CXCL16 deficiency alleviates ovalbumin-induced allergic rhinitis in mice.

IF 2.1 4区医学 Q3 ALLERGY

Allergologia et immunopathologia Pub Date : 2025-07-01 eCollection Date: 2025-01-01 DOI:10.15586/aei.v53i4.1376

Meihua Kong, Qian He

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引用次数: 0

Abstract

Purpose: Research has indicated that individuals with allergic rhinitis exhibit elevated levels of CXCL16 expression in their serum. This study aims to illustrate the role of CXCL16 and its associated mechanisms in mice suffering from allergic rhinitis.

Methods: An allergic rhinitis model was established by injecting ovalbumin (OVA) into mice, and the expression of CXCL16 mRNA and protein in nasal mucosal tissue was measured. The frequency of nose rubbing and sneezing in each group of mice was recorded. Serum levels of IgE and IgG1 were also assessed. Th2 cell-related factors in the bronchoalveolar lavage fluid (NALF) were analyzed. Histological staining was used to examine pathological changes in the nasal and lung tissues. The expression levels of p-p65, p65, p-IκBα, and IκBα proteins in nasal tissues were evaluated using western blot.

Results: CXCL16 expression was elevated in OVA-induced allergic rhinitis mice. CXCL16 knockout reduced the frequency of nose wiping and sneezing in OVA-induced mice and suppressed the levels of IgE and IgG1. Furthermore, CXCL16 knockout led to a decrease in both the number of inflammatory cells and the levels of inflammatory factors in NALF. Histological staining revealed that CXCL16 knockout alleviated pathological tissue changes and goblet cell hyperplasia. Additionally, CXCL16 knockout suppressed the expression of p-p65/p65 and p-IκBα in nasal tissues, while increasing the expression of IκBα.

Conclusion: CXCL16 deficiency alleviates allergic rhinitis.

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CXCL16缺乏可减轻卵清蛋白诱导的小鼠变应性鼻炎。

目的：研究表明，变应性鼻炎患者血清中CXCL16表达水平升高。本研究旨在阐明CXCL16在小鼠变应性鼻炎中的作用及其相关机制。方法：通过注射卵清蛋白（OVA）建立小鼠变应性鼻炎模型，检测CXCL16 mRNA及蛋白在鼻黏膜组织中的表达。记录各组小鼠摩擦鼻子和打喷嚏的频率。同时检测血清IgE和IgG1水平。分析支气管肺泡灌洗液（nff）中Th2细胞相关因子。采用组织学染色法观察鼻、肺组织的病理变化。western blot法检测p-p65、p65、p- κ b α、i - κ b α蛋白在大鼠鼻组织中的表达水平。结果：ova诱导的变应性鼻炎小鼠中CXCL16表达升高。敲除CXCL16降低了ova诱导小鼠擦鼻和打喷嚏的频率，并抑制了IgE和IgG1的水平。此外，敲除CXCL16导致nff中炎症细胞数量和炎症因子水平下降。组织学染色显示，敲除CXCL16可减轻病理组织改变和杯状细胞增生。此外，敲除CXCL16抑制了p-p65/p65和p- κ b α在鼻组织中的表达，同时增加了i - κ b α的表达。结论：CXCL16缺乏可减轻变应性鼻炎。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.