Sensors & diagnostics最新文献_第2页

Fc@ZeNose platform for the detection of four physiologically relevant breath biomarkers: a case study using ethanol, isopropanol, acetic acid, and acetone† Fc@ZeNose检测四种生理相关呼吸生物标志物的平台：使用乙醇，异丙醇，醋酸和丙酮†的案例研究

IF 4.1

Sensors & diagnostics Pub Date : 2025-06-18 DOI: 10.1039/D5SD00038F

Nikini Subawickrama Mallika Widanaarachchige, Anirban Paul, Sriram Muthukumar and Shalini Prasad

{"title":"Fc@ZeNose platform for the detection of four physiologically relevant breath biomarkers: a case study using ethanol, isopropanol, acetic acid, and acetone†","authors":"Nikini Subawickrama Mallika Widanaarachchige, Anirban Paul, Sriram Muthukumar and Shalini Prasad","doi":"10.1039/D5SD00038F","DOIUrl":"https://doi.org/10.1039/D5SD00038F","url":null,"abstract":"Metabolomics allows the analysis of metabolites in biological samples to identify biomarkers associated with metabolic processes, and among these volatile organic compounds (VOCs) have emerged as a significant component in non-invasive diagnostics playing a crucial role in understanding physiological and pathological conditions. The changes in metabolic pathways that occur in biological systems during disease states result in the generation of VOCs as end products or intermediate products. These are then transported to the lungs via the circulatory system and presented into breath at the alveolar membrane. This direct link between metabolic changes and exhaled VOCs has driven growing interest in breathomics, a non-invasive approach to disease diagnosis and monitoring. Among numerous gas sensing technologies that have been explored, electrochemical sensors have demonstrated high sensitivity, cost-effectiveness, real-time monitoring, and miniaturization capabilities. In this work, we have developed a ferrocene (Fc) encapsulated zeolitic imidazole framework −8 (ZIF-8) for the detection of 4 physiologically relevant VOCs: ethanol, isopropanol, acetic acid, acetone, utilizing chronoamperometry as the transduction principle. The material characterization was performed using X-ray photoelectron spectroscopy, powder X-ray diffraction, field emission scanning electron microscopy, energy-dispersive X-ray analysis, and thermogravimetric analysis to confirm the morphological properties of Fc@ZIF-8. The dose-dependent response curves were established for each VOC, demonstrating linearity and the sensor's detection capabilities. Additionally, the sensor's accuracy was confirmed with spike and recovery experiments, achieving recovery rates within the CLSI guideline range of 80–120%.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 723-735"},"PeriodicalIF":4.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00038f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: 3D-printed electrochemical cells for multi-point aptamer-based drug measurements 校正：用于多点适配体药物测量的3d打印电化学电池。

IF 3.5

Sensors & diagnostics Pub Date : 2025-05-29 DOI: 10.1039/D5SD90016F

John Mack, Raygan Murray, Kenedi Lynch and Netzahualcóyotl Arroyo-Currás

引用次数: 0

Innovative and sensitive detection of a cancer cell line using a GMR sensor-based biochip prototype for diagnosis purposes† 使用基于GMR传感器的生物芯片原型用于诊断目的的癌细胞系的创新和敏感检测

IF 3.5

Sensors & diagnostics Pub Date : 2025-05-22 DOI: 10.1039/D5SD00029G

A. Trillat, M. Deroo, M. Giraud, E. Fabre Paul, A. Solignac, P. Bonville, F. Coneggo, A. Afroun, M. Thévenin, A. Wijkhuisen, C. Fermon, S. Simon, A. Duret, G. Cannies, V. Padilla, F. Doucet-Populaire, G. Jasmin-Lebras and C. Féraudet-Tarisse

