Allergologia et immunopathologia最新文献

{"title":"DUOX1 inhibits the progression of rheumatoid arthritis by regulating the NF-κB pathway in vitro.","authors":"Dan Xuan, Dandan Feng, Fuyong Qiang, Yonghui Xia","doi":"10.15586/aei.v53i2.1293","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1293","url":null,"abstract":"<p><strong>Background: </strong>This study investigates the role of dual oxidase 2 (DUOX1) in fibroblast-like synoviocytes associated with rheumatoid arthritis (RA) and to elucidate its potential mechanism of action.</p><p><strong>Method: </strong>The anti-inflammatory effects of DUOX1 were assessed using IL-1β (interleukin-1 beta)-stimulated synovial fibroblasts (MH7A). Cell viability and migration were evaluated using the Cell Counting Kit-8 and Transwell assays, respectively. Enzyme-linked immunosorbent assay (ELISA) was performed to measure cellular inflammatory factor levels, and immunofluorescence and specific kits were used to assess reactive oxygen species (ROS) production and redox indicators. Western blotting was performed to confirm the antiarthritic mechanism of DUOX1.</p><p><strong>Result: </strong>The findings revealed that the stimulation if IL-1β downregulates DUOX1 expression in MH7A cells, leading to increased proliferation, migration, inflammatory responses, and oxidative stress. Conversely, DUOX1 overexpression increased the production of IL-1β inducing excessive proliferation, migration, inflammation, and oxidative stress in MH7A cells, and inhibited the activation of the nuclear factor kappa B (NF-κB) inflammatory pathway.</p><p><strong>Conclusion: </strong>DUOX1 significantly suppresses the proliferation, migration, inflammation, and oxidative stress of RA synovial cells through the inhibition of the NF-κB signaling pathway.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"160-168"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food additives and their impact on human health.","authors":"Marilyn Urrutia-Pereira, Guillhermo Guidos Fogelbach, Herberto José Chong-Neto, Dirceu Solé","doi":"10.15586/aei.v53i2.1149","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1149","url":null,"abstract":"<p><p>Increasing evidence suggests that high consumption of ultra-processed foods (UPF) is associated with an increase in noncommunicable diseases, overweight, and obesity. This review aimed to verify the association of UPF with inflammatory diseases, especially allergic diseases. To identify relevant articles, an extensive literature search was conducted using the two most important search sites - PubMed and Google Scholar. Specific Medical Subject Headings (MeSHes) such as \"food additives and health,\" \"food additives and immune system,\" and \"food additives and diseases\" were used to conduct an advanced search. Emulsifiers have been, particularly, implicated in disrupting intestinal barrier function, modifying gut microbiota, and promoting inflammation, which may contribute to the development of food allergies and inflammatory diseases. While food additives serve various functions in the food industry, concerns regarding their impact on health, particularly in systemic autoimmune and metabolic conditions, have been raised. Common additives have been associated with allergic reactions, intolerances, and sensitivities.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"26-31"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of ANXA3 regulates NF-κB/STAT3 pathway to alleviate inflammation and hyperproliferation in psoriasis models.","authors":"Jin Li, Fang Ren, Hongshan Yuan, Wenliang Yan","doi":"10.15586/aei.v53i2.1260","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1260","url":null,"abstract":"<p><p>Psoriasis is an immune-mediated inflammatory skin disorder and its pathological mechanism remains incompletely understood. Detailed exploration of this mechanism is crucial to identify key regulatory molecules influencing its progression. In previous studies, Annexin A3 (ANXA3), a calcium-dependent phospholipid-binding protein from the annexin family, has been linked to psoriasis progression. However, its specific effects on the disease remain unclear. This study aimed to investigate the role of ANXA3 in psoriasis progression. For this purpose, we