Human reproduction open最新文献

{"title":"Do different male infertility factors impact embryological, cumulative pregnancy and neonatal outcomes in IVF/ICSI cycles? A retrospective cohort study.","authors":"Liu Jiang, Jiayin Zhou, Haoming Huang, Yan Li, Mingwei Lv, Yueping Zhou, Yuchen Gong, Xinyao Hu, Jie Li, Zhiqi Liao, Xiujuan Tan, Lei Jin, Kun Qian","doi":"10.1093/hropen/hoaf073","DOIUrl":"10.1093/hropen/hoaf073","url":null,"abstract":"<p><strong>Study question: </strong>What are the impacts of different male infertility factors on embryological, cumulative pregnancy and neonatal outcomes of IVF/ICSI cycles?</p><p><strong>Summary answer: </strong>Some severe male infertility factors, i.e. severe oligoasthenozoospermia (OAT-S) and non-obstructive azoospermia (NOA), may be negatively associated with fertilization, embryo development, and cumulative live birth rates, but not with neonatal outcomes.</p><p><strong>What is known already: </strong>Previous studies examining the effect of male infertility factors on IVF/ICSI clinical outcomes have drawn contradictory conclusions, largely because the semen quality of male partners could fluctuate due to many factors, and there are many confounding factors from female partners.</p><p><strong>Study design size duration: </strong>This retrospective cohort study involved 4714 males with various semen abnormalities and 10 283 males with normozoospermia whose partners underwent their first IVF/ICSI cycle between January 2018 and September 2022 in the reproductive medicine centre of a university hospital.</p><p><strong>Participants/materials setting methods: </strong>Only couples with infertility caused by fallopian tubal factors, male factors, or unknown reasons were included. The patients were divided into five different groups: normozoospermia (N), mild-moderate male factor (MMF), OAT-S, azoospermia-husband (Azoospermia-H), and azoospermia-donor (Azoospermia-D). The Azoospermia-H group was further divided into obstructive azoospermia (OA) and NOA. We compared rates of fertilization, embryo development, and cumulative pregnancy as well as neonatal outcomes. Reproductive and neonatal outcomes of men with various semen abnormalities were studied through propensity score matching (PSM) comparisons along with corresponding control groups (N) (with matching factors: female age, female BMI, male age, male BMI, ovarian stimulation protocol, number of oocytes obtained, and endometrial thickness). Fertilization outcomes were also compared and stratified by IVF or ICSI.</p><p><strong>Main results and the role of chance: </strong>The mean female ages in the azoospermia, OAT-S, MMF, and N groups were 28.9, 29.4, 31.0, and 31.0 years old, respectively, which were similar between groups after PSM. The normal fertilization rates were significantly reduced in the OAT-S and Azoospermia-H groups compared with the control group in ICSI cycles (68.1% vs 71.5%, <i>P </i>= 0.001; 65.3% vs 72.4%, <i>P </i>< 0.001). The embryo utilization rates were also significantly decreased in the OAT-S and Azoospermia-H groups compared with controls in IVF/ICSI cycles (48.8% vs 57.3%, <i>P </i>< 0.001; 53.9% vs 58.1%, <i>P </i>= 0.001). Regarding pregnancy outcomes, the cumulative live birth rate in the OAT-S group was decreased (66.3% vs 74.5%, OR 0.68, 95% CI: 0.56-0.81). Among azoospermia cases, the NOA group exhibited a lower live birth rate (66.4% vs 75.8%, OR 0.63, 95% C","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2025 4","pages":"hoaf073"},"PeriodicalIF":11.1,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12701804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145758483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Timing and tempo of pubertal development and substance use in adolescence: a cohort study in the Danish National Birth Cohort.","authors":"Pernille Jul Clemmensen, Nis Brix, Anne Gaml-Sørensen, Anne Marie Ladehoff Thomsen, Thea Emily Benson, Andreas Ernst, Katrine Strandberg-Larsen, Cecilia Høst Ramlau-Hansen","doi":"10.1093/hropen/hoaf072","DOIUrl":"10.1093/hropen/hoaf072","url":null,"abstract":"<p><strong>Study question: </strong>Are the age at reaching different pubertal milestones, and the tempo of pubertal progression, associated with cigarette smoking, alcohol intake, or recreational drug use in adolescence?</p><p><strong>Summary answer: </strong>Earlier age at pubertal development was associated with a higher risk of smoking cigarettes, drinking alcohol (only boys), and using recreational drugs in adolescence, and a faster tempo tended to be associated with higher risks, mainly in girls, while any associations in boys appeared less clear.</p><p><strong>What is known already: </strong>Girls and boys with an earlier age at pubertal development are suggested to have a higher risk of engaging in risk-taking behaviours, which include use of substances, primarily studied for smoking and alcohol. The potential impact of pubertal tempo has been studied less.