Sensors & diagnostics最新文献

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A dual state emission luminogen based on the 1,3,3-trimethylindoline and chroman-2,4-dione conjugate for highly selective dual channel detection of cyanide ions† 一种基于 1,3,3-三甲基吲哚啉和色烷-2,4-二酮共轭物的双态发射光源,用于高选择性双通道检测氰离子
IF 3.5
Sensors & diagnostics Pub Date : 2024-08-02 DOI: 10.1039/D4SD00155A
Snehadrinarayan Khatua, Sumit Kumar Patra, Monosh Rabha, Deikrisha Lyngdoh Lyngkhoi, Jogat Gogoi and Bhaskar Sen
{"title":"A dual state emission luminogen based on the 1,3,3-trimethylindoline and chroman-2,4-dione conjugate for highly selective dual channel detection of cyanide ions†","authors":"Snehadrinarayan Khatua, Sumit Kumar Patra, Monosh Rabha, Deikrisha Lyngdoh Lyngkhoi, Jogat Gogoi and Bhaskar Sen","doi":"10.1039/D4SD00155A","DOIUrl":"10.1039/D4SD00155A","url":null,"abstract":"<p >Dual state emission luminogens (DSEgens) with strong fluorescence in both solution and solid states have extensive potential for numerous applications. Herein, a chroman-2,4-dione and indoline conjugate, <strong>2</strong>, was synthesized for highly selective and sensitive turn-on fluorescent detection of cyanide ions. Compound <strong>2</strong> behaves as a molecular rotor and shows the dual state emission (DSE) phenomenon and multicolour emission. It displays bright fluorescence in both the concentrated solution and the solid-state. The compound is nonfluorescent in dilute solution, and with increasing concentration, it shows aggregation caused red-shifted emission. With increasing concentration, the emission colour changes from green to yellow to orange-red. The C<img>C bond attached to the indoline moiety is a compelling target for nucleophilic addition. Cyanide ions reacted with the probe which remarkably changed the spectroscopic properties. With the gradual addition of cyanide, the colour of the probe solution was changed from yellow to colorless. The very weakly emissive probe <strong>2</strong> rapidly reacted with CN<small><sup>−</sup></small> and emitted strongly due to the inhibition of internal charge transfer (ICT) from indoline to chroman-2,4-dione. The DSEgen properties and CN sensing were thoroughly investigated and supported using spectroscopic studies, TDDFT, and single-crystal X-ray diffraction.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 10","pages":" 1689-1698"},"PeriodicalIF":3.5,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00155a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of solvents, electrolytes, and mediators for polyindole-based electrochemical sensors 基于聚吲哚的电化学传感器
IF 3.5
Sensors & diagnostics Pub Date : 2024-08-02 DOI: 10.1039/D4SD00175C
P. C. Pandey, Atul Kumar Tiwari and Roger J. Narayan
{"title":"Optimization of solvents, electrolytes, and mediators for polyindole-based electrochemical sensors","authors":"P. C. Pandey, Atul Kumar Tiwari and Roger J. Narayan","doi":"10.1039/D4SD00175C","DOIUrl":"10.1039/D4SD00175C","url":null,"abstract":"<p >Surface-engineered conducting polymers (CPs) have enabled technological advances in chemistry and materials science. Heterocyclic conjugated organic molecules, specifically indole and its derivatives, have the potential to be polymerized under electrochemically controlled conditions in different types of compatible solvent media, including self-assembled nanofluids, for several applications. Polymer-based electrode materials are valuable for the detection of various targeted biomolecules and other analytes. This review outlines the evolution of the electropolymerization technique in recent years, along with developments in the field. With advances in nanoscience, several materials have been used to modify CPs for electrochemical sensing. Several biomedical applications and the role of antifouling agents in the properties of several electropolymerized thin films are highlighted.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1392-1405"},"PeriodicalIF":3.5,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00175c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141886929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrochemical detection of tumor cells based on proximity labelling-assisted multiple signal amplification† 基于近距离标记辅助多重信号放大技术的肿瘤细胞电化学检测技术
IF 3.5
Sensors & diagnostics Pub Date : 2024-08-02 DOI: 10.1039/D4SD00217B
Guozhang Zhou, Fei Zhou, Xiaomeng Yu, Daiyuan Zhou, Jiaqi Wang, Bing Bo, Ya Cao and Jing Zhao
{"title":"Electrochemical detection of tumor cells based on proximity labelling-assisted multiple signal amplification†","authors":"Guozhang Zhou, Fei Zhou, Xiaomeng Yu, Daiyuan Zhou, Jiaqi Wang, Bing Bo, Ya Cao and Jing Zhao","doi":"10.1039/D4SD00217B","DOIUrl":"10.1039/D4SD00217B","url":null,"abstract":"<p >Malignant tumors are the second leading cause of human deaths worldwide, and early cancer screening and diagnosis can effectively reduce cancer mortality. Herein, we propose a new electrochemical method for the highly sensitive detection of MUC1-positive tumor cells based on proximity labelling-assisted multiple signal amplification. Specifically, a MUC1 aptamer-modified electrode was prepared for capturing MUC1-positive tumor cells, followed by binding of G4-DNA strands to the cells with the aid of a mild reduction reaction. A hemin/G4-DNA complex was then formed and acted as a mimic of horseradish peroxidase, catalysing the proximal labelling of tyramine-modified gold nanoparticles to induce silver-enhanced electrochemical signal amplification. Electrochemical results demonstrated that the method was able to specially identify MUC1-positive tumor cells and generate corresponding electrochemical responses in the range of 100 cells per mL to 1 × 10<small><sup>6</sup></small> cells per mL with a detection limit of 21 cells per mL. Furthermore, the method displayed good stability and anti-interference performance in complex serum environments. Therefore, our work may provide an effective tool to improve the accuracy of cell-based tissue examination and liquid biopsy for early diagnosis of cancers in the future.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1542-1550"},"PeriodicalIF":3.5,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00217b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Direct writing of graphene electrodes for point-of-care electrochemical sensing applications 直接写入石墨烯电极用于护理点电化学传感应用
IF 3.5
Sensors & diagnostics Pub Date : 2024-07-31 DOI: 10.1039/D4SD00140K
Lei Zhao, Andrew Piper, Giulio Rosati and Arben Merkoçi
{"title":"Direct writing of graphene electrodes for point-of-care electrochemical sensing applications","authors":"Lei Zhao, Andrew Piper, Giulio Rosati and Arben Merkoçi","doi":"10.1039/D4SD00140K","DOIUrl":"10.1039/D4SD00140K","url":null,"abstract":"<p >Electrochemical sensors are increasingly garnering attention as valuable tools for point-of-care (POC) testing due to their low costs, high sensitivities, and ease of miniaturization. Graphene-based materials, renowned for their tunable electrical conductivity, high specific surface areas, versatile functionality, and biocompatibility; are highly suited for the fabrication of electrochemical sensors with heightened sensitivities. Non-contact, maskless, direct writing methods allow the rapid, large-scale production of graphene electrodes with high design flexibility. Researchers globally are advancing graphene electrode production, aiming for smaller, faster, and more efficient sensors. This review provides a comprehensive overview of recent advances on the direct writing of graphene electrodes for electrochemical sensing applications. It covers the basics of direct writing techniques, the advancements in graphene ink/precursor preparation, structural design, and device integration, with a focus on POC platforms.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1406-1427"},"PeriodicalIF":3.5,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00140k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141867834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis and characterization of La QDs: sensors for anions and H2O2† La QDs 的合成与表征:阴离子和 H2O2 传感器
IF 3.5
Sensors & diagnostics Pub Date : 2024-07-26 DOI: 10.1039/D4SD00142G
Amit Sahoo and Achyuta N. Acharya
{"title":"Synthesis and characterization of La QDs: sensors for anions and H2O2†","authors":"Amit Sahoo and Achyuta N. Acharya","doi":"10.1039/D4SD00142G","DOIUrl":"10.1039/D4SD00142G","url":null,"abstract":"<p >The development of sensitive and accurate fluorescence sensors for the detection of anions and reactive oxygen species (ROS, H<small><sub>2</sub></small>O<small><sub>2</sub></small>) is essential as they play significant roles in biological and chemical processes. In this work, semiconductor La QDs were synthesized. The synthesized La QDs were determined to be pure with 100% La element using EDS technique. La QDs were observed in both cubic and hexagonal lattice configurations through powder XRD analysis. The morphology of the La QDs was characterized using HRTEM and FESEM data as tiny, spherical, homogenous QDs with a diameter ranging from 2 to 6 nm. The fluorescence characteristics of the synthesized La QDs were examined by studying their sensing properties that increased with an increase in anion concentration and decreased with an increase in [H<small><sub>2</sub></small>O<small><sub>2</sub></small>]. The variation in emission intensity at 315 nm and 440.5 nm satisfied the Stern–Volmer equation. The LOD and LOQ of H<small><sub>2</sub></small>O<small><sub>2</sub></small> and anion sensing with La QDs were studied in the μM range. The Langmuir binding plots and FTIR spectra supported the concept that the surface functionalization of La QDs occurred in the presence of anions. With two band gap energies of about 3.26 eV and 4.66 eV, the synthesized La QDs are a mixture of two (binary) semiconductors.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1476-1493"},"PeriodicalIF":3.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00142g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimized gadolinium-DO3A loading in RAFT-polymerized copolymers for superior MR imaging of aging blood–brain barrier† 优化 RAFT 聚合共聚物中的钆-DOTA 负载,为老化血脑屏障提供优质磁共振成像
IF 3.5
Sensors & diagnostics Pub Date : 2024-07-25 DOI: 10.1039/D4SD00063C
Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest M. Kievit and Anthony J. Convertine
{"title":"Optimized gadolinium-DO3A loading in RAFT-polymerized copolymers for superior MR imaging of aging blood–brain barrier†","authors":"Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest M. Kievit and Anthony J. Convertine","doi":"10.1039/D4SD00063C","DOIUrl":"10.1039/D4SD00063C","url":null,"abstract":"<p >The development of gadolinium-based contrast agents (GBCAs) has been pivotal in advancing magnetic resonance imaging (MRI), offering enhanced soft tissue contrast without ionizing radiation exposure. Despite their widespread clinical use, the need for improved GBCAs has led to innovations in ligand chemistry and polymer science. We report a novel approach using methacrylate-functionalized DO3A ligands to synthesize a series of copolymers through direct reversible addition-fragmentation chain transfer (RAFT) polymerization. This technique enables precise control over the gadolinium content within the polymers, circumventing the need for subsequent conjugation and purification steps, and facilitates the addition of other components such as targeting ligands. The resulting copolymers were analysed for their relaxivity properties, indicating that specific gadolinium-DO3A loading contents between 12–30 mole percent yield optimal MRI contrast enhancement. Inductively coupled plasma (ICP) measurements corroborated these findings, revealing a non-linear relationship between gadolinium content and relaxivity. Optimized copolymers were synthesized with the claudin-1 targeting peptide, C1C2, to image BBB targeting in aged mice to show imaging utility. This study presents a promising pathway for the development of more efficient GBCA addition to copolymers for targeted drug delivery and bioimaging application.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1513-1521"},"PeriodicalIF":3.5,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00063c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of surfactants using a hydrophobic natural deep eutectic solvent and smartphone† 使用疏水性天然深共晶溶剂和智能手机检测表面活性剂
IF 3.5
Sensors & diagnostics Pub Date : 2024-07-24 DOI: 10.1039/D4SD00196F
Vagner Bezerra dos Santos, Lucas B. Ayres, Helayne Santos de Sousa, Carlos D. Garcia and Willian Toito Suarez
{"title":"Detection of surfactants using a hydrophobic natural deep eutectic solvent and smartphone†","authors":"Vagner Bezerra dos Santos, Lucas B. Ayres, Helayne Santos de Sousa, Carlos D. Garcia and Willian Toito Suarez","doi":"10.1039/D4SD00196F","DOIUrl":"10.1039/D4SD00196F","url":null,"abstract":"<p >We report on the advantages of a green method to detect surfactants in environmental water samples. The approach is based on the use of a hydrophobic natural deep eutectic solvent (NADES) to extract the complexes formed by the surfactants and methylene blue. The concentration of the surfactant is then determined by measuring the color intensity in the organic phase using a smartphone. Under optimized conditions, an aliquot of 3 mL of the NADES was mixed with 15 mL of water, and then allowed to settle (to enable the separation of the two phases) for 5 min. The procedure allowed quantification of sodium dodecyl sulfate (SDS), as a proxy for alkyl surfactants in the range from 0.010 mg L<small><sup>−1</sup></small> to 0.600 mg L<small><sup>−1</sup></small>, with a detection limit of 2.0 μg L<small><sup>−1</sup></small>. Besides being a simple alternative to the traditional method (which requires chloroform and a spectrophotometer), the proposed approach offers low waste generation, low power-consumption, and fast analysis time, and is fully compatible with the plastic supplies (<em>e.g.</em> cuvettes, pipettes, tips, <em>etc.</em>) typically used for on-site analysis. The applicability of the approach was demonstrated by measuring various surface water samples and the overall green score of the method was calculated to be 96%.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1467-1475"},"PeriodicalIF":3.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00196f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Highly efficient WS2 QD-based non-enzymatic fluorescent biosensor for ofloxacin and ciprofloxacin monitoring in aquatic media† 基于 WS2QDs 的高效非酶促荧光生物传感器用于监测水生介质中的氧氟沙星和环丙沙星
IF 3.5
Sensors & diagnostics Pub Date : 2024-07-24 DOI: 10.1039/D4SD00148F
Sunayana Bora and Chandan Upadhyay
{"title":"Highly efficient WS2 QD-based non-enzymatic fluorescent biosensor for ofloxacin and ciprofloxacin monitoring in aquatic media†","authors":"Sunayana Bora and Chandan Upadhyay","doi":"10.1039/D4SD00148F","DOIUrl":"10.1039/D4SD00148F","url":null,"abstract":"<p >Quantum dot-based biosensors have gained prominence in recent times for the detection of biological and chemical hazards present in aquatic media which essentially contribute to the degradation of the environment and human health. Within this work, we demonstrate a WS<small><sub>2</sub></small> QD-induced turn-on fluorescent probe for specific monitoring of ofloxacin (OFL) and ciprofloxacin (CIP) residues in water. An efficient one-pot hydrothermal approach is applied for fluorescent water-soluble WS<small><sub>2</sub></small> QD preparation. The WS<small><sub>2</sub></small> QDs possess excellent photostability and monodispersity along with a superior shelf life. The WS<small><sub>2</sub></small> QDs interacting with FQns (OFL and CIP) showed a systematically enhanced fluorescence in varying FQn concentrations from 0 μM to 3 μM. Also, all the measurements showed excellent results for sensitivity along with superior specificity as well as anti-interference ability over other interfering substances like various metal ions and antibiotic derivatives. The proposed sensor allows the quantification of FQns in the range of 0–3 μM with the lowest detectable amount (LOD) of 0.08 μM and 0.06 μM and the minimal limit of quantification (LOQ) of 0.26 μM and 0.21 μM for both OFL and CIP, respectively, at natural pH. It achieved higher sensitivity than many established techniques and materials making up the gap of other existing systems in this range. We observed excellent results for the rapid <em>in situ</em> detection of FQns by implementing WS<small><sub>2</sub></small> QDs. The findings show potential for future use in real-time applications for FQns.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1522-1532"},"PeriodicalIF":3.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00148f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A handheld laser-cut device for the size-controlled assembly and electrical characterisation of lipid bilayers† 用于控制脂质双分子层的尺寸和电学特性的手持式激光切割装置
IF 3.5
Sensors & diagnostics Pub Date : 2024-07-24 DOI: 10.1039/D4SD00076E
Ji Huang, Yuval Elani and Mark S. Friddin
{"title":"A handheld laser-cut device for the size-controlled assembly and electrical characterisation of lipid bilayers†","authors":"Ji Huang, Yuval Elani and Mark S. Friddin","doi":"10.1039/D4SD00076E","DOIUrl":"10.1039/D4SD00076E","url":null,"abstract":"<p >We report the rapid fabrication of a handheld laser cut platform that can support the assembly, functionalisation, size-control and electrical characterisation of lipid bilayers. We achieve this by building a modular DIY platform that can support the lowering of a Ag/AgCl electrode through a phase transfer column consisting of an upper oil phase containing lipids, and a lower aqueous phase containing buffer.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1461-1466"},"PeriodicalIF":3.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00076e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA walker coupled with nicking endonuclease for sensitive electrochemical detection of saxitoxin† 与核酸内切酶联用的 DNA 步行器用于灵敏的沙西毒素电化学检测
IF 3.5
Sensors & diagnostics Pub Date : 2024-07-24 DOI: 10.1039/D4SD00167B
Yiwei Liu, Shumin Feng, Ruoxi Zhong, Yuanchang Peng, Guoyuan Mu, Jiayi Bai, Wei Chen and Zhan Qu
{"title":"DNA walker coupled with nicking endonuclease for sensitive electrochemical detection of saxitoxin†","authors":"Yiwei Liu, Shumin Feng, Ruoxi Zhong, Yuanchang Peng, Guoyuan Mu, Jiayi Bai, Wei Chen and Zhan Qu","doi":"10.1039/D4SD00167B","DOIUrl":"10.1039/D4SD00167B","url":null,"abstract":"<p >Saxitoxin (STX) as one of the paralytic shellfish toxins has become a serious public health and environmental issue. In this regards, developing highly sensitive and selective biosensors may help find a solution. Herein, a ferrocene (Fc)-labeled DNA walker coupled with nicking endonuclease Nb.BbvCI was used to construct a sensitive electrochemical aptasensor for STX detection. First, an amplified DNA, aptamer and DNA walker formed a sandwich structure on a gold electrode. This structure was disintegrated when STX was added, resulting in the hybridization of the amplified DNA and DNA walker. Thereafter, the DNA walker was activated by Nb.BbvCI to achieve stepwise cleavage of the hybridized amplified DNA. The released Fc-amplified DNA generated an electrochemical signal that decreased linearly with the logarithm value of STX concentration in the range of 1 pM–100 nM with a detection limit of 0.58 pM. Meanwhile, the proposed aptasensor exhibited good selectivity and recovery rate. The DNA walker coupled with the nicking endonuclease provides effective signal amplification for the detection of toxins and fabrication of sensitive aptasensors.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 8","pages":" 1353-1357"},"PeriodicalIF":3.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00167b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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