Sensors & diagnostics最新文献

{"title":"Correction: Rapid detection of Candida albicans in urine by an Electrochemical Impedance Spectroscopy (EIS)-based biosensor","authors":"Tina D'Aponte, Maria De Luca, Nikola Sakač, Martina Schibeci, Angela Arciello, Emanuela Roscetto, Maria Rosaria Catania, Vincenzo Iannotti, Raffaele Velotta and Bartolomeo Della Ventura","doi":"10.1039/D5SD90051D","DOIUrl":"https://doi.org/10.1039/D5SD90051D","url":null,"abstract":"<p >Correction for ‘Rapid detection of <em>Candida albicans</em> in urine by an Electrochemical Impedance Spectroscopy (EIS)-based biosensor’ by Tina D'Aponte <em>et al.</em>, <em>Sens. Diagn.</em>, 2023, <strong>2</strong>, 1597–1604, https://doi.org/10.1039/d3sd00209h.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 4","pages":" 611-611"},"PeriodicalIF":4.1,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/sd/d5sd90051d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biophysical characterization of <i>sp</i>Cas9 binding and cleavage using real-time electronic biosensors.","authors":"Deependra Kumar Ban, Kshama Parate, Deepta Bharadwaj, Austin Wong, Lorelai Schoch, Kenneth Visk, Kiana Aran","doi":"10.1039/d5sd00227c","DOIUrl":"https://doi.org/10.1039/d5sd00227c","url":null,"abstract":"<p><p>CRISPR-Cas9 enables curative genome editing but requires precise control of target recognition, particularly when single-nucleotide polymorphisms (SNPs) influence specificity. Conventional biochemical and optical assays often rely on endpoint or ensemble-averaged measurements and therefore fail to resolve the real-time binding dynamics underlying off-target interactions. Here, we report a label-free, non-faradaic electrochemical impedance spectroscopy (nfEIS) platform that directly monitors <i>sp</i>Cas9-gRNA interactions on gold microelectrodes with single-base resolution at the sickle cell disease (SCD) locus. A guide RNA was designed to perfectly match the SCD mutation (A to T) while introducing a single PAM-proximal mismatch with the wild-type DNA (WD) sequence. Using 63-nucleotide synthetic DNA substrates representing SCD and WD targets, concentration-dependent binding assays were performed to extract equilibrium parameters. Hill-model analysis revealed higher affinity for the SCD target (<i>k</i> _D = 0.09 nM) relative to WD (<i>k</i> _D = 0.3 nM), confirming strong on-target binding and weakened interaction at the mismatch site. Magnesium dependence evaluation showed that 5 mM Mg²⁺ enhanced discrimination by stabilizing on-target complexes while destabilizing mismatched binding, whereas at 1 mM Mg²⁺ this selectivity was lost. Time-resolved kinetic measurements using 1 nM <i>sp</i>Cas9 and exponential fitting of the curve revealed rapid association (<i>t</i> _1/2 = 1.85 min) and dissociation rates (<i>t</i> _1/2 = 5.24 min) for SCD, consistent with efficient R-loop formation. In contrast, the WD target exhibited slower association (<i>t</i> _1/2 = 2.68 min) and recurring transient binding with delayed dissociation (<i>t</i> _1/2 = 34.38 min), corroborated by endpoint gel assays. Cas9 lacking gRNA showed only weak, unstable interactions. Overall, these results demonstrate that Cas9 specificity arises from both affinity differences and binding-residence dynamics. nfEIS thus provides a real-time, label-free platform for probing Cas9 fidelity, Mg²⁺-dependent activation, and gRNA design for therapeutic genome editing and diagnostics.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147488987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to “Advances in Nanomaterials for Sensors in Early Disease Diagnosis”","authors":"Anitha Devadoss, Suman Singh and Murugan Veerapandian","doi":"10.1039/D6SD90004F","DOIUrl":"https://doi.org/10.1039/D6SD90004F","url":null,"abstract":"<p >A graphical abstract is available for this content</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 4","pages":" 438-440"},"PeriodicalIF":4.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/sd/d6sd90004f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A DSE–ESIPT-active organic luminogen for “turn-on” enantioselective recognition of chiral amino alcohols and selective hydrazine sensing","authors":"Aakash Venkatesan, Baru Venkata Naga Sahithi, Francis Joy, Rupali Patel, Aatika Nizam and Vasantha Veerappa Lakshmaiah","doi":"10.1039/D5SD00214A","DOIUrl":"https://doi.org/10.1039/D5SD00214A","url":null,"abstract":"<p >The development of dual-state emissive (DSE) organic luminogens has elevated the ease of recognition of various biological analytes, which demonstrates the multifaceted potential of dual-state emitters. Therefore, in this study, we synthesised a dual-state emissive excited-state intramolecular proton transfer (ESIPT)-based organic luminogen, (<em>E</em>)-4-(5-bromo-2-hydroxybenzylideneamino)-2,3-dimethyl-1-phenyl-1,2-dihydropyrazol-5-one (ANMB), exhibiting excitation-dependent phototunability with large Stokes shifts of 109 nm and 155 nm in both the solution and solid states, respectively, underscoring its potential as a biosensor. The metal-chelating ability of ANMB was investigated, revealing significant fluorescence quenching upon coordination with