Aquaculture Nutrition最新文献

{"title":"Isolation of a Protease-Producing Microbacterium From the Intestinal Tract of Ruditapes philippinarum and Its Probiotic Effects","authors":"Hui Guan,&nbsp;Yaodong Qiu,&nbsp;Mingyue Guan,&nbsp;Sitong Quan,&nbsp;Shang Liu,&nbsp;Yingxu Chen,&nbsp;Haochen Li,&nbsp;Haolin Yu,&nbsp;Lei Fang,&nbsp;Zhongming Huo","doi":"10.1155/anu/8865049","DOIUrl":"https://doi.org/10.1155/anu/8865049","url":null,"abstract":"<p>Aquatic probiotics are generally recognized as safe and nontoxic live bacterial preparations, primarily sourced from the culturing environment or directly from aquatic organisms. Upon entering the aquatic environment, these probiotics are capable of colonizing both the surface and internal compartments of aquatic organisms, thereby enhancing their nutrient absorption and immune defense. The intestinal bacteria of aquatic animals represent a significant source of probiotics, as they possess inherent advantages in colonizing the intestinal tracts of their hosts and competing with the established intestinal microbiota. This study was designed to isolate probiotics from <i>Ruditapes philippinarum</i> by screening culturable bacteria harvested from the intestines of healthy clams. Using selective culture media and a protease-producing test kit, a protease-producing bacterium that did not form a β-hemolytic ring on Columbia blood agar plates was successfully isolated and designated as Strain 48. Subsequent morphological analysis, 16S rDNA and whole-genome sequencing–based molecular identification, and physiological and biochemical profiling confirmed that Strain 48 belonged to the species of <i>Microbacterium forte</i>. Based on the whole-genome sequencing results, further analyses were conducted to explore its functional potential and saftety-related genetic characteristics. Immersion bacterial challenge tests revealed that Strain 48 exhibited no toxicity to <i>R. philippinarum</i> at concentrations of 1 × 10⁶, 1 × 10⁷, and 1 × 10⁸ CFU/mL. Culture experiments conducted over a 60-day period demonstrated that the group exposed to Strain 48 at a concentration of 1 × 10⁷ CFU/mL exhibited significantly increased weight gain and shell length growth compared to the control group, and lysozyme (LZM) activity was also significantly enhanced (<i>p</i> &lt; 0.01). Genomic analysis indicated that virulence factors or antibiotic resistance genes (ARGs) associated with mobile genetic elements were absent, which supported the biosafety of Strain 48. Administration of Strain 48 significantly increased LZM activity in the soft tissues of <i>R. philippinarum</i> (<i>p</i> &lt; 0.01), indicating an enhanced host immune response. Overall, Strain 48 may have the potential to be developed as a probiotic for <i>R. philippinarum</i>.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/8865049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147566916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Practical Diets Formulated With Different Plant-Based Energy Ingredients Modulate Nutrient Utilization and Gut Morphology in Tambaqui (Colossoma macropomum)","authors":"Jeisson Emerson Casemiro Ferrari,&nbsp;Maria Karolaine Moriman Delgado,&nbsp;Gabriela Castellani Carli,&nbsp;Douglas de Souza Graciano,&nbsp;Jony Koji Dairiki,&nbsp;Leonardo Susumu Takahashi","doi":"10.1155/anu/6643007","DOIUrl":"https://doi.org/10.1155/anu/6643007","url":null,"abstract":"<p>Despite the increasing use of plant-based energy ingredients in aquaculture, few studies have evaluated the digestibility of practical diets formulated with these ingredients in an integrated manner with the metabolic responses, intestinal health, and productive performance of fish. This limits the understanding of their nutritional effects. This study evaluated the apparent digestibility of nutrients and energy from starch-rich practical diets and their effects on growth performance, intestinal fold histomorphometry, and metabolic and physiological profiles of tambaqui (<i>Colossoma macropromum</i>). One hundred and ninety-two tambaqui juveniles (131.63 ± 1.94 g) were fed six experimental, pelleted diets formulated