The mechanistic action of mogroside V in the alleviation of oxidative aging.

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-11 DOI:10.1080/21691401.2025.2486752

Qiannan Wang, Xinyue Mao, Yulan Li, Gang Mo, Dayu Li, Deping Cao, Gen Chen

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引用次数: 0

Abstract

Introduce: Diseases related to oxidative ageing are becoming increasingly evident in younger individuals. In this study, we investigated the mechanisms underlying the actions of mogroside V when used to treat anti-oxidative ageing.

Methods: We used D-galactose-induced LO2 cells and C57BL/6J mice as models to investigate the molecular mechanisms of mogroside V (MV) for the treatment of oxidative ageing. Network pharmacology was used to predict the targets of MV for the treatment of oxidative ageing.

Results: By down-regulating the EGFR/p38/JNK pathway, MV significantly inhibited oxidative ageing and apoptosis in cells, reduced the levels of SA-β-galactosidase. In mice, compared with the model group, MV treatment (100 mg/kg·d) reduced MDA levels and significantly increased the levels of GSH and SOD; furthermore, the size and structure of the liver leaflet and glomeruli was arranged in a regular manner; the small intestine glands had decreased in size. Moreover, the expression levels of Ptp1b mRNA had increased significantly while the levels of c-Jun mRNA and protein were significantly reduced. MV also increased the proportion of beneficial bacteria in the small intestine, including Bacteroidales and Lactobacillaceae.

Conclusion: Our analyses revealed that MV can significantly reduce oxidative ageing caused by the accumulation of D-galactose.

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苦参苷V在延缓氧化老化中的作用机制。

简介：与氧化老化有关的疾病在年轻人中变得越来越明显。在这项研究中，我们研究了苦参苷V治疗抗氧化老化的作用机制。方法：以d -半乳糖诱导的LO2细胞和C57BL/6J小鼠为模型，研究槐总苷V （MV）治疗氧化老化的分子机制。利用网络药理学方法预测了MV治疗氧化老化的靶点。结果：MV通过下调EGFR/p38/JNK通路，显著抑制细胞氧化老化和凋亡，降低SA-β-半乳糖苷酶水平。小鼠与模型组比较，MV （100 mg/kg·d）处理降低MDA水平，显著升高GSH和SOD水平；肝小叶和肾小球的大小结构排列规律；小肠腺变小了。Ptp1b mRNA的表达水平显著升高，而c-Jun mRNA和蛋白的表达水平显著降低。MV还增加了小肠中有益菌的比例，包括拟杆菌科和乳酸杆菌科。结论：我们的分析表明，MV可以显著减少d -半乳糖积累引起的氧化老化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

10.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.