Aquaculture Nutrition最新文献

{"title":"Combined Substitution of Fishmeal and Fish Oil With Black Soldier Fly Larval Meal and Corn Oil: Effects on Growth, Hematology, Hematobiochemical, Amino and Fatty Acid Composition, and Fillet Nutritional Quality of Hybrid African Catfish Clarias gariepinus × Heterobranchus longifilis","authors":"Christopher Teye-Gaga,&nbsp;Péter István Molnár,&nbsp;Attila Kertész,&nbsp;John Kiguru Maina,&nbsp;Elshafia Ali Hamid Mohammed,&nbsp;Gabriella Gulyás,&nbsp;Levente Czeglédi,&nbsp;Milán Fehér,&nbsp;Péter Bársony","doi":"10.1155/anu/4880013","DOIUrl":"https://doi.org/10.1155/anu/4880013","url":null,"abstract":"<p>This study aimed to evaluate growth performance, amino acid and fatty acid composition of fillets, and blood biochemistry of hybrid catfish (<i>Heteroclarias</i>) cultured on Black Soldier Fly (BSF), <i>Hermetia illucens</i> larval-based diets. The experiment was conducted in a recirculating system, circular poly tanks (350 L), in a completely randomized design. Four isonitrogenous (400 g kg⁻¹ crude protein) and isolipidic (140 g kg⁻¹ crude fat) diets were formulated in which fishmeal (400 g kg⁻¹) was replaced at 0%, 25%, 50%, and 75% with defatted BSF larval meal and fish oil was completely replaced with corn oil in the test diets. 180 hybrid African catfish (12 weeks post-hatching) with an initial body weight of 200 ± 25 g were randomly distributed in the 12 experimental tanks (15 fish per tank, 45 fish per treatment) and were hand-fed at 3% body weight for 8 weeks. The findings showed that replacing 50% of fishmeal with BSF meal resulted in the highest growth performance (final weight, weight gain, specific growth rate [SGR], thermal growth coefficient [TGC]). However, at 75% level, growth performance and nutrient utilization (FCR, PER) significantly decline (<i>p</i>  &lt; 0.05). The dietary modification had no significant impact (<i>p</i>  &gt; 0.05) on organosomatic indices, proximate composition, amino acid profile, deposition, or retention of arachidonic acid (ARA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and sum n-3 polyunsaturated fatty acids (PUFA) in fish fillets. There were no significant differences in the hematological parameters (<i>p</i>  &gt; 0.05) across all treatments. Except for a reduction in globulin and cholesterol levels, all the plasma metabolites, including alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, remained stable (<i>p</i>  &gt; 0.05). Overall, the findings of this study suggest that BSF larval meal may partially replace dietary fishmeal up to 50% (200 g kg⁻¹), and corn oil may completely replace fish oil in a practical diet for hybrid African catfish without exerting adverse impacts on growth, feed conversion efficiency, fillet quality, health status, and physiological well-being.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/4880013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147715277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Method of Using Activated Sludge for Feed Modification in Aquaculture","authors":"Dmitry Rudoy,&nbsp;Irina Tkacheva,&nbsp;Elena Ponomareva,&nbsp;Anna Neidorf,&nbsp;Marina Oganisyan,&nbsp;Nadezhda Nedina","doi":"10.1155/anu/5549656","DOIUrl":"https://doi.org/10.1155/anu/5549656","url":null,"abstract":"<p>The article is devoted to the development and study of an innovative method for modifying feed in aquaculture based on the use of activated sludge from biofloc technologies (BFTs). The study presents the results of an experimental study of two BFT systems created on the basis of different complexes of bacterial strains: probiotic strains (S1) and strains isolated from the bottom sediments of the Don River (S2). The effectiveness of floc-forming bacteria strains MT141 and MT142 is demonstrated, which increased the biomass of flocs within 7 days and increased the content by 5%. The dominant bacteria in the BFT, out of the total number of species, were representatives of the genus <i>Runella</i> (up to 33%) and the genus Exiguobacterium (up to 26%), and 20% of the floc community bacteria were groups of the Christensenellaceae R-7 group, the genus <i>Acetoanaerobium</i>, and the genus <i>Bryobacter</i> (up to 11%). The amino acid composition of the active sludge proteins in the BFT system contained a significant percentage of amino acids, such as valine, glycine, and leucine, which were comparable to the requirements for their content in crustacean feed. The effectiveness of the feed additive was evaluated on two aquatic species, <i>Clarias gariepinus</i> and <i>Cherax quadricarinatus</i>. Catfish raised using the BFT system showed high biomass growth, with a survival rate of 98% and a two-fold reduction in feed conversion ratio. Australian red claw crayfish consuming the experimental feed increased their average daily growth by 0.2%, and the protein content in their muscle tissue was three times higher than in the control group. The results of the study confirm the promise of using dried activated sludge meal as a safe and effective protein supplement for aquaculture feed.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/5549656","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivating Microalgae With Pickled Cabbage Wastewater: A Sustainable Alternative to Replacing Fishmeal in Sea Urchin (Tripneustes gratilla) Diets","authors":"Sheng-Wei Ho,&nbsp;Hsiang-Yi Hsu,&nbsp;Yu-San Han,&nbsp;Mei-Ying Huang,&nbsp;Sheng-Yu Ho,&nbsp;Jen-Hong Chu","doi":"10.1155/anu/6673565","DOIUrl":"https://doi.org/10.1155/anu/6673565","url":null,"abstract":"<p>Recent studies have suggested that plant-based proteins, such as algal powder, can partially replace fishmeal used in formulated aqua feeds while maintaining the growth performance of aquaculture species. In Taiwan, the traditional pickled cabbage industry produces high-salinity wastewater rich in nutrients and microelements essential for plant growth. Reusing this nutrient-rich brine as a medium for microalgae cultivation offers a cost-effective and sustainable solution. This study aimed to evaluate the feasibility of using Taiwanese pickled cabbage wastewater (TPCW) to cultivate the halotolerant microalgae (<i>Dunaliella salina</i>), and of partially replacing fishmeal in <i>Tripneustes gratilla</i> (TG) feed with the resulting algal powder. The experiment consisted of two phases: (1) cultivation of <i>D. salina</i> using seawater partially substituted with varying proportions of TPCW and (2) assessment of the effects of replacing fishmeal with different levels of algal powder on the growth, survival, and gonad index of <i>T. gratilla</i> (0%: TG0, 30%: TG30, 35%: TG35, 40%: TG40, 45%: TG45, and 50%: TG50). Results showed that TPCW significantly enhanced the growth of <i>D. salina</i>, with the TPCW100 group (100% wastewater) displaying the highest growth rate, yielding 10.07 g of algal powder with 59.6% protein. Besides, a notable CO₂ decline in late culture stages indicated strong carbon uptake. For <i>T. gratilla</i>, the TG0 group exhibited the lowest weight gain and gonad index among all treatments, while TG40 showed the highest, significantly exceeding TG0 and TG30 (<i>p</i>  &lt; 0.05). Moreover, higher replacement levels of fishmeal with algal powder also increased gonad redness and yellowness, suggesting greater accumulation of pigments such as astaxanthin and carotenoids. Overall, this study confirms that agricultural waste can be effectively recycled for algal cultivation, contributing to carbon reduction, and that algal protein can successfully replace fishmeal in sea urchin feed, enhancing both growth and gonad coloration.