with corn (CO), cornstarch (CS), sorghum (SO), wheat bran (WB), rice bran (RB), or broken rice (BR). The apparent digestibility coefficients of dry matter (ADC_DM), organic matter (ADC_OM), ether extract (ADC_EE), crude protein (ADC_CP), gross energy (ADC_GE), and starch (ADC_ST) were analyzed. ADC_DM (31.8%–71.9%), ADC_OM (44.5%–76.6%), and ADC_GE (48.0%–72.6%) varied among the diets but followed a similar pattern: values for the diets with CO (63.3%, 71.7%, and 72.6%, respectively), CS (71.9%, 76.6%, and 79.2%, respectively), and broken bran (65.6%, 72.4%, and 72.5%, respectively) were higher than those for the diets with WB (41.0%, 52.2%, and 53.8%, respectively) and RB (31.8%, 44.5%, and 48.0%, respectively). ADC_CP and ADC_EE were &gt; 70% in all diets, except that with WB. ADC_ST was &gt; 90% for all diets. Plasma glucose and serum cholesterol concentrations were similar across treatments. Diets including WB and RB resulted in less-developed intestinal folds, which led to lower digestibility and, consequently, reduced growth. In contrast, diets formulated with SO, BR, or CS proved to be viable alternatives to the CO-based diet, promoting comparable nutrient and energy digestibility. Tambaqui exhibited metabolic adaptation to the physicochemical properties of these formulations, maintaining productive performance. However, the inclusion of WB and RB should be limited because of the high fiber content, which can compromise intestinal health and nutrient utilization efficiency.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/6643007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147566915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Feeding Tiger Puffer (Takifugu rubripes) With Trash Fish and Formulated Feeds on Growth Performance, Flesh Quality, Antioxidant, and Immunity Capacity","authors":"Zhen Xu,&nbsp;Hang Yang,&nbsp;Xiaoying Xu,&nbsp;Weidong Li,&nbsp;Huawei Li,&nbsp;Wenbing Zhang,&nbsp;Chenghui Li,&nbsp;Haien Zhang,&nbsp;Zhenda Zhang","doi":"10.1155/anu/6685020","DOIUrl":"https://doi.org/10.1155/anu/6685020","url":null,"abstract":"<p>This study evaluated the effects of replacing trash fish (TF) with formulated feeds in tiger puffer (<i>Takifugu rubripes</i>) on the growth performance, liver and serum biochemical indices, flesh quality, and metabolomics. Fish (39.6 ± 0.2 g) were fed TF, commercial feed (CF), and self-formulated feed (SF) for 8 weeks. Results indicated that CF and SF groups had significantly lower feed conversion ratio (FCR) and feed intake (FI) and higher protein efficiency ratio (PER) compared to TF group (<i>p</i>&lt;0.05). CF and SF groups exhibited lower flesh protein and collagen contents and shear force than TF group, with CF group showing lower flesh hardness, chewiness, and catalase (CAT) activity, and SF group showing higher superoxide dismutase (SOD) activity and lower malondialdehyde (MDA) content (<i>p</i> &lt; 0.05). Compared to TF group, serum aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities were reduced and liver triglyceride (TG) and cholesterol (CHO) contents were increased in SF group (<i>p</i> &lt; 0.05). SF and CF groups exhibited significantly lower levels of lysine (Lys), glycine (Gly), proline (Pro), cysteine (Cys), and total amino acids (TAAs) and higher flavor amino acids (FAAs) level in flesh (<i>p</i>&lt;0.05). Moreover, SF group significantly increased contents of C18:2<i>n</i>−6 and <i>n</i>−6 polyunsaturated fatty acids (<i>n</i>−6 PUFAs) and decreased <i>n</i>−3/<i>n</i>−6 ratio (<i>p</i>&lt;0.05). Metabolomic analysis identified altered amino acid and lipid metabolism pathways. In summary, formulated feeds can effectively replace TF in tiger puffer diets; however, further optimization of amino acid and fatty acid profiles is required to improve flesh quality and nutritional value.