employed an imiquimod (IMQ)-induced mouse model and <i>in-vitro</i> experiments to uncover the underlying cellular mechanisms. A mixture of five inflammatory factors (TNF-α, IL-1α, IL-17A, IL-22, and statin M) was used to stimulate HaCaT cells, mimicking the psoriasis microenvironment. Our findings demonstrate that ANXA3 is highly expressed in psoriatic skin, and its knockdown alleviates skin lesions in IMQ-induced mice. Further analysis revealed that ANXA3 knockdown reduces skin tissue hyperplasia and decreases the expression of inflammatory factors in IMQ mice. Mechanistically, ANXA3 knockdown inhibits the NF-κB/STAT3 pathway in skin tissue. Additionally, ANXA3 knockdown inhibits inflammation and hyperproliferation in HaCaT cells. Collectively, these results indicate that ANXA3 alleviates psoriasis progression both <i>in-vivo</i> and <i>in-vitro</i> by inhibiting the NF-κB/STAT3 pathway.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"32-41"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug-induced enterocolitis, a new condition under consideration.","authors":"Lucía Hernando Marín, Ana María Segura Machado, Marta Castro Ramos, Javier Torres-Borrego","doi":"10.15586/aei.v53i2.1314","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1314","url":null,"abstract":"","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"50-51"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Echinocystic acid activates PPARγ to alleviate mannan-induced psoriasis and psoriatic arthritis in mice.","authors":"Chengwei Yu, Huiming Wu, Dongrui Zhao, Huajie Shi","doi":"10.15586/aei.v53i2.1283","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1283","url":null,"abstract":"<p><p>Previous studies have shown that echinocystic acid (EA) can reduce arthritis and skin damage, but the role of EA in psoriatic arthritis is unclear. This study aims to prove the role of EA in psoriatic arthritis, which was induced by intraperitoneal injection of mannan in C57BL/6J mice. The mice were divided into a control group, mannan group, mannan + EA (low-dose) group, and mannan + EA (high-dose) group. Joint tissue damage was scored, and pathological changes in joint tissue and ear skin damage were examined by HE staining. Pathway enrichment of EA drug targets was performed through the target enrichment website, and the mRNA and protein expression levels of pathway-related proteins in joint tissues and ears were verified using the PCR and western blot. The results show that injection of mannan into mice resulted in joint inflammatory infiltration and tissue damage, hyperkeratosis, and acanthosis of the ear skin, while these symptoms were alleviated after high-dose EA treatment. Pathway enrichment analysis showed that the EA drug treatment target is concentrated on the PPAR pathway. The mRNA and protein results showed that the mRNA and protein expression levels of peroxisome proliferator-activated receptor γ (PPARγ) in the joint tissues and ears of mice with psoriatic arthritis decreased, and the expression of PPARγ was activated after high-dose EA treatment. In conclusion, EA increases PPARγ expression and reduces joint and skin damage in mice with psoriatic arthritis.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"52-58"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Idiopathic anaphylaxis due to SARS-CoV-2 mRNA vaccine?","authors":"Öner Özdemir","doi":"10.15586/aei.v53i2.1299","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1299","url":null,"abstract":"","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"8-9"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is hen's egg a significant allergen in adults too? Findings from an investigation into food allergy prevalence.","authors":"Betül Özdel Öztürk, Begüm Görgülü Akın, Makbule Seda Bayrak Durmaz, Şadan Soyyiğit","doi":"10.15586/aei.v53i2.1263","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1263","url":null,"abstract":"<p><strong>Introduction: </strong>Data on adult food allergies (FAs) remain limited compared to the pediatric population, and this study investigates the prevalence and clinical characteristics of FAs among adults.</p><p><strong>Methods: </strong>This retrospective study included adults aged 18-80 diagnosed with FAs at the Immunology and Allergy Diseases Clinic of Ankara Bilkent City Hospital, Turkey, between 2019 and 2024.