Sensors & diagnostics最新文献

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Non-organic solvent extraction of capsaicinoids from oil combined with fluorescent lateral flow immunoassay strips for on-site identification of illegally recycled waste cooking oil† 非有机溶剂萃取油中辣椒素结合荧光侧流免疫试纸对非法回收废食用油的现场鉴定
IF 3.5
Sensors & diagnostics Pub Date : 2024-12-20 DOI: 10.1039/D4SD00306C
Yulian Wang, Yuxiang Wu, Deji Gesang, Zixuan Dong, Zhaoxu Qin, Qingchun Li, Jin Li, Qianxu Zhou and Guoqing Shi
{"title":"Non-organic solvent extraction of capsaicinoids from oil combined with fluorescent lateral flow immunoassay strips for on-site identification of illegally recycled waste cooking oil†","authors":"Yulian Wang, Yuxiang Wu, Deji Gesang, Zixuan Dong, Zhaoxu Qin, Qingchun Li, Jin Li, Qianxu Zhou and Guoqing Shi","doi":"10.1039/D4SD00306C","DOIUrl":"https://doi.org/10.1039/D4SD00306C","url":null,"abstract":"<p >The illicit use of recycled waste cooking oil poses a threat to food safety, yet there is currently a lack of on-site identification methods. This study targets a key component of recycled cooking oil, capsaicinoids, and establishes a rapid detection method for identifying illegally recycled waste cooking oil on-site. The method involves extracting capsaicinoids from the oil using a non-organic solvent extractant, and then detecting it using fluorescent lateral flow immunoassay (LFIA) strips. The preparation conditions of LFIA test strips were first optimized in this study. Subsequently, a 0.02 mol L<small><sup>−1</sup></small> solution of dimethyl-β-cyclodextrin was optimized as the extractant for capsaicin. The optimized sample extraction conditions involve a sample-to-extractant volume ratio of 1:2 and an extraction time of 1 minute, with a total extraction and detection time not exceeding 15 minutes. This method demonstrates a limit of detection (LOD) for natural capsaicin in oil samples of 0.14 μg kg<small><sup>−1</sup></small>, with a detection range spanning 0.46 to 81 μg kg<small><sup>−1</sup></small>. The method yields recovery rates between 88.76% and 115.79%, with coefficient of variation (CV) values ranging from 1.80% to 13.37%. Cross-reactivity rates for dihydrocapsaicin and synthetic capsaicin exceed 90%, while the impact of common contaminants or additives in other edible oils on detection is minimal. In conclusion, this approach fulfills the technical criteria mandated by the China Food and Drug Administration for distinguishing capsaicin compounds in recycled oil, offering advantages such as simplicity, rapidity, and “green” operation, making it suitable for rapid on-site identification of illegally recycled waste cooking oil.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 2","pages":" 147-158"},"PeriodicalIF":3.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00306c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The development of GFETs for biometric applications 用于生物识别应用的gfet的发展
IF 3.5
Sensors & diagnostics Pub Date : 2024-12-13 DOI: 10.1039/D4SD00317A
Weisong Yang, Weihao Feng, Siyu Hou, Zhuang Hao, Cong Huang and Yunlu Pan
{"title":"The development of GFETs for biometric applications","authors":"Weisong Yang, Weihao Feng, Siyu Hou, Zhuang Hao, Cong Huang and Yunlu Pan","doi":"10.1039/D4SD00317A","DOIUrl":"https://doi.org/10.1039/D4SD00317A","url":null,"abstract":"<p >Due to the rising prevalence of chronic diseases worldwide attributed to an aging population and the development of big data and artificial intelligence (AI), there is a significant demand for healthcare and long-term disease monitoring. As an emerging sensing technology, graphene field-effect transistor (GFET) sensors are anticipated to become the backbone of future large-scale electronic applications, owing to their rapid and accurate disease diagnosis capabilities, excellent biocompatibility, and ease of system integration. This review summarizes the recent advances in biosensing applications using GFETs. Initially, the working mechanism of GFETs for biosensing is briefly introduced, followed by an outline of various gate configuration strategies employed in GFETs and a discussion on methods for enhancing sensing performance. The multiplexing capabilities and flexible wearable applications of GFETs are then summarized and highlighted, aiming to increase the diversity and applicability of these sensors. Subsequently, a comprehensive survey of the advancements in the integration and miniaturization of multi-component GFET biosensors is discussed. Moreover, this review provides an outlook on the challenges and prospects associated with the commercialization of GFET technology in the biosensing field.