{"title":"Innovative and sensitive detection of a cancer cell line using a GMR sensor-based biochip prototype for diagnosis purposes†","authors":"A. Trillat, M. Deroo, M. Giraud, E. Fabre Paul, A. Solignac, P. Bonville, F. Coneggo, A. Afroun, M. Thévenin, A. Wijkhuisen, C. Fermon, S. Simon, A. Duret, G. Cannies, V. Padilla, F. Doucet-Populaire, G. Jasmin-Lebras and C. Féraudet-Tarisse","doi":"10.1039/D5SD00029G","DOIUrl":"https://doi.org/10.1039/D5SD00029G","url":null,"abstract":"For several years now, the development of rapid, sensitive, portable and inexpensive early diagnosis techniques has been the focus of increasing attention in the healthcare field, for both primary care and emergency medicine. We have previously demonstrated the proof-of-concept of a patented microfluidic biochip integrating a giant magnetoresistance (GMR)-based sensor, placed on either side of the channel, allowing for the one-by-one dynamic detection of single magnetically labeled biological targets, in a continuous flow mode. In this article, we implemented this two-stage GMR sensor to improve the readiness level of this technology and move towards point-of-care (POC) analysis. We used semi-complex culture medium samples spiked with a murine cancer cell line, pre-labeled with functionalized magnetic particles, to evaluate the biochip performances in detail. The quantitative detection of target cells in low concentrated samples was achieved, with a sensitivity of 5 × 102 cells per mL at a 2 mL per hour flow rate and good specificity, even after addition of irrelevant cells to the sample. Finally, we demonstrated that these performances are competitive with existing techniques such as ELISA tests and flow cytometry analysis, paving the way for new GMR-based POC tests.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 7","pages":" 596-608"},"PeriodicalIF":3.5,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00029g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on breast cancer diagnostic techniques 乳腺癌诊断技术综述

IF 3.5

Sensors & diagnostics Pub Date : 2025-05-19 DOI: 10.1039/D5SD00016E

Parikshana Mathur, Saakshi Dhanekar and B. D. Malhotra

{"title":"A review on breast cancer diagnostic techniques","authors":"Parikshana Mathur, Saakshi Dhanekar and B. D. Malhotra","doi":"10.1039/D5SD00016E","DOIUrl":"https://doi.org/10.1039/D5SD00016E","url":null,"abstract":"Breast cancer occurs when cells grow abnormally and form tumors. It is currently one of the most prevalent cancers in women, and it is known to cause serious detrimental effects if not detected on time. Thus, early detection and screening may tremendously contribute to a patient's medical treatment. The boom in cancer diagnostics resulted from the demand to overcome the limitations of bulky and time-consuming conventional detection methods. The new and advanced methods are simpler, faster and easily deployable. This review elucidates various techniques used for breast cancer detection, which include optical, electrochemical, mechanical, electrical, thermal and color- and breath-based methods. An overview of different techniques is presented with additional information related to the available commercial options. This review also presents the integration of artificial intelligence and Internet of Things into futuristic diagnostic techniques. The unmet needs and challenges are also discussed. Overall, this review is a comprehensive package for researchers who want to dive into the advances of breast cancer diagnostics.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 7","pages":" 555-573"},"PeriodicalIF":3.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00016e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfabricated self-referencing pulstrodes. 微型制造的自参考脉冲。

IF 4.1

Sensors & diagnostics Pub Date : 2025-05-14 eCollection Date: 2025-08-07 DOI: 10.1039/d5sd00024f

Ayian Speck, Davide Migliorelli, Jeremy Disser, Silvia Generelli, Guillaume Bouilly, Tara Forrest, Elena Zdrachek, Loïc Burr, Eric Bakker

