Aquaculture Nutrition最新文献

{"title":"Cholesterol Accumulation Caused Oxidative Stress Associated With Impaired Antioxidant Capability and Mitochondrial Function in the Liver of Nile Tilapia","authors":"Zhixiao Liu,&nbsp;Yi Xiao,&nbsp;Jiaying Xie,&nbsp;Huiwen Zhang,&nbsp;Qiming Huang,&nbsp;Óscar Monroig,&nbsp;Douglas R. Tocher,&nbsp;Xiaojuan Liu,&nbsp;Fan Lin,&nbsp;Cuiying Chen,&nbsp;Shuqi Wang,&nbsp;Ruixin Li","doi":"10.1155/anu/7643962","DOIUrl":"https://doi.org/10.1155/anu/7643962","url":null,"abstract":"<p>In mammals, cholesterol accumulation in tissues often results in health damage, such as oxidative stress. In contrast, the adverse effects of cholesterol accumulation on the physiological health of fish remain largely unexplored. The present study investigated the impacts of cholesterol accumulation on oxidative stress and the potential mechanisms involved in Nile tilapia (<i>Oreochromis niloticus</i>). Thus, Nile tilapia were fed either a control diet (C) or a high-cholesterol (1.6%, HC) diet for 8 weeks. The viscero-somatic (VSIs) and hepatosomatic indices (HSIs) were increased significantly in fish fed the HC diet and, in accordance, HC intake caused the accumulation of cholesterol in the liver, intestine, head kidney, and spleen. Intake of the HC diet, affected oxidative stress as evidenced by elevated malondialdehyde (MDA) levels in the liver and head kidney and reduced catalase (CAT) activities in the liver and spleen, while SOD activities were increased in the spleen and intestine. Moreover, cholesterol accumulation induced endoplasmic reticulum (ER) stress, inflammation, and apoptosis in the liver and head kidney, as evidenced by increased expression levels of key genes. Metabolome analysis indicated that metabolite levels in tilapia fed the HC diet were primarily enriched in glutathione metabolism and the tricarboxylic acid cycle (TCA), with significantly reduced levels of glutamine, glutamate, glycine, citrate, isocitrate, aconitate, malate, and oxalate. In addition, transmission electron microscopy (TEM) analysis showed accumulation of lipid droplets and distinct alterations in the morphology of mitochondria within hepatocytes of tilapia fed HC. Moreover, significantly increased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were found in fish fed the HC diet. Overall, the data suggested that HC intake induced oxidative stress, which might be associated with impaired antioxidant capability and mitochondrial function, as mitochondria are the primary site of producing cellular reactive oxygen species (ROS). The present study is the first to report the impacts of cholesterol accumulation on oxidative stress and health damage in fish, and suggested targeted cholesterol-lowering interventions as a promising therapeutic strategy for addressing health issues in aquatic animals.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/7643962","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Interactive Effects of Dietary Fish Oil and Selenium Nanoparticles Increased Growth, Antioxidant Capacity, and Immune-Related Genes Transcription Level in Penaeus vannamei Reared in Hypersaline Water","authors":"Ali Ahmadi,&nbsp;Vahid Yavari,&nbsp;Mansour Torfi Mozanzadeh,&nbsp;Seyed Mohammad Mousavi,&nbsp;Preeta Kochanian,&nbsp;Ahmad Ghasemi","doi":"10.1155/anu/4165191","DOIUrl":"https://doi.org/10.1155/anu/4165191","url":null,"abstract":"<p>A 60-day research was conducted to evaluate the influence of dietary fish oil (FO) and selenium nanoparticles (SeNPs) on performance of <i>Penaeus vannamei</i> juveniles (2.4 ± 0.0 g) reared in seawater (SW) or hypersaline (HS) water conditions. A 2 × 2 × 2 factorial research was designed with two FO levels, including 3% and 6%, two SeNPs dosages, including 0.4 and 0.8 mg/kg and two water salinity, including SW (35 g/L) and HS (50 g/L). Eight experimental groups were designed as follow: (1) FO₃Se^0.4SW (3% FO + 0.4 mg/kg SeNPs reared in SW), (2) FO₃Se^0.8SW (3% FO + 0.8 mg/kg SeNPs reared in SW), (3) FO₆Se^0.4SW (6% FO + 0.4 mg/kg SeNPs reared in SW), (4) FO₆Se^0.8SW (6% FO + 0.8 mg/kg SeNPs reared in SW), (5) FO₃Se^0.4HS (3% FO + 0.4 mg/kg SeNPs reared in HS), (6) FO₃Se^0.8HS (3% FO + 0.8 mg/kg SeNPs