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/6673565","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Arginine Regulates Glycometabolism in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) via the IGF-1 and NO-cGMP Pathways","authors":"Haoran Yin,&nbsp;Lixing Wang,&nbsp;Zexin Lin,&nbsp;Ruilin Li,&nbsp;Qinxiao Cai,&nbsp;Zhiyu Zhou,&nbsp;Xiaoyi Wu","doi":"10.1155/anu/4395252","DOIUrl":"https://doi.org/10.1155/anu/4395252","url":null,"abstract":"<p>The hybrid grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>Epinephelus lanceolatus</i> ♂), a typical carnivorous fish, has a limited ability to utilize dietary carbohydrates (CHOs). This study investigated the effects of different dietary arginine (Arg) levels (2.55 and 3.65%) and CHO-to-lipid ratios (C/L: 3.57, 2.39, 1.61) on the glycometabolism of hybrid grouper. Juvenile hybrid grouper (mean initial body length and weight: 7.8 cm and 13.04 g) were manually fed to fullness two times per day throughout a 7-week growth trial. The results showed that the C/L3.67 diets significantly elevated 2-h postprandial blood glucose concentration, as well as reduced hepatic glycogen level, intraperitoneal fat ratio (IPF), and the whole-body and hepatic lipid contents relative to the C/L2.39 and C/L1.61 diets. Dietary Arg supplementation significantly improved this metabolic disorder. These improvements were associated with enhanced plasma insulin-like growth factor 1 (IGF-1) concentration, hepatic phosphofructokinase (PFK) activity, nitric oxide (NO), and cyclic guanosine monophosphate (cGMP) levels. They were also linked to the upregulated expression of key molecules in related pathways, including IGF-1 receptor (igf1r), phosphatidylinositol 3-kinase (pi3k), protein kinase AMP-activated catalytic subunit α (ampk-α), and protein kinase B (Akt) genes, as well as glucose transporter 4 (GLUT4) and mechanistic target of rapamycin (mTOR) proteins. Generally, suitable dietary Arg supplementation can reduce postprandial blood glucose and hepatic glycogen concentrations in hybrid grouper fed high C/L diets by regulating key genes and proteins within the IGF-1 and NO-cGMP pathways. This finding provided a nutritional intervention to enhance CHO utilization in hybrid grouper aquaculture.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/4395252","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conjugated Bile Acids Enhance Growth Performance, Liver Health, and Bile Acid Metabolism in Pearl Gentian Grouper Fed a High Plant-Protein Diet","authors":"Fan Wang,&nbsp;Fajun Jiang,&nbsp;Jia Xu,&nbsp;Yixin Liang,&nbsp;Xujia Liu,&nbsp;Yichao Li,&nbsp;Junlian Zhuang,&nbsp;Fangjuan Huang,&nbsp;Fu Lei,&nbsp;Chuhang Chen,&nbsp;Ermeng Yu,&nbsp;Junxiang Lai","doi":"10.1155/anu/1857237","DOIUrl":"https://doi.org/10.1155/anu/1857237","url":null,"abstract":"<p>The conjugation states of bile acids (BAs) have been demonstrated to affect BAs regulatory functions. This study aimed to investigate the effects of conjugated or non-conjugated BA additions on growth performance, liver health, and BA metabolism in pearl gentian grouper (<i>Epinephelus lanceolatus</i>♂ × <i>E. fuscoguttatus</i>♀), as well as to elucidate the underlying mechanisms. The 300 healthy experimental fish, each with an average initial body weight (IBW) of 15.21 ± 0.04 g, were randomly distributed into 12 tanks (300 L per tank). The experiment consisted of four dietary groups, with three replicates per group: the control diet (CD) group, the plant protein-based diet (PD) group, the PD supplemented with conjugated BAs (CBD) group, and the PD supplemented with non-conjugated BA diet (NBD) group. The findings indicated that, in comparison to the CD group, the grouper fed a PD exhibited deleterious effects on growth performance, BA metabolism, and liver health. This was evidenced by increased levels of of oxidative stress, endoplasmic reticulum (ER) stress, apoptosis, and inflammatory response. In comparison to the PD group, the CBD treatment demonstrated favorable outcomes with respect to growth performance and liver health in the grouper. Additionally, it facilitated BA metabolism, as evidenced by enhanced BA transport, reabsorption, and recycling. Compared to PD group, the NBD treatment further impaired BA metabolism and induced cell apoptosis. Additionally, six metabolites, 3-Dehydrocarnitine, Stizolobate, Bilastine, S-(-)-Ureidoglycolate, 5,6-Dihydroxyindole, and 4-O-methylnorbelladine-, were identified as biomarkers among the treatment groups. Moreover, high plant-protein replacement and BAs addition both impacted the pathways associated with the “Biosynthesis of various plant secondary metabolites“ and “Central carbon metabolism in cancer. “In conclusion, the conjugated BAs improve growth performance, liver health, and BA metabolism in pearl gentian grouper fed a high plant-protein diet.