Non-organic solvent extraction of capsaicinoids from oil combined with fluorescent lateral flow immunoassay strips for on-site identification of illegally recycled waste cooking oil†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Yulian Wang, Yuxiang Wu, Deji Gesang, Zixuan Dong, Zhaoxu Qin, Qingchun Li, Jin Li, Qianxu Zhou and Guoqing Shi
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Abstract

The illicit use of recycled waste cooking oil poses a threat to food safety, yet there is currently a lack of on-site identification methods. This study targets a key component of recycled cooking oil, capsaicinoids, and establishes a rapid detection method for identifying illegally recycled waste cooking oil on-site. The method involves extracting capsaicinoids from the oil using a non-organic solvent extractant, and then detecting it using fluorescent lateral flow immunoassay (LFIA) strips. The preparation conditions of LFIA test strips were first optimized in this study. Subsequently, a 0.02 mol L−1 solution of dimethyl-β-cyclodextrin was optimized as the extractant for capsaicin. The optimized sample extraction conditions involve a sample-to-extractant volume ratio of 1:2 and an extraction time of 1 minute, with a total extraction and detection time not exceeding 15 minutes. This method demonstrates a limit of detection (LOD) for natural capsaicin in oil samples of 0.14 μg kg−1, with a detection range spanning 0.46 to 81 μg kg−1. The method yields recovery rates between 88.76% and 115.79%, with coefficient of variation (CV) values ranging from 1.80% to 13.37%. Cross-reactivity rates for dihydrocapsaicin and synthetic capsaicin exceed 90%, while the impact of common contaminants or additives in other edible oils on detection is minimal. In conclusion, this approach fulfills the technical criteria mandated by the China Food and Drug Administration for distinguishing capsaicin compounds in recycled oil, offering advantages such as simplicity, rapidity, and “green” operation, making it suitable for rapid on-site identification of illegally recycled waste cooking oil.

Abstract Image

非有机溶剂萃取油中辣椒素结合荧光侧流免疫试纸对非法回收废食用油的现场鉴定
废旧地沟油的非法使用对食品安全构成威胁,但目前缺乏现场识别方法。本研究针对回收食用油的关键成分辣椒素,建立了一种现场非法回收废食用油的快速检测方法。该方法包括使用非有机溶剂萃取剂从辣椒油中提取辣椒素,然后使用荧光侧流免疫测定(LFIA)条进行检测。本研究首先对LFIA试纸条的制备条件进行了优化。以0.02 mol L−1的二甲基-β-环糊精溶液作为辣椒素的萃取剂。优化后的样品提取条件为样品与萃取剂体积比为1:2,提取时间为1分钟,总提取和检测时间不超过15分钟。该方法对油中天然辣椒素的检出限为0.14 μg kg - 1,检测范围为0.46 ~ 81 μg kg - 1。回收率为88.76% ~ 115.79%,变异系数为1.80% ~ 13.37%。二氢辣椒素和合成辣椒素的交叉反应率超过90%,而其他食用油中常见的污染物或添加剂对检测的影响很小。综上所述,该方法符合中国食品药品监督管理局对回收油中辣椒素类化合物的鉴别技术标准,具有简单、快速、“绿色”操作等优点,适用于非法回收废食用油的现场快速鉴定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
2.30
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