reared in HS), (7) FO₆Se^0.4HS (6% FO + 0.4 mg/kg SeNPs reared in HS), and (8) FO₆Se^0.8HS (6% FO + 0.8 mg/kg SeNPs reared in HS). Four hundred and eighty <i>P. vannamei</i> were randomly distributed into 24 250-L cylindrical fiberglass tanks. Each experimental group was replicated in three tanks (20 shrimp/tank). Shrimps were fed at 5% of their initial biomass three times daily. Water temperature and dissolved oxygen levels were maintained at 31.5 ± 1.2°C and 5.5 ± 1.0 mg/L, respectively. Shrimp in FO₆Se^0.4SW and FO₆Se^0.8SW had higher weight gain (WG) compared to those in FO₃Se^0.8SW and FO₃Se^0.4HS (<i>p</i> &lt; 0.05). Rearing shrimp in HS water increased whole-body protein and ash contents but reduced moisture level. The amount of docosahexaenoic acid (DHA) in FO₃Se^0.4SW, FO₆Se^0.4SW, FO₆Se^0.8SW, and FO₆Se^0.4HS was relatively higher than the other treatments. The antioxidant activities, including glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) level decreased and malondialdehyde (MDA) content in hepatopancreas increased in shrimp reared in HS water. Finally, the transcription levels of insulin-like growth hormones and immune-related genes, including lectin (<i>lec</i>), peneidine 3 (<i>pen-3</i>), prophenoloxidase (<i>propo</i>), and lysozyme (<i>lyz</i>) increased by increasing dietary FO level of 6%. Additionally, these levels were modulated by the interaction of water salinity and dietary SeNPs and FO levels. Based on the findings of the current study, increasing the dietary FO level to 6% and incorporating a moderate level of SeNPs (0.4 mg/kg) were found to enhance <i>P. vannamei</i> tolerance to HS water.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/4165191","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential Application of Prebiotic Stevia rebaudiana in Aquaculture and Animal Feed","authors":"Akram Ismael Shehata,&nbsp;Shengkang Li,&nbsp;Mayada Alhoshy,&nbsp;Hafez A. H. Mabrouk,&nbsp;Ghada R. Sallam,&nbsp;Mohamed A. Al-Absawey,&nbsp;Islam I. Teiba,&nbsp;Kumbukani Mzengereza,&nbsp;Walied M. Fayed,&nbsp;Yusuf Jibril Habib,&nbsp;Mohammed F. El Basuini","doi":"10.1155/anu/3858421","DOIUrl":"https://doi.org/10.1155/anu/3858421","url":null,"abstract":"<p>Aquaculture and animal producers are increasingly exploring natural additives such as <i>Stevia rebaudiana</i> for their health-promoting and sustainability-enhancing roles. Known primarily as a sweetener, <i>S. rebaudiana</i> also contains bioactive compounds, such as stevioside and rebaudioside A (Reb A), which exhibit antibacterial, antioxidant, immunomodulatory, and metabolic benefits. Recent studies suggest that these compounds may also exert prebiotic-like activities by modulating the gut microbiota, promoting the growth of beneficial bacterial populations (e.g., <i>Lactobacillus</i>, <i>Bifidobacterium</i> spp.), and improving intestinal integrity, thereby contributing to enhanced nutrient absorption and immune competence. This review highlights the potential of <i>S. rebaudiana</i> in animal feed and aquaculture, demonstrating its ability to enhance growth performance, strengthen immune systems, regulate fat metabolism, and improve overall animal health. Its role as a functional feed ingredient is further emphasized by its capacity to reduce oxidative stress and enhance product quality in both aquatic and terrestrial species (e.g., carp, mullet, tilapia, shrimp, laying hens, broiler, and goats), all of which align with established markers of prebiotic efficacy. By incorporating <i>S. rebaudiana</i> into animal and aquaculture diets, producers may not only improve animal productivity and welfare but also address sustainability challenges by reducing reliance on synthetic additives and antibiotics. Thus, <i>S. rebaudiana</i> represents a promising, multifunctional ingredient with potential prebiotic activity, supporting both animal health and environmentally responsible agricultural practices.