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 2","pages":" 111-135"},"PeriodicalIF":3.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00317a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selection of ssDNA aptamers and construction of an aptameric electrochemical biosensor for detecting Giardia intestinalis cyst protein† 检测肠贾第虫囊蛋白†的ssDNA适体筛选及适体电化学生物传感器的构建
IF 3.5
Sensors & diagnostics Pub Date : 2024-12-12 DOI: 10.1039/D4SD00296B
Mohammed Alhindawi, Amina Rhouati, Rahmah Noordin, Dana Cialla-May, Jürgen Popp and Mohammed Zourob
{"title":"Selection of ssDNA aptamers and construction of an aptameric electrochemical biosensor for detecting Giardia intestinalis cyst protein†","authors":"Mohammed Alhindawi, Amina Rhouati, Rahmah Noordin, Dana Cialla-May, Jürgen Popp and Mohammed Zourob","doi":"10.1039/D4SD00296B","DOIUrl":"https://doi.org/10.1039/D4SD00296B","url":null,"abstract":"<p > <em>Giardia intestinalis</em>, an intestinal protozoan parasite, is one of the potentially severe parasitic infections, especially in children. Rapid and simple diagnostic tools are highly desired to prevent the potential outbreak of <em>G. intestinalis</em> infection. The life cycle of Giardia species is quite simple and consists of trophozoite and cystic forms. This report presents the selection of ssDNA aptamers with high binding affinity to a <em>G. intestinalis</em> cyst recombinant protein using the SELEX process (systematic evolution of ligands by exponential enrichment). The process is based on incubating a random DNA library with the targeted protein, and the bound sequences are recovered and amplified by polymerase chain reaction (PCR). The generated pool of aptamer sequences is used in the subsequent selection round. After ten selection cycles, three sequences were isolated with low dissociation constants (<em>K</em><small><sub>d</sub></small>) of 7.98, 21.02, and 21.86 nM. Subsequently, the aptamer with the best affinity was integrated into a label-free electrochemical biosensor to detect <em>G. intestinalis</em> cyst protein. The developed aptasensor accurately detected the <em>G. intestinalis</em> recombinant cyst protein within the range of 0.1 pg mL<small><sup>−1</sup></small> to 1000 ng mL<small><sup>−1</sup></small>, and a low detection limit of 0.0026 pg mL<small><sup>−1</sup></small>. Furthermore, a selectivity study showed insignificant cross-reactivity against other proteins such as bovine serum albumin and globulin, and no reactivity against <em>G. intestinalis</em> trophozoite recombinant protein. Finally, the aptasensor was tested using <em>G. intestinalis</em>-spiked tap water samples and showed good recovery rates.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 82-89"},"PeriodicalIF":3.5,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00296b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Agarose-gel coating for improving the polydopamine-based pH sensor stability in continuous pH measurements† 琼脂糖凝胶涂层用于提高聚多巴胺型pH传感器在连续pH测量中的稳定性
IF 3.5
Sensors & diagnostics Pub Date : 2024-12-11 DOI: 10.1039/D4SD00294F
Natalie Fudge, Fatemeh Keyvani, Joshua Khatri and Mahla Poudineh
