Human reproduction open最新文献

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Human Reproduction Open, a wild mustang in the prairie of science. 人类生殖开放,科学草原上的一匹野马。
Human reproduction open Pub Date : 2024-05-06 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae022
Edgardo Somigliana, Anja Pinborg, Andrew Williams
{"title":"<i>Human Reproduction Open</i>, a wild mustang in the prairie of science.","authors":"Edgardo Somigliana, Anja Pinborg, Andrew Williams","doi":"10.1093/hropen/hoae022","DOIUrl":"10.1093/hropen/hoae022","url":null,"abstract":"","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 2","pages":"hoae022"},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11074005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140878099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reply: Inaccurate measures of outcomes in the two-sample Mendelian randomization of vitamin D with miscarriage. 回复:维生素 D 与流产的双样本孟德尔随机试验中的结果测量不准确。
Human reproduction open Pub Date : 2024-04-27 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae026
Feng Zhang, Jingtao Huang, Gangting Zhang, Mengyang Dai, Tailang Yin, Chunyu Huang, Jue Liu, Yan Zhang
{"title":"Reply: Inaccurate measures of outcomes in the two-sample Mendelian randomization of vitamin D with miscarriage.","authors":"Feng Zhang, Jingtao Huang, Gangting Zhang, Mengyang Dai, Tailang Yin, Chunyu Huang, Jue Liu, Yan Zhang","doi":"10.1093/hropen/hoae026","DOIUrl":"10.1093/hropen/hoae026","url":null,"abstract":"","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 2","pages":"hoae026"},"PeriodicalIF":0.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inaccurate measures of outcomes in the two-sample Mendelian randomization of vitamin D with miscarriage. 在维生素 D 与流产的双样本孟德尔随机试验中,对结果的测量不准确。
Human reproduction open Pub Date : 2024-04-27 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae025
Qian Yang, Yangbo Sun, Deborah A Lawlor
{"title":"Inaccurate measures of outcomes in the two-sample Mendelian randomization of vitamin D with miscarriage.","authors":"Qian Yang, Yangbo Sun, Deborah A Lawlor","doi":"10.1093/hropen/hoae025","DOIUrl":"10.1093/hropen/hoae025","url":null,"abstract":"","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 2","pages":"hoae025"},"PeriodicalIF":0.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SARS-CoV-2 infection in IVF-conceived early pregnancy and the risk of miscarriage: a matched retrospective cohort study. 试管婴儿早孕期感染 SARS-CoV-2 与流产风险:一项匹配的回顾性队列研究。
Human reproduction open Pub Date : 2024-04-23 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae024
Jian Xu, Di Mao, Chunlin Liu, Ling Sun
{"title":"SARS-CoV-2 infection in IVF-conceived early pregnancy and the risk of miscarriage: a matched retrospective cohort study.","authors":"Jian Xu, Di Mao, Chunlin Liu, Ling Sun","doi":"10.1093/hropen/hoae024","DOIUrl":"10.1093/hropen/hoae024","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;Is SARS-CoV-2 infection in IVF-conceived early pregnancy associated with a higher risk of miscarriage?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;Infection with SARS-CoV-2 during early pregnancy in women conceiving by IVF may not be associated with an increased rate of miscarriage.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;In naturally conceived pregnancies, most findings have shown that SARS-CoV-2 infection does not increase the risk of miscarriage, while some studies have shown that SARS-CoV-2 infection is associated with a higher risk of miscarriage.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;A matched retrospective cohort study was conducted in a tertiary hospital-based reproductive medicine center. The infection group included women who contracted coronavirus disease 2019 (COVID-19) before 20 weeks gestation from 6 December 2022 to 10 January 2023. Each infected woman was matched with three historical control subjects from 1 January 2018 to 31 May 2022.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting methods: &lt;/strong&gt;The infection group was matched with historical control subjects based on female age (±1 year), number of gestational sacs, number of previous miscarriages, BMI (±2 kg/cm&lt;sup&gt;2&lt;/sup&gt;), main causes of infertility, gestational week, and fresh versus frozen embryo transfer.