</p><p><strong>Study design size duration: </strong>In this cohort study, 8063 girls and boys born from 2000 to 2003 by mothers in the Danish National Birth Cohort participated. Information on covariates was obtained from pregnancy interviews and a follow-up questionnaire at age 7. Information on pubertal development was obtained from questionnaires completed by the girls and boys throughout puberty, starting at age 11, and information on substance use, covering cigarette smoking, alcohol, and recreational drug use, was obtained from a questionnaire completed at a mean age of 18 years and 4 months.</p><p><strong>Participants/materials setting methods: </strong>A multinomial logistic regression model was used to obtain adjusted relative risk ratios (RRRs) of smoking, drinking alcohol, and using recreational drugs (non-medical use of psychoactive substances) per 1 year earlier timing of pubertal development (Tanner stages of breast/genital and pubic hair development, menarche, first ejaculation, and voice break) and for tempo per one-unit (Tanner stages per year) faster tempo. Additionally, we also analysed categories of pubertal timing (15% earliest, 70% (ref.), 15% latest) and tempo (15% fastest, 70% (ref.), 15% slowest). The timing and tempo of the development of Tanner stages were derived using a non-linear mixed-effects model.</p><p><strong>Main results and the role of chance: </strong>At the age of 18 years, 19% of girls and 20% of boys reported smoking at least monthly, 69% of girls and 71% of boys reported consuming alcohol more frequently than once a month, and 17% of girls and 30% of boys reported use of recreational drugs (primarily hash or pot) at some point during the past year. In girls, the RRR per 1-year earlier breast development was 1.20 (95% CI, 1.08-1.34) for smoking daily or weekly and 1.10 (95% CI, 0.97-1.25) for smoking monthly, and in boys, the RRR per 1-year earlier genital development was 1.09 (95% CI, 0.94-1.27) for smoking daily or weekly and 1.17 (95% CI, 1.02-1.34) for smoking monthly compared to no smoking. In girls, earlier age at menarche, but no other mil","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2025 4","pages":"hoaf072"},"PeriodicalIF":8.6,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12976676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147446112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mental health of couples affects fertility, modified by socioeconomic status: a couple-based analysis.","authors":"Bingjie Wu, Xiaoyue Cheng, Ruimin Zheng, Hua Yang, Mi Xiang, Wei Qiu, Shuai Yang, Kadila Abulaiti, Jiahao Wu, Wenjuan Wang, Fan Jiang, Jinsong Zhang, Jun Zhang","doi":"10.1093/hropen/hoaf071","DOIUrl":"10.1093/hropen/hoaf071","url":null,"abstract":"<p><strong>Study question: </strong>Does preconception mental health status in either partner affect fertility and infertility, and is this association modified by socioeconomic status (SES)?</p><p><strong>Summary answer: </strong>Preconception mental health problems in both partners are associated with lower couple fertility, with the synergistic impact being most pronounced among couples with low SES status.</p><p><strong>What is known already: </strong>Mental health problems are rising among young adults, and fertility rates are declining. Women's preconception mental health has been linked to lower fertility, but few studies have examined the combined impact of both partners' mental health. The modifying role of SES in these associations is also poorly understood.</p><p><strong>Study design size duration: </strong>This couple-based prospective cohort study included 966 preconception couples who sought preconception care and were followed for 12 months in the Shanghai Birth Cohort between 2013 and 2015.</p><p><strong>Participants/materials setting methods: </strong>The couples' mental health status was evaluated at enrolment using the Center for Epidemiological Studies-Depression Scale, Zung Self-Rating Anxiety Scale, and Perceived Stress Scale. The outcomes included couple fecundability (measured by the TTP) and infertility (i.e. TTP >12 menstrual cycles). In the partner-specific model, Cox proportional hazards models and logistic regression were used to evaluate the associations between each partner's depression, anxiety, and stress levels and couples' fertility. In the couple-based model, cross-classification and quantile g-computation were first applied to identify couples' joint exposure to specific psychological conditions in relation to fertility. Latent profile analysis (LPA) was then conducted to characterize distinct latent profiles of couples' overall mental health statuses, followed by Cox proportional hazards models and logistic regression to examine the corresponding associations. Key symptoms in the couples' depression, anxiety, and stress scales were determined by elastic net regression and least absolute shrinkage and selection operator. To assess the potential effect modification of SES on the association between couples' mental health and fertility, we conducted stratified analyses by male and female partner education levels and household income.