Qiannan Wang, Xinyue Mao, Yulan Li, Gang Mo, Dayu Li, Deping Cao, Gen Chen
{"title":"苦参苷V在延缓氧化老化中的作用机制。","authors":"Qiannan Wang, Xinyue Mao, Yulan Li, Gang Mo, Dayu Li, Deping Cao, Gen Chen","doi":"10.1080/21691401.2025.2486752","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduce: </strong>Diseases related to oxidative ageing are becoming increasingly evident in younger individuals. In this study, we investigated the mechanisms underlying the actions of mogroside V when used to treat anti-oxidative ageing.</p><p><strong>Methods: </strong>We used D-galactose-induced LO2 cells and C57BL/6J mice as models to investigate the molecular mechanisms of mogroside V (MV) for the treatment of oxidative ageing. Network pharmacology was used to predict the targets of MV for the treatment of oxidative ageing.</p><p><strong>Results: </strong>By down-regulating the <i>EGFR</i>/<i>p38</i>/<i>JNK</i> pathway, MV significantly inhibited oxidative ageing and apoptosis in cells, reduced the levels of SA-β-galactosidase. In mice, compared with the model group, MV treatment (100 mg/kg·d) reduced MDA levels and significantly increased the levels of GSH and SOD; furthermore, the size and structure of the liver leaflet and glomeruli was arranged in a regular manner; the small intestine glands had decreased in size. Moreover, the expression levels of <i>Ptp1b</i> mRNA had increased significantly while the levels of <i>c-Jun</i> mRNA and protein were significantly reduced. MV also increased the proportion of beneficial bacteria in the small intestine, including <i>Bacteroidales</i> and <i>Lactobacillaceae</i>.</p><p><strong>Conclusion: </strong>Our analyses revealed that MV can significantly reduce oxidative ageing caused by the accumulation of D-galactose.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"166-180"},"PeriodicalIF":4.5000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The mechanistic action of mogroside V in the alleviation of oxidative aging.\",\"authors\":\"Qiannan Wang, Xinyue Mao, Yulan Li, Gang Mo, Dayu Li, Deping Cao, Gen Chen\",\"doi\":\"10.1080/21691401.2025.2486752\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduce: </strong>Diseases related to oxidative ageing are becoming increasingly evident in younger individuals. In this study, we investigated the mechanisms underlying the actions of mogroside V when used to treat anti-oxidative ageing.</p><p><strong>Methods: </strong>We used D-galactose-induced LO2 cells and C57BL/6J mice as models to investigate the molecular mechanisms of mogroside V (MV) for the treatment of oxidative ageing. Network pharmacology was used to predict the targets of MV for the treatment of oxidative ageing.</p><p><strong>Results: </strong>By down-regulating the <i>EGFR</i>/<i>p38</i>/<i>JNK</i> pathway, MV significantly inhibited oxidative ageing and apoptosis in cells, reduced the levels of SA-β-galactosidase. In mice, compared with the model group, MV treatment (100 mg/kg·d) reduced MDA levels and significantly increased the levels of GSH and SOD; furthermore, the size and structure of the liver leaflet and glomeruli was arranged in a regular manner; the small intestine glands had decreased in size. Moreover, the expression levels of <i>Ptp1b</i> mRNA had increased significantly while the levels of <i>c-Jun</i> mRNA and protein were significantly reduced. MV also increased the proportion of beneficial bacteria in the small intestine, including <i>Bacteroidales</i> and <i>Lactobacillaceae</i>.</p><p><strong>Conclusion: </strong>Our analyses revealed that MV can significantly reduce oxidative ageing caused by the accumulation of D-galactose.</p>\",\"PeriodicalId\":8736,\"journal\":{\"name\":\"Artificial Cells, Nanomedicine, and Biotechnology\",\"volume\":\"53 1\",\"pages\":\"166-180\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2025-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Artificial Cells, Nanomedicine, and Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1080/21691401.2025.2486752\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/11 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Artificial Cells, Nanomedicine, and Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/21691401.2025.2486752","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/11 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
The mechanistic action of mogroside V in the alleviation of oxidative aging.
Introduce: Diseases related to oxidative ageing are becoming increasingly evident in younger individuals. In this study, we investigated the mechanisms underlying the actions of mogroside V when used to treat anti-oxidative ageing.
Methods: We used D-galactose-induced LO2 cells and C57BL/6J mice as models to investigate the molecular mechanisms of mogroside V (MV) for the treatment of oxidative ageing. Network pharmacology was used to predict the targets of MV for the treatment of oxidative ageing.
Results: By down-regulating the EGFR/p38/JNK pathway, MV significantly inhibited oxidative ageing and apoptosis in cells, reduced the levels of SA-β-galactosidase. In mice, compared with the model group, MV treatment (100 mg/kg·d) reduced MDA levels and significantly increased the levels of GSH and SOD; furthermore, the size and structure of the liver leaflet and glomeruli was arranged in a regular manner; the small intestine glands had decreased in size. Moreover, the expression levels of Ptp1b mRNA had increased significantly while the levels of c-Jun mRNA and protein were significantly reduced. MV also increased the proportion of beneficial bacteria in the small intestine, including Bacteroidales and Lactobacillaceae.
Conclusion: Our analyses revealed that MV can significantly reduce oxidative ageing caused by the accumulation of D-galactose.
期刊介绍：
Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.
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