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;A total of 150 pregnant women infected with COVID-19 before 20 weeks of gestation were included in the infection group, which was matched at a 3:1 ratio with 450 historically pregnant controls. There were no significant differences in age, BMI, and endometrial thickness between the two groups. The overall incidence of miscarriage was not significantly different between the infection group and the control group (4.7% versus 5.8%, &lt;i&gt;P&lt;/i&gt; = 0.68). When the infection group was stratified into three subgroups based on the gestational age at the onset of infection (0-7 + 6, 8-11 + 6, and 12-19 + 6 weeks), no significant differences were observed in the incidence of miscarriage between the infection group and the matched control group in any of the subgroups (9.8% versus 13.8%, &lt;i&gt;P&lt;/i&gt; = 0.60; 5.4% versus 4.5%, &lt;i&gt;P&lt;/i&gt; = 1.00; and 1.4% versus 1.9%, &lt;i&gt;P&lt;/i&gt; = 1.00, respectively).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Limitations reasons for caution: &lt;/strong&gt;The major limitation of this study is the relatively small sample size; therefore, caution is suggested when drawing any definitive conclusions. Nonetheless, our study is the largest sample study of the influence of COVID-19 infection on the miscarriage rate in early pregnancy after IVF.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Wider implications of the findings: &lt;/strong&gt;Our findings may provide important insights for reproductive physicians and obstetricians during preconception and early pregnancy counseling.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study funding/competing interests: &lt;/strong&gt;This study was supported by the Natural Science Foundation of Guangdong Province (No. 2023A15","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 2","pages":"hoae024"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11099652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extracellular vesicles secreted by human aneuploid embryos present a distinct transcriptomic profile and upregulate MUC1 transcription in decidualised endometrial stromal cells. 人类非整倍体胚胎分泌的胞外囊泡呈现出独特的转录组特征,并上调蜕膜化子宫内膜基质细胞中的 MUC1 转录。
Human reproduction open Pub Date : 2024-03-12 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae014
Sofia Makieva, Elisa Giacomini, Giulia Maria Scotti, Dejan Lazarevic, Valentina Pavone, Jessica Ottolina, Ludovica Bartiromo, Matteo Schimberni, Marco Morelli, Alessandra Alteri, Sabrina Minetto, Giovanni Tonon, Massimo Candiani, Enrico Papaleo, Paola Viganò
{"title":"Extracellular vesicles secreted by human aneuploid embryos present a distinct transcriptomic profile and upregulate MUC1 transcription in decidualised endometrial stromal cells.","authors":"Sofia Makieva, Elisa Giacomini, Giulia Maria Scotti, Dejan Lazarevic, Valentina Pavone, Jessica Ottolina, Ludovica Bartiromo, Matteo Schimberni, Marco Morelli, Alessandra Alteri, Sabrina Minetto, Giovanni Tonon, Massimo Candiani, Enrico Papaleo, Paola Viganò","doi":"10.1093/hropen/hoae014","DOIUrl":"10.1093/hropen/hoae014","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;Do extracellular vesicles (EVs) secreted by aneuploid human embryos possess a unique transcriptomic profile that elicits a relevant transcriptomic response in decidualized primary endometrial stromal cells (dESCs)?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;Aneuploid embryo-derived EVs contain transcripts of &lt;i&gt;PPM1J&lt;/i&gt;, &lt;i&gt;LINC00561&lt;/i&gt;, &lt;i&gt;ANKRD34C&lt;/i&gt;, and &lt;i&gt;TMED10&lt;/i&gt; with differential abundance from euploid embryo-derived EVs and induce upregulation of &lt;i&gt;MUC1&lt;/i&gt; transcript in dESCs.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;We have previously reported that IVF embryos secrete EVs that can be internalized by ESCs, conceptualizing that successful implantation to the endometrium is facilitated by EVs. Whether these EVs may additionally serve as biomarkers of ploidy status is unknown.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;Embryos destined for biopsy for preimplantation genetic testing for aneuploidy (PGT-A) were grown under standard conditions. Spent media (30 μl) were collected from euploid (n = 175) and aneuploid (n = 140) embryos at cleavage (Days 1-3) stage and from euploid (n = 187) and aneuploid (n = 142) embryos at blastocyst (Days 3-5) stage. Media samples from n = 35 cleavage-stage embryos were pooled in order to obtain five euploid and four aneuploid pools. Similarly, media samples from blastocysts were pooled to create one euploid and one aneuploid pool. ESCs were obtained from five women undergoing diagnostic laparoscopy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting methods: &lt;/strong&gt;EVs were isolated from pools of media by differential centrifugation and EV-RNA sequencing was performed following a single-cell approach that circumvents RNA extraction. ESCs were decidualized (estradiol: 10 nM, progesterone: 1 µM, cAMP: 0.5 mM twice every 48 h) and incubated for 24 h with EVs (50 ng/ml). RNA sequencing was performed on ESCs.