Sensors & diagnostics最新文献_第3页

3D-printed electrochemical cells for multi-point aptamer-based drug measurements† 三维打印电化学电池用于基于多点色聚体的药物测量

IF 3.5

Sensors & diagnostics Pub Date : 2024-08-08 DOI: 10.1039/D4SD00192C

John Mack, Raygan Murray, Kenedi Lynch and Netzahualcóyotl Arroyo-Currás

{"title":"3D-printed electrochemical cells for multi-point aptamer-based drug measurements†","authors":"John Mack, Raygan Murray, Kenedi Lynch and Netzahualcóyotl Arroyo-Currás","doi":"10.1039/D4SD00192C","DOIUrl":"10.1039/D4SD00192C","url":null,"abstract":"Electrochemical aptamer-based (E-AB) sensors achieve detection and quantitation of biomedically relevant targets such as small molecule drugs and protein biomarkers in biological samples. E-ABs are usually fabricated on commercially available macroelectrodes which, although functional for rapid sensor prototyping, can be costly and are not compatible with the microliter sample volumes typically available in biorepositories for clinical validation studies. Seeking to develop a multi-point sensing platform for sensor validation in sample volumes characteristic of clinical studies, we report a protocol for in-house assembly of 3D-printed E-ABs. We employed a commercially available 3D stereolithographic printer (FormLabs, $5k USD) for electrochemical cell fabrication and directly embedded electrodes within the 3D-printed cell structure. This approach offers a reproducible and reusable electrode fabrication process resulting in four independent and simultaneous measurements for statistically weighted results. We demonstrate compatibility with aptamer sequences binding antibiotics and antineoplastic agents. We also demonstrate a proof-of-concept validation of serum vancomycin measurements using clinical samples. Our results demonstrate that 3D-printing can be used in conjunction with E-ABs for accessible, rapid, and statistically meaningful validation of E-AB sensors in biological matrices.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00192c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141944595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fast and highly selective ECL creatinine sensor for diagnosis of chronic kidney disease† 用于诊断慢性肾病的快速、高选择性 ECL 肌酐传感器

IF 3.5

Sensors & diagnostics Pub Date : 2024-08-07 DOI: 10.1039/D4SD00165F

Hosein Afshary and Mandana Amiri

{"title":"A fast and highly selective ECL creatinine sensor for diagnosis of chronic kidney disease†","authors":"Hosein Afshary and Mandana Amiri","doi":"10.1039/D4SD00165F","DOIUrl":"10.1039/D4SD00165F","url":null,"abstract":"Monitoring of creatinine in human fluid has attracted considerable attention owing to the potential for diagnosis of chronic kidney disease. However, the detection of creatinine has been difficult owing to its electrochemical and optical inertness. In this approach, a highly selective and sensitive electrochemiluminescence (ECL) strategy based on homogeneous carbon quantum dots (CQDs) for the detection of creatinine was introduced. A copper(II) picrate complex was added at the surface of electrode to improve the selectivity of the sensor significantly by the formation of a Janovsky complex. A multi-pulse amperometric technique was applied as a very fast and reliable method for quantitative determination of creatinine. The calibration curve was acquired with a linear range from 1.0 × 10−8 to 1 × 10−5 M with a low detection limit of 8.7 × 10−9 M. The proposed creatinine sensing platform is experimentally very simple and shows high selectivity with a broad linear range of detection. Furthermore, the presented method can determine creatinine in real samples with excellent recoveries.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00165f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141944545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Competitive Horseradish Peroxidase-Linked Aptamer Assay for Sensitive Detection of 17β-Estradiol with a New Aptamer 利用新型色聚体灵敏检测 17β 雌二醇的竞争性辣根过氧化物酶标记色聚体分析法

