. Additionally, the zeta potential measurement of -6.75 mV indicated favourable electrostatic repulsion between nanoparticles, thereby confirming their stability. Antioxidant activity was significant, with an EC50 value of 38.78 μg/mL. The combination treatment of Ag-NPs and papaverine exhibited synergistic effects, lowering IC50 values to 2.8 + 112.7 μg/mL for MCF-7 cells and 6.2 + 112 μg/mL for MDA-MB-231 cells, without toxicity to normal cells. Flow cytometry revealed G0/G1 phase inhibition and increased sub-G1 populations, indicating cell cycle arrest, alongside increased reactive oxygen species generation and apoptosis. Notably, the experimental group showed altered expression of oncogenic and tumour suppressor microRNAs and apoptotic genes (p < .0001), underscoring the potential of this nanoparticle-papaverine combination as an effective anticancer strategy against breast cancer treatment resistance.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"1-19"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-16 DOI: 10.1080/21691401.2025.2532221

引用次数: 0

Microvesicles and exosomes isolated from murine bone marrow-derived mesenchymal stromal cells primed with p38MAPK inhibitor differentially regulate hematopoietic stem cell function. 从小鼠骨髓间充质基质细胞中分离的微囊泡和外泌体对造血干细胞功能的调控存在差异。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/21691401.2025.2475095

Pallavi Budgude, Vaijayanti Kale, Anuradha Vaidya

{"title":"Microvesicles and exosomes isolated from murine bone marrow-derived mesenchymal stromal cells primed with p38MAPK inhibitor differentially regulate hematopoietic stem cell function.","authors":"Pallavi Budgude, Vaijayanti Kale, Anuradha Vaidya","doi":"10.1080/21691401.2025.2475095","DOIUrl":"10.1080/21691401.2025.2475095","url":null,"abstract":"The signaling mechanisms active within mesenchymal stromal cells (MSCs) influence the composition of microvesicles (MVs) and exosomes (Exos) secreted by them. Previously, we showed that priming MSCs with a p38 pharmacological inhibitor (pMSCs) rejuvenates them and improves their ability to promote ex vivo hematopoietic stem cell (HSC) expansion. This study examined whether pMSCs exerted HSC-supportive ability via MVs (pMVs) and Exos (pExos). Our findings demonstrate distinct regulation of HSC fate by pMVs and pExos. pMVs promoted the expansion of long-term HSCs (LT-HSCs), distinguished by their robust self-renewal capacity and superior engraftment ability. In contrast, pExos facilitated expansion of short-term HSCs (ST-HSCs) with high proliferative and differentiation potential. Infusing a combination of pMVs- and pExos-expanded HSCs as a composite graft resulted in significantly higher HSC engraftment, emphasizing the synergistic interaction between LT- and ST-HSC populations. Gene expression studies, functional and phenotypic experiments showed that pMVs regulate HSC quiescence via the Egr1/Cdkn1a axis, while pExos control HSC proliferation via the Nfya/Cdkn1a axis. These findings provide insights into the molecular mechanisms underlying the differential regulation of HSC function by pMVs and pExos. It also proposes a composite graft strategy of using pMVs and pExos as \"MSC-derived biologics\" for improving the HSC transplantation success.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"122-137"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple-negative breast cancer cells with Psidium guajava peel-derived chitosan nanoparticles. 瓜石榴皮衍生壳聚糖纳米颗粒对MDA-MB-231三阴性乳腺癌细胞的靶向细胞毒性研究

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-10 DOI: 10.1080/21691401.2025.2462333

Vino Udappusamy, Rajan Thinagaran, Vijayakumar Mayakrishnan, Janani Balakarthikeyan, Priya Kannappan, Sameer Al-Ghamdi, Naif Abdurhman Alrudian, Mohammed Saad Alqahtani, Khalid Albasheer, Chandrabose Sureka, Mahmoud H El-Bidawy, Nesreen Alsanousi, Sahar Gamil, Thiyagarajan Ramesh