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;Aneuploid cleavage stage embryos secreted EVs that were less abundant in RNA fragments originating from the genes &lt;i&gt;PPM1J&lt;/i&gt; (log2fc = -5.13, &lt;i&gt;P&lt;/i&gt; = 0.011), &lt;i&gt;LINC00561&lt;/i&gt; (log2fc = -7.87, &lt;i&gt;P&lt;/i&gt; = 0.010), and &lt;i&gt;ANKRD34C&lt;/i&gt; (log2fc = -7.30, &lt;i&gt;P&lt;/i&gt; = 0.017) and more abundant in &lt;i&gt;TMED10&lt;/i&gt; (log2fc = 1.63, &lt;i&gt;P&lt;/i&gt; = 0.025) compared to EVs of euploid embryos. Decidualization &lt;i&gt;per se&lt;/i&gt; induced downregulation of &lt;i&gt;MUC1&lt;/i&gt; (log2fc = -0.54, &lt;i&gt;P&lt;/i&gt; = 0.0028) in ESCs as a prerequisite for the establishment of receptive endometrium. The expression of &lt;i&gt;MUC1&lt;/i&gt; transcript in decidualized ESCs was significantly increased following treatment with aneuploid compared to euploid embryo-secreted EVs (log2fc = 0.85, &lt;i&gt;P&lt;/i&gt; = 0.0201).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Large scale data: &lt;/strong&gt;Raw data have been uploaded to GEO (accession number GSE234338).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Limitations reasons for caution: &lt;/strong&gt;The findings of the study will require validation utilizing a second cohort of EV samples.&lt;/p&gt;&lt;p&gt;&lt;stro","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 2","pages":"hoae014"},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10980593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140337893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ovarian ferroptosis induced by androgen is involved in pathogenesis of PCOS. 雄激素诱导的卵巢铁变态反应与多囊卵巢综合症的发病机制有关。
Human reproduction open Pub Date : 2024-02-28 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae013
Xinyu Li, Yunying Lin, Xiaoyue Cheng, Guangxin Yao, Jufang Yao, Shuanggang Hu, Qinling Zhu, Yuan Wang, Ying Ding, Yao Lu, Jia Qi, Hanting Zhao, Xuejiao Bian, Yanzhi Du, Kang Sun, Hugo Vankelecom, Yun Sun
{"title":"Ovarian ferroptosis induced by androgen is involved in pathogenesis of PCOS.","authors":"Xinyu Li, Yunying Lin, Xiaoyue Cheng, Guangxin Yao, Jufang Yao, Shuanggang Hu, Qinling Zhu, Yuan Wang, Ying Ding, Yao Lu, Jia Qi, Hanting Zhao, Xuejiao Bian, Yanzhi Du, Kang Sun, Hugo Vankelecom, Yun Sun","doi":"10.1093/hropen/hoae013","DOIUrl":"10.1093/hropen/hoae013","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;Does ovarian ferroptosis play an active role in the development of polycystic ovary syndrome (PCOS)?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;Increased ovarian ferroptosis was present in PCOS ovaries and the inhibition of ferroptosis with ferrostatin-1 (Fer-1) ameliorated polycystic ovary morphology and anovulation.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;Programmed cell death plays a fundamental role in ovarian follicle development. However, the types and mechanisms of cell death involved in the ovary are yet to be elucidated. Ferroptosis is a recently discovered iron-dependent programmed cell death. Impaired iron metabolism and cell death have been observed in women with PCOS, the main cause of anovulatory infertility. Additionally, previous studies reported that an abnormal expression of noncoding RNA may promote ferroptosis in immortalized ovarian granulosa cell lines. However, little is known about whether ovarian ferroptosis is increased in PCOS, and there is insufficient direct evidence for a role of ferroptosis in PCOS, and the underlying mechanism. Moreover, the effect of the inhibition of ferroptosis with Fer-1 in PCOS remains unclear.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;Ferroptosis was evaluated in human granulosa cells (hGCs) from non-PCOS (n = 6-16) and PCOS (n = 7-18) patients. The experimental study was completed &lt;i&gt;in vitro&lt;/i&gt; using primary hGCs from women undergoing IVF. Improvements in PCOS indicators following ferroptosis inhibition with Fer-1 were investigated in a dehydroepiandrosterone (DHEA)-induced PCOS rat model (n = 8 per group).