{"title":"Microfabricated self-referencing pulstrodes.","authors":"Ayian Speck, Davide Migliorelli, Jeremy Disser, Silvia Generelli, Guillaume Bouilly, Tara Forrest, Elena Zdrachek, Loïc Burr, Eric Bakker","doi":"10.1039/d5sd00024f","DOIUrl":"10.1039/d5sd00024f","url":null,"abstract":"Screen printing and inkjet printing are attractive processes to produce low-cost and mass producible electroanalytical sensors. Despite important advances in the field, obtaining a printed electrochemical reference element that satisfies analytical requirements has not yet been realized satisfactorily. This paper investigates the use of screen printing and inkjet printing to produce a self-contained, all-solid state reference element that can be integrated with a wide range of electroanalytical sensing principles. The principle relies on a silver/silver iodide element that self-generates its potential by the application of a so-called pulstrode protocol. Specifically, a defined quantity of iodide is released by a short cathodic current pulse, and the reference potential defined by the released iodide is subsequently recorded at zero current. Both screen and inkjet-printed reference electrodes are fabricated and characterized, and the methodology optimized and assessed. As an application example, a single-point calibration method is used to quantify ions in undiluted filtered urine samples by potentiometry. The screen-printing approach was less successful owing to the low purity of the silver ink used. The inkjet printing approach allowed one to quantify chloride and sodium in urine. Using a conventional silver/silver chloride reference electrode as standard, relative errors of respectively 7.7 and 14.1% for chloride and sodium were obtained. While the approach would benefit from further optimization for long term applications, especially the use of high purity silver inks, it is a promising strategy for the realization of fully integrated all-solid-state microfabricated sensing systems.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" ","pages":"669-679"},"PeriodicalIF":4.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12132089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bimodal sensor employing a novel approach for simultaneous selective detection of Ni2+ and biomolecules via turn-on fluorescence supported by DFT and molecular docking† 双峰传感器采用一种新的方法，通过DFT和分子对接†支持的开启荧光同时选择性检测Ni2+和生物分子

IF 3.5

Sensors & diagnostics Pub Date : 2025-05-13 DOI: 10.1039/D5SD00028A

Hazeena Shinziya, Avijit Kumar Das, Malavika S Kumar, Anish Nag and Malay Dolai

{"title":"Bimodal sensor employing a novel approach for simultaneous selective detection of Ni2+ and biomolecules via turn-on fluorescence supported by DFT and molecular docking†","authors":"Hazeena Shinziya, Avijit Kumar Das, Malavika S Kumar, Anish Nag and Malay Dolai","doi":"10.1039/D5SD00028A","DOIUrl":"https://doi.org/10.1039/D5SD00028A","url":null,"abstract":"A bimodal sensor, (E)-2-(4-(diphenylamino)styryl)-1-methylquinolin-1-ium (DSM), was designed and synthesized for the simultaneous fluorescence turn-on detection of Ni2+ ion and biomolecules such as ct-DNA, BSA, and ovalbumin. Due to its distinct size and steric properties, DSM exhibits different binding modes when interacting with Ni2+ and DNA/proteins. The probe DSM possesses dual functionalities, allowing it to selectively detect Ni2+ at one binding site while interacting with ct-DNA, BSA, and ovalbumin at another. Thus, interactions of DSM with Ni2+ result in fluorescence enhancement at 377 nm and 400 nm, with a detection limit of 1.53 μM and binding constant of 1.2 × 106 M−1. Moreover, the binding of DSM with Ni2+ has been demonstrated via UV-vis, mass spectra, Jobs plots and DFT analysis. Conversely, binding of DSM with ct-DNA, ovalbumin and BSA led to an increase in the fluorescence at 425 nm and 435 nm, respectively, with the detection limit at micromolar (ct-DNA) and nanomolar (BSA and ovalbumin) levels. These interactions have been validated through UV-vis spectroscopy, fluorescence studies, and molecular docking analysis. Thus, this study underscores the potential of DSM as a versatile tool for simultaneous detection of both metal ions and biomolecules with a unique bimodal approach.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 7","pages":" 622-630"},"PeriodicalIF":3.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00028a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly-sensitive detection of methanol via metal–phenolic film-coated quartz crystal microbalances possessing distinct physicochemical surface profile† 具有不同物理化学表面轮廓†的金属-酚醛膜包覆石英晶体微天平对甲醇的高灵敏度检测