Sensors & diagnostics Pub Date : 2024-08-07 DOI: 10.1039/d4sd00208c

Qiuyi Cheng, Qiang Zhao

{"title":"Competitive Horseradish Peroxidase-Linked Aptamer Assay for Sensitive Detection of 17β-Estradiol with a New Aptamer","authors":"Qiuyi Cheng, Qiang Zhao","doi":"10.1039/d4sd00208c","DOIUrl":"https://doi.org/10.1039/d4sd00208c","url":null,"abstract":"17β-estradiol (E2) is one of typical endocrine disrupting compounds (EDCs), which plays a major role in facilitating the growth and regulating the balance of human endocrine system. E2 contamination can cause environment and health risks as E2 exposure can interfere endocrine system by binding to estrogen receptors. It is imperative to develop sensitive methods for E2 detection. Here we developed a competitive enzyme-linked aptamer assay for E2 detection by using a newly reported high-affinity DNA aptamer as affinity ligand. The complementary DNA (cDNA) of anti-E2 aptamer is conjugated on microplate. Horseradish peroxidase (HRP) is labeled on aptamer probe. In the absence of E2, HRP-labeled aptamer is captured by cDNA, and HRP catalyzes substrate into product, generating absorbance signal or chemiluminescence signal. In the presence of E2, E2 binds with aptamer, causing displacement of HRP-labeled aptamer from microplate and decrease of signals. In absorbance-analysis mode, the detection limit of E2 reached 0.2 nmol/L with a dynamic range from 0.2 nmol/L to 20 μmol/L. In chemiluminescence-analysis mode, this method enabled the quantification of E2 at 50 pmol/L, with a dynamic range from 50 pmol/L to 50 μmol/L. This method could also detect E2 spiked in lake water sample, showing promise in practical applications.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141944544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paper integrated microfluidic contact lens for colorimetric glucose detection 用于比色葡萄糖检测的纸质集成微流体接触镜

Sensors & diagnostics Pub Date : 2024-08-05 DOI: 10.1039/d4sd00135d

Pelin Kubra Isgor, Taher Abbasiasl, Ritu Das, Emin Istif, Umut Can Yener, Levent Beker

{"title":"Paper integrated microfluidic contact lens for colorimetric glucose detection","authors":"Pelin Kubra Isgor, Taher Abbasiasl, Ritu Das, Emin Istif, Umut Can Yener, Levent Beker","doi":"10.1039/d4sd00135d","DOIUrl":"https://doi.org/10.1039/d4sd00135d","url":null,"abstract":"Contact lenses offer a simple, cost-effective, and noninvasive method for in-situ real-time analysis of various biomarkers. Electro-chemical sensors are integrated into contact lenses for analysis of various biomarkers. However, they suffer from rigid electronic components and connections, leading to eye irritation and biomarker concentration deviation. Here, a flexible, and microfluidic integrated paper-based contact lens for colorimetric analysis of glucose was implemented. Facilitating a three-dimensional (3D) printer for lens fabrication eliminates cumbersome cleanroom process and provides a simple, batch compatible process. Due to the capillary force of the filter paper, the sample was routed to detection chambers inside microchannels, and it allowed further colorimetric detection. Paper-embedded microfluidic contact lens successfully detects glucose down to 2 mM within ~10 sec. The small dimension of the microfluidic system enables detection of glucose levels as low as 5 µl. The results show the potential of the presented approach to analyze glucose concentration in a rapid manner. It is demonstrated that the fabricated contact lens can successfully detect glucose levels of diabetic patients.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141944594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Dual State Emission Luminogen based on 1,3,3-trimethylindoline and chroman-2,4-dione Conjugate for Highly Selective Dual Channel Detection of Cyanide Ion 一种基于 1,3,3-三甲基吲哚啉和色烷-2,4-二酮共轭物的双态发射光源，用于高选择性双通道检测氰离子

Sensors & diagnostics Pub Date : 2024-08-02 DOI: 10.1039/d4sd00155a

Snehadrinarayan Khatua, Sumit Kumar Patra, Monosh Rabha, Deikrisha Lyngdoh Lyngkhoi, Jogat Gogoi, Bhaskar Sen