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting methods: &lt;/strong&gt;Ovarian ferroptosis was evaluated in the following ways: by detecting iron concentrations via ELISA and fluorescent probes; measuring malondialdehyde (MDA) concentrations via ELISA; assessing ferroptosis-related protein abundance with western blotting; observing mitochondrial morphology with transmission electron microscopy; and determining cell viability. Primary hGCs were collected from women undergoing IVF. They were treated with dihydrotestosterone (DHT) for 24 h. The effect of DHT on ferroptosis was examined in the presence or absence of small interfering RNA-mediated knockdown of the putative receptor coregulator for signaling molecules. The role of ovarian ferroptosis in PCOS progression was explored &lt;i&gt;in vivo&lt;/i&gt; in rats. The DHEA-induced PCOS rat model was treated with the ferroptosis inhibitor, Fer-1, and the oocytes and metaphase II oocytes were counted after ovarian stimulation. Additionally, rats were treated with the ferroptosis inducer, RSL3, to further explore the effect of ferroptosis. The concentrations of testosterone, FSH, and LH were assessed.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;Increased ferroptosis was detected in the ovaries of patients with PCOS and in rats with DHEA-induced PCOS. Increased concentrations of Fe&lt;sup&gt;2+&lt;/sup&gt; (&lt;i&gt;P","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 2","pages":"hoae013"},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10973940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140320045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blastocyst telomere length predicts successful implantation after frozen-thawed embryo transfer. 囊胚端粒长度可预测冷冻解冻胚胎移植后的成功植入。
Human reproduction open Pub Date : 2024-02-24 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae012
Chun-Wei Chien, Yen-An Tang, Shuen-Lin Jeng, Hsien-An Pan, H Sunny Sun
{"title":"Blastocyst telomere length predicts successful implantation after frozen-thawed embryo transfer.","authors":"Chun-Wei Chien, Yen-An Tang, Shuen-Lin Jeng, Hsien-An Pan, H Sunny Sun","doi":"10.1093/hropen/hoae012","DOIUrl":"10.1093/hropen/hoae012","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;Do embryos with longer telomere length (TL) at the blastocyst stage have a higher capacity to survive after frozen-thawed embryo transfer (FET)?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;Digitally estimated TL using low-pass whole genome sequencing (WGS) data from the preimplantation genetic testing for aneuploidy (PGT-A) process demonstrates that blastocyst TL is the most essential factor associated with likelihood of implantation.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;The lifetime TL is established in the early cleavage cycles following fertilization through a recombination-based lengthening mechanism and starts erosion beyond the blastocyst stage. In addition, a telomerase-mediated slow erosion of TL in human fetuses has been observed from a gestational age of 6-11 weeks. Finally, an abnormal shortening of telomeres is likely involved in embryo loss during early development.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;Blastocyst samples were obtained from patients who underwent PGT-A and FET in an IVF center from March 2015 to May 2018. Digitally estimated mitochondrial copy number (mtCN) and TL were used to study associations with the implantation potential of each embryo.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting and methods: &lt;/strong&gt;In total, 965 blastocysts from 232 cycles (164 patients) were available to investigate the biological and clinical relevance of TL. A WGS-based workflow was applied to determine the ploidy of each embryo. Data from low-pass WGS-PGT-A were used to estimate the mtCN and TL for each embryo. Single-variant and multi-variant logistic regression, decision tree, and random forest models were applied to study various factors in association with the implantation potential of each embryo.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;Of the 965 blastocysts originally available, only 216 underwent FET. While mtCN from the transferred embryos is significantly associated with the ploidy call of each embryo, mtCN has no role in impacting IVF outcomes after an embryo transfer in these women. The results indicate that mtCN is a marker of embryo aneuploidy. On the other hand, digitally estimated TL is the most prominent univariant factor and showed a significant positive association with pregnancy outcomes (&lt;i&gt;P&lt;/i&gt; &lt; 0.01, odds ratio 79.1). We combined several maternal and embryo parameters to study the joint effects on successful implantation. The machine learning models, namely decision tree and random forest, were trained and yielded classification accuracy of 0.82 and 0.91, respectively. Taken together, these results support the vital role of TL in governing implantation potential, perhaps through the ability to control embryo survival after transfer.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Limitations reasons for caution: &lt;/strong&gt;The small sample size limits our study as only 216 blastocysts were transferred. The number was further reduced to 153 blastocysts, where pregnancy outcome","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 2","pages":"hoae012"},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140186467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