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/6685020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147566240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics Reveals the Molecular Mechanism of Collagen Synthesis by Hydrolyzed Collagen Supplementation of Large Yellow Croaker (Larimichthys crocea)","authors":"Fangle Tong,&nbsp;Xuexue Zhang,&nbsp;Lina Zhang,&nbsp;Xiaocheng Deng,&nbsp;Yalan Zhang,&nbsp;Shi Wang,&nbsp;Zehong Wei","doi":"10.1155/anu/3209384","DOIUrl":"https://doi.org/10.1155/anu/3209384","url":null,"abstract":"<p>This study investigated the effects of dietary hydrolyzed collagen supplementation on muscle quality and its underlying molecular mechanisms in large yellow croaker (<i>Larimichthys crocea</i>) (initial weight: 332.27 ± 3.79 g) over a 128-day feeding trial. The results demonstrated that dietary supplementation with 6% hydrolyzed collagen significantly increased muscle collagen content and collagen volume fraction (CVF) in both female and male fish (<i>p</i> &lt; 0.05). Furthermore, hydrolyzed collagen significantly enhanced muscle springiness in females, as well as springiness and chewiness in males (<i>p</i> &lt; 0.05). Multiomics analysis revealed substantial alterations in gene expression and DNA methylation: 1146 differentially expressed genes (DEGs) and 4655 differentially methylated regions (DMRs) were identified in females, while 2460 DEGs and 5846 DMRs were detected in males. Functional enrichment analysis indicated that differentially methylated genes (DMGs) in females were predominantly associated with pathways related to muscle cell morphology and differentiation, such as “regulation of actin cytoskeleton.” In contrast, DMGs in males were significantly enriched in pathways governing cell proliferation, adhesion, and migration, such as “MAPK signaling pathway” and “focal adhesion.” Integrative analysis of RNA sequencing (RNA-seq) and whole-genome bisulfite sequencing (WGBS) confirmed a negative correlation between DNA methylation and gene expression, identifying key collagen synthesis-related genes regulated by methylation, including <i>col6a2</i>, <i>col4a5</i>, and <i>hat1</i> in females, and <i>col6a2</i>, <i>col27a1b</i>, <i>col12a1</i>, <i>p4ha1</i>, <i>loxl2</i>, <i>tgfβr2</i>, and <i>bmp1</i> in males. This study reveals that hydrolyzed collagen enhances collagen deposition and textural properties by modulating collagen synthesis networks via DNA methylation, providing a molecular basis for its application in aquafeed.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/3209384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147566447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Glutamine Positively Affects Growth Performance and Hepatic-Intestinal Health of Paramisgurnus dabryanus","authors":"Youjie Li,&nbsp;Yaping Li,&nbsp;Qiubai Zhou,&nbsp;Jie Pan,&nbsp;Qi Chen,&nbsp;Wenshuo Liu,&nbsp;Yuhong Liao,&nbsp;Shujun Liao,&nbsp;Chuanqi Yu,&nbsp;Zirui Wang","doi":"10.1155/anu/9719734","DOIUrl":"https://doi.org/10.1155/anu/9719734","url":null,"abstract":"<p>This study aims to evaluate the effects of dietary glutamine on the growth performance and hepatic-intestinal health of loach, <i>Paramisgurnus dabryanus</i>. Loaches were fed diets with graded glutamine concentrations of 0% (control), 0.25%, 0.50%, 0.75%, 1.00%, and 1.25% for 8 weeks. The results indicated that inclusion of 0.75% and 1.00% glutamine in the diet significantly improved the weight gain rate (WGR) and specific growth rate (SGR), and the optimal supplementation level of glutamine in the diet was 0.74% based on quadratic regression analysis of WGR. Further biochemical and histological analyses revealed that the 0.75% glutamine group demonstrated significant elevations in hepatic aspartate transaminase (AST), glutathione peroxidase (GPX) and catalase (CAT) activities, glutathione (GSH) and immunoglobulin M (IgM) contents, and intestinal amylase activity relative to the control (<i>p</i> &lt; 0.05). Moreover, the intestinal villus height (VH), villus width (VW), and muscular layer thickness (MT) were increased with the increasing concentration of glutamine in the diet, reaching their peak values in the 1.00% glutamine group. In summary, 0.74% dietary glutamine is recommended to optimize growth performance and hepatic-intestinal health in loach.