Investigating the anticancer and synergistic effects of the combination of green synthesized silver nanoparticles and papaverine on breast cancer cell lines: focusing on the apoptosis pathway and microRNA regulation.

IF 4.5 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Maryam Fekri Soufiabadi, Reza Haji Hosseini, Zolfaghar Lotfi
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引用次数: 0

Abstract

The study investigates the anticancer effects of green silver nanoparticles (Ag-NPs) synthesized from Viola cornuta extract combined with papaverine on breast cancer cells. Ag-NPs were characterized using various analytical techniques, confirming their presence with UV-vis spectroscopy showing a peak at 413 nm and an average size of 42 nm via field emission scanning electron microscopy (FE-SEM) analysis. The particles demonstrated a face-centred cubic structure, with energy-dispersive X-ray spectroscopy (EDX) confirming elemental composition. Additionally, the zeta potential measurement of -6.75 mV indicated favourable electrostatic repulsion between nanoparticles, thereby confirming their stability. Antioxidant activity was significant, with an EC50 value of 38.78 μg/mL. The combination treatment of Ag-NPs and papaverine exhibited synergistic effects, lowering IC50 values to 2.8 + 112.7 μg/mL for MCF-7 cells and 6.2 + 112 μg/mL for MDA-MB-231 cells, without toxicity to normal cells. Flow cytometry revealed G0/G1 phase inhibition and increased sub-G1 populations, indicating cell cycle arrest, alongside increased reactive oxygen species generation and apoptosis. Notably, the experimental group showed altered expression of oncogenic and tumour suppressor microRNAs and apoptotic genes (p < .0001), underscoring the potential of this nanoparticle-papaverine combination as an effective anticancer strategy against breast cancer treatment resistance.

研究绿色合成纳米银与罂粟碱联合对乳腺癌细胞株的抗癌和协同作用:重点研究凋亡途径和microRNA调控。
研究了堇菜提取物与罂粟碱联合合成的绿色银纳米粒子(Ag-NPs)对乳腺癌细胞的抗癌作用。利用各种分析技术对Ag-NPs进行了表征,通过紫外可见光谱(UV-vis)证实了Ag-NPs的存在,通过场发射扫描电镜(FE-SEM)分析发现,Ag-NPs的峰值在413 nm处,平均尺寸为42 nm。粒子表现出面心立方结构,能量色散x射线光谱(EDX)证实了元素组成。此外,zeta电位测量值为-6.75 mV,表明纳米颗粒之间具有良好的静电斥力,从而证实了它们的稳定性。抗氧化活性显著,EC50值为38.78 μg/mL。Ag-NPs与罂粟碱联合作用,MCF-7细胞IC50值降至2.8 + 112.7 μg/mL, MDA-MB-231细胞IC50值降至6.2 + 112 μg/mL,对正常细胞无毒性。流式细胞术显示G0/G1期抑制和亚G1群增加,表明细胞周期阻滞,同时活性氧生成和凋亡增加。值得注意的是,实验组显示了致癌和肿瘤抑制microrna以及凋亡基因的表达改变(p
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来源期刊
Artificial Cells, Nanomedicine, and Biotechnology
Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL
CiteScore
10.90
自引率
0.00%
发文量
48
审稿时长
20 weeks
期刊介绍: Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.
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