Sensors & diagnostics最新文献

{"title":"Development of a selective-iodide indicator for live-cell imaging and evaluation of CFTR activity.","authors":"Jared Morse, Prasanna Ganesh, Kathrine Cowart, Gabriella Ballestas, Fung Kit Tang, Kaho Leung","doi":"10.1039/d5sd00086f","DOIUrl":"10.1039/d5sd00086f","url":null,"abstract":"<p><p>Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I^- transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(iii) complex (<b>I-Sense</b>) for the selective and pH-independent imaging of intracellular I^-. By tracking cellular iodide I^- uptake, <b>I-Sense</b> facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144981892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to “Lateral Flow Assays: Methods and Applications”","authors":"Jing Wang, Jiangjiang Zhang and Yanmin Ju","doi":"10.1039/D5SD90027A","DOIUrl":"https://doi.org/10.1039/D5SD90027A","url":null,"abstract":"<p >A graphical abstract is available for this content</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 721-722"},"PeriodicalIF":4.1,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd90027a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive electrochemical detection of methotrexate in biological fluids using NiMn2O4/CNT nanocomposite-modified electrode†","authors":"Nasir Abbas and Tae Hyun Kim","doi":"10.1039/D5SD00064E","DOIUrl":"https://doi.org/10.1039/D5SD00064E","url":null,"abstract":"<p >Methotrexate (MTX) is a widely used chemotherapeutic drug with a narrow therapeutic index, making its precise monitoring crucial for effective treatment and minimizing side effects. This study focuses on the development of a clinically applicable NiMn<small>₂</small>O<small>₄</small>/CNT nanocomposite-modified glassy carbon electrode (NiMn<small>₂</small>O<small>₄</small>/CNT-GCE) for the sensitive and selective electrochemical detection of MTX. The NiMn<small>₂</small>O<small>₄</small> nanomaterial was synthesized <em>via</em> a co-precipitation method followed by calcination, and its composite with CNTs was optimized to enhance electrochemical performance. The sensor demonstrated a detection limit as low as 0.627 nM and a broad linear detection range (0.05–3 μM), attributed to the synergistic effects of NiMn<small>₂</small>O<small>₄</small> and CNTs that enhance electron transfer and active site availability. Moreover, the NiMn<small>₂</small>O<small>₄</small>/CNT-GCE was successfully applied to detect MTX in spiked serum and urine samples, achieving recovery rates of 96–99% with relative standard deviations below 3.5%. Its minimal interference with common metabolites and excellent stability makes it ideal for therapeutic drug monitoring. This work underscores the potential of NiMn<small>₂</small>O<small>₄</small>/CNT as a promising platform for real-time clinical diagnostics and advanced electrochemical sensing applications.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 803-814"},"PeriodicalIF":4.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00064e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene nanoplatelet–nickel ferrite coated textile-based embroidered capacitive pressure sensor for wearable electronics application†","authors":"Aqrab ul Ahmad, Saima Qureshi, Mitar Simić, Hafiz Abdul Mannan, Sonam Goyal, Francis Leonard Deepak and Goran M. Stojanović","doi":"10.1039/D5SD00046G","DOIUrl":"https://doi.org/10.1039/D5SD00046G","url":null,"abstract":"<p >In recent times, pressure sensors developed from e-textiles have gained tremendous attention due to their flexibility, comfort, real-time detection, and potential for long-term applications when integrated with monitoring devices. The current research focuses on designing a capacitive pressure sensor comprising a porous textile substrate for electrodes and a porous textile-based dielectric layer. A solution processing approach was used to formulate a graphene nanoplatelet/nickel ferrite (GNP–NiFe<small>₂</small>O<small>₄</small>) composite, and the dip-coating technique was utilized to coat the sensing layer on pure cotton and cotton–polyester fabric. The coated fabric was integrated as a dielectric layer above the interdigitated capacitor to observe the capacitance variation under applied pressure. Additionally, the effects of the volume percentage of GNPs in GNP–NiFe<small>₂</small>O<small>₄</small> and the fabric type on the sensor performance were also considered. The highest sensitivity was obtained for the cotton/polyester textile coated with 10 wt% GNP–NiFe<small>₂</small>O<small>₄</small>. The proposed pressure sensor can reach the linear band in the range from 11 kPa to 100 kPa, making it suitable for pressure sensing in cases of physical impact. Furthermore, a large-area, wireless array of six pressure sensors has been fabricated from the optimized dielectric textile coated with GNP–NiFe<small>₂</small>O<small>₄</small>. The change in the pressure range due to multiple sensors can be monitored on a smartphone, enabling real-time applications in monitoring human body motion, human tactile sensing, or any external pressure in cases of gait or grip.