{"title":"Agarose-gel coating for improving the polydopamine-based pH sensor stability in continuous pH measurements†","authors":"Natalie Fudge, Fatemeh Keyvani, Joshua Khatri and Mahla Poudineh","doi":"10.1039/D4SD00294F","DOIUrl":"https://doi.org/10.1039/D4SD00294F","url":null,"abstract":"<p >Continuous monitoring in healthcare could transform patient care by improving patient outcomes and reducing costs through rapid detection and timely intervention. The pH of body fluids is an essential indicator for detecting acid/base imbalances and subsequent diseases, as well as monitoring organ functions. However, current pH sensors experience signal drifts over continuous measurements, inhibiting their ability to provide longitudinal body's pH status. To enable real-time, continuous pH monitoring, we developed a polydopamine (PDA)-based pH sensor and validated its performance in PBS and simulated wound fluid exudate (WFE). To increase signal stability for continuous performance, heat treatment and agarose gel coating were applied to the sensor, and we observed that the agarose coating greatly improved the signal drift.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 2","pages":" 136-146"},"PeriodicalIF":3.5,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00294f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of concern: Sensing of COVID-19 spike protein in nasopharyngeal samples using a portable surface plasmon resonance diagnostic system 关注表达:使用便携式表面等离子体共振诊断系统检测鼻咽样本中COVID-19刺突蛋白
IF 3.5
Sensors & diagnostics Pub Date : 2024-12-06 DOI: 10.1039/D4SD90045F
Hiba Saada, Quentin Pagneux, James Wei, Ludovic Live, Alain Roussel, Alexis Dogliani, Lycia Die Morini, Ilka Engelmann, Enagnon Kazali Alidjinou, Anne Sophie Rolland, Emmanuel Faure, Julien Poissy, Julien Labreuche, Gil Lee, Peng Li, Gerard Curran, Anass Jawhari, Jhonny A. Yunda, Sorin Melinte, Axel Legay, Jean-Luc Gala, David Devos, Rabah Boukherroub and Sabine Szunerits
{"title":"Expression of concern: Sensing of COVID-19 spike protein in nasopharyngeal samples using a portable surface plasmon resonance diagnostic system","authors":"Hiba Saada, Quentin Pagneux, James Wei, Ludovic Live, Alain Roussel, Alexis Dogliani, Lycia Die Morini, Ilka Engelmann, Enagnon Kazali Alidjinou, Anne Sophie Rolland, Emmanuel Faure, Julien Poissy, Julien Labreuche, Gil Lee, Peng Li, Gerard Curran, Anass Jawhari, Jhonny A. Yunda, Sorin Melinte, Axel Legay, Jean-Luc Gala, David Devos, Rabah Boukherroub and Sabine Szunerits","doi":"10.1039/D4SD90045F","DOIUrl":"https://doi.org/10.1039/D4SD90045F","url":null,"abstract":"<p >Expression of concern for ‘Sensing of COVID-19 spike protein in nasopharyngeal samples using a portable surface plasmon resonance diagnostic system’ by Hiba Saada <em>et al.</em>, <em>Sens. Diagn.</em>, 2022, <strong>1</strong>, 1021–1031, https://doi.org/10.1039/D2SD00087C.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 102-102"},"PeriodicalIF":3.5,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd90045f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In situ amplified colorimetric immunoassay coupled with a dual-enzyme-functionalized UiO-66(Ce) framework for quantitative detection of the dengue virus† 结合双酶功能化UiO-66(Ce)框架的原位扩增比色免疫分析法用于登革热病毒†的定量检测
IF 3.5
Sensors & diagnostics Pub Date : 2024-11-29 DOI: 10.1039/D4SD00335G
Luanfeng Lin, Xiangqun Fan, Yinmei Yan, Ting Lin, Xiao Han, Dianping Tang and Lifen Han
{"title":"In situ amplified colorimetric immunoassay coupled with a dual-enzyme-functionalized UiO-66(Ce) framework for quantitative detection of the dengue virus†","authors":"Luanfeng Lin, Xiangqun Fan, Yinmei Yan, Ting Lin, Xiao Han, Dianping Tang and Lifen Han","doi":"10.1039/D4SD00335G","DOIUrl":"https://doi.org/10.1039/D4SD00335G","url":null,"abstract":"<p >Signal amplification and noise reduction are very crucial for the sensitive determination of disease-related biomarkers. The development of rapid and laborious testing is urgently required due to extended incubation time and high mortality. Herein, we developed a Ce-based mesoporous metal–organic framework-based multi-enzyme encapsulation device for the realization of enzyme-linked immunosorbent assay (ELISA) for the dengue virus (DENV). Briefly, a UiO-66(Ce) framework structure with uniform mesopores was synthesized by a one-pot method, which allowed efficient encapsulation of natural enzymes with an encapsulation efficiency of 700%. The developed multi-enzyme reaction probes were able to maintain more than 90% of catalytic activity stable at room temperature for 60 days while ensuring