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/3858421","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Dietary Feeding Levels of Juvenile Red-Tail Catfish (Hemibagrus wyckioides) in Land-Based Circular Tank: Insights From Metabolomics and Microbial Analysis","authors":"Baohong Xu,&nbsp;Haibin Hou,&nbsp;Tiaoyi Xiao,&nbsp;Changjun Chen,&nbsp;Qiaolin Liu","doi":"10.1155/anu/5521491","DOIUrl":"https://doi.org/10.1155/anu/5521491","url":null,"abstract":"<p>To investigate the most effective feeding level for red-tail catfish (<i>Hemibagrus wyckioides</i>) raised in land-based circular tanks and the impact of feeding levels on <i>H. wyckioides</i> at the molecular and omics levels, we conducted a 56-day experiment using the fish fries (16.49 ± 0.44 g). Three groups were established with varying feeding levels: 2% (T2), 3% (T3), and 4% (T4) of body weight per day. We compared conventional growth and physiological parameters, transcription levels of antioxidant activity, transforming growth factor (tgf), inflammatory factors, and lipid metabolism genes as well as intestinal microbiota and metabolomics. Our results showed that the feed conversion rate (FCR) in the T2 group was significantly lower than those in the other groups, but there were no significant differences in specific growth rate (SGR) and weight gain rate (WGR) among the groups. The T2 group had significantly higher level of albumin compared to the T4 group, while total protein (TP) and low-density lipoprotein cholesterol (LDL-C) levels were significantly lower than the T3 group. The T2 group also have significantly higher activities of superoxide dismutase (SOD) and alkaline phosphatase (ALP) compared to the other groups, and both alanine transaminase activity and goblet cells (GCs) were markedly elevated in the T2 group compared to the T3 group. Additionally, the T2 group had the lowest Firmicutes/Bacteroidetes ratio, with an increase in <i>Turicibacter</i> and <i>Clostridium</i>. The differential metabolites in the T2 group were significantly upregulated in amino acid metabolism and lipid metabolism-related pathways. The expression levels of antioxidant genes, tgf, anti-inflammatory factors, and lipid metabolism genes were all markedly elevated in the T2 group. These findings suggest that the optimal feeding level for <i>H. wyckioides</i> was 2% of body weight per day. These results can serve as a guide for the scientific aquaculture of <i>H. wyckioides</i>.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/5521491","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Flavor Compounds in Crab Roe and Paste From Chinese Mitten Crab Raised in Lake, Pond, and Rice-Field Environments","authors":"Feifei Han,&nbsp;Lulu Zhou,&nbsp;Lu Jin,&nbsp;Yuqing Zhao,&nbsp;Qihan Zhang,&nbsp;Weilin Liu,&nbsp;Jianzhong Han","doi":"10.1155/anu/4899168","DOIUrl":"https://doi.org/10.1155/anu/4899168","url":null,"abstract":"<p>The Chinese mitten crab (<i>Eriocheir sinensis</i>) is an economically important crab species in China, with a unique flavor, excellent nutritional quality and popularity with consumers. However, the current research on Chinese mud crabs predominantly centers on fresh samples and gives limited attention to the flavor characteristics of crabs after steaming. Consequently, this study aims to explore the flavor differences in crab roe and crab paste following steaming across three distinct aquaculture environments: lakes, ponds, and paddy fields. The overall flavor profile was analyzed by electronic nose and electronic tongue. Nonvolatile and volatile substances were determined by HPLC and SPME-GC–MS-O. Subsequently, the taste intensity values (TAVs) and relative odor activity values (ROAV) were calculated. The results demonstrated that the electronic tongue and electronic nose were capable of distinctly discriminating the differences in crab roe and crab juice among the three types of farmed crabs. The levels of umami amino acids and the equivalent umami concentration (EUC) in the roe and paste of pond crabs (PC) were significantly higher compared to those of lake crabs (LC) and rice-field crabs (RC). Significant variations were observed in both the content and types of flavor compounds in the roe and paste of crabs originating from different aquaculture environments. This study provided a basis for improved understanding of the mechanisms of flavor formation in the Chinese mitten crab and provided a basis for regulating its flavor quality.