Cu<small>²⁺</small> ions, leading to 96% reduction in emission intensity. The introduction of biological analytes, such as amino alcohols, enabled fluorescence recovery, where ANMB demonstrated enantioselective recognition: a single emission peak for the <em>S</em>-enantiomer and dual emission peaks for the <em>R</em>-enantiomer. Furthermore, ANMB demonstrated high selectivity for hydrazine detection in both the solution and solid states, with new emission bands observed at 411 nm and 432 nm, respectively, indicating a fluorescence shift from green to blue. Complementarily, ANMB was successfully applied for real-time imaging of hydrazine in food and plant samples, showcasing its practical adaptability. Additionally, <em>in silico</em> molecular docking studies were performed, revealing the potential therapeutic activity of ANMB against diarrheal targets. Overall, this work highlights the multifunctionality and tunability of DSE–ESIPT-based organic luminogens, positioning ANMB as a promising candidate for the selective recognition of biologically significant analytes in analytical and real-world contexts.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 4","pages":" 579-595"},"PeriodicalIF":4.1,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/sd/d5sd00214a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Rapid detection of major Gram-positive pathogens in ocular specimens using a novel fluorescent vancomycin-based probe","authors":"Richa Sharma, Harinee Rajagopalan, Maxime Klausen, Mani Vimalin Jeyalatha, Muhammed Üçüncü, Seshasailam Venkateswaran, Appakkudal R. Anand and Mark Bradley","doi":"10.1039/D5SD90048D","DOIUrl":"https://doi.org/10.1039/D5SD90048D","url":null,"abstract":"<p >Correction for ‘Rapid detection of major Gram-positive pathogens in ocular specimens using a novel fluorescent vancomycin-based probe’ by Richa Sharma <em>et al.</em>, <em>Sens. Diagn.</em>, 2022, <strong>1</strong>, 1014–1020, https://doi.org/10.1039/d2sd00061j.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 3","pages":" 425-425"},"PeriodicalIF":4.1,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/sd/d5sd90048d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147558613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Noninvasive and point-of-care screening of snoring by breath monitoring using ion-in-conjugation polymer-based humidity sensors","authors":"Ze-Kun Chen, Wei-Wei Bai, Ying-Qian Huo and Jing-Hui He","doi":"10.1039/D5SD90050F","DOIUrl":"https://doi.org/10.1039/D5SD90050F","url":null,"abstract":"<p >Correction for ‘Noninvasive and point-of-care screening of snoring by breath monitoring using ion-in-conjugation polymer-based humidity sensors’ by Ze-Kun Chen <em>et al.</em>, <em>Sens. Diagn.</em>, 2023, <strong>2</strong>, 721–725, https://doi.org/10.1039/D3SD00042G.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 3","pages":" 427-427"},"PeriodicalIF":4.1,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/sd/d5sd90050f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147558615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Diffuse reflectance-based spectroscopic technique for real-time estimation of localized blood oxygenation parameters from human fingertips: a preliminary study","authors":"Ajay Kumar, Kalaivani Chellappan, Aulia Nasution, Dnyandeo Pawar, Manoj Kumar Patel and Rajesh Kanawade","doi":"10.1039/D5SD90049B","DOIUrl":"https://doi.org/10.1039/D5SD90049B","url":null,"abstract":"<p >Correction for ‘Diffuse reflectance-based spectroscopic technique for real-time estimation of localized blood oxygenation parameters from human fingertips: a preliminary study’ by Ajay Kumar <em>et al.</em>, <em>Sens. Diagn.</em>, 2022, <strong>1</strong>, 1236–1242, https://doi.org/10.1039/D2SD00126H.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 3","pages":" 426-426"},"PeriodicalIF":4.1,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/sd/d5sd90049b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147558614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-cost printed circuit board (PCB) electrochemical biosensors for rapid and label-free detection of Streptococcus pneumoniae","authors":"Vincent Vezza, Veerappan Mani, Niamh Docherty, Adrian Butterworth, David Alcorn, Paul A. Hoskisson and Damion Corrigan","doi":"10.1039/D5SD00210A","DOIUrl":"https://doi.org/10.1039/D5SD00210A","url":null,"abstract":"<p >Severe sepsis presents a critical healthcare challenge where rapid pathogen identification is vital for timely intervention. Current diagnostic methods, however, remain inadequate, often delaying targeted treatment. Using readily available printed circuit board (PCB) electrodes, we address this need by developing a low-cost electrochemical DNA biosensor for rapid detection of <em>Streptococcus pneumoniae</em> using the <em>lytA</em> gene as a biomarker. Through systematic evaluation of commercial and custom PCB designs (P1–P4), gold-plated PCB P4 was found as the optimal platform, demonstrating sensitive detection of <em>lytA</em> sequences (20 bp at 4.50 pM limit of detection in buffer) and clinically relevant 235 bp polymerase chain reaction (PCR) amplicons in 100% human serum (1.0–100 pM) within 15 min at room temperature using electrochemical impedance spectroscopy. The performance of the biosensor originates from the optimized electrode geometry, surface properties, and robust self-assembled monolayer functionalization, enabling specific recognition of bacterial DNA without sample pretreatment. This work establishes PCB-based biosensors as a promising solution for point-of-care sepsis diagnostics, offering significant advantages in speed, cost, and operational simplicity compared to conventional methods.