efficient enzyme immobilization. Sensitive evaluation of DENV was achieved by encapsulating glucose oxidase and horseradish peroxidase, combined with the formation of an immune sandwich in the presence of DENV. The developed sensor enabled flexible detection of recombinant dengue virus serotype 2 NS1 protein (DENV2-NS1) from 0.05 to 100 ng mL<small><sup>−1</sup></small>, with a low limit of detection of 39.7 pg mL<small><sup>−1</sup></small>. In addition, there were no significant differences in the test results of the samples obtained through the developed multi-enzyme probes when compared to commercially available ELISA kits. This work provides new horizons for the development of efficient enzyme encapsulation systems.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 75-81"},"PeriodicalIF":3.5,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00335g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dengue-virosensor: advancement of dengue virus-based biosensors 登革热病毒传感器:基于登革热病毒的生物传感器的进展
IF 3.5
Sensors & diagnostics Pub Date : 2024-11-22 DOI: 10.1039/D4SD00262H
Mohd. Rahil Hasan, Pradakshina Sharma, Sameer Khan, Ubaid Mushtaq Naikoo, Kapil Bhalla, M. Z. Abdin, Nitesh Malhotra, Tejraj M. Aminabhavi, Nagraj P. Shetti and Jagriti Narang
{"title":"Dengue-virosensor: advancement of dengue virus-based biosensors","authors":"Mohd. Rahil Hasan, Pradakshina Sharma, Sameer Khan, Ubaid Mushtaq Naikoo, Kapil Bhalla, M. Z. Abdin, Nitesh Malhotra, Tejraj M. Aminabhavi, Nagraj P. Shetti and Jagriti Narang","doi":"10.1039/D4SD00262H","DOIUrl":"https://doi.org/10.1039/D4SD00262H","url":null,"abstract":"<p >Dengue is one of the world's fastest-growing health issues, affecting primarily tropical and sub-tropical countries. Dengue infection is spread <em>via</em> mosquitoes, <em>i.e.</em>, <em>Aedes</em>, and caused by the dengue virus (DENV), a single-stranded RNA virus having 4 serotypes, <em>i.e.</em>, DENV 1–4, and any one of the 4-serotypes can cause dengue fever. Dengue symptoms can range from asymptomatic primary infection to fatal secondary infections, such as dengue shock syndrome (DSS) and dengue hemorrhagic fever (DHF). Since there are no dengue vaccinations or antiviral treatments available, total bed rest, proper hydration, and the use of analgesics for symptomatic pain relief are usually prescribed, which make recovery both difficult and time-consuming. This mandates the development of early detection methods for DENV so that the disease can be stopped before it spreads. For example, traditional laboratory-based diagnostic approaches for detecting dengue infection have one or more flaws. This includes cross-reactivity with other flaviviruses, the need for several samples for serological assays, and the high cost and complexity of PCR processes. Thus, biosensors have garnered considerably greater attention because of their simple fabrication, ease of use, ultra-sensitivity, selectivity, and low cost of production. This review starts with an introduction and a discussion on the conventional methods used for dengue virus detection, along with their limitations. Later, recently developed and futuristic biosensors are summarized before and finally closing this review with a brief conclusion.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 7-23"},"PeriodicalIF":3.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00262h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Towards greater accountability and trust: the launch of transparent peer review in Sensors & Diagnostics 向更大的问责制和信任:在传感器和诊断领域推出透明的同行评审
IF 3.5
Sensors & diagnostics Pub Date : 2024-11-20 DOI: 10.1039/D4SD90043J
{"title":"Towards greater accountability and trust: the launch of transparent peer review in Sensors & Diagnostics","authors":"","doi":"10.1039/D4SD90043J","DOIUrl":"https://doi.org/10.1039/D4SD90043J","url":null,"abstract":"<p >A graphical abstract is available for this content</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 12","pages":" 1886-1886"},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd90043j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of NIAD-4 derivatives for fluorescence-based detection of protein aggregates† 用于蛋白聚集体荧光检测的NIAD-4衍生物的研制