IF 3.5

Sensors & diagnostics Pub Date : 2025-05-09 DOI: 10.1039/D5SD00041F

Karekin D. Esmeryan and Yuliyan Lazarov

{"title":"Highly-sensitive detection of methanol via metal–phenolic film-coated quartz crystal microbalances possessing distinct physicochemical surface profile†","authors":"Karekin D. Esmeryan and Yuliyan Lazarov","doi":"10.1039/D5SD00041F","DOIUrl":"https://doi.org/10.1039/D5SD00041F","url":null,"abstract":"Alcohol is one of the most widespread mind-affecting substances increasing the internal feeling of happiness, euphoria, conviviality and pleasance, but the improperly distilled (adulterated) beverages containing methanol pose risk to the human health. Measures for preventing the intoxication with counterfeit alcohol comprise point-of-use analysis of the alcohol via portable metal-oxide devices, liquid crystal-based detectors and spectrometric sensors with limited effectiveness due to periodic clogging of the separation column, uncertainties induced by the color of emitted light or unstable signal caused by the different optical transparencies of alcohol containers. Contemporary electronics may provide sustainable solutions to these problems by launching miniature metal–phenolic film-coated quartz crystal microbalances (MPF-QCMs) for selective detection of methanol in spirits; however, the effect of surface profile of the sorptive layer on the sensor response of these devices is unknown. We eliminate this knowledge gap by spin coating metal–phenolic films with different morphology, chemistry, wettability and thickness on the surface of six 5 MHz QCMs and subjecting them to the saturated vapor of methanol, ethanol, isopropanol, water, petroleum ether and ammonium hydroxide. The execution of over hundred experiments shows that the MPF-QCMs discriminate the chemical analytes in a repeatable and reproducible manner depending on their molecular size and acidity, and the morphochemical peculiarities of the solid surface, facilitating the registration of methanol fractions up to three orders of magnitude below the admissible concentrations in spirits. Our results provide scientific advance that has potential to address the global challenge related to the consumption of denatured alcohol.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 7","pages":" 609-621"},"PeriodicalIF":3.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00041f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-enzymatic electrochemical assay of N-acetyl-d-neuraminic acid through competitive chemoreceptor binding with (thiophen-3-yl)boronic acid† 通过竞争性化学受体与（噻吩-3-酰基）硼酸结合的n-乙酰-d-神经氨酸的非酶电化学分析

IF 3.5

Sensors & diagnostics Pub Date : 2025-05-08 DOI: 10.1039/D5SD00034C

Athira Tomy, Saurav K. Guin, Conor Cassidy and Eithne Dempsey

{"title":"Non-enzymatic electrochemical assay of N-acetyl-d-neuraminic acid through competitive chemoreceptor binding with (thiophen-3-yl)boronic acid†","authors":"Athira Tomy, Saurav K. Guin, Conor Cassidy and Eithne Dempsey","doi":"10.1039/D5SD00034C","DOIUrl":"https://doi.org/10.1039/D5SD00034C","url":null,"abstract":"Electroanalytical methods which can aid in the selective quantitation of saccharides such as the sialic acid N-acetyl-D-neuraminic acid (Neu5Ac) are very attractive due to their significance in a wealth of human diseases and food/nutritional products. Using cyclic voltammetry, boronic acid–diol recognition based on a redox indicator displacement assay (RIDA) strategy was exploited for non-enzymatic comparative electroanalysis of Neu5Ac vs. fructose using the redox active reporter Alizarin Red S (ARS). The concept has its foundation in the classical competition between an analyte and an indicator (ARS) for the same binding site on a host (boronic acid) molecule. The pH dependent assay employed first-time use of (thiophen-3-yl)boronic acid (TBA) as heterocyclic chemoreceptor. Electrochemistry of ARS in equilibrium with TBA resulted in proton coupled redox processes at −0.48 V (free ARS), −0.29 V (ARS–TBA boronate ester) and +0.51 V vs. Ag|AgCl (free ARS) correlating with ARS concentration in the TBA–ARS equilibrium or in competition equilibrium with a sugar species. Saccharide driven boronic acid displacement resulted in the reinstatement of the free ARS redox processes, forming the basis for the analytical signal. Voltammetry and optical investigations established the optimum conditions for Neu5Ac measurement relative to competing species such as fructose, enabling pH tunable ratiometric quantitation over the range 1–10 mM Neu5Ac (0.1 M sodium acetate buffer pH 5.6) with sensitivity 0.119 ± 0.009 μA mM−1 and LOD 0.63 mM (using differential pulse voltammetry). The homogeneous studies paved the way for film formation and preliminary displacement testing when ARS was surface confined within a chitosan biopolymer layer on a glassy carbon electrode.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 6","pages":" 489-510"},"PeriodicalIF":3.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00034c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mode-directed photopatterning of whispering gallery mode optical resonators 窃窃廊模式光谐振器的模式导向光图纹