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/4899168","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144897390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment of Artemia With Synbiotic and Its Effects on Growth Nutrient Utilization Survival and Gut Microbial Communities of Larval Hybrid Catfish (Clarias microstomus × Clarias gariepinus)","authors":"Arlene Debbie Lingoh,&nbsp;Kamil Latif,&nbsp;Yih Nin Lee,&nbsp;Lirong Yu Abit,&nbsp;Shahrul Razid Sarbini,&nbsp;Miguel Isaiah Vincent Mojilis,&nbsp;Fatin Maisarah Shamsul Azhar,&nbsp;Sabrina Rancang Khairul,&nbsp;Sui Sien Leong","doi":"10.1155/anu/6616288","DOIUrl":"https://doi.org/10.1155/anu/6616288","url":null,"abstract":"<p><i>Artemia</i> (brine shrimp) is a vital live feed in aquaculture, providing essential nutrients during the early developmental stages of aquatic species. This study aimed to evaluate the efficacy of synbiotic-enriched <i>Artemia franciscana</i> as a live feed for hybrid catfish larvae (<i>Clarias microstomus</i> × <i>Clarias gariepinus</i>), using locally isolated probiotics (<i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i>) and the commercial prebiotic inulin. The study was conducted in two phases. In Phase 1, <i>Artemia</i> were enriched for 6 h with four treatments: T1 (<i>E. faecium</i> W01 + inulin), T2 (<i>E. faecalis</i> + inulin), T3 (<i>E. faecium</i> W02 + inulin), and T4 (control and no synbiotics). Bacterial colonization was assessed microscopically and through colony counts at 2, 4, and 6 h post-enrichment. Synbiotic-treated groups (T1–T3) showed significantly higher bacterial retention than the control (T4), with T1 achieving the highest colonization levels (2 h: 6.98 log CFU/g; 4 h: 7.02 log CFU/g; 6 h: 7.10 log CFU/g; <i>p</i>  &lt; 0.05). Control values ranged from 5.12 to 5.45 log CFU/g. Microscopy revealed a distinctive red-brown gut coloration in treated <i>Artemia</i>, indicating successful colonization. In Phase 2, hybrid catfish larvae were fed enriched <i>Artemia</i> for 7 days, followed by a subsequent 7-day period transitioned to enriched <i>Artemia</i> and commercial pellet feed. T3 resulted in the highest weight gain (263.14 ± 34.70 mg), length gain (14.38 ± 5.10 mm), specific growth rate (SGR; 19.59 ± 1.17% day⁻¹), and the lowest feed conversion ratio (FCR; 0.10 ± 0.01), all significantly better than other treatments (<i>p</i>  &lt; 0.05). Although survival rates did not differ significantly, T3 recorded the highest survival (57.5 ± 3.81%). Forty presumptive lactic acid bacteria (LAB) were isolated from the fish gut, grouped into four main clusters. These results highlight the potential of synbiotic-enriched <i>Artemia</i> to enhance larval growth and gut health, offering an eco-friendly strategy to improve feed efficiency and reduce antibiotic dependance in aquaculture.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/6616288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Nanonutrient Complex on Growth Performance, Feed Efficiency, and Hematobiochemical Profiles in Asian Catfish, Clarias batrachus","authors":"Md. Ayenuddin Haque,&nbsp;Md. Abu Sayed Jewel,&nbsp;Nasrin Akter,&nbsp;Sumaiya Akter,&nbsp;Arun Chandra Barman,&nbsp;S. M. Nurul Amin,&nbsp;Takaomi Arai,&nbsp;Mohammed Fahad Albeshr,&nbsp;Norhayati Ngah,&nbsp;Mohammad Belal Hossain","doi":"10.1155/anu/7382715","DOIUrl":"https://doi.org/10.1155/anu/7382715","url":null,"abstract":"<p>Understanding how nanonutrients influence the growth and physiological processes of cultivable fish can boost fish production efficiency with less management, advancing aquaculture toward global food security. In this study, a 60-day feeding trial was conducted to determine the effects of a nanonutrient complex (NNC) on the growth performances and physiology of Asian catfish, <i>Clarias batrachus</i>. Nanoparticles (NPs; Zn, Cu, and Fe) were synthesized from their metallic salts using an established acoustic method and characterized via scanning electron microscopy. The NNC was formulated by mixing zinc NPs (Zn-NPs), copper NPs (Cu-NPs), and iron NPs (Fe-NPs) in a 40:20:40 ratio. In the experiment, a basal diet was supplemented with NNC at concentrations of 