Allergologia et immunopathologia最新文献

{"title":"Knockdown of TRIM22 regulates the expression of NF-κB/NLRP3 and alleviates inflammation and renal injury in mice with lupus nephritis.","authors":"Jie Chen, Lu Liu, Min Liu, Mingming Qin, Suyan Yan, Chunfeng Wu, Wenjie Yu","doi":"10.15586/aei.v53i3.1313","DOIUrl":"https://doi.org/10.15586/aei.v53i3.1313","url":null,"abstract":"<p><p>Lupus nephritis (LN) is a common and clinically challenging complication of the systemic lupus erythematosus (SLE), but effective treatments remain imperative. Tripartite Motif Containing 22 (TRIM22) is a protein involved in various cellular processes, such as cell growth and inflammatory responses. However, the role and mechanism of TRIM22 in LN are still unclear. The aim of this study was to reveal the role of TRIM22 in LN and uncover the potential mechanisms. We discovered that TRIM22 is notably upregulated in renal tissues from patients with LN. Functionally, the knockdown of TRIM22 in lupus-prone MRL/lpr mice results in significant alleviation of LN symptoms, characterized by reduced proteinuria and improved renal function, as indicated by the lower serum levels of blood urea nitrogen and creatinine. Additionally, the intervention markedly decreases the deposition of immune complexes in the kidneys, further supporting the therapeutic potential of targeting TRIM22. Mechanically, the depletion of TRIM22 inhibits the NF-κB/NLRP3 pathway in mice with LN, but collectively, the knockdown of TRIM22 alleviates inflammation and renal injury in mice with LN via the NF-κB/NLRP3 axis.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 3","pages":"98-105"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaphylaxis due to green beans (<i>Phaseolus vulgaris</i>): A new phenotype?","authors":"Estela Menéndez Rivero, Manuel Prados Castaño, Isabel María Domínguez Romero, María José Martínez Martínez, Fernando Pineda De La Losa","doi":"10.15586/aei.v53i3.1368","DOIUrl":"https://doi.org/10.15586/aei.v53i3.1368","url":null,"abstract":"","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 3","pages":"206-207"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corticosteroid prescription in children with respiratory symptoms: A real-life study.","authors":"Remedios Cárdenas Contreras, Alicia Habernau Mena, Elisa Haroun Díaz, Javier Ruíz Hornillos, Margarita Tomás Pérez, Rafael Pineda Pineda, Elena Mederos Luis, Maria Ángeles González Labrador, Rosadoi Ana, Silvia Sánchez García, Eloísa Rubio Pérez, Ignacio Salamanca de la Cueva","doi":"10.15586/aei.v53i3.1277","DOIUrl":"10.15586/aei.v53i3.1277","url":null,"abstract":"<p><p>Asthma is one of the most common childhood diseases. Most children respond to low doses of corticosteroids and/or anti-leukotrienes. However, a small percentage remain symptomatic. They require high doses of inhaled corticosteroids (ICS) and anti-leukotrienes, sometimes with long-acting bronchodilators (LABA) and occasionally systemic corticosteroids (SC). However, we frequently observe that the only treatment they receive for exacerbations is short-acting beta-agonists (SABA) and oral corticosteroids (OC) without adding ICS as a maintenance treatment to prevent new episodes. This leads to frequent exacerbations, airflow limitation, and significant alterations in the quality of life. In addition, this results in high resource consumption, frequent emergency room visits, and school absenteeism, leading to absence from work for caregivers. A descriptive, retrospective, observational, and multicenter study was carried out on patients under 12 years of age, who attended pediatric allergy consultations for the first time. The main objective was to determine the consumption of SCs because of respiratory exacerbations in the year before the first consultation. Data were obtained from 144 children, 58.3% male, with a median age of 5 years. It was found that during the year before attending our clinic, 70% had perennial symptoms and were only prescribed with salbutamol on demand when they presented with respiratory symptoms. They had not been prescribed with ICS or anti-leukotrienes. They required attention in the emergency room of their nearest health center or hospital on an average of three times per year. During these exacerbations, almost 75% received SCs(prednisolone), with an average of 2.6 annual cycles (0-12) prescribed in more than half of the cases in the emergency room. In over 80% of the cases, the effects of SC abuse were not previously monitored. The overuse of SC in atopic children with respiratory symptoms was confirmed in our group, which requires considering behavioral changes.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 3","pages":"115-121"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DUOX1 inhibits the progression of rheumatoid arthritis by regulating the NF-κB pathway in vitro.","authors":"Dan Xuan, Dandan Feng, Fuyong Qiang, Yonghui Xia","doi":"10.15586/aei.v53i2.1293","DOIUrl":"10.15586/aei.v53i2.1293","url":null,"abstract":"<p><strong>Background: </strong>This study investigates the role of dual oxidase 2 (DUOX1) in fibroblast-like synoviocytes associated with rheumatoid arthritis (RA) and to elucidate its potential mechanism of action.