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/1857237","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147568132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Protein and Lipid Levels Affect Growth Performance, Antioxidant Capacity, and Metabolism in Postlarval Macrobrachium rosenbergii","authors":"Xiaochuan Zheng,&nbsp;Yunke Liu,&nbsp;Ling Zhu,&nbsp;Cunxin Sun,&nbsp;Aimin Wang,&nbsp;Jianming Chen,&nbsp;Xiaojun Jing,&nbsp;Bo Liu","doi":"10.1155/anu/9480329","DOIUrl":"https://doi.org/10.1155/anu/9480329","url":null,"abstract":"<p>The protein-to-energy ratio is crucial for the overall performance of <i>Macrobrachium rosenbergii</i> postlarvae, yet the optimal range remains undetermined. We formulated nine experimental diets in a 3 × 3 factorial design, encompassing three graded protein levels (45.83%, P46; 49.03%, P49; 52.23%, P52) and three graded lipid levels (6.07%, L6; 8.03%, L8; 10.03%, L10). After an 8-week feeding trial, the weight gain rate (WGR) was influenced by both protein and lipid levels, with a peak in the P49/L8 group (<i>p</i> &lt; 0.05). Their interaction significantly affected hemolymph superoxide dismutase (SOD) and malondialdehyde (MDA) levels (<i>p</i> &lt; 0.05). SOD initially increased and then decreased with increasing protein level, peaking in the P49/L8 group (<i>p</i> &lt; 0.05). Total antioxidant capacity (T-AOC) decreased with increasing lipid level but showed an initial increase followed by a decrease at the P52 level, reaching its highest value in the P52/L8 group (<i>p</i> &lt; 0.05). At low lipid levels, trypsin increased with increasing protein, whereas it decreased at high lipid levels. Lipase initially increased and then decreased with increasing levels of both protein and lipid, reaching its peak in the P52/L8 group (<i>p</i> &lt; 0.05). The relative lipid droplet area increased with increasing protein and lipid levels, reaching a maximum in the P52/L10 group (<i>p</i> &lt; 0.05). Appropriate protein level significantly activated the <i>TOR</i>/<i>S6K1</i> pathway, while elevated lipid levels upregulated genes related to lipid synthesis (<i>FAS</i> and <i>ACC</i>) and transport (fatty acid-binding protein [<i>FABP</i>]), while downregulating genes associated with lipid breakdown. In conclusion, the optimal dietary protein and lipid levels for postlarval <i>M. rosenbergii</i> are 49% and 8%, respectively, corresponding to a P/E ratio of 29.34 mg protein·kJ⁻¹.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/9480329","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147568133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response Characteristics of Defatted Mealworm (Tenebrio molitor) Instead of Fish Meal to the Intestinal Immunity and Microflora of Juvenile Pikeperch (Sander lucioperca)","authors":"Zhipeng Sun,&nbsp;Jie Zhao,&nbsp;Liansheng Wang,&nbsp;Ze Fan,&nbsp;Tianqi Liu,&nbsp;Kexin Zhang,&nbsp;Rongbin Na,&nbsp;Cuiyun Lu,&nbsp;Xianhu Zheng","doi":"10.1155/anu/5253366","DOIUrl":"https://doi.org/10.1155/anu/5253366","url":null,"abstract":"<p>The aim of this study was to investigate the impact of varying levels of defatted mealworm as a substitute for fish meal on the growth, intestinal health, and intestinal microflora of juvenile pikeperch (<i>Sander lucioperca</i>). Juveniles were fed diets containing 54% protein and 12% lipid over an 8-week period, with defatted mealworms replacing 0%, 25%, 50%, 75%, and 100% of fish meal, respectively (designated as MW0, MW25, MW50, MW75, and MW100). The results showed that the MW25 group achieved the highest weight gain rate and specific growth rate, with no significant