</p><p><strong>Main results and the role of chance: </strong>In the female partner-specific model, a 1 SD increase in depression score was associated with 10% lower fecundability (FOR = 0.90, 95% CI: 0.82, 0.99). Likewise, a 1 SD increase in the stress score was associated with 13% lower fecundability (FOR = 0.87, 95% CI: 0.79, 0.96). Male anxiety was associated with a higher risk of infertility (OR = 1.19, 95% CI: 1.01, 1.42). Stratified analyses showed that depression, anxiety, and stress were significantly associated with lower fecundability among males with an education level <co","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2025 4","pages":"hoaf071"},"PeriodicalIF":8.6,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12976685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147446057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroid cancer treatment among adolescents and young adult women and reproductive outcomes: a population-based cohort study.","authors":"Haris Imsirovic, Harriet Richardson, Jonas Shellenberger, Maria P Velez","doi":"10.1093/hropen/hoaf070","DOIUrl":"10.1093/hropen/hoaf070","url":null,"abstract":"<p><strong>Study question: </strong>Are different types of thyroid cancer treatment among young women associated with adverse reproductive outcomes?</p><p><strong>Summary answer: </strong>All types of thyroid cancer treatment were associated with infertility diagnosis and early menopause, but not associated with premature ovarian insufficiency (POI) or lower childbirth rates.</p><p><strong>What is known already: </strong>Thyroid cancer and/or its treatment may affect thyroid function, and thyroid hormone imbalances may affect reproduction.</p><p><strong>Study design size duration: </strong>Population-based matched cohort study included adolescent and young adult women (AYAs, 15-39 years) treated for thyroid cancer in Ontario, Canada between 1992 and 2019, after they had lived at least 3 years free of recurrence.</p><p><strong>Participants/materials setting methods: </strong>Each participant was matched to five cancer-free women based on age, census subdivision, parity, and year of cancer diagnosis. The study cohort consisted of 6474 women undergoing thyroid cancer treatment and 31 922 women without cancer. Exposure status was determined by thyroid cancer treatment, namely: (i) cancer-free (unexposed; referent), (ii) less than total thyroidectomy (LTT), (iii) total thyroidectomy (TOT), or (iv) total thyroidectomy in combination with radioactive iodine therapy (TOT+RAI). Among the exposed, 3396 (52.5%) received TOT, 1520 had (23.5%) LTT, and 1558 (24.1%) had TOT+RAI. Our main outcomes of interest were infertility diagnosis, POI (i.e. cessation of ovarian function before age 40 years), early menopause (menopause before age 45 years), and childbirth rates. Poisson regression models generated weighted relative risks (wRR) using inverse probability of treatment weighting to adjust for imbalances in baseline characteristics.</p><p><strong>Main results and the role of chance: </strong>Mean (SD) age at thyroid cancer diagnosis was 30.6 (6.1) years. The rate of infertility was 3186/31 922 (10.0%) among unexposed, 177/1520 (11.6%) after LTT, 414/3396 (12.2%) after TOT, and 213/1558 (13.7%) following TOT+RAI. The weighted relative risk (wRR) was 1.26 (1.12-1.39) for LTT, 1.22 (1.13-1.32) for TOT, and 1.34 (1.19-1.48) following TOT+RAI. The rate of early menopause was 713/31 922 (2.2%) among unexposed, 46/1520 (3.0%) after LTT, 78/3396 (2.3%) after TOT, and 54/1558 (3.5%) following TOT+RAI. The wRR was 1.42 (1.09-1.72) for LTT, 1.02 (0.83-1.20) for TOT, and 1.54 (1.21-1.89) following TOT+RAI. The rates of POI and childbirth were similar between the unexposed and treatment groups.</p><p><strong>Limitations reasons for caution: </strong>Misclassification is a possibility when using linkage of administrative databases. Absence of information about thyroid hormone supplementation and TSH levels in the study databases is another limitation. Since the unexposed group consisted of cancer-free women, we cannot distinguish whether the observed associations reflect the","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2025 4","pages":"hoaf070"},"PeriodicalIF":11.1,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12671970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145672929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Pregnancy and perinatal outcomes after modified natural cycle-frozen embryo transfers according to size of the dominant follicle on the hCG trigger day.","authors":"","doi":"10.1093/hropen/hoaf066","DOIUrl":"10.1093/hropen/hoaf066","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1093/hropen/hoaf047.].