Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed Meal

IF 3.9 2区农林科学 Q1 FISHERIES

Aquaculture Nutrition Pub Date : 2025-07-30 DOI:10.1155/anu/3661772

Hong-yu Lu, An-Ning Shi, Jun Wang, Li-Juan Liu, Jiang-Yi Tang, Ya-Jun Wang, Xue-Zhi Zhu, De-Feng Zhang, Li-Yan Liu, Qing Wang

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Abstract

Glucoside is the main antinutritional factor (ANF) of rapeseed meal. In the present study, a bacterium with high efficiency in degrading rapeseed glucoside was screened from pond water. The initial concentration of 1.0 × 10⁷ CFU/mL bacterial solution was added to rapeseed meal and fermented for 48 h with a degradation rate of glucoside and isothiocyanate of 67% and 54%, respectively. BSY82 was identified by PacBio third-generation sequencing, and its biology was analyzed. Results showed that BSY82 belongs to Bacillus subtilis. The total length of genome was 4,242,094 bp, among which 89% was coding genes (3,777,298 bp). No virulence factors were predicted, based on a comparative analysis of the strain’s coding genes against known virulence factor databases. Functional analysis showed that BSY82 had the ability to produce myrosinase. The activity of myrosinase in the crude enzyme solution of the strain was 1.67 and 2.12 μmol/min by spectrophotometry and high-performance liquid chromatography (HPLC). The results indicated that BSY82 strain could be used as the optimal material in rapeseed meal fermentation detoxification process.

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菜籽粕中枯草芽孢杆菌BSY82的生物学特性及硫代葡萄糖苷降解机理

糖苷是菜籽粕中主要的抗营养因子。本研究从池塘水中筛选出一种高效降解油菜籽糖苷的细菌。将初始浓度为1.0 × 107 CFU/mL的细菌溶液加入到菜籽粕中，发酵48 h，对葡萄糖苷和异硫氰酸酯的降解率分别为67%和54%。通过PacBio第三代测序对BSY82进行鉴定，并对其进行生物学分析。结果表明，BSY82属枯草芽孢杆菌。基因组总长度为4,242,094 bp，其中编码基因占89% （3,777,298 bp）。根据菌株编码基因与已知毒力因子数据库的比较分析，没有预测毒力因子。功能分析表明，BSY82具有产生黑芥子酶的能力。分光光度法和高效液相色谱法测定菌株粗酶液中黑芥子酶活性分别为1.67和2.12 μmol/min。结果表明，菌株BSY82可作为油菜籽粕发酵脱毒工艺的最佳材料。

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来源期刊

Aquaculture Nutrition 农林科学-渔业

CiteScore

7.20

自引率

8.60%

发文量

131

审稿时长

3 months

期刊介绍： Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers. Aquaculture Nutrition publishes papers which strive to: increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research. improve understanding of the relationships between nutrition and the environmental impact of aquaculture. increase understanding of the relationships between nutrition and processing, product quality, and the consumer. help aquaculturalists improve their management and understanding of the complex discipline of nutrition. help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.