{"title":"An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple-negative breast cancer cells with Psidium guajava peel-derived chitosan nanoparticles.","authors":"Vino Udappusamy, Rajan Thinagaran, Vijayakumar Mayakrishnan, Janani Balakarthikeyan, Priya Kannappan, Sameer Al-Ghamdi, Naif Abdurhman Alrudian, Mohammed Saad Alqahtani, Khalid Albasheer, Chandrabose Sureka, Mahmoud H El-Bidawy, Nesreen Alsanousi, Sahar Gamil, Thiyagarajan Ramesh","doi":"10.1080/21691401.2025.2462333","DOIUrl":"10.1080/21691401.2025.2462333","url":null,"abstract":"Triple-negative breast cancer (TNBC) is a significant global health issue, with high mortality rates. The chemotherapeutic drugs currently used for TNBC have significant side effects, impacting both normal and cancer cells. In this study, we investigated a potential use of fruit peel extract of Psidium guajava (PGP) encapsulated with chitosan nanoparticles (CSNPs) to combat TNBC. The synthesized PGP-CSNPs were characterized using UV-vis spectroscopy, Fourier transform infra-red (FTIR) spectroscopy, TEM and GC-MS. The maximum loading capacity and encapsulation efficacy of PGP-CSNPs were found to be 72.5 ± 0.49% and 92.9 ± 0.10%, respectively. Furthermore, in vitro cytotoxicity was assessed, and the IC50 value for PGP-CSNPs was 50.13 µg/mL. It was observed that PGP-CSNPs could induce apoptosis in MDA-MB-231 cells in dose-dependent manner. Furthermore, molecular docking was performed for bioactive compounds retrieved from PGP-CSNPs against human tumour suppressor proteins Bcl2, and results showed that the PGP-CSNPs had lower binding energy than cisplatin. This suggests that, the synthesized PGP-CSNPs have the potential to serve as a therapeutic agent for tackling TNBC. However, to validate its efficacy in human therapy, furthermore pre-clinical and clinical procedures should be examined, as this is an ongoing and significant step towards developing an effective and safe anticancer drug.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"43-55"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced cartilage repair using gelatin methacryloyl hydrogels combined with icariin and magnesium-doped bioactive glass. 用明胶甲基丙烯酰水凝胶联合淫羊藿苷和掺镁生物活性玻璃增强软骨修复。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-15 DOI: 10.1080/21691401.2025.2490677

Shiyao Liao, Kai Zhou, Yao Kang, Tingxiao Zhao, Yicheng Lin, Jun Lv, Danjie Zhu

{"title":"Enhanced cartilage repair using gelatin methacryloyl hydrogels combined with icariin and magnesium-doped bioactive glass.","authors":"Shiyao Liao, Kai Zhou, Yao Kang, Tingxiao Zhao, Yicheng Lin, Jun Lv, Danjie Zhu","doi":"10.1080/21691401.2025.2490677","DOIUrl":"https://doi.org/10.1080/21691401.2025.2490677","url":null,"abstract":"Cartilage repair remains challenging due to limited self-healing, poor biocompatibility, and insufficient mechanical properties of current materials. To overcome these issues, we developed a multifunctional composite hydrogel by integrating gelatine methacrylate (GelMA) with magnesium-doped bioactive glass (Mg-BG) and icariin (ICA). SEM analysis revealed that pure GelMA exhibited a highly porous yet loosely organized structure, whereas the addition of Mg-BG and ICA produced a denser, more interconnected porous network that enhances cell adhesion and nutrient diffusion. In vitro, the ICA/Mg-BG/GelMA hydrogel achieved a swelling ratio up to 430% and maintained cell viability above 80% over 5 days. Moreover, qRT-PCR and immunohistochemical analyses demonstrated that the composite hydrogel upregulated chondrogenic markers (SOX9, ACAN, and COL2A1) compared with GelMA alone. Specifically, it downregulates M1 pro-inflammatory markers (CCR7, iNOS, CD86) and upregulates M2 anti-inflammatory markers (ARG1, CD163, CD206), thereby creating a regenerative microenvironment. These results indicate that the synergistic combination of GelMA, Mg-BG, and ICA not only improves the scaffold's mechanical support but also enhances its biological functionality, offering a promising strategy for cartilage repair. Future studies will focus on in vivo validation to further assess its clinical potential.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"181-193"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of mitophagy-related biomarkers in human rheumatoid arthritis using machine learning models. 利用机器学习模型鉴定类风湿关节炎中与线粒体自噬相关的生物标志物。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-16 DOI: 10.1080/21691401.2025.2533361

