Sensors & diagnostics最新文献

{"title":"Dengue-virosensor: advancement of dengue virus-based biosensors","authors":"Mohd. Rahil Hasan, Pradakshina Sharma, Sameer Khan, Ubaid Mushtaq Naikoo, Kapil Bhalla, M. Z. Abdin, Nitesh Malhotra, Tejraj M. Aminabhavi, Nagraj P. Shetti and Jagriti Narang","doi":"10.1039/D4SD00262H","DOIUrl":"https://doi.org/10.1039/D4SD00262H","url":null,"abstract":"<p >Dengue is one of the world's fastest-growing health issues, affecting primarily tropical and sub-tropical countries. Dengue infection is spread <em>via</em> mosquitoes, <em>i.e.</em>, <em>Aedes</em>, and caused by the dengue virus (DENV), a single-stranded RNA virus having 4 serotypes, <em>i.e.</em>, DENV 1–4, and any one of the 4-serotypes can cause dengue fever. Dengue symptoms can range from asymptomatic primary infection to fatal secondary infections, such as dengue shock syndrome (DSS) and dengue hemorrhagic fever (DHF). Since there are no dengue vaccinations or antiviral treatments available, total bed rest, proper hydration, and the use of analgesics for symptomatic pain relief are usually prescribed, which make recovery both difficult and time-consuming. This mandates the development of early detection methods for DENV so that the disease can be stopped before it spreads. For example, traditional laboratory-based diagnostic approaches for detecting dengue infection have one or more flaws. This includes cross-reactivity with other flaviviruses, the need for several samples for serological assays, and the high cost and complexity of PCR processes. Thus, biosensors have garnered considerably greater attention because of their simple fabrication, ease of use, ultra-sensitivity, selectivity, and low cost of production. This review starts with an introduction and a discussion on the conventional methods used for dengue virus detection, along with their limitations. Later, recently developed and futuristic biosensors are summarized before and finally closing this review with a brief conclusion.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 7-23"},"PeriodicalIF":3.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00262h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards greater accountability and trust: the launch of transparent peer review in Sensors & Diagnostics","authors":"","doi":"10.1039/D4SD90043J","DOIUrl":"https://doi.org/10.1039/D4SD90043J","url":null,"abstract":"<p >A graphical abstract is available for this content</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 12","pages":" 1886-1886"},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd90043j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of NIAD-4 derivatives for fluorescence-based detection of protein aggregates†","authors":"Tze Cin Owyong, Laura E. Shippey, Siyang Ding, David S. Owen, Shouxiang Zhang, Jonathan M. White, Wallace W. H. Wong, David P. Smith and Yuning Hong","doi":"10.1039/D4SD00182F","DOIUrl":"https://doi.org/10.1039/D4SD00182F","url":null,"abstract":"<p >Impairment of protein quality control is a critical factor in the development of neurodegenerative disorders like Alzheimer's, Parkinson's and Huntington's disease, characterized by the accumulation of protein aggregates. As such, detection and monitoring of protein aggregates remains a crucial area of study. In this work, we synthesize a series of bithiophene derivatives based on a red emitting amyloid fluorophore NIAD-4. By molecular engineering, widened Stokes shifts and spectral tuning can be achieved in these derivatives. Through molecular docking and aggregation assays, we demonstrate the specificity of these derivatives towards protein fibrils over monomers and amorphous aggregates. Utilizing unbiased flow cytometry together with a cell viability indicator, we show that derivative NIAD-CNOET facilitates the discrimination of cells treated with and without preformed fibrils of α-synuclein, a model of the pathological hallmark of Parkinson's disease.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 55-62"},"PeriodicalIF":3.