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 779-790"},"PeriodicalIF":4.1,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00046g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel approach to monitor local tissue ischemia associated with pressure ulcers using an optical fibre carbon dioxide sensor†","authors":"Nadia Afroze, Serhiy Korposh, Ricardo Correia, Peter R. Worsley, Barrie R. Hayes-Gill, Seung-Woo Lee and Stephen P. Morgan","doi":"10.1039/D5SD00043B","DOIUrl":"https://doi.org/10.1039/D5SD00043B","url":null,"abstract":"<p >In this paper, we describe the development of a novel approach to monitor local tissue ischemia associated with pressure ulcer using an optical fibre carbon dioxide sensor. Carbon dioxide (CO<small>₂</small>) is a potential biomarker for local tissue ischemia associated with pressure ulcer (PU) formation. Skin CO<small>₂</small> measurement during loading could provide an earlier indicator for pressure induced tissue damage. This study presents a reflection mode optical fibre CO<small>₂</small> sensor (OFCS) that was fabricated and evaluated for measuring skin CO<small>₂</small> during mechanical loading. The optical fibre tip was coated with organically modified silica gel (ormosil) film (thickness 7.23 ± 0.52 μm) containing thymol blue using a dip coating process. Thymol blue has an absorption peak at a wavelength of ~600 nm with an amplitude proportional to CO<small>₂</small> concentration. The OFCS had a typical response time of approximately 60 seconds and a recovery time of 400 seconds for a 0–5.5% CO<small>₂</small> range. OFCSs were tested on the human skin of six healthy volunteers with corresponding CO<small>₂</small> peak values ranging from 145 ppm to 429 ppm with a percent error range of 6–32.2%. The increase in CO<small>₂</small> emitted from the skin during loading offers future promise for alerting the early stage of PU formation.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 791-802"},"PeriodicalIF":4.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00043b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CO2-sensitive inks for the rapid measurement of total viable count (TVC) using micro-respirometry†","authors":"Sean Cross, Christopher O'Rourke and Andrew Mills","doi":"10.1039/D5SD00078E","DOIUrl":"https://doi.org/10.1039/D5SD00078E","url":null,"abstract":"<p >At present, micro-respirometry for measuring total viable count, O<small>₂</small> μR-TVC, is based on the time taken, TT, for an inoculum to significantly reduce the dissolved O<small>₂</small> level (typically from 21% to ≤ 10.5%). Here, a simple kinetic model relevant to μR-TVC is presented which describes the growth of the bacteria from an initial inoculum, <em>N</em><small>_o</small>, to a maximum level, <em>N</em><small>_max</small>, and concomitant consumption of O<small>₂</small> and generation of CO<small>₂</small>, in which the half-way time point, <img>, corresponds to <em>N</em><small>_max</small>/<em>N</em><small>_o</small> = 0.5, at which point %O<small>₂</small> = %CO<small>₂</small> = 10.5%. The model shows that it is not possible to reduce the TT in O<small>₂</small> μR-TVC below <img>, as TT increases above <img> with increasing sensitivity of the O<small>₂</small> sensor. In contrast, the same model shows that if a CO<small>₂</small> sensor is used instead, TT can be reduced significantly below <img> and consequently CO<small>₂</small> μR-TVC could be made much faster than conventional O<small>₂</small> μR-TVC. To test this model prediction, a range of colourimetric CO<small>₂</small> sensors of varying sensitivity, <em>α</em>, were prepared and used to make CO<small>₂</small> μR-TVC measurements. The results confirm that the greater the sensitivity of the sensor, the shorter the TT, as predicted by the kinetic model. Two CO<small>₂</small> indicators, one of moderate sensitivity and one of high sensitivity were used to generate straight-line log(CFU mL<small>⁻¹</small>) <em>vs.</em> TT calibration plots, which can then be used to determine the unknown TVCs of subsequent samples. The future of CO<small>₂</small> μR-TVC as a possible new, faster alternative to conventional O<small>₂</small> μR-TVC is discussed briefly.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 767-778"},"PeriodicalIF":4.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00078e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Array-based polymer-phage biosensors for detection and differentiation of bacteria†","authors":"Enkhlin Ochirbat, Junwhee Yang, Aritra Nath Chattopadhyay, Jungmi Park, Mingdi Jiang, Jan Paczesny and Vincent M. Rotello","doi":"10.1039/D5SD00069F","DOIUrl":"10.1039/D5SD00069F","url":null,"abstract":"<p >Pathogenic bacteria, such as methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), pose significant challenges to public health due to their resistance to conventional antibiotics. Early and accurate identification of bacterial species and discrimination of their strains is critical for guiding effective treatments and infection control. In this study, we develop a polymer-phage sensor platform that integrates polymer-based fluorescence sensing with phage-host specificity for bacterial identification. The sensor successfully differentiates three bacterial species (<em>S. aureus</em>, <em>E. coli</em>, and <em>B. subtilis</em>) and closely related strains of <em>S. aureus</em> (methicillin-sensitive <em>Staphylococcus aureus</em> (MSSA) and MRSA) with high classification accuracy (94–100%) and correct unknown identification rates (94–100%) under optimized conditions. By leveraging phage-host interactions and polymer binding properties, the polymer-phage sensor overcomes the limitations of traditional “lock-and-key” biosensors, offering enhanced specificity and reliability. This platform's rapid response time and adaptability make it a promising tool for clinical diagnostics and public health applications, particularly in combating antibiotic-resistant bacteria.