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/9719734","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147565838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermented Rapeseed Meal Mitigates Intestinal and Hepatic Injuries via Gut Microbiota Modulation and Autophagy Activation in Koi Carp (Cyprinus carpio)","authors":"Rong Zhang,&nbsp;Wanning Mao,&nbsp;Lili Liu,&nbsp;Xiaowen Wang,&nbsp;Huijuan Li,&nbsp;Hua Zhu","doi":"10.1155/anu/2092050","DOIUrl":"https://doi.org/10.1155/anu/2092050","url":null,"abstract":"<p>This study assessed whether fermented rapeseed meal (FRSM) could alleviate the negative impacts of rapeseed meal (RSM) on growth, intestinal health, and liver function in koi carp (<i>Cyprinus carpio</i>). Eighty-one koi carp were randomly divided into three dietary groups: a control group receiving a soybean meal (SBM) diet, an unfermented RSM group, and a FRSM group. Growth performance, serum biochemical parameters, tissue histology, gut microbiota, and transcriptomic profiles were assessed. The findings showed that the RSM and FRSM groups had notably lower weight gain, weight gain rate, and specific growth rate (SGR) compared to the SBM group (<i>p</i> &lt; 0.05). In the RSM and FRSM groups, serum antioxidant enzyme activities (SOD, CAT, and GSH-PX) significantly decreased, whereas MDA levels and AST activity significantly increased (<i>p</i> &lt; 0.001). Histological analysis revealed that intestinal villus structures remained intact in all groups, but the FRSM group exhibited a significant reduction in crypt depth (<i>p</i> = 0.019). Scanning electron microscopy (SEM) showed that the SBM group had a greater microvilli density than the RSM and FRSM groups. Severe hepatic pathological changes were observed in the RSM group, whereas hepatic damage was notably alleviated in the FRSM group. LEfSe analysis identified the <i>Ruminococcus torques</i> group as a distinctive biomarker in the FRSM group, showing significant enrichment relative to both the SBM and RSM groups. RSM induced significant transcriptomic alterations in the intestine and liver, characterized by increased oxidative stress, metabolic dysfunction, and activation of inflammatory pathways. FRSM transformed these responses by enhancing autophagy, lipid metabolism, and cellular repair mechanisms, indicating a protective and regenerative effect. Quantitative PCR analysis validated the expression trends detected in the omics. In conclusion, dietary supplementation with FRSM may alleviate intestinal and hepatic injuries induced by high-level RSM inclusion, potentially through gut–liver axis modulation, enrichment of <i>Ruminococcus torques group</i>, and activation of autophagy-related pathways.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/2092050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147565076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Replacing Fishmeal With Antarctic Krill Meal on Nutrient Deposition, Metabolism, and Immunity in Silver Pomfret (Pampus argenteus)","authors":"Guangde Qiao,&nbsp;Yabing Wang,&nbsp;Qiaozhen Ke,&nbsp;Shengyu Liu,&nbsp;Xiaoshan Wang,&nbsp;Shuaijie Wang,&nbsp;Shiming Peng","doi":"10.1155/anu/4095616","DOIUrl":"10.1155/anu/4095616","url":null,"abstract":"<p>Although silver pomfret (<i>Pampus argenteus</i>) is a highly valued marine fish in China, its aquaculture development is limited by the lack of species-specific formulated feed. This study investigated the effects of replacing fishmeal with Antarctic krill (<i>Euphausia superba</i>) meal (AKM) on the nutritional deposition, metabolism, and immune response of silver pomfret. Juvenile fish with an initial body weight of 12.93 ± 0.48 g were randomly allocated into four dietary treatments with three replicates per treatment (50 fish per tank; 600 fish in total), and fed one of four experimental diets containing 0% (FM), 10% (KM10), 20% (KM20), or 40% (KM40) AKM for a 60-day feeding trial. The results showed that moderate AKM inclusion, particularly at the 20% replacement genes (<i>pparα</i>, <i>cpt1α</i>) and the downregulation of lipid level significantly enhanced intestinal trypsin and lipase activities, as well as glucose and amino acid metabolic capacity. In addition, lipid utilization efficiency