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00182f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of DNA template sequences to generate fluorescent gold nanoclusters for the sensitive detection of DNA methyltransferase activity bioassay†","authors":"Fangyu Zhou, Hui Chen, Tingting Fan, Zixia Guo, Xiangyan Dong and Feng Liu","doi":"10.1039/D4SD00215F","DOIUrl":"https://doi.org/10.1039/D4SD00215F","url":null,"abstract":"<p >Abnormal DNA methylation mediated by DNA methyltransferases is one of the most common epigenetic modifications, and abnormal DNA methyltransferase activity is often responsible for serious diseases such as cancer. For rapid, sensitive and efficient detection of DNA methyltransferase activity, a bioassay system using gold nanoclusters (DNA-AuNCs) as output has been developed. In this study, dumbbell DNA substrate is recognized and methylated by methyltransferase followed by cleavage by endonuclease (GlaI). In the presence of terminal deoxynucleotidyl transferase (TdT), the poly-A DNA product eventually becomes the template for the reduction of gold nanoclusters and then generated with a strong fluorescence signal. The assay is highly sensitive and requires no amplification or complex material synthesis steps. The detection limit is 0.077 U mL<small>⁻¹</small>. The bioassay showed good detection efficiency in both human serum, cell lysates and human-derived cells. Moreover, it can be used for screening and evaluation of M.SssI MTase inhibitors and hence has great potential use in disease diagnosis and drug discovery. The method was universal and allowed for other biological target detection by simply replacing the sequence of the substrate DNA recognition site; thus the proposed assay has a broad scope of application in both bioassay and drug screening.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 90-101"},"PeriodicalIF":3.5,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00215f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fluorescent sensor array based on carbon dots for the accurate determination of pH†","authors":"Haobo Guo, Pooria Lesani, Hala Zreiqat and Elizabeth J. New","doi":"10.1039/D4SD00275J","DOIUrl":"https://doi.org/10.1039/D4SD00275J","url":null,"abstract":"<p >In this study, we present a sensor array for precise pH monitoring, based on carbon dots (CDs) synthesised from fructose and <em>p</em>-phenylenediamine through a one-step hydrothermal method. The CDs exhibited significant photostability and different fluorescence emissions in different pH conditions, making them suitable for real-time pH sensing across a wide pH range of 3 to 10. Our mechanistic studies revealed how surface functionalisation affects the pH response. For statistical analysis of our array data, we employed Gaussian process regression to create a predictive model for determining pH levels of test samples based on the array spectral data, and linear discriminant analysis for high-precision classification of pH values. This combined approach enabled a comprehensive analysis of the CDs' pH-sensitive fluorescence, demonstrating a significant methodological advancement in pH sensor technology. Our research advances the understanding of the fluorescence mechanisms of CDs in response to pH variations. Furthermore, our study demonstrates the power of integrating machine learning techniques to improve the performance and application scope of fluorescent nanomaterials. These findings have important implications for chemical sensing across a range of fields, including environmental monitoring and biomedical diagnostics.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 12","pages":" 1923-1934"},"PeriodicalIF":3.5,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00275j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: modulation of the binding sites for an adaptable DNA interactive probe: efficient chromo-fluorogenic recognition of Al3+ and live cell bioimaging","authors":"Atanu Maji, Debarpan Mitra, Amitav Biswas, Moumita Ghosh, Rahul Naskar, Saswati Gharami, Nabendu Murmu and Tapan K. Mondal","doi":"10.1039/D4SD90044H","DOIUrl":"https://doi.org/10.1039/D4SD90044H","url":null,"abstract":"<p >Correction for ‘Modulation of the binding sites for an adaptable DNA interactive probe: efficient chromo-fluorogenic recognition of Al<small>³⁺</small> and live cell bioimaging’ by Atanu Maji <em>et al.</em>, <em>Sens. Diagn.