{"title":"A Dual State Emission Luminogen based on 1,3,3-trimethylindoline and chroman-2,4-dione Conjugate for Highly Selective Dual Channel Detection of Cyanide Ion","authors":"Snehadrinarayan Khatua, Sumit Kumar Patra, Monosh Rabha, Deikrisha Lyngdoh Lyngkhoi, Jogat Gogoi, Bhaskar Sen","doi":"10.1039/d4sd00155a","DOIUrl":"https://doi.org/10.1039/d4sd00155a","url":null,"abstract":"Dual state emission luminogen (DSEgen) with strong fluorescence in both solution and solid states has extensive potential for numerous applications. Herein, a chroman-2,4-dione and indoline conjugate, 2 was synthesized for highly selective and sensitive turn-on fluorescent detection of cyanide ion. Compound 2 behaves as a molecular rotor and shows the dual state emission (DSE) phenomenon and multicolour emission. It displays bright fluorescence in both the concentrated solution and the solid-state. The compound is nonfluorescent in dilute solution, and with increasing concentration, it shows aggregation caused red-shifted emission. With increasing concentration, the emission colour changes from green to yellow to orange-red. The CC bond attached to the coumarin moiety is a compelling target for nucleophilic addition. Cyanide ions reacted with the probe and remarkably changed spectroscopic properties. With the gradual addition of cyanide, the colour of the probe solution was changed from yellow to colorless. The very weakly emissive probe 2 rapidly reacted with CN− and emitted strongly due to the inhibition of internal charge transfer (ICT) from indoline to chroman-2,4-dione. The DSEgen properties and CN sensing were thoroughly investigated and supported using spectroscopic studies, TDDFT, and single-crystal X-ray diffraction.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of solvents, electrolytes, and mediators for polyindole-based electrochemical sensors 基于聚吲哚的电化学传感器

IF 3.5

Sensors & diagnostics Pub Date : 2024-08-02 DOI: 10.1039/D4SD00175C

P. C. Pandey, Atul Kumar Tiwari and Roger J. Narayan

引用次数: 0

Electrochemical detection of tumor cells based on proximity labelling-assisted multiple signal amplification† 基于近距离标记辅助多重信号放大技术的肿瘤细胞电化学检测技术

IF 3.5

Sensors & diagnostics Pub Date : 2024-08-02 DOI: 10.1039/D4SD00217B

Guozhang Zhou, Fei Zhou, Xiaomeng Yu, Daiyuan Zhou, Jiaqi Wang, Bing Bo, Ya Cao and Jing Zhao

{"title":"Electrochemical detection of tumor cells based on proximity labelling-assisted multiple signal amplification†","authors":"Guozhang Zhou, Fei Zhou, Xiaomeng Yu, Daiyuan Zhou, Jiaqi Wang, Bing Bo, Ya Cao and Jing Zhao","doi":"10.1039/D4SD00217B","DOIUrl":"10.1039/D4SD00217B","url":null,"abstract":"Malignant tumors are the second leading cause of human deaths worldwide, and early cancer screening and diagnosis can effectively reduce cancer mortality. Herein, we propose a new electrochemical method for the highly sensitive detection of MUC1-positive tumor cells based on proximity labelling-assisted multiple signal amplification. Specifically, a MUC1 aptamer-modified electrode was prepared for capturing MUC1-positive tumor cells, followed by binding of G4-DNA strands to the cells with the aid of a mild reduction reaction. A hemin/G4-DNA complex was then formed and acted as a mimic of horseradish peroxidase, catalysing the proximal labelling of tyramine-modified gold nanoparticles to induce silver-enhanced electrochemical signal amplification. Electrochemical results demonstrated that the method was able to specially identify MUC1-positive tumor cells and generate corresponding electrochemical responses in the range of 100 cells per mL to 1 × 106 cells per mL with a detection limit of 21 cells per mL. Furthermore, the method displayed good stability and anti-interference performance in complex serum environments. Therefore, our work may provide an effective tool to improve the accuracy of cell-based tissue examination and liquid biopsy for early diagnosis of cancers in the future.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00217b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct writing of graphene electrodes for point-of-care electrochemical sensing applications 直接写入石墨烯电极用于护理点电化学传感应用