</p><p><strong>Method: </strong>The anti-inflammatory effects of DUOX1 were assessed using IL-1β (interleukin-1 beta)-stimulated synovial fibroblasts (MH7A). Cell viability and migration were evaluated using the Cell Counting Kit-8 and Transwell assays, respectively. Enzyme-linked immunosorbent assay (ELISA) was performed to measure cellular inflammatory factor levels, and immunofluorescence and specific kits were used to assess reactive oxygen species (ROS) production and redox indicators. Western blotting was performed to confirm the antiarthritic mechanism of DUOX1.</p><p><strong>Result: </strong>The findings revealed that the stimulation if IL-1β downregulates DUOX1 expression in MH7A cells, leading to increased proliferation, migration, inflammatory responses, and oxidative stress. Conversely, DUOX1 overexpression increased the production of IL-1β inducing excessive proliferation, migration, inflammation, and oxidative stress in MH7A cells, and inhibited the activation of the nuclear factor kappa B (NF-κB) inflammatory pathway.</p><p><strong>Conclusion: </strong>DUOX1 significantly suppresses the proliferation, migration, inflammation, and oxidative stress of RA synovial cells through the inhibition of the NF-κB signaling pathway.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"160-168"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of ANXA3 regulates NF-κB/STAT3 pathway to alleviate inflammation and hyperproliferation in psoriasis models.","authors":"Jin Li, Fang Ren, Hongshan Yuan, Wenliang Yan","doi":"10.15586/aei.v53i2.1260","DOIUrl":"10.15586/aei.v53i2.1260","url":null,"abstract":"<p><p>Psoriasis is an immune-mediated inflammatory skin disorder and its pathological mechanism remains incompletely understood. Detailed exploration of this mechanism is crucial to identify key regulatory molecules influencing its progression. In previous studies, Annexin A3 (ANXA3), a calcium-dependent phospholipid-binding protein from the annexin family, has been linked to psoriasis progression. However, its specific effects on the disease remain unclear. This study aimed to investigate the role of ANXA3 in psoriasis progression. For this purpose, we employed an imiquimod (IMQ)-induced mouse model and <i>in-vitro</i> experiments to uncover the underlying cellular mechanisms. A mixture of five inflammatory factors (TNF-α, IL-1α, IL-17A, IL-22, and statin M) was used to stimulate HaCaT cells, mimicking the psoriasis microenvironment. Our findings demonstrate that ANXA3 is highly expressed in psoriatic skin, and its knockdown alleviates skin lesions in IMQ-induced mice. Further analysis revealed that ANXA3 knockdown reduces skin tissue hyperplasia and decreases the expression of inflammatory factors in IMQ mice. Mechanistically, ANXA3 knockdown inhibits the NF-κB/STAT3 pathway in skin tissue. Additionally, ANXA3 knockdown inhibits inflammation and hyperproliferation in HaCaT cells. Collectively, these results indicate that ANXA3 alleviates psoriasis progression both <i>in-vivo</i> and <i>in-vitro</i> by inhibiting the NF-κB/STAT3 pathway.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"32-41"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food additives and their impact on human health.","authors":"Marilyn Urrutia-Pereira, Guillhermo Guidos Fogelbach, Herberto José Chong-Neto, Dirceu Solé","doi":"10.15586/aei.v53i2.1149","DOIUrl":"10.15586/aei.v53i2.1149","url":null,"abstract":"<p><p>Increasing evidence suggests that high consumption of ultra-processed foods (UPF) is associated with an increase in noncommunicable diseases, overweight, and obesity. This review aimed to verify the association of UPF with inflammatory diseases, especially allergic diseases. To identify relevant articles, an extensive literature search was conducted using the two most important search sites - PubMed and Google Scholar. Specific Medical Subject Headings (MeSHes) such as \"food additives and health,\" \"food additives and immune system,\" and \"food additives and diseases\" were used to conduct an advanced search. Emulsifiers have been, particularly, implicated in disrupting intestinal barrier function, modifying gut microbiota, and promoting inflammation, which may contribute to the development of food allergies and inflammatory diseases. While food additives serve various functions in the food industry, concerns regarding their impact on health, particularly in systemic autoimmune and metabolic conditions, have been raised. Common additives have been associated with allergic reactions, intolerances, and sensitivities.