difference from the MW0 group. Moreover, the MW25 group exhibited the lowest feed conversion ratio. This group also exhibited the highest trypsin activity, whereas lipase activity showed no significant variation across all treatment groups. Intestinal villus height and width were not significantly affected by mealworm inclusion. These findings suggest that the inclusion of mealworm did not impact the digestive and absorptive capacity of the pikeperch intestine. However, gene expression related to growth (<i>ghr</i>, <i>igf1</i>, <i>igf2</i>) showed a consistent decline as mealworm inclusion increased (<i>p</i> &lt; 0.05). The expression of <i>il10</i> was highest in the MW25 group (<i>p</i> &lt; 0.05), whereas <i>il-1β</i> and <i>tgf-β</i> expressions peaked in the MW50 group. Additionally, increasing mealworm levels led to a reduction in intestinal microflora diversity and abundance, with a decreased presence of <i>Staphylococcus</i> and <i>Lactobacillus</i>, which negatively affected the intestinal microflora structure. In conclusion, replacing no more than 25% of fish meal with mealworm is recommended to optimize growth performance and preserve intestinal microflora structure in juvenile pikeperch.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/5253366","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Berberine Improves Liver and Intestinal Health for Hybrid Yellow Catfish (Pelteobagrus fulvidraco ♀ × Pelteobagrus vachelli ♂) Suffered Dual Stress From Soybean Meal-Induced Enteritis and Lipopolysaccharide","authors":"Jiale Hong,&nbsp;YuJie Zhi,&nbsp;Xiaobo Wang,&nbsp;Zhengyang Wu,&nbsp;Jiayi Chen,&nbsp;Meina Zhang,&nbsp;ChengJia Wu,&nbsp;Xiaocheng Huang,&nbsp;Ningning Su,&nbsp;Guangqing Yu,&nbsp;Pengsheng Dong,&nbsp;Zhenjiang Yang,&nbsp;Guojun Guo,&nbsp;Guoxi Li,&nbsp;Ming Li,&nbsp;Wen Chen,&nbsp;Bianzhi Liu","doi":"10.1155/anu/2606502","DOIUrl":"https://doi.org/10.1155/anu/2606502","url":null,"abstract":"<p>In order to investigate the effect of dietary berberine (BBR) on lipopolysaccharide (LPS) stimulation in hybrid yellow catfish with soybean meal-induced enteritis (SBMIE), two diets were formulated: a control diet containing 33.75% soybean meal (SBM) and an experimental diet with 33.75% SBM supplemented with 75 mg/kg BBR. After 42 days of feeding, the fish from each dietary group were divided into two subgroups, one of which was subsequently challenged with LPS, while the other was not. Then, liver and hindgut tissues were sampled at 12 and 24 h postinfection, respectively, to analyze the oxidative stress, antioxidant capacity, histopathology, immune response, and apoptosis. The results revealed that dietary SBM of 33.75% significantly induced oxidative stress, histopathological damage, immune dysfunction, and apoptosis in both the liver and hindgut of hybrid yellow catfish. Furthermore, exposure to LPS exacerbated these symptoms. In contrast, 75 mg/kg BBR in the diet significantly improved liver and hindgut health via enhancing oxidative stress resistance, antioxidative capacity, and the intestinal barrier function, without affecting growth or feed utilization. Also, it effectively protected liver and intestinal health from LPS damage. The subgroup infected with LPS in the dietary BBR group showed marked improvements in all assessed parameters, including oxidative stress, antioxidant capacity, histopathology damage, immune dysfunction, liver apoptosis, and intestinal physical barrier, when compared with those in the subgroup infected with LPS in the control group. In conclusion, BBR represents a promising feed additive for enhancing fish health under combined stress from SBM and LPS-induced stress.