</em>, 2024, https://doi.org/10.1039/D4SD00242C</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 12","pages":" 1992-1992"},"PeriodicalIF":3.5,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd90044h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VitalOne™: a point-of-care platform for rapid, comprehensive, central-lab quality blood testing†","authors":"Mounir A. Koussa, Miguel Barreiros, Paul Said Ehrlich Perez, Sae Rin Jean, Taehyung Chris Lee, Ross MacLeod, Aaron Witham, Geeta Bhat, Todd Campbell, Sergio Lizano, Marjorie Toth, Amrita Venkateswaran, Don Yang, Nishat Zaman, Wisam Alfaqheri, Afshan Ardalan, Luis Barbosa, Mehran Behrouzi, Vitali Borisenko, Rohit Chand, Karyn S. Ho, Praveen Kumar, Mate Lengyel, Wei Luo, Fahim Masum, Laura Piñeros, Akhil Rajagopal Kozhipuram, Sergey Sanders, David Santos, Vasu Nadella, Farnoud Kazemzadeh and Iman Khodadad","doi":"10.1039/D4SD00105B","DOIUrl":"https://doi.org/10.1039/D4SD00105B","url":null,"abstract":"<p >Diagnostic tools are fundamental to informed healthcare decisions, but the current state of <em>in vitro</em> diagnostics presents barriers to providing timely access to a wide menu of tests. This paper discusses a method for overcoming these challenges with a novel and miniaturized point-of-care (PoC) solution, the VitalOne. While many PoC solutions have limited test menus tailored for specific scenarios, Vital Bio introduces a comprehensive test menu in a compact PoC format that uses centrifugal microfluidic workflows to deliver quantitative results across three modalities that have traditionally required three separate instruments. VitalOne has combined three modalities into a single instrument: hematology, clinical chemistry, and immunoassay. This breadth of central-lab quality results covers a wide variety of use cases and helps to eliminate the send-out gap that impedes the adoption of PoC technologies. This paper provides a comprehensive and transparent view of both our assay performance data and underlying methods of operation. By comparing the VitalOne system with established benchmarks commercialized devices including Roche cobas® c701, Sysmex 140, Beckman DxH® 500, Beckman AU® 480, and Beckman Access® 2 instrument systems, we demonstrate consistent assay performance, resilience to interference, and performance that is compliant with Clinical Laboratory Improvement Amendments (CLIA) total allowable error standards. Additionally, this article elucidates our system's operational intricacies and showcases performance data from a diverse set of analytes.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 12","pages":" 1899-1922"},"PeriodicalIF":3.5,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00105b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible luminescent Ce3+-sensitized SrF2:Tb3+ for anticounterfeiting and forensic fingerprint detection","authors":"Dhanapriya Devi Yengkhom, Henasurkishore Oinam, Silvia Thongbram, Rajkumar Sunil Singh, Naorem Shanta Singh and Ningombam Yaiphaba","doi":"10.1039/D4SD00277F","DOIUrl":"https://doi.org/10.1039/D4SD00277F","url":null,"abstract":"<p >Ce<small>³⁺</small>-sensitized Tb<small>³⁺</small>-activated SrF<small>₂</small> nanophosphors (NPs) were synthesized using the hydrothermal method. Crystallographic characterization using X-ray diffraction confirms the formation of cubic SrF<small>₂</small> with space group <em>Fm</em><img><em>m</em> for all samples. The FESEM image indicates spherical-shaped particles. Surface functionalization renders that the NPs are dispersible in water. The strong green emission of SrF<small>₂</small>:5% Tb<small>³⁺</small>, 5% Ce<small>³⁺</small> at 541 nm increases by 50-fold than that of SrF<small>₂</small>:5% Tb<small>³⁺</small>. The resonance energy transfer between Ce<small>³⁺</small> and Tb<small>³⁺</small><em>via</em> multipolar interactions is observed. The energy transfer efficiency and spectral overlap integral are calculated. The absolute quantum yield of SrF<small>₂</small>:5% Tb<small>³⁺</small>, 5% Ce<small>³⁺</small> is observed to be ∼12%. The NPs show excellent biocompatibility towards the HeLa cell line with 70% cell viability. Intercellular uptake of the SrF<small>₂</small>:5% Tb<small>³⁺</small>, 5% Ce<small>³⁺</small> nanophosphor is fair, and its potential for anti-counterfeiting and forensic fingerprint applications is observed.