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 759-766"},"PeriodicalIF":4.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12235245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Holographic hydrogel bandage sensor for continual monitoring of wound healing†","authors":"Yihan Zhang, Yubing Hu, Zhenkang Zhu, Yunuen Montelongo, Yanting Liu, Shihabuddeen Waqar, Yoon Soo Park, Leon CZ Chan, Nan Jiang and Ali K. Yetisen","doi":"10.1039/D5SD00047E","DOIUrl":"https://doi.org/10.1039/D5SD00047E","url":null,"abstract":"<p >Chronic wounds pose serious health and economic challenges. A low calcium (Ca<small>²⁺</small>) ion concentration during the early stage often indicates infections. Holographic hydrogel sensors offer label-free sensing platforms, providing real-time and continuous detections of analytes upon diffractive wavelength changes detectable by the naked eye or spectrophotometers, improving the Ca<small>²⁺</small> ion concentration quantification accessibility. Herein, we present a holographic Ca<small>²⁺</small> ion bandage sensor using carboxylate-containing hydrogels on polydimethylsiloxane (PDMS) substrates for real-time wound-healing assessment through smartphone readout. Simulations are conducted to investigate the effects of mechanical strength on sensitivity. The holographic Ca<small>²⁺</small> ion sensor replays blueshifts of 35 nm (hue value change of 7) with 0–4 mmol L<small>⁻¹</small> Ca<small>²⁺</small> ions, changing colors from dark red to red within 7 minutes. It can accurately and stably (over 24 hours) measure Ca<small>²⁺</small> ions when bent. The stiffness of PDMS was tuned to balance comfort and sensitivity. In point-of-care settings, holographic bandage sensors, comprising the holographic hydrogel sensor, a backing layer, and a dark cotton layer, can continuously monitor Ca<small>²⁺</small> ions over 10 hours <em>via</em> a smartphone application using hue values. A guiding square in the application assists users in capturing pictures within the inherently narrow viewing angle range of 20–33°. This holographic Ca<small>²⁺</small> ion bandage sensor facilitates personalized wound assessment through colorimetric interrogation <em>via</em> smartphone readout.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 736-749"},"PeriodicalIF":4.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00047e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot assembling pyrroloquinoline quinone glucose dehydrogenase with polydopamine to overcome the reproducibility issues of layer-by-layer electrode development†","authors":"Alessandra Cimino, Shixin Wang, Verdiana Marchianò, Angelo Tricase, Angela Stefanachi, Eleonora Macchia, Blanca Cassano, Luisa Torsi, Xiaoming Zhang and Paolo Bollella","doi":"10.1039/D5SD00053J","DOIUrl":"10.1039/D5SD00053J","url":null,"abstract":"<p >The reproducibility of enzyme-based biosensors remains a critical challenge, particularly in clinical and wearable applications. Here, we present a novel one-pot polydopamine (PDA)-assisted immobilization strategy for pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) on graphite electrodes to address the limitations of conventional layer-by-layer (LbL) methods. The (PQQ-GDH/PDA)<small>_OPA</small>/G platform demonstrated a uniform and nanostructured enzyme–polymer matrix, confirmed by SEM and spectroscopic characterization, resulting in enhanced surface coverage and enzyme stabilization. Electrochemical analyses revealed an onset potential of +0.19 ± 0.01 V and a maximum current of 0.87 ± 0.08 μA in the presence of glucose. Amperometric calibration yielded a linear range of 0.4–1.2 mM, a sensitivity of 0.47 μA mM<small>⁻¹</small>, and a low detection limit of 26 ± 2 μM. Michaelis–Menten kinetic analysis provided an <em>I</em><small>_max</small> of 1.13 ± 0.07 μA and a <em>K</em><small>^app</small><small>_M</small> of 3.11 ± 0.59 mM. Reproducibility was excellent, with relative standard deviations below 8% for all key parameters. The biosensor retained full functionality under physiological conditions (pH 7.2, 37 °C) and exhibited high selectivity against common interferents, including dopamine, uric acid, and ascorbic acid, with signal variations below 5%. Remarkably, the sensor maintained stable responses in artificial serum for over 67 days, confirming its long-term operational stability. These findings highlight the one-pot PDA-based approach as a scalable, reproducible, and biocompatible platform for next-generation glucose biosensors suitable for real-world biomedical monitoring.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 750-758"},"PeriodicalIF":4.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144593153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms","authors":"Gonzalo E. Fenoy, Waldemar A. Marmisollé, Wolfgang Knoll and Omar Azzaroni","doi":"10.1039/D5SD90021B","DOIUrl":"https://doi.org/10.1039/D5SD90021B","url":null,"abstract":"<p >Correction for ‘Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms’ by Gonzalo E. Fenoy <em>et al.</em>, <em>Sens. Diagn.</em>, 2022, <strong>1</strong>, 139–148, https://doi.org/10.1039/D1SD00007A.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 7","pages":" 632-632"},"PeriodicalIF":3.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd90021b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}