Jiayi Chen, Zuhai Huang, Chengyu Qin, Zixiang Pang, Yuanming Chen

{"title":"Identification of mitophagy-related biomarkers in human rheumatoid arthritis using machine learning models.","authors":"Jiayi Chen, Zuhai Huang, Chengyu Qin, Zixiang Pang, Yuanming Chen","doi":"10.1080/21691401.2025.2533361","DOIUrl":"https://doi.org/10.1080/21691401.2025.2533361","url":null,"abstract":"Rheumatoid arthritis (RA) is a systemic immune-mediated disease characterized by synovitis and joint cartilage destruction. Although many studies have shown that mitophagy is crucial in the development of bone metabolism disorders, its exact function in rheumatoid arthritis (RA) is still not well understood. This study analysed the GSE77298 dataset from the Gene Expression Omnibus (GEO) to identify differentially expressed genes (DEGs) between rheumatoid arthritis (RA) patients and healthy controls. Mitophagy-related genes (MRGs) were extracted from the literature and screened using bioinformatics techniques, resulting in differentially expressed MRGs (DE-MRGs). The diagnostic value of these genes was assessed using receiver operating characteristic (ROC) curves, and an ANN model was constructed. In the GSE77298 dataset, 267 differentially expressed genes (DEGs) were identified. Weighted gene co-expression network analysis (WGCNA) identified 2191 key module genes, leading to 63 DE-MRGs. Two MRGs, TMEM45A and ZBTB25, were identified as hub genes with areas under the curve (AUC) of 0.991 and 0.911, respectively. The nomogram model demonstrated high diagnostic value. Mitophagy plays a critical role in the progression of rheumatoid arthritis (RA). Identifying two genes associated with mitophagy may aid in the early diagnosis, mechanistic understanding, and treatment of RA.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"287-303"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the impact of gut microbiota-derived metabolites on depression through network pharmacology. 通过网络药理学揭示肠道微生物衍生代谢物对抑郁症的影响。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-24 DOI: 10.1080/21691401.2025.2531752

Si Su, Tengarile A, Ruhan A, Riga Wu, Lisi Wei, La Ta, Wenfeng Huo, Lijun Tong, Jing Zhang, Rilebagen Hu, Li Li

{"title":"Revealing the impact of gut microbiota-derived metabolites on depression through network pharmacology.","authors":"Si Su, Tengarile A, Ruhan A, Riga Wu, Lisi Wei, La Ta, Wenfeng Huo, Lijun Tong, Jing Zhang, Rilebagen Hu, Li Li","doi":"10.1080/21691401.2025.2531752","DOIUrl":"https://doi.org/10.1080/21691401.2025.2531752","url":null,"abstract":"A total of 208 metabolites and 223 targets were initially extracted from the gutMGene v1.0 database. In addition, 1,630 and 1,321 targets were identified using the Similarity Ensemble Approach and Swiss Target Prediction databases, respectively, resulting in 921 overlapping targets. By integrating data from gutMGenev1.0, 13 core targets were finally identified. A microbiota-metabolite-target-signal pathway-disease network was constructed using Cytoscape 3.9.1, revealing 15 metabolites associated with the IL-17, TLR, and PI3K-Akt signalling pathways. Among these, five metabolites exhibited favourable drug similarity and acceptable toxicological profiles. Molecular docking confirmed the stable binding of two key metabolites-succinate and phenylacetylglutamine-to their respective targets. The results showed that succinate and phenylacetylglutamine demonstrated strong binding affinities to IL-1β and GSK3B, both involved in the IL-17, TLR, and PI3K-Akt signalling pathways. IL-17 and TLR4, as important pro-inflammatory cytokines, are closely associated with the development of depression, while the PI3K/AKT signalling pathway plays a key role in its pathogenesis. The present study reveals the potential mechanisms by which gut microbiota influence MDD and provides a scientific basis and a comprehensive research framework for future investigations.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"327-342"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-14 DOI: 10.1080/21691401.2025.2478352

引用次数: 0

Statement of Retraction: Long noncoding RNA LINC-PINT inhibits non-small cell lung cancer progression through sponging miR-218-5p/PDCD4. 撤回声明：长链非编码RNA LINC-PINT通过海绵miR-218-5p/PDCD4抑制非小细胞肺癌的进展。

IF 4.5 3区生物学

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-21 DOI: 10.1080/21691401.2025.2536932

引用次数: 0