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 63-74"},"PeriodicalIF":3.5,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00277f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment of prostate-specific antigen using magnetic-silica antibody nanobioconjugates and fluorescence detection†","authors":"Tumelo Msutu and Philani Mashazi","doi":"10.1039/D4SD00292J","DOIUrl":"https://doi.org/10.1039/D4SD00292J","url":null,"abstract":"<p >Herein, we report the development of an immunosensor for the detection of prostate-specific antigen (PSA). The immunosensor platform was based on the immunometric sandwich protocol, using magnetic-silica nanoparticles for capture and pre-concentration of PSA. The preparation and application of the magnetic-silica nanobioconjugates and the use of phenylboronic acid for the immobilization of the capture antibody are the innovative steps of this report. The fluorescent sensing nanobioprobes contained 6% fluorescein in the fluorescein-doped silica nanoparticles. Silica nanoparticles can easily undergo alkaline dissolution for an enhanced fluorescence signal and thus ultrasensitive detection of PSA. The specificity of the immunosensor was achieved by the use of the anti-PSA monoclonal capture antibody (Ab<small>₁</small>) bioconjugated in an oriented manner onto phenylboronic acid functionalized magnetic-silica nanoparticles. Non-specific binding sites were blocked with glucose to yield Fe<small>₃</small>O<small>₄</small>@SiO<small>₂</small>-PBA-Ab<small>₁</small>/glucose. Ab<small>₁</small> capture magnetic nanoparticles allowed for ease of separation using a magnet. For sensing, the polyclonal anti-PSA antibody (Ab<small>₂</small>) was bioconjugated onto fluorescein-doped silica nanoparticles to form FITC@SiO<small>₂</small>-PBA-Ab<small>₂</small>/glucose. PSA was selectively isolated, enriched and purified from the serum samples using a magnetic nanobioconjugate (Fe<small>₃</small>O<small>₄</small>@SiO<small>₂</small>-PBA-Ab<small>₁</small>/glucose). A sandwich immunoreaction was achieved with FITC@SiO<small>₂</small>-PBA-Ab<small>₂</small>/glucose binding to the captured PSA. The alkali hydrolysis resulted in the disintegration of the nanoparticle thus releasing FITC molecules for fluorescence detection. This resulted in signal amplification. The analytical performance of the proposed immunosensor showed an excellent linear relationship between the fluorescence signal intensity and the concentration of PSA ranging from 2.0 pg mL<small>⁻¹</small> to 100 ng mL<small>⁻¹</small>. The very low limit of detection (LOD) was 0.81 pg mL<small>⁻¹</small> and the limit of quantification (LOQ) was 2.46 pg mL<small>⁻¹</small>. The immunosensor also exhibited good specificity and selectivity to PSA with 98.0–102.7% recovery rates. The detection was accomplished in newborn calf serum samples representing real samples.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 44-54"},"PeriodicalIF":3.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/sd/d4sd00292j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pursuing theranostics: a multimodal architecture approach†","authors":"Aidan A. Bender, Connor K. Holiski, Mary Embree, Heather M. Hennkens, John R. Klaehn, Ellie Lundgreen, Andrew G. Roberts, Peter R. Zalupski and Tara Mastren","doi":"10.1039/D4SD00221K","DOIUrl":"10.1039/D4SD00221K","url":null,"abstract":"<p >Theranostics is a field of nuclear medicine which uses the same targeting vector and chelating system for both a diagnostic and therapeutic radionuclide, allowing for uniformity in imaging and treatment. This growing field requires the development of more flexible chelate systems that permit novel targeting strategies. Toward this end, a multimodal architecture has been realized, making use of a phosphazene-based core and click chemistry to achieve a flexible and customizable scaffold. The six arm phosphazene-based core can scaffold six DTPA chelating motifs or a mixed set of 3 : 3 DTPA : DFO chelates resulting in two multimodal compounds, pDbDt and pDbDtDf, respectively. Terbium complexes displayed strong luminescence, supporting that the structures act as an organic antenna for luminescence. Metal displacement titration studies confirmed the desired structures as well as the capability for heterometallic labeling of the structures. These structures were found to have high thermal and biological stability <em>in vitro</em>. Radiolabeling of each compound resulted in high molar activity labeling of each compound: 169 MBq nmol<small>⁻¹</small>: [<small>¹⁶¹</small>Tb]Tb–pDbDt, 170 MBq nmol<small>⁻¹</small>: [<small>⁸⁹</small>Zr]Zr–pDbDtDf, and the mixed radiolabeling illustrated chelation of both radionuclides in a 1 : 1 ratio. This multimodal architecture is promising as a heterometallic structure for coupling of both a diagnostic and a therapeutic radionuclide with a highly customizable core structure.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 1","pages":" 35-43"},"PeriodicalIF":3.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}