0.0 (control), 10, 20, 30, 40, and 50 mg/kg and fed to <i>C. batrachus</i> for 60 days to evaluate growth and physiological parameters (hematology, lipid, and enzyme profiles). The findings indicated that fish administered 30 mg/kg NNC achieved the highest final weight (FW; 25.73 ± 0.41 g), weight gain (WG; 386.67% ± 10.12%), average daily gain (ADG; 0.34 ± 0.01 g/fish/day), specific growth rate (SGR; 2.64 ± 0.03%/day), and enhanced feed conversion ratio (FCR; 1.24 ± 0.03), with statistically significant differences (<i>p</i>  &lt; 0.05) relative to the control group. Regression study determined the ideal NNC dosage range to be 30.19–30.26 mg/kg for growth and FCR results. Muscle composition enhanced at this level, with protein and fat content attaining 18.6% ± 0.3% and 6.8% ± 0.2%, respectively. Hematological indices reached their optimum at 30 mg/kg NNC, with red blood cell (RBC) count (3.62 ± 0.21 × 10⁶/mm³), hemoglobin (Hb; 9.83 ± 0.12 g/dL), and hematocrit (Hct; 26.31% ± 0.52%) greatly surpassing those of other treatments. Serum biochemical analysis indicated elevated total protein (4.79 ± 0.05 g/dL), albumin (1.55 ± 0.04 g/dL), and globulin (3.24 ± 0.02 g/dL) at 30 mg/kg, while undesirable elevations in stress markers—cholesterol (224.84 ± 1.10 mg/dL), alanine aminotransferase (ALT; 33.75 ± 0.39 U/L), and aspartate aminotransferase (AST; 40.29 ± 1.17 U/L)—were noted at 50 mg/kg, suggesting potential toxicity at increased dosages. Bioaccumulation of trace elements was most pronounced in the liver, with copper concentrations 11.15% greater than zinc and 1.09% higher than iron, while overall accumulation in the liver surpassed that in muscle and serum by 21.84% and 57.84%, respectively.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/7382715","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth Performance, Blood Chemistry, and Intestinal Bacterial Community of Florida Pompano (Trachinotus carolinus) Fed Different Levels of Corn Fermented Protein and Yeast Diets","authors":"Trinh H. V. Ngo,&nbsp;Marty Riche,&nbsp;Timothy J. Bruce,&nbsp;D. Allen Davis","doi":"10.1155/anu/8872997","DOIUrl":"https://doi.org/10.1155/anu/8872997","url":null,"abstract":"<p>Developing species-specific diets for Florida pompano (<i>Trachinotus carolinus</i>) requires understanding both nutritional needs and ingredient responses in practical formulations. Previous research has successfully reduced animal protein inclusion to ~15% by using solvent-extracted soybean meal (SBM) as the primary protein source. Further cost savings may be achieved by incorporating other low-cost alternatives, such as corn fermented protein (CFP), a new sustainable ingredient produced using Fluid Quip Technologies. A 12-week growth trial was conducted with juvenile Florida pompano (initial weight 6.08 ± 0.55 g) using a fishmeal-free basal diet. The basal diet contained poultry by-product meal (15% diet) and the SBM (52% diet) as primary protein sources. The SBM was then incrementally replaced with CFP (5%, 10%, and 20% diet) and <i>Saccharomyces cerevisiae</i> fermentation product (FSC) (2%) on an equal protein basis. All diets were formulated to be isonitrogenous (40% protein) and isolipidic (8% lipid), and fish were reared in a recirculating aquaculture system (RAS). Fish across all treatments exhibited similar growth metrics, including final weight (FW) (44.61–56.98 g), weight gain (WG) (557%–738%), and feed conversion ratio (FCR) (1.56–1.75), with no significant differences (<i>p</i>  &gt; 0.05). Blood parameters remained within healthy ranges, and microbiome analyses revealed stable gut bacterial diversity and composition among treatments. These results suggest that CFP can be used as a protein source in practice for Florida pompano without impairing growth, health status, or intestinal microbiota composition.