</p>","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2025 4","pages":"hoaf066"},"PeriodicalIF":11.1,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12596473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145491026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Protocol for developing a core outcome set for male infertility research: an international consensus development study.","authors":"","doi":"10.1093/hropen/hoaf065","DOIUrl":"https://doi.org/10.1093/hropen/hoaf065","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1093/hropen/hoac014.].</p>","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2025 4","pages":"hoaf065"},"PeriodicalIF":11.1,"publicationDate":"2025-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12582369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and minimally invasive preimplantation genetic testing for aneuploidies (PGT-A) based on polar body and nanopore sequencing: a viable alternative to conventional trophectoderm-based PGT-A?","authors":"Di Song, Taoli Ding, Tuan Li, Peng Zhang, Yangyun Zou, Yuanbo Hu, Hong Ye, Yajun Xu, Shengnan Wang, Tuanping Zhou, Sijia Lu, Hongli Yan","doi":"10.1093/hropen/hoaf069","DOIUrl":"10.1093/hropen/hoaf069","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;Can third-generation sequencing (TGS)-based polar body (PB) analysis serve as a viable alternative to conventional trophectoderm (TE) biopsy for preimplantation genetic testing for aneuploidy (PGT-A)?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;This study demonstrates the feasibility of using TGS and PB biopsy for clinical PGT-A, particularly in advanced maternal age (AMA) patients.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;TE biopsy, the current standard approach for PGT-A, is limited by embryonic mosaicism. Mosaicism potentially leads to false-positive aneuploidy diagnoses, resulting in the discard of genetically normal embryos and compromising the cumulative live birth rates (CLBRs).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;A total of 125 oocytes were collected from 30 couples. First (PB1) and second (PB2) polar bodies from 89 oocytes were individually amplified and sequenced, while those from the remaining 36 oocytes were processed jointly (PB1 + PB2). Then, 74 oocytes were successfully fertilized and developed into blastocysts (59.2% blastulation rate), and from these, corresponding TE biopsies were obtained.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting methods: &lt;/strong&gt;Both PB and TE samples underwent whole-genome amplification (WGA) using multiple annealing and looping-based amplification cycles (MALBAC), followed by next-generation sequencing (NGS; all samples) and TGS (PB samples only). Copy number variation (CNV) analysis was performed for aneuploidy screening. Single-nucleotide polymorphisms (SNPs) from TE samples and parental peripheral blood were analyzed to determine the origin of CNVs and investigate discrepancies between TE- and PB-based PGT-A results. Clinical information from patients was collected for inter-group statistical comparisons.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;The amplification success rate was 97.75% (87/89) for PB1 and 92.13% (82/89) for PB2, while the amplification success rate for PB1 + PB2 was 97.22% (35/36), comparable to that of TE-biopsied cells. The concordance rate of CNV results between NGS and TGS was 96.81%, with observed discrepancies primarily attributed to differences in the sizes of segmental imbalances and varying levels of intermediate copy numbers. However, when inferring oocyte ploidy status from PB analysis, the concordance rate with TE-biopsied CNV results (blastocyst formation rate 59.2%) was 75.68% (56/74). Among the 56 embryos with consistent results, the CNV profiles of 40 embryos were identical, while the remaining 16 were embryos with paternal meiotic or mitotic abnormalities. Our results demonstrated a higher euploidy rate with PB-based PGT-A (55.4%) compared to blastocyst-stage PGT-A (43.1%). Additionally, the euploidy rate in oocytes from AMA patients (&gt;38 years) was 40%, which was lower than that in younger patients (≤38 years; 64%).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Limitations reasons for caution: &lt;/strong&gt;PGT-A via PB biopsy is su","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2025 4","pages":"hoaf069"},"PeriodicalIF":11.1,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12627406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145566310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Return rates for the use of ovarian tissue cryopreserved prior to gonadotoxic treatment as fertility preservation: a systematic review.","authors":"Anna Mathilde Yde, Lotte Berdiin Colmorn, Amalie Somuncu Johansen, Elisabeth Clare Larsen, Anja Pinborg, Kirsten Tryde Macklon","doi":"10.1093/hropen/hoaf068","DOIUrl":"10.1093/hropen/hoaf068","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;What is the return rate for the use of ovarian tissue cryopreserved for fertility preservation prior to gonadotoxic treatment?