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"26-31"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug-induced enterocolitis, a new condition under consideration.","authors":"Lucía Hernando Marín, Ana María Segura Machado, Marta Castro Ramos, Javier Torres-Borrego","doi":"10.15586/aei.v53i2.1314","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1314","url":null,"abstract":"","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"50-51"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Idiopathic anaphylaxis due to SARS-CoV-2 mRNA vaccine?","authors":"Öner Özdemir","doi":"10.15586/aei.v53i2.1299","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1299","url":null,"abstract":"","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"8-9"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Echinocystic acid activates PPARγ to alleviate mannan-induced psoriasis and psoriatic arthritis in mice.","authors":"Chengwei Yu, Huiming Wu, Dongrui Zhao, Huajie Shi","doi":"10.15586/aei.v53i2.1283","DOIUrl":"10.15586/aei.v53i2.1283","url":null,"abstract":"<p><p>Previous studies have shown that echinocystic acid (EA) can reduce arthritis and skin damage, but the role of EA in psoriatic arthritis is unclear. This study aims to prove the role of EA in psoriatic arthritis, which was induced by intraperitoneal injection of mannan in C57BL/6J mice. The mice were divided into a control group, mannan group, mannan + EA (low-dose) group, and mannan + EA (high-dose) group. Joint tissue damage was scored, and pathological changes in joint tissue and ear skin damage were examined by HE staining. Pathway enrichment of EA drug targets was performed through the target enrichment website, and the mRNA and protein expression levels of pathway-related proteins in joint tissues and ears were verified using the PCR and western blot. The results show that injection of mannan into mice resulted in joint inflammatory infiltration and tissue damage, hyperkeratosis, and acanthosis of the ear skin, while these symptoms were alleviated after high-dose EA treatment. Pathway enrichment analysis showed that the EA drug treatment target is concentrated on the PPAR pathway. The mRNA and protein results showed that the mRNA and protein expression levels of peroxisome proliferator-activated receptor γ (PPARγ) in the joint tissues and ears of mice with psoriatic arthritis decreased, and the expression of PPARγ was activated after high-dose EA treatment. In conclusion, EA increases PPARγ expression and reduces joint and skin damage in mice with psoriatic arthritis.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"52-58"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is hen's egg a significant allergen in adults too? Findings from an investigation into food allergy prevalence.","authors":"Betül Özdel Öztürk, Begüm Görgülü Akın, Makbule Seda Bayrak Durmaz, Şadan Soyyiğit","doi":"10.15586/aei.v53i2.1263","DOIUrl":"10.15586/aei.v53i2.1263","url":null,"abstract":"<p><strong>Introduction: </strong>Data on adult food allergies (FAs) remain limited compared to the pediatric population, and this study investigates the prevalence and clinical characteristics of FAs among adults.</p><p><strong>Methods: </strong>This retrospective study included adults aged 18-80 diagnosed with FAs at the Immunology and Allergy Diseases Clinic of Ankara Bilkent City Hospital, Turkey, between 2019 and 2024.</p><p><strong>Results: </strong>During the study period, 10,505 patients were examined to diagnose and manage various allergic and immunologic conditions. Among these, 563 patients were suspected of having FAs, which was confirmed in 33 patients (mean age 34.74 ± 12.57 years; 60.6% female), yielding a frequency of 0.31%. The primary allergens identified included hen's egg (n = 6, 18.2%), fish and shellfish (n = 4, 12.1%), and various nuts such as peanuts (n = 2, 6.1%), hazelnut (n = 1, 3.0%), and almond (n = 1, 3.0%) as well as seeds like sesame (n = 2, 6.1%) and sweet corn (n = 2, 6.1%) and fruits such as banana (n = 4, 12.1%) and kiwi (n = 4, 12.1%). The median time interval between food consumption and allergic reactions was 10 minutes (2-120). Anaphylaxis was the most frequently observed clinical presentation (n = 24, 72.7%). Emergency treatments included antihistamines and steroids (n = 15, 65.2%), antihistamines, steroids, and epinephrine (n = 5, 21.7%), or epinephrine alone (n = 3, 13.0%). Egg allergy was the most prevalent allergen (n = 6, 18.2%), characterized by a rapid onset of allergic reactions (median of 5 minutes); two patients reacted regardless of cooking methods and four showed tolerance to thoroughly cooked eggs, with component-resolved diagnostics indicating sensitization to ovalbumin (Gal d2) but not ovomucoid (Gal d1).</p><p><strong>Conclusion: </strong>Our study demonstrates a low frequency of FAs in adults yet highlights the seriousness of reactions like anaphylaxis. Hen's egg allergy was notably prevalent, underscoring the need for increased awareness and improved diagnostic strategies to effectively manage FAs in the adult population.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"13-23"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}