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/2606502","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emodin Improved the Hepatic Metabolic Dysfunction Induced by High Plant-Protein Diet in Pengze Crucian Carp (Carassius auratus var. Pengze)","authors":"Kaikai Shen,&nbsp;Yongfa Huang,&nbsp;Zhenwei Lu,&nbsp;Jianqin Li,&nbsp;Jingjie Zhang,&nbsp;Ao Luo,&nbsp;Baoqing Hu,&nbsp;Jingen Xu,&nbsp;Jiamin Li,&nbsp;Chungen Wen,&nbsp;Gang Yang","doi":"10.1155/anu/4612787","DOIUrl":"https://doi.org/10.1155/anu/4612787","url":null,"abstract":"<p>High plant-protein (HP) diets could induce bile acid (BA) imbalance and lipid metabolism disorder in fish, and emodin supplementation has been reported to increase serum BA levels and improve lipid metabolism with a lack of understanding of its underlying mechanism. To explore this scientific problem, Pengze crucian carp were respectively fed with normal fishmeal (FM) diet, HP diet, and HP diet with different levels of emodin (25, 50, 75, and 100 mg/kg) for 8 weeks. The results revealed that appropriate emodin supplementation recovered intestinal BAs absorption function through the inhibition of intestinal inflammation and promoted the hepatic BAs synthesis by inhibiting farnesoid X receptor (FXR)-FGF19-CYP7A1 signaling pathway in carp fed with HP diet. Emodin exerted cholesterol (CHOL)-lowering effects by inhibiting the excessive synthesis of CHOL induced by HP diet and promoting the transport of CHOL, and drived the conversion of CHOL to BAs in liver tissue. The increase in BA levels induced by emodin supplementation could suppress lipid lipogenesis and alleviate hepatic lipid deposition through the activation of AMP-activated protein kinase (AMPK). In summary, dietary a suitable content of emodin could improve the metabolism of BA, CHOL, and lipid in liver tissue, and ultimately attenuate hepatic metabolic dysfunction of Pengze crucian carp induced by HP diet.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/4612787","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Endogenous Probiotics Enhance Host Intestinal Health, Liver Immune Capacity, Meat Nutritional Composition, and Aquatic Environmental Quality in Pelodiscus sinensis","authors":"Yuhong Liao,&nbsp;Hao Fu,&nbsp;Ganlin Lv,&nbsp;Huihui Quan,&nbsp;Qing Qiu,&nbsp;Huahua Mao,&nbsp;Jubin Xing,&nbsp;Zirui Wang,&nbsp;Qingman Yang,&nbsp;Xionge Pi","doi":"10.1155/anu/7893677","DOIUrl":"https://doi.org/10.1155/anu/7893677","url":null,"abstract":"<p>Currently, the in-depth research and practical application of probiotics in the green and healthy breeding of Chinese soft-shelled turtles (<i>Pelodiscus sinensis</i>) remain limited. This study conducted a 30-day feeding trial with turtles of an initial weight of 528.95 ± 2.96 g. The aim was to evaluate the effects of three endogenous probiotics—<i>Enterococcus faecalis</i> (EF), <i>Lactococcus lactis</i> (LL), and <i>Weissella cibaria</i> (WC) on the intestinal health, liver antioxidant capacity, muscle nutritional components, intestinal microbiota composition, and aquaculture water quality of <i>P. sinensis</i>. Supplementation with probiotics significantly reshaped the microbial communities in the gut and aquaculture water, enriching beneficial bacterial genera such as <i>Romboutsia</i> and <i>Bacteroides</i>. Strain-specific functional benefits were observed: LL significantly improved villus height (VH) and lamina propria thickness (<i>p</i> &lt; 0.05); WC significantly enhanced liver antioxidant enzyme activities. including glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT), as well as the content of essential amino acids (EAAs) in muscle (<i>p</i> &lt; 0.05); EF significantly increased trypsin activity and the content of umami amino acids (UAAs) in muscle (<i>p</i> &lt; 0.05), while enriching beneficial bacteria such as <i>Nitrosomonas</i> and improving water quality by reducing total phosphorus and ammonia nitrogen. Correlation analysis linked specific microbial taxa to host physiological and environmental parameters. These findings demonstrate the diverse and unique roles of endogenous probiotics and provide a scientific basis for their precise application in sustainable turtle farming.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2026 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/anu/7893677","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}