IF 3.5
Sensors & diagnostics Pub Date : 2024-11-14 DOI: 10.1039/D4SD00182F
Tze Cin Owyong, Laura E. Shippey, Siyang Ding, David S. Owen, Shouxiang Zhang, Jonathan M. White, Wallace W. H. Wong, David P. Smith and Yuning Hong
{"title":"Development of NIAD-4 derivatives for fluorescence-based detection of protein aggregates†","authors":"Tze Cin Owyong, Laura E. Shippey, Siyang Ding, David S. Owen, Shouxiang Zhang, Jonathan M. White, Wallace W. H. Wong, David P. Smith and Yuning Hong","doi":"10.1039/D4SD00182F","DOIUrl":"https://doi.org/10.1039/D4SD00182F","url":null,"abstract":"<p >Impairment of protein quality control is a critical factor in the development of neurodegenerative disorders like Alzheimer's, Parkinson's and Huntington's disease, characterized by the accumulation of protein aggregates. As such, detection and monitoring of protein aggregates remains a crucial area of study. In this work, we synthesize a series of bithiophene derivatives based on a red emitting amyloid fluorophore NIAD-4. By molecular engineering, widened Stokes shifts and spectral tuning can be achieved in these derivatives. Through molecular docking and aggregation assays, we demonstrate the specificity of these derivatives towards protein fibrils over monomers and amorphous aggregates. Utilizing unbiased flow cytometry together with a cell viability indicator, we show that derivative NIAD-CNOET facilitates the discrimination of cells treated with and without preformed fibrils of α-synuclein, a model of the pathological hallmark of Parkinson's disease.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 55-62"},"PeriodicalIF":3.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00182f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction of DNA template sequences to generate fluorescent gold nanoclusters for the sensitive detection of DNA methyltransferase activity bioassay† 构建DNA模板序列生成荧光金纳米簇,用于DNA甲基转移酶活性生物测定灵敏检测
IF 3.5
Sensors & diagnostics Pub Date : 2024-11-12 DOI: 10.1039/D4SD00215F
Fangyu Zhou, Hui Chen, Tingting Fan, Zixia Guo, Xiangyan Dong and Feng Liu
{"title":"Construction of DNA template sequences to generate fluorescent gold nanoclusters for the sensitive detection of DNA methyltransferase activity bioassay†","authors":"Fangyu Zhou, Hui Chen, Tingting Fan, Zixia Guo, Xiangyan Dong and Feng Liu","doi":"10.1039/D4SD00215F","DOIUrl":"https://doi.org/10.1039/D4SD00215F","url":null,"abstract":"<p >Abnormal DNA methylation mediated by DNA methyltransferases is one of the most common epigenetic modifications, and abnormal DNA methyltransferase activity is often responsible for serious diseases such as cancer. For rapid, sensitive and efficient detection of DNA methyltransferase activity, a bioassay system using gold nanoclusters (DNA-AuNCs) as output has been developed. In this study, dumbbell DNA substrate is recognized and methylated by methyltransferase followed by cleavage by endonuclease (GlaI). In the presence of terminal deoxynucleotidyl transferase (TdT), the poly-A DNA product eventually becomes the template for the reduction of gold nanoclusters and then generated with a strong fluorescence signal. The assay is highly sensitive and requires no amplification or complex material synthesis steps. The detection limit is 0.077 U mL<small><sup>−1</sup></small>. The bioassay showed good detection efficiency in both human serum, cell lysates and human-derived cells. Moreover, it can be used for screening and evaluation of M.SssI MTase inhibitors and hence has great potential use in disease diagnosis and drug discovery. The method was universal and allowed for other biological target detection by simply replacing the sequence of the substrate DNA recognition site; thus the proposed assay has a broad scope of application in both bioassay and drug screening.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 90-101"},"PeriodicalIF":3.5,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00215f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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