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 4","pages":" 596-610"},"PeriodicalIF":4.1,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/sd/d5sd00210a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular sensing transforms olfactory diagnosis in traditional Chinese medicine: a critical review","authors":"Ling Leng, Ruihan Zhang, Yuxia Shan, Rong Chen, Minghui Yang, Zhenze Cui and Yuan Liang","doi":"10.1039/D5SD00196J","DOIUrl":"https://doi.org/10.1039/D5SD00196J","url":null,"abstract":"<p >Objectifying and standardizing diagnostic methods are essential steps toward the modernization and global recognition of traditional Chinese medicine (TCM). The “four diagnostic methods”, namely, inspection, auscultation and olfaction, inquiry, and palpation, constitute the fundamental diagnostic framework of TCM, among which olfactory diagnosis plays a vital role. This method relies on identifying characteristic odors from the breath or secretions of a patient to guide syndrome differentiation (Bian Zheng). However, conventional olfactory diagnosis remains highly subjective depending on the practitioner's experience, which results in inconsistent outcomes and challenges in reproducibility and quantification. The integration of medical science with modern sensing and analytical technologies provides a transformative pathway to overcome these limitations. Recent studies have shown that exhaled breath contains a complex spectrum of volatile organic compounds (VOCs) that collectively form a personalized “breathprint”, reflecting physiological and pathological states. Objective analysis of these VOCs enables quantifiable, evidence-based characterization of disease-related odors, thereby providing a scientific foundation for olfactory diagnostics in TCM. This review summarizes advancements in VOC detection methodologies, including gas chromatography-mass spectrometry (GC-MS) and electronic nose (e-nose) systems paired with data-driven analytical frameworks, to advance the transformation of traditional olfactory diagnosis in TCM into a standardized, evidence-based diagnostic paradigm.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 4","pages":" 441-454"},"PeriodicalIF":4.1,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/sd/d5sd00196j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoplasmonic core–shell nanoraspberry chip for ultrasensitive surface-enhanced Raman scattering detection of SARS-CoV-2: a modular nanobiosensor for respiratory virus diagnostics","authors":"Nnamdi Nwahara, Saba Niaz, Muthumuni Managa, Christian I. Nkanga, Oluwasesan Adegoke and Ojodomo J. Achadu","doi":"10.1039/D5SD00185D","DOIUrl":"https://doi.org/10.1039/D5SD00185D","url":null,"abstract":"<p >The development of robust and ultrasensitive point-of-care diagnostics for viral pathogens, particularly for seasonal respiratory viruses, remains a critical challenge, as traditional biosensing platforms often suffer from signal instability and poor performance in complex biological matrices. Here, we report a dual-component surface-enhanced Raman scattering (SERS) immunosensor designed to mitigate some of these limitations through a new hotspot engineering strategy. Our platform is centred on a core–shell Au@Ag nanotag fabricated from branched gold nanoraspberries (AuNRBs), which provides a high density of plasmonic hotspots. A conformal Ag shell confines the 4-aminothiophenol (4-ATP) reporter within these nanogaps, establishing a unique “gap-confined” Raman reporter architecture that enhances signal stability and reproducibility. This nanotag is complemented by a second SERS-active component: a capture sensor chip of fluorine-doped tin oxide (FTO) modified with a dense layer of Au nanoparticles (AuNPs). When implemented in a sandwich immunoassay for the SARS-CoV-2 S2 spike protein, this dual-enhancement platform achieved a sensitive detection range of 1–500 ng mL<small>⁻¹</small> with an ultralow limit of detection of 1.07 pg mL<small>⁻¹</small> (15.3 fM) in PBS and 1.30 pg mL<small>⁻¹</small> (18.6 fM) in human serum albumin (HSA). Although these results demonstrate strong analytical sensitivity and rapid assay performance within 30 min, further studies are required to assess clinical robustness, scalability and long-term stability. Overall, this work demonstrates the potential of the proposed SERS system as a versatile platform for rapid, on-site pathogen diagnostics.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 4","pages":" 567-578"},"PeriodicalIF":4.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/sd/d5sd00185d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}