No evidence of a causal relationship between miscarriage and 25-hydroxyvitamin D: a Mendelian randomization study. 没有证据表明流产与 25- 羟维生素 D 之间存在因果关系:孟德尔随机研究。
Human reproduction open Pub Date : 2024-02-19 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae011
Feng Zhang, Jingtao Huang, Gangting Zhang, Mengyang Dai, Tailang Yin, Chunyu Huang, Jue Liu, Yan Zhang
{"title":"No evidence of a causal relationship between miscarriage and 25-hydroxyvitamin D: a Mendelian randomization study.","authors":"Feng Zhang, Jingtao Huang, Gangting Zhang, Mengyang Dai, Tailang Yin, Chunyu Huang, Jue Liu, Yan Zhang","doi":"10.1093/hropen/hoae011","DOIUrl":"10.1093/hropen/hoae011","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;Is there a causal relationship between 25-hydroxyvitamin D (25OHD) and miscarriage?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;In this study, little evidence of a causal relationship was found between low serum 25OHD concentration or vitamin D deficiency and the risk of miscarriages.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;Associations between low vitamin D levels and increased risk of miscarriage have been reported, but causality is unclear.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;The latest and largest genome-wide association studies (GWAS) for serum 25OHD concentration (n = 417 580), vitamin D deficiency (426 cases and 354 812 controls), miscarriage (16 906 cases and 149 622 controls), and the number of miscarriages (n = 78 700) were used to explore the causal association between serum vitamin D levels and miscarriage by two-sample Mendelian randomization analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting methods: &lt;/strong&gt;This study was based on summary GWAS results from the FinnGen database and the UK Biobank. The random-effect inverse-variance weighted method was regarded as the primary analysis; MR-Egger, weighted median, weighted mode, simple mode, and MR-pleiotropy residual sum and outlier (MR-PRESSO) were further employed as complementary methods. MR-Egger intercept analysis and MR-PRESSO were employed to test pleiotropy, and Cochran's Q statistic and leave-one-out sensitivity analysis were used to determine the heterogeneity and robustness of the overall estimates, respectively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;There was insufficient evidence of causal associations between serum 25OHD concentration and miscarriage (odds ratio (OR) = 0.995, 95% CI: 0.888 to 1.114, &lt;i&gt;P&lt;/i&gt; = 0.927), or the number of miscarriages (β = -0.004, 95% CI: -0.040 to 0.032, &lt;i&gt;P&lt;/i&gt; = 0.829). Furthermore, little evidence of causality between genetically determined vitamin D deficiency to miscarriage (OR = 0.993, 95% CI: 0.966 to 1.021, &lt;i&gt;P&lt;/i&gt; = 0.624), or the number of miscarriages (β = 0.001, 95% CI: -0.009 to 0.011, &lt;i&gt;P&lt;/i&gt; = 0.828), was observed. The results of the sensitivity analysis were robust, and no significant heterogeneity or horizontal pleiotropy was found.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Limitations reasons for caution: &lt;/strong&gt;This study is limited by the absence of female-specific GWAS data and the limited amount of GWAS data available for this study, as well as the need for caution in generalizing the findings to non-European ethnic groups.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Wider implications of the findings: &lt;/strong&gt;These findings enhance the current understanding of the intricate association between vitamin D and pregnancy outcomes, challenging prevailing beliefs regarding the strong association with miscarriage. The results provide a special perspective that may prompt further exploration and potentially offer insights for guiding future research and informing clinical guidelines pertaining to th","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 2","pages":"hoae011"},"PeriodicalIF":0.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10918637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140061460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reply: A paradox? Which paradox? 答复:悖论?哪个悖论?