was improved through the upregulation of fatty acid oxidation-related genes (<i>fas</i>). Consequently, the KM20 group exhibited significantly higher muscle essential amino acids and polyunsaturated fatty acids, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), compared to the FM group (<i>p</i> &lt; 0.05). Furthermore, serum immunoglobulin M (IgM), complement C3, and C4 levels increased in AKM-fed groups, with KM20 showing the most pronounced enhancement. However, excessive substitution (KM40) led to a decline in certain nutritional and immune parameters, suggesting potential metabolic imbalances. These findings indicate that moderate replacement of fishmeal with AKM (~20%) optimizes nutrient deposition, enhances metabolic efficiency, and boosts immune capacity in silver pomfret. This study provides a theoretical basis for the development of functional aquafeeds aimed at promoting the sustainable and efficient industrial cultivation of silver pomfret.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12976148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth Performance and Intestinal Health of Triploid Rainbow Trout (Oncorhynchus mykiss) Through Bile Acid Supplementation in Low Fishmeal Diets: Insights From Metabolomics, Microbiota, and Gene Expression","authors":"Huamin Wang,&nbsp;Siyuan Liu,&nbsp;Zhenhua Ma,&nbsp;Shidi Wang,&nbsp;Shuze Zhang,&nbsp;Shaoxia Lu,&nbsp;Shicheng Han,&nbsp;Haibo Jiang,&nbsp;Gefeng Xu,&nbsp;Hongbai Liu,&nbsp;Chang’an Wang","doi":"10.1155/anu/8260382","DOIUrl":"10.1155/anu/8260382","url":null,"abstract":"<p>This research aimed to explore the impact of bile acid (BA) supplementation in low fishmeal diets on growth performance, serum biochemical indices, tissue morphology, lipid metabolism, gene expression, and gut microbiota in triploid rainbow trout (<i>Oncorhynchus mykiss</i>, initial weight 15.97 ± 1.4 g). The experimental design involved a low fishmeal diet (10%), and four treatment groups with BA additions (0.05%, 0.10%, 0.15%, and 0.20%) to the basal diet. The findings indicated that the group receiving 0.10% BA (G3) exhibited an enhancement in final body weight (FBW), specific growth rate (SGR), weight gain rate (WGR), and condition factor (CF), significantly outperforming the control group (G1, <i>p</i> &lt; 0.05). 0.10% BA addition significantly increased whole-body crude protein and lipid content. (<i>p</i> &lt; 0.05). Serum analysis showed a significant reduction in total bilirubin (TBIL) and triglycerides (TG), and an increase in BA and lipoprotein cholesterol (LDL-C) in the G3 group compared to G1 (<i>p</i> &lt; 0.05). The 0.10% BA supplementation downregulated pro-inflammatory gene expression, like <i>il-1β</i>, and upregulated lipid metabolism-related genes, like <i>scdb</i>, in the intestinal tract of <i>O. mykiss</i> (<i>p</i> &lt; 0.05). 16S high-throughput sequencing identified key microbial groups in the intestine of <i>O. mykiss</i>, highlighting significant differences in microbial composition with BA supplementation. Metabolomic analysis revealed that BA addition altered the metabolic profile of <i>O. mykiss</i>, affecting pathways such as polycyclic aromatic hydrocarbon (PAH) catabolism, cysteine (Cys) and methionine (Met) metabolism, the sulfur relay system, arachidonic acid (AA) metabolism, and ovarian steroidogenesis. In summary, a 0.10% BA addition to the diet of <i>O. mykiss</i> significantly improved growth performance, mitigated intestinal inflammation in specimens fed low fishmeal diets, and promoted overall gut health and lipid metabolism.