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/8872997","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance, Nutrient Digestibility and Physiological Resilience of Juvenile Gilthead Seabream (Sparus aurata) Fed Organic and Circular Economy-Derived Diets","authors":"Rodrigo Mendes,&nbsp;Rita Teodósio,&nbsp;Jorge Dias,&nbsp;Ana Teresa Gonçalves,&nbsp;Lais Speranza,&nbsp;Sara Magalhães,&nbsp;Tiago Aires,&nbsp;Francisco J. Sánchez-Vázquez,&nbsp;Luís E. C. Conceição,&nbsp;Sofia Engrola","doi":"10.1155/anu/9559268","DOIUrl":"https://doi.org/10.1155/anu/9559268","url":null,"abstract":"<p>Aquafeeds formulated with organic or circular economy-derived ingredients aim to enhance sustainability and consumer acceptance. This study evaluated the global warming potential (GWP) and digestibility of such feeds, and assessed their effects on performance, feed utilisation and physiological resilience, defined as the ability to maintain tissue function and integrity under different feeding conditions of juvenile gilthead seabream (<i>Sparus aurata</i>) during grow out and after an overcrowding stress challenge. Three isonitrogenous (~51% crude protein) and isoenergetic (~18% crude fat) diets with limited fishmeal were formulated: a control (CTRL) commercial-like feed; an organic (ORG) diet based on organic-certified ingredients rich in plant proteins (primarily pea protein concentrate and wheat gluten); an eco-efficient (ECO) diet mainly composed of circular economy-derived animal by-products (e.g., poultry meal and feathermeal hydrolysate). The GWP was estimated using a life cycle assessment. Juvenile seabream (~14 g) were stocked in triplicate 500 L tanks (90 fish per tank, initial density of 2.5 kg/m³) and fed three times daily following feeding tables generated by FiT Feeding Tables, to optimise ration and minimise waste, over a 65-day growth period (final density of 8 kg/m³) and a subsequent 14-day overcrowding challenge (initial density of 12.4 kg/m³). At the end of the growth period, all groups exhibited at least a threefold increase in body weight. Feed digestibility was high (apparent digestibility coefficients (ADCs) &gt; 60%) and utilisation efficient. Physiological resilience was supported by stable growth and relative expression of biomarkers for gut health, oxidative status and immune function. Although ORG and ECO diets showed a higher GWP, this impact may decrease with increased use of renewable energy in ingredient production. The ORG diet also improved fish phosphorus retention. These organic and circular economy-derived feeds present viable options to reduce aquaculture’s environmental footprint while maintaining fish performance and resilience.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/9559268","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Buffer-Protected Sodium Butyrate Supplementation in the Diet of Penaeus vannamei","authors":"Maria Érica da Silva Oliveira,&nbsp;Joice Teixeira Souza,&nbsp;Ana Elidarly Cunha,&nbsp;Vanessa Maria Freitas Silva,&nbsp;Mário Augusto Monteiro Silva,&nbsp;Daniela Nomura Varandas,&nbsp;Andreia Vilas Boas,&nbsp;Ana Louise Toledo,&nbsp;Marisela Arturo Schaan,&nbsp;Javier Sánchez,&nbsp;Beatriz Saldaña,&nbsp;Moacir Franco de Oliveira,&nbsp;Matheus Ramalho de Lima","doi":"10.1155/anu/5578544","DOIUrl":"https://doi.org/10.1155/anu/5578544","url":null,"abstract":"<p>This study aimed to determine the optimal inclusion level of protected sodium butyrate (PSB) in diets for <i>Penaeus vannamei</i> during the postlarvae to grow-out phase. A completely randomized design was used with four dietary treatments: 0, 2, 4, and 8 kg/t of PSB (54% sodium butyrate). The PSB product (Novation SL 2002, Spain) contains 54% sodium butyrate protected by a physical and chemical matrix based on buffer salts. Growth performance, feed efficiency, survival, histological parameters of the intestine and hepatopancreas, and meat quality were evaluated. The results demonstrated that PSB supplementation significantly improved growth performance, feed conversion ratio (FCR), survival, and gut histomorphology, as well as enhanced meat production and quality. Based on the growth and feed efficiency responses, the optimal PSB inclusion level is recommended between 4 and 6 kg/t. Therefore, PSB is an effective dietary additive to improve productivity, gut health, and product quality in <i>P. vannamei</i> farming.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2025 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/5578544","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}