</p><p><strong>Results: </strong>During the study period, 10,505 patients were examined to diagnose and manage various allergic and immunologic conditions. Among these, 563 patients were suspected of having FAs, which was confirmed in 33 patients (mean age 34.74 ± 12.57 years; 60.6% female), yielding a frequency of 0.31%. The primary allergens identified included hen's egg (n = 6, 18.2%), fish and shellfish (n = 4, 12.1%), and various nuts such as peanuts (n = 2, 6.1%), hazelnut (n = 1, 3.0%), and almond (n = 1, 3.0%) as well as seeds like sesame (n = 2, 6.1%) and sweet corn (n = 2, 6.1%) and fruits such as banana (n = 4, 12.1%) and kiwi (n = 4, 12.1%). The median time interval between food consumption and allergic reactions was 10 minutes (2-120). Anaphylaxis was the most frequently observed clinical presentation (n = 24, 72.7%). Emergency treatments included antihistamines and steroids (n = 15, 65.2%), antihistamines, steroids, and epinephrine (n = 5, 21.7%), or epinephrine alone (n = 3, 13.0%). Egg allergy was the most prevalent allergen (n = 6, 18.2%), characterized by a rapid onset of allergic reactions (median of 5 minutes); two patients reacted regardless of cooking methods and four showed tolerance to thoroughly cooked eggs, with component-resolved diagnostics indicating sensitization to ovalbumin (Gal d2) but not ovomucoid (Gal d1).</p><p><strong>Conclusion: </strong>Our study demonstrates a low frequency of FAs in adults yet highlights the seriousness of reactions like anaphylaxis. Hen's egg allergy was notably prevalent, underscoring the need for increased awareness and improved diagnostic strategies to effectively manage FAs in the adult population.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"13-23"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Delphi consensus on diagnosis, management, and treatment with allergen immunotherapy of polysensitized children in Spain: CAPP study, Part 1.","authors":"María Mesa-Del-Castillo, Francisco J Canals Candela, Ana Martínez-Cañavate, Cristina Rivas-Juesas, Helena Larramona Cabrera, Miguel Tortajada-Girbés, José Manuel Lucas Moreno, Maria Del Mar Folqué, Ana Morales-Tirado, Ana I Tabar","doi":"10.15586/aei.v53i2.1220","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1220","url":null,"abstract":"<p><strong>Background: </strong>The study aimed to evaluate the level of agreement between specialists in pediatric allergology regarding the diagnosis and indications for pollen allergen immunotherapy (AIT) of polysensitized children in Spain.</p><p><strong>Materials and methods: </strong>A Delphi study was performed using an online survey designed by a committee of pediatric AIT experts: 46 and 44 panelists participated in rounds 1 and 2, respectively. In round 1, 204 statements on 8 dimensions were evaluated (Diagnosis; Therapeutic management; Pollens - Part I; Mites; Moulds; Animals; Hymenoptera venom; and Mixtures - Part II). A total of 148 statements were finally accepted after round 2. Panel members rated their level of agreement with assessments on a 9-point Likert scale based on acceptance by ≥ 66.7% of them.</p><p><strong>Results: </strong>According to the results, the polysensitization determination in allergic patients is confirmed by clinical history, skin prick test, total and specific IgE, and molecular diagnostics. Clinical assessments are recommended for the AIT effectiveness evaluation. Follow-ups should be performed 6 months after AIT initiation. According to experts, pollens are the most representative allergens in allergic rhinitis but AIT is more effective in bronchial asthma treatment. The IgE levels are positively related to the intensity of the symptomatology and the efficacy of <i>Grass</i> AIT. In pollen mixtures, a maximum mixture of three AIT allergens is established between <i>Grass, Olive</i>, and <i>Cupressaceae</i>. Mixing pollen from <i>Platanus acerifolia</i> and <i>Parietaria</i> is not recommended.</p><p><strong>Conclusions: </strong>This study provides, where evidence is lacking, current expert-based opinions on clinical decision-making for managing pollen AIT in polysensitized children.