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147375808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Dietary Taurine on the Innate Immune Responses, Digestive Function, and mTOR Signaling in Coho Salmon (Oncorhynchus kisutch)","authors":"Linxuan Bian,&nbsp;Ya Wang,&nbsp;Xinran Zhang,&nbsp;Miao Zhang,&nbsp;Dongwu Liu","doi":"10.1155/anu/7769837","DOIUrl":"10.1155/anu/7769837","url":null,"abstract":"<p>In this study, the effect of dietary taurine (Tau) on the innate immune responses, digestive function, and mammalian target of rapamycin (RAPA; mTOR) signaling was investigated in coho salmon (<i>Oncorhynchus kisutch</i>). Coho salmon were fed diets supplemented with 0, 0.2, 0.4, and 0.6 g/kg Tau for 8 weeks. The results demonstrated that the activity of antioxidant enzymes was enhanced, while reactive oxygen species (ROS) production was reduced by dietary Tau in the small intestine. Furthermore, Tau supplementation altered the homeostasis of essential nutrients, including Na, K, and Ca. Dietary Tau enhanced intestinal barrier function by upregulating tight junction proteins, including occludin, ZO-1, and claudin-18 gene expression. Dietary Tau also upregulated the gene expression of inflammatory cytokines such as IL-1β, TNFα, and IL-6, as well as the gene expression related to mTOR signaling pathway. Notably, Tau positively influenced intestinal villus morphology and trypsin activity and increased levels of free amino acids. In addition, the mTOR inhibitor RAPA was used to reveal mTOR’s role in regulating the expression of molecules associated with innate immune responses. The results showed that RAPA treatment suppressed the gene expression related to antioxidant enzymes and intestinal tight junction. In conclusion, Tau could modulate innate immune responses, digestive function, and intestinal barrier integrity, and mTOR may play a role in regulating these physiological processes in the small intestine of coho salmon.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12956379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blended Oil With an Optimized Fatty Acid Profile Improves Fish Meal Substitution Efficacy in Carnivorous Teleost Largemouth Bass Diet","authors":"Junfeng Guan,&nbsp;Jianzhao Xu,&nbsp;Xin Gao,&nbsp;Zekui Huang,&nbsp;Chao Xu,&nbsp;Ermeng Yu,&nbsp;Dizhi Xie,&nbsp;Yuanyou Li","doi":"10.1155/anu/8841385","DOIUrl":"10.1155/anu/8841385","url":null,"abstract":"<p>Several factors are known to affect the substitution of fish meal (FM) in aquafeeds, yet the influence of dietary fatty acid (FA) composition remains unclear. To investigate this, fish oil (FO), an FA-optimized blended oil (BO1) designed to meet the essential FA (EFA) requirements of largemouth bass (<i>Micropterus salmoides</i>), and a blend rich in n-6 polyunsaturated FAs (PUFAs) (BO2, a 2:3 mixture of FO and soybean oil) were used as dietary lipid sources. Three isoproteic (50%) and isolipidic (9%) diets with distinct FA profiles were formulated at either 24% (24FO, 24BO1, and 24BO2) or 16% (16FO, 16BO1, and 16BO2) FM inclusion levels. Juvenile fish (initial weight about 12.50 g) were fed the diets for 10 weeks. Results showed no significant differences in growth performance among the 24% FM groups. At the 16% FM level, the 16BO1 group exhibited growth comparable to the 16FO group and achieved significantly higher final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR) than the 16BO2 group (<i>p</i> &lt; 0.05). Moreover, compared to 16BO2, the 16BO1 group demonstrated improved lipid metabolism (indicated by reduced hepatosomatic index [HSI], viscerosomatic index [VSI], triglycerides [TGs], nonesterified FAs [NEFAs], and blood urea nitrogen [BUN]), enhanced protein synthesis (reflected in increased total amino acids [TAAs], alanine transaminase [ALT], and aspartate transaminase [AST]), elevated antioxidant capacity (total antioxidant capacity [T-AOC] and catalase [CAT]), and upregulated mRNA expression of genes related to lipid oxidation (<i>pparα</i>, <i>atgl</i>, and <i>acsl4</i>) and protein synthesis (<i>akt2</i> and <i>eif4g</i>). These findings demonstrate that optimizing dietary FA composition enhances FM substitution efficacy by promoting lipid-based energy supply, improving protein synthesis, and strengthening antioxidant responses. This study is the first to reveal that dietary FA profiles modulate FM replacement efficiency in aquatic feeds, providing new insights and viable strategy for developing low-FM diets to promote sustainable largemouth bass aquaculture.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12948731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147324537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}