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-31 DOI: 10.1039/D4SD00140K

Lei Zhao, Andrew Piper, Giulio Rosati and Arben Merkoçi

引用次数: 0

Synthesis and characterization of La QDs: sensors for anions and H2O2† La QDs 的合成与表征：阴离子和 H2O2 传感器

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-26 DOI: 10.1039/D4SD00142G

Amit Sahoo and Achyuta N. Acharya

{"title":"Synthesis and characterization of La QDs: sensors for anions and H2O2†","authors":"Amit Sahoo and Achyuta N. Acharya","doi":"10.1039/D4SD00142G","DOIUrl":"10.1039/D4SD00142G","url":null,"abstract":"The development of sensitive and accurate fluorescence sensors for the detection of anions and reactive oxygen species (ROS, H2O2) is essential as they play significant roles in biological and chemical processes. In this work, semiconductor La QDs were synthesized. The synthesized La QDs were determined to be pure with 100% La element using EDS technique. La QDs were observed in both cubic and hexagonal lattice configurations through powder XRD analysis. The morphology of the La QDs was characterized using HRTEM and FESEM data as tiny, spherical, homogenous QDs with a diameter ranging from 2 to 6 nm. The fluorescence characteristics of the synthesized La QDs were examined by studying their sensing properties that increased with an increase in anion concentration and decreased with an increase in [H2O2]. The variation in emission intensity at 315 nm and 440.5 nm satisfied the Stern–Volmer equation. The LOD and LOQ of H2O2 and anion sensing with La QDs were studied in the μM range. The Langmuir binding plots and FTIR spectra supported the concept that the surface functionalization of La QDs occurred in the presence of anions. With two band gap energies of about 3.26 eV and 4.66 eV, the synthesized La QDs are a mixture of two (binary) semiconductors.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00142g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized gadolinium-DO3A loading in RAFT-polymerized copolymers for superior MR imaging of aging blood–brain barrier† 优化 RAFT 聚合共聚物中的钆-DOTA 负载，为老化血脑屏障提供优质磁共振成像

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-25 DOI: 10.1039/D4SD00063C

Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest M. Kievit and Anthony J. Convertine

{"title":"Optimized gadolinium-DO3A loading in RAFT-polymerized copolymers for superior MR imaging of aging blood–brain barrier†","authors":"Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest M. Kievit and Anthony J. Convertine","doi":"10.1039/D4SD00063C","DOIUrl":"10.1039/D4SD00063C","url":null,"abstract":"The development of gadolinium-based contrast agents (GBCAs) has been pivotal in advancing magnetic resonance imaging (MRI), offering enhanced soft tissue contrast without ionizing radiation exposure. Despite their widespread clinical use, the need for improved GBCAs has led to innovations in ligand chemistry and polymer science. We report a novel approach using methacrylate-functionalized DO3A ligands to synthesize a series of copolymers through direct reversible addition-fragmentation chain transfer (RAFT) polymerization. This technique enables precise control over the gadolinium content within the polymers, circumventing the need for subsequent conjugation and purification steps, and facilitates the addition of other components such as targeting ligands. The resulting copolymers were analysed for their relaxivity properties, indicating that specific gadolinium-DO3A loading contents between 12–30 mole percent yield optimal MRI contrast enhancement. Inductively coupled plasma (ICP) measurements corroborated these findings, revealing a non-linear relationship between gadolinium content and relaxivity. Optimized copolymers were synthesized with the claudin-1 targeting peptide, C1C2, to image BBB targeting in aged mice to show imaging utility. This study presents a promising pathway for the development of more efficient GBCA addition to copolymers for targeted drug delivery and bioimaging application.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00063c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0