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"124-140"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between e-cigarette smoking and different lifestyle patterns among young adults in Saudi Arabia.","authors":"Shatha A Alduraywish, Abdulrahman A Alghamdi, Mohammed A Almutairi, Faisal M Alghthami, Mohand M Alsultan, Laith A Alreshaid, Fahad M Aldakheel, Shabana Tharkar","doi":"10.15586/aei.v53i2.1222","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1222","url":null,"abstract":"<p><strong>Background: </strong>E-cigarette use has been linked with multiple morbidities and changes in health behavior. This study investigates e-cigarette use with sleep patterns, stress levels, and physical activity behavior and compare it with nonsmokers.</p><p><strong>Methods: </strong>A standardized electronic questionnaire for sleep, stress, and physical activity assessment was used on 372 students enrolled in a large university. Statistical analysis was performed using Statistical Package for the Social Sciences (SPSS) version 23.0.</p><p><strong>Results: </strong>The mean age of the study population was around 20 years, with e-cigarette use higher in males. The mean age of smoking initiation was 17 years. The intention to play sports and physical activity differed between smokers and nonsmokers. E-cigarette users practiced sports to remain fit while nonsmokers intended to play sports for enjoyment; however, no significant difference in the level of fitness was found. Sleep behavior and stress levels were similar across the two groups.</p><p><strong>Conclusion: </strong>No difference in lifestyle patterns between e-cigarette users and nonsmokers may be due to lesser smoking duration since initiation. Longitudinal studies with diverse populations and a longer duration of e-cigarette use are recommended to explore the effects of e-cigarette smoking on lifestyle behavior.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"67-73"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-life effectiveness of tezepelumab in severe asthma.","authors":"Juan Carlos Miralles-López, Francisco-José Bravo-Gutierrez, Rubén Andújar-Espinosa, Manuel Castilla-Martínez, Cayetano Díaz-Chantar, Mercedes Ramírez-Hernández, Sheila Cabrejos-Perotti, Maria Jesús Avilés-Inglés, Inmaculada Ibarra-Calabuig, Miguel Henrique Reyes-Cotes, Manuel José Pajarón-Fernández, Roberto Bernabéu-Mora, Consuelo Alcalde-Rumayor, María Loreto Alemany-Francés, José Valverde-Molina, Virginia Pérez-Fernández","doi":"10.15586/aei.v53i2.1326","DOIUrl":"https://doi.org/10.15586/aei.v53i2.1326","url":null,"abstract":"<p><strong>Background: </strong>Tezepelumab is a human monoclonal antibody which inhibits the cytokine thymic stromal lymphopoietin and is effective in different asthma endotypes.</p><p><strong>Methods: </strong>This is a prospective, multicenter study of the Register of Severe Asthma of the Region of Murcia (RE-ASGRAMUR) Group performed under routine clinical practice conditions.</p><p><strong>Results: </strong>We present a series of 57 patients who completed at least 6 months of treatment with tezepelumab. The exacerbations decreased from a baseline mean of 2.63 in the previous year to a <i>mean annual rate</i> of 0.38 after 6 months of tezepelumab (85.6% decrease). The Asthma Control Test score increased by 5.3 points, and the Mini Asthma Quality of Life Questionnaire score increased by 0.94 points. Regarding lung function, we obtained a significant increase of 188 mL in forced expiratory volume in 1 s and 166 mL in forced vital capacity. Finally, type-2 (T2) biomarkers, such as eosinophils and nitric oxide, showed a significant decrease. We compared the results in patients with or without previous biological treatment as well as in patients with or without allergy sensitization, and we did not obtain significant differences in any variable.</p><p><strong>Conclusions: </strong>Tezepelumab is an effective treatment for severe asthma, lessening exacerbations, controlling the disease, and improving quality of life and lung function in patients with and without high T2 biomarkers, regardless of previous biological treatment.</p>","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":"53 2","pages":"163-173"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}