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;The return rate for the use of ovarian tissue cryopreserved for fertility preservation prior to gonadotoxic treatment is modest, with most studies reporting rates of ≤5%.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;A considerable number of years have passed since ovarian tissue cryopreservation (OTC) was first implemented for fertility preservation, and many studies now provide long-term follow-up data, including return rates. This allows for a more comprehensive evaluation of OTC as a fertility preservation option and of the characteristics of the population to whom it is offered.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;We searched PubMed, EMBASE, and the Cochrane Library for MeSH words, Emtree terms, and text words related to return rates for the use of ovarian tissue cryopreserved prior to gonadotoxic treatment up to March 2025, in the English language. There were no limitations regarding the year of publication.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting methods: &lt;/strong&gt;Each study was screened independently by two reviewers who were blinded to each other's choices. We included studies reporting the proportion of women who returned for autologous ovarian tissue transplantation (AOTT) among those who had undergone OTC. We excluded studies in languages other than English, abstracts without full text, letters to the editor, and case series involving fewer than 25 females undergoing OTC. Risk of bias was assessed using the Joanna Briggs Institute (JBI) Critical Appraisal Checklist for Cohort Studies. The overall certainty of evidence was assessed using GRADE (grading of recommendations, assessment, development, and evaluation).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;Out of 2748 studies, 25 were included in the review. All studies were cohort studies that included from 26 to 2475 participants. Of the 25 studies, 18 reported a return rate of ≤5%, 6 reported a return rate of &gt;5 to ≤10% while only 1 study reported a return rate of 14%. The overall quality of the studies in reporting the return rate for the use of cryopreserved ovarian tissue, as assessed using GRADE, was moderate.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Limitations reasons for caution: &lt;/strong&gt;In most studies, follow-up occurred within the overall study period, and only one study reported a minimum follow-up duration. Since AOTT may occur several years after OTC, limited follow-up time may bias the results. Most studies included both pediatric patients and adults undergoing OTC, without accounting for participants who had not yet reached reproductive age or desired pregnancy when calculating return rates at the end of follow-up.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Wider implications of the findings: &lt;/strong&gt;The modest utilization rates found in this study emphasize the need for careful consider","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2025 4","pages":"hoaf068"},"PeriodicalIF":11.1,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12638063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145590030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lateral distribution of endometriotic lesions: the anatomical recesses hypothesis. A systematic review and meta-analysis.","authors":"Veronica Bandini, Sonia Cipriani, Chiara Pillinini, Stefano Angioni, Ludovica Bartiromo, Anna Biasioli, Massimo Candiani, Valerio Carletti, Maurizio Nicola D'Alterio, Domenico Incandela, Lucia Lazzeri, Antonio Maiorana, Ludovico Muzii, Alessio Perandini, Federica Perelli, Maria Grazia Porpora, Diego Raimondo, Valentino Remorgida, Giulia Rovero, Federica Savasta, Stefano Scarperi, Matteo Schimberni, Renato Seracchioli, Eugenio Solima, Giuseppe Sorrenti, Roberta Venturella, Michele Vignali, Giuseppe Vizzielli, Veronica Yacoub, Fulvio Zullo, Errico Zupi, Paolo Vercellini","doi":"10.1093/hropen/hoaf064","DOIUrl":"10.1093/hropen/hoaf064","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;Are endometriotic lesions affecting bilateral organs or anatomical structures distributed symmetrically on both sides of the body?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;The left-sided preponderance of pelvic endometriotic lesions, and the right-sided dominance of thoracic and inguinal lesions, suggest that endometriotic lesions exhibit a non-random, asymmetric lateral distribution.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;Evaluating the anatomical distribution of endometriotic lesions may elucidate the underlying pathogenic mechanism(s) of the disease. If the coelomic metaplasia or embryonic cell remnant theory is correct, a symmetrical right-left pattern would be expected. Conversely, retrograde menstruation would likely result in asymmetrical distribution, influenced by gravity, peritoneal fluid circulation, and anatomical niches.