Human reproduction open Pub Date : 2024-02-15 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae007
Edgardo Somigliana, Alessandra Chinè, Marco Reschini, Gianfranco Fornelli, Ludovica Basili, Andrea Busnelli, Paola Viganò, Ludovico Muzii
{"title":"Reply: A paradox? Which paradox?","authors":"Edgardo Somigliana, Alessandra Chinè, Marco Reschini, Gianfranco Fornelli, Ludovica Basili, Andrea Busnelli, Paola Viganò, Ludovico Muzii","doi":"10.1093/hropen/hoae007","DOIUrl":"10.1093/hropen/hoae007","url":null,"abstract":"","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 1","pages":"hoae007"},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10879745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neurotrophin-4 promotes in vitro development and maturation of human secondary follicles yielding metaphase II oocytes and successful blastocyst formation. 神经营养素-4 可促进人类次级卵泡的体外发育和成熟,使卵母细胞分裂期达到 II 期,并成功形成囊胚。
Human reproduction open Pub Date : 2024-01-30 eCollection Date: 2024-01-01 DOI: 10.1093/hropen/hoae005
Yingchun Guo, Lei Jia, Haitao Zeng, Peng Sun, Wenlong Su, Tingting Li, Xiaoyan Liang, Cong Fang
{"title":"Neurotrophin-4 promotes <i>in vitro</i> development and maturation of human secondary follicles yielding metaphase II oocytes and successful blastocyst formation.","authors":"Yingchun Guo, Lei Jia, Haitao Zeng, Peng Sun, Wenlong Su, Tingting Li, Xiaoyan Liang, Cong Fang","doi":"10.1093/hropen/hoae005","DOIUrl":"10.1093/hropen/hoae005","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;Does a matrix-free culture system supplemented with neurotrophic factor 4 (NT4) improve human &lt;i&gt;in vitro&lt;/i&gt; follicular development and meiotic maturation, ultimately resulting in fertilizable oocytes?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;NT4 supplementation of &lt;i&gt;in vitro&lt;/i&gt; culture significantly enhances the growth, steroid hormone production, and maturity potential of human secondary follicles derived from fresh ovarian medulla (from post- and pre-pubertal patients), thereby yielding fertilizable oocytes.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;Reconstituting folliculogenesis &lt;i&gt;in vitro&lt;/i&gt; is of paramount importance in the realms of fertility preservation, reproductive biology research, and reproductive toxicity assessments. However, the efficiency of &lt;i&gt;in vitro&lt;/i&gt; culture systems remains suboptimal, as the attainment of fertilizable oocytes from &lt;i&gt;in vitro&lt;/i&gt; growth (IVG) of human follicles remains unachieved, with the data being particularly scant regarding follicles from prepubertal girls. We have previously found that mouse oocytes from secondary follicles derived from IVG are deficient in neuroendocrine regulation. NT4 and its corresponding receptor have been identified in human follicles. Significantly, the addition of NT4 during the IVG process markedly enhances both follicle growth and oocyte maturation rates in mice.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;Fresh medulla tissue obtained during tissue preparation for ovarian tissue cryopreservation (OTC) were collected from 10 patients aged from 6 to 21 years old, all of whom had undergone unilateral oophorectomy as a means of fertility preservation. Isolated secondary follicles were individually cultured &lt;i&gt;in vitro&lt;/i&gt; with or without NT4 in a matrix-free system.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting methods: &lt;/strong&gt;Secondary follicles, extracted via enzymatic digestion and mechanical disruption from each patient, were randomly allocated to either a control group or an NT4-supplemented group (100 ng/ml), followed by individual culture on an ultra-low attachment plate. Follicle growth and viability were assessed by microscopy. Levels of anti-Müllerian hormone (AMH), estradiol, and progesterone in the medium were quantified. An oocyte-specific marker was identified using confocal fluorescence microscopy following DEAD box polypeptide 4 (DDX4) staining. The competence of individual oocytes for maturation and fertilization were assessed after IVM and ICSI with donated sperm samples.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;Overall, isolated follicles from both groups survived up to 6 weeks with increasing diameters over the duration (&lt;i&gt;P&lt;/i&gt; &lt; 0.05), reaching terminal diameters of almost 1 mm with confirmed steroidogenesis and expression of oocyte marker (DDX4), and producing morphologically normal MII oocytes. When compared with the control group, the NT4 group had a similar initial follicular dia","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2024 1","pages":"hoae005"},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10873269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139901139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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