IF 3.5

Sensors & diagnostics Pub Date : 2025-05-05 DOI: 10.1039/D5SD00008D

Yuxiang Gao, Vladislav Frenkel, Stephen Arnold and Rastislav Levicky

{"title":"Mode-directed photopatterning of whispering gallery mode optical resonators","authors":"Yuxiang Gao, Vladislav Frenkel, Stephen Arnold and Rastislav Levicky","doi":"10.1039/D5SD00008D","DOIUrl":"https://doi.org/10.1039/D5SD00008D","url":null,"abstract":"Multimodal optical resonators can integrate multiple sensing functions on a single device by assigning specific tasks to different modes. To facilitate such expanded functionality, this study demonstrates a photopatterning approach in which resonantly-amplified light within whispering gallery mode (WGM) sensors is used to direct chemical modification of the corresponding surface region addressed by the mode. A Ru(II) metallo–organic complex containing a caged aminopropyltriethoxysilane (APTES) moiety, [Ru(tpy)(biq)(APTES)](PF6)2, was synthesized and applied as a covalently immobilized layer to solid supports to be patterned, including spheroidal silica WGM resonators. Exciting a mode caused the area exposed to the light to be deprotected, leaving behind a pattern of reactive amine groups available for further derivatization. A two-photon deprotection process enabled the use of near-IR sources for patterning. The photopatterning technique was applied to self-referenced measurements, in which signals from two modes, a sensing and a reference mode, were used to detect specific binding of avidin against a much larger background of nonspecific adsorption. This was accomplished by patterning the sensing mode with biotin to specifically bind avidin while the reference mode tracked nonspecific adsorption.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 6","pages":" 538-546"},"PeriodicalIF":3.5,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d5sd00008d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A label-free nanowell-based impedance sensor for ten-minute SARS-CoV-2 detection† 无标记纳米孔阻抗传感器10分钟检测SARS-CoV-2。

IF 3.5

Sensors & diagnostics Pub Date : 2025-04-30 DOI: 10.1039/D5SD00002E

Zhuolun Meng, Liam White, Pengfei Xie, Hassan Raji, S. Reza Mahmoodi, Aris Karapiperis, Hao Lin, German Drazer, Mehdi Javanmard and Edward P. DeMauro

{"title":"A label-free nanowell-based impedance sensor for ten-minute SARS-CoV-2 detection†","authors":"Zhuolun Meng, Liam White, Pengfei Xie, Hassan Raji, S. Reza Mahmoodi, Aris Karapiperis, Hao Lin, German Drazer, Mehdi Javanmard and Edward P. DeMauro","doi":"10.1039/D5SD00002E","DOIUrl":"10.1039/D5SD00002E","url":null,"abstract":"This work explores label-free biosensing as an effective method for biomolecular analysis, ensuring the preservation of native conformation and biological activity. The focus is on a novel electronic biosensing platform utilizing micro-fabricated nanowell-based impedance sensors, offering rapid, point-of-care diagnosis for SARS-CoV-2 (COVID-19) detection. The nanowell sensor, constructed on a silica substrate through a series of microfabrication processes including deposition, patterning, and etching, features a 5 × 5 well array functionalized with antibodies. Real-time impedance changes within the nanowell array enable diagnostic results within ten minutes using small sample volumes (<5 μL). The research includes assays for SARS-CoV-2 spike proteins in phosphate-buffered saline (PBS) and artificial saliva buffers to mimic real human SARS-CoV-2 samples, covering a wide range of concentrations. The sensor exhibits a detection limit of 0.2 ng mL−1 (1.5 pM) for spike proteins. Middle East respiratory syndrome (MERS-CoV) spike proteins are differentiated from SARS-CoV-2 spike proteins, demonstrating specificity.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 6","pages":" 511-518"},"PeriodicalIF":3.5,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0