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;This systematic review with meta-analysis included full-length, English-language articles published up to 10 June 2024. Literature searches were performed in PubMed/Medline and Embase databases with the keyword 'endometriosis', 'lateral', 'distribution', 'right', 'left', and 'asymmetry'.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting methods: &lt;/strong&gt;The review focused on anatomical structures commonly affected by endometriosis with surgically defined right or left laterality: ovaries, uterosacral ligaments, colon, ureters, inguinal regions, and hemithorax (diaphragm, pleura, lungs). Case reports were excluded. Risk of bias was assessed using ROBINS-I for non-randomized studies and a dedicated tool for case series. Meta-analyses of proportions were conducted in R. Heterogeneity was quantified using the &lt;i&gt;I&lt;/i&gt; &lt;sup&gt;2&lt;/sup&gt; statistic. Funnel plots for publication bias and Egger tests were performed using Stata.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;Of 6356 articles screened, 154 met the inclusion criteria. A statistically significant left-sided preponderance was observed for ovarian (58%; 95% CI: 57-60%; &lt;i&gt;P &lt; &lt;/i&gt;0.001), uterosacral ligament (56%; 95% CI: 54-59%; &lt;i&gt;P &lt; &lt;/i&gt;0.001), ureteral (71%; 95% CI: 67-76%; &lt;i&gt;P &lt; &lt;/i&gt;0.001), and bowel (72%; 95% CI: 64-79%; &lt;i&gt;P &lt; &lt;/i&gt;0.001) lesions, whereas thoracic (98%; 95% CI: 96-100%; &lt;i&gt;P &lt; &lt;/i&gt;0.001) and inguinal (92%; 95% CI: 83-98%; &lt;i&gt;P &lt; &lt;/i&gt;0.001) lesions were predominantly right-sided. These findings were confirmed in the sensitivity analyses. Egger's test indicated a possible small study effect only for ovarian lesions (&lt;i&gt;P &lt;/i&gt;= 0.012).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Limitations reasons for caution: &lt;/strong&gt;The preponderance of retrospective studies, the variability in surgical procedures, and the potential difficulties in accurately distinguishing unilateral from bilateral lesions may have influenced the magnitude of the estimated difference. However, the large patient cohorts, geographical diversity, and consistent asymmetry across lesion types strengthen the resu","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2026 1","pages":"hoaf064"},"PeriodicalIF":11.1,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12816922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146020356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spermatocyte injection into meiotic oocytes rescues diplotene, but not pachytene, arrest in azoospermic mutant mice.","authors":"Narumi Ogonuki, Toshiaki Hino, Yasuhiro Fujiwara, Yuki Osawa, Seiya Mizuno, Fumihiro Sugiyama, Tetsuo Kunieda, Junko Otsuki, Seiya Oura, Tamio Furuse, Yuki Okada, Masaru Tamura, Elena de la Casa-Esperon, Masahito Ikawa, Kimiko Inoue, Atsuo Ogura","doi":"10.1093/hropen/hoaf067","DOIUrl":"10.1093/hropen/hoaf067","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Study question: &lt;/strong&gt;At which arrest stage can spermatocytes be rescued by injection into meiotic oocytes?&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Summary answer: &lt;/strong&gt;In mice, spermatocytes arrested at the diplotene stage, but not at the pachytene stage, can resume meiosis within immature oocytes and support full-term embryonic development.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;What is known already: &lt;/strong&gt;In mice, at least some of the spermatocyte arrest mutations can be overcome by injecting spermatocytes into immature oocytes.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Study design size duration: &lt;/strong&gt;The study was carried out from October 2019 to April 2025. Adult azoospermic mice (at 4-26 weeks of age) from nine strains carrying spermatocyte arrest mutations were used as spermatocyte donors. Adult B6D2F1 females at 9-12 weeks of age were used as oocyte donors for spermatocyte injection. Adult ICR strain pseudopregnant females at 9-12 weeks of age were used as recipients for embryo transfer experiments.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Participants/materials setting methods: &lt;/strong&gt;The most advanced stage of spermatocytes from each mutant strain was assessed by chromosome spread analysis. These most advanced spermatocytes of each strain were injected into metaphase I (MI) oocytes. About half a volume of the ooplasm had been removed from the recipient oocytes to ensure more stable chromosome behaviours during meiosis. The spermatocyte-injected oocytes were allowed to mature &lt;i&gt;in vitro&lt;/i&gt; to the metaphase II (MII) stage, and their ooplasm was refreshed with the ooplasm from intact MII oocytes. After activation with SrