Sensors & diagnostics最新文献_第2页

The importance of antibody orientation for enhancing sensitivity and selectivity in lateral flow immunoassays 抗体定向对提高横向流动免疫测定灵敏度和选择性的重要性

Sensors & diagnostics Pub Date : 2024-08-23 DOI: 10.1039/d4sd00206g

Zhao-Yu Lu, Yang-Hsiang Chan

{"title":"The importance of antibody orientation for enhancing sensitivity and selectivity in lateral flow immunoassays","authors":"Zhao-Yu Lu, Yang-Hsiang Chan","doi":"10.1039/d4sd00206g","DOIUrl":"https://doi.org/10.1039/d4sd00206g","url":null,"abstract":"In the field of point-of-care diagnostics, lateral flow assays (LFAs) stand out as highly promising due to their compact size, ease of use, and rapid analysis times. These attributes make LFAs invaluable, especially in urgent situations or resource-limited regions. However, their Achilles' heel has always been their limited sensitivity and selectivity. To address these issues, various innovative approaches, including sample enrichment, assay optimization, and signal amplification, have been developed and are extensively discussed in the literature. Despite these advancements, the importance of antibody orientation is often neglected, even though improper orientation can significantly impair detection performance. This review article first explores well-established traditional methodologies, such as minor physical adjustments and non-specific chemical bond formations. It then shifts focus to the oriented immobilization of antibodies on probe surfaces. This approach aims to enhance sensitivity and selectivity fundamentally by leveraging protein affinities or complementary amino acid sequences. The review summarizes the impact of antibody orientation on the analytical performance of LFAs in terms of sensitivity, specificity, speed, reliability, cost-effectiveness, and stability. Additionally, we introduce recent modifications to assay membrane materials and discuss the current limitations and future prospects of LFAs.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric nano-biosensor for low-resource settings: insulin as a model biomarker 用于低资源环境的比色纳米生物传感器：以胰岛素为模型生物标记物

Sensors & diagnostics Pub Date : 2024-08-23 DOI: 10.1039/d4sd00197d

Zia ul Quasim Syed, Sathya Samaraweera, Zhuo Wang, Sadagopan Krishnan

{"title":"Colorimetric nano-biosensor for low-resource settings: insulin as a model biomarker","authors":"Zia ul Quasim Syed, Sathya Samaraweera, Zhuo Wang, Sadagopan Krishnan","doi":"10.1039/d4sd00197d","DOIUrl":"https://doi.org/10.1039/d4sd00197d","url":null,"abstract":"Biomarkers provide critical molecular insights into diseases and abnormal conditions. However, detecting them at ultra-low concentrations is a challenge, particularly in areas with limited resources and access to sophisticated instruments. Our research is primarily focused on mitigating this challenge. In this report, we introduce a colorimetric immunosensor for detecting insulin, an essential hormone biomarker that regulates glucose metabolism, at picomolar concentrations using citrate-functionalized magnetic particles. This immunosensor utilizes a two-antibody sandwich immunoassay: one antibody is covalently conjugated to the nanoparticles to capture and isolate the target marker, while the other is labeled with horseradish peroxidase for colorimetric detection of insulin. We conducted comparative analyses of insulin detection in buffer, saliva, and serum samples, offering valuable analytical insights. Our findings indicate a detection limit of 10 pM, with dynamic ranges of 10 pM to 1 nM, 10 pM to 10 nM, and 50 pM to 1 nM for insulin detection in buffer solution, 2-fold diluted serum, and 20-fold diluted artificial saliva, respectively. We demonstrate the application of the color immunosensor to type 1 diabetes and healthy human serum samples. For human saliva analysis, the detection limit needs to be improved in our future studies. Overall, our study enhances biomarker analysis in biofluids through an equipment-free colorimetric method, which is particularly relevant for point-of-need applications.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid-state nanopore counting of amplicons from recombinase polymerase isothermal amplification 对重组酶聚合酶等温扩增产生的扩增子进行固态纳米孔计数

Sensors & diagnostics Pub Date : 2024-08-23 DOI: 10.1039/d4sd00159a

Breeana Elliott, Martin Charron, John Pezacki, Erin McConnell, Vincent Tabard-Cossa

引用次数: 0

In situ interface reaction-enabled electrochemiluminescence imaging for single-cell formaldehyde release analysis† 用于单细胞甲醛释放分析的原位界面反应电化学发光成像†.

IF 3.5

Sensors & diagnostics Pub Date : 2024-08-20 DOI: 10.1039/D4SD00177J

Juanhua Zhou and Yang Liu

{"title":"In situ interface reaction-enabled electrochemiluminescence imaging for single-cell formaldehyde release analysis†","authors":"Juanhua Zhou and Yang Liu","doi":"10.1039/D4SD00177J","DOIUrl":"https://doi.org/10.1039/D4SD00177J","url":null,"abstract":"Monitoring metabolites in situ at the single-cell scale is important for revealing cellular heterogeneity and dynamic changes of cell status, which provides new possibilities for disease research. Benefiting from the advantages of both electrochemical and optical methods, electrochemiluminescence (ECL) has great potential in this field. However, developing real-time in situ imaging methods is full of challenges. In this study, an ECL imaging method for formaldehyde (FA), a kind of cellular metabolite, was developed based on the in situ generation of co-reactants at the electrode interface and was successfully applied to the monitoring of single-cell FA release. Amino groups can undergo a rapid nucleophilic addition reaction with FA to form amino alcohol intermediates, which can be used as co-reactants for tris(2,2′-bipyridyl)ruthenium(II) [Ru(bpy)32+] to significantly enhance the strength of ECL. Poly(amidoamine) (PAMAM), with a large number of amino groups, and reduced graphene oxide (rGO), with excellent electrical conductivity and electrocatalytic properties, were introduced as the modification layer on the electrode surface to realize the “turn on” detection of FA. This sensing method also eliminated the use of the classic toxic co-reactant tripropylamine (TPrA) and was further applied to in situ imaging of single-cell FA release. It successfully obtained ECL images at different time points after the stimulation of HeLa cells with thapsigargin (TG), revealing the change pattern in drug efficacy over time. This work proposes a new ECL imaging approach for real-time in situ monitoring of FA release from single cells, further broadening the application of ECL imaging in single-cell analysis.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00177j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A genetically encoded probe for monitoring and detection of iron in real-time 用于实时监测和检测铁的基因编码探针

Sensors & diagnostics Pub Date : 2024-08-20 DOI: 10.1039/d4sd00091a

Neha Soleja, Mohd. Mohsin

{"title":"A genetically encoded probe for monitoring and detection of iron in real-time","authors":"Neha Soleja, Mohd. Mohsin","doi":"10.1039/d4sd00091a","DOIUrl":"https://doi.org/10.1039/d4sd00091a","url":null,"abstract":"Iron, the most abundant transition metal in the body, regulates cellular function but can be harmful in excess, leading to reactive oxygen species production and cellular damage. Intracellular Fe2+ exerts a significant impact on cellular function, potentially contributing to various critical diseases. To address this, detection methods need high selectivity, sensitivity, and real-time monitoring capabilities, essential for comprehending disease progression. This necessitates advancements beyond conventional detection approaches. Frataxin, a crucial mitochondrial protein, is indispensable for sustaining life, contributing not only to iron metabolism but also to the formation of iron–sulfur clusters critical for cellular function. Its deficiency is implicated in neurodegenerative diseases. We have developed a nanosensor, based on fluorescence resonance energy transfer (FRET), designed to probe iron efflux mechanisms and facilitate dynamic monitoring of iron concentration and its spatial distribution within living cells. To construct this nanosensor, we strategically positioned CyaY, a bacterial frataxin ortholog, between ECFP and Venus, forming a FRET pair. This innovative nanosensor, designated as FeOS (iron optical sensor), demonstrates exceptional selectivity for iron and maintains stability under physiological pH conditions. Additionally, we engineered three mutant variants: I17C, AD10-I17C, and D76H, with A10D-I17C displaying the highest affinity for iron and a broad detection range. The distinguishing feature of this sensor is that it is genetically encoded, facilitating real-time detection of iron levels within living cells.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computationally predicting the performance of gas sensor arrays for anomaly detection 计算预测气体传感器阵列的异常检测性能

Sensors & diagnostics Pub Date : 2024-08-19 DOI: 10.1039/d4sd00121d

Paul Morris, Cory M. Simon

{"title":"Computationally predicting the performance of gas sensor arrays for anomaly detection","authors":"Paul Morris, Cory M. Simon","doi":"10.1039/d4sd00121d","DOIUrl":"https://doi.org/10.1039/d4sd00121d","url":null,"abstract":"In many gas sensing tasks, we simply wish to become aware of gas compositions that deviate from normal, “business-as-usual” conditions. We provide a methodology, illustrated by example, to computationally predict the performance of a gas sensor array design for detecting anomalous gas compositions. Specifically, we consider a sensor array of two zeolitic imidazolate frameworks (ZIFs) as gravimetric sensing elements for detecting anomalous gas compositions in a fruit ripening room. First, we define the probability distribution of the concentrations of the key gas species (CO2, C2H4, H2O) we expect to encounter under normal conditions. Next, we construct a thermodynamic model to predict gas adsorption in the ZIF sensing elements in response to these gas compositions. Then, we generate a synthetic training data set of sensor array responses to “normal” gas compositions. Finally, we train a support vector data description to flag anomalous sensor array responses and test its false alarm and missed-anomaly rates under conceived anomalies. We find the performance of the anomaly detector diminishes with (i) greater variance in humidity, which can mask CO2 and C2H4 anomalies or cause false alarms, (ii) higher levels of noise emanating from the transducers, and (iii) smaller training data sets. Our exploratory study is a step towards computational design of gas sensor arrays for anomaly detection.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nonwoven-fabric-based microfluidic devices for solution viscosity measurements† 用于溶液粘度测量的基于无纺布的微流控装置†。

IF 3.5

Sensors & diagnostics Pub Date : 2024-08-16 DOI: 10.1039/D4SD00188E

Mayumi Otoba Uno, Mariko Omori and Kenji Sakamoto

{"title":"Nonwoven-fabric-based microfluidic devices for solution viscosity measurements†","authors":"Mayumi Otoba Uno, Mariko Omori and Kenji Sakamoto","doi":"10.1039/D4SD00188E","DOIUrl":"https://doi.org/10.1039/D4SD00188E","url":null,"abstract":"Microfluidic chips designed to measure viscosity with extremely small amounts of liquids are expected to examine biological fluids, such as for the prediction of disease states and stress assessment, and for the evaluation of the physical properties of novel synthetic materials. However, these devices typically require sample volumes of several tens of μL or more, which has limitations when collecting biological samples from individuals nearly non-invasively. In this study, we fabricated a flow channel on a nonwoven fabric substrate with tailored hydrophilic and hydrophobic properties to enable viscosity measurements with the small-volume flow of aqueous solutions, such as 3 μL of saline. By measuring the electrical conductivity of the liquid using comb-shaped printed electrodes in contact with the flow path, we quantified the time and distance of liquid flow driven by capillary action to estimate solution viscosity. Using a mixture of glycerol and saline solution with varying viscosities, while maintaining a constant ion concentration, we demonstrated the capability to assess the relative viscosity of solutions. This was achieved by evaluating the correlation coefficient between the flow time and distance, and the net electrical conductivity, which is influenced by the viscosity and ion concentration of the solutions. This study lays the groundwork for developing a low-cost technique to measure the viscosity of solutions with a few μL, offering potential for routine health monitoring and disease prevention.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00188e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection of DNA aptamers for sensing drugs treating eye disease: atropine and timolol maleate 选择用于感知治疗眼疾药物的 DNA 合体：阿托品和马来酸噻吗洛尔

Sensors & diagnostics Pub Date : 2024-08-15 DOI: 10.1039/d4sd00223g

Ka-Ying Wong, Yibo Liu, Chau-Minh Phan, Lyndon Jones, Man-Sau Wong, Juewen Liu

引用次数: 0

Antibody conjugates as CT/MRI Theranostics for diagnosis of cancers: a review of recent trends and advances 用于癌症诊断的 CT/MRI Theranostics 抗体共轭物：最新趋势和进展综述

IF 3.5

Sensors & diagnostics Pub Date : 2024-08-12 DOI: 10.1039/D4SD00132J

Saba Abaei, Ali Tarighatnia, Asghar Mesbahi and Ayuob Aghanejad

{"title":"Antibody conjugates as CT/MRI Theranostics for diagnosis of cancers: a review of recent trends and advances","authors":"Saba Abaei, Ali Tarighatnia, Asghar Mesbahi and Ayuob Aghanejad","doi":"10.1039/D4SD00132J","DOIUrl":"https://doi.org/10.1039/D4SD00132J","url":null,"abstract":"The constant need for cancer diagnosis in the early stages drives the development of contrast agents and imaging methods. Imaging agents have important roles in monitoring the progression and metastasis of cancers. Antibodies as biomolecules in conjugation with nanoparticles, radioisotopes, and drugs have been used as biomarkers for the early diagnosis/therapy of cancers due to their serum stability, affinity, and specificity. While antibodies are commonly used as nuclear medicine biomarkers, antibody-based contrast agent platforms have recently gained attention in X-ray computed tomography (CT) and magnetic resonance imaging (MRI). The developing antibody-based contrast agents have revolutionized cancer imaging techniques, particularly through MRI. Despite the promising advancements, some challenges and limitations need to be addressed for the extensive applications of these agents. Ongoing research is focused on overcoming challenges and limitations to enhance the efficiency and accuracy of these imaging methods. With continued advancements, antibody-based contrast agents hold immense potential in the early diagnosis and treatment of cancer. In this review, we summarize and categorize the recent progress in targeted imaging using antibody-based contrast agents by MRI and CT modalities.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00132j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wearable Strain Sensors: Design Shapes, Fabrication, Encapsulation and Performance Evaluation Methods 可穿戴式应变传感器：设计形状、制造、封装和性能评估方法

Sensors & diagnostics Pub Date : 2024-08-09 DOI: 10.1039/d4sd00190g

Nur Nazihah Abu Hassan Zahri, A. N. Nordin, Norsinnira Zainul Azlan, Ibrahim Hafizu Hassan, Lun Hao Tung, Lai Ming Lim, Zambri Samsudin

{"title":"Wearable Strain Sensors: Design Shapes, Fabrication, Encapsulation and Performance Evaluation Methods","authors":"Nur Nazihah Abu Hassan Zahri, A. N. Nordin, Norsinnira Zainul Azlan, Ibrahim Hafizu Hassan, Lun Hao Tung, Lai Ming Lim, Zambri Samsudin","doi":"10.1039/d4sd00190g","DOIUrl":"https://doi.org/10.1039/d4sd00190g","url":null,"abstract":"Highly durable, stretchable, sensitive and biocompatible wearable strain sensors are crucial for healthcare, sports, and robotics applications. While strain sensor designs, fabrication and performance evaluation methods have been widely discussed by researchers, not many have discussed sensor improvements via implementing designs and protection layers that make the sensor more resilient. This paper will focus on sensor designs (straight-line, U-shape, serpentine, and Kirigami) and material selection that can provide better performance. Theoretical equations and calculations to indicate how the design shapes contribute to providing better performance are also included. An important aspect which is not often explored is having encapsulation layers which can significantly to reduce the formation of cracks when the sensor subjected to mechanical stress and bending. This review will include post-fabrication steps that are necessary to incorportate protection layers for the wearable sensors. Due to the curvilinear shapes of wearable sensors that often need to be in close contact with human skin, reliability and durability testing often differs greatly from traditional strain sensors. Recent techniques for performance evaluation specific to wearable sensors such as cyclic stretching, bending, stretch till failure, washability, signal latency, tensile tests were also discussed in detail. This includes experimental setup, duration of testing and its significance were described. To ensure device safety for the user, biocompatibility assessments need to be made. In this review, cytotoxicity test methods such as trypan blue, cell proliferation and MTT assay were compared and evaluated. By consolidating recent developments, this paper aims to provide researchers and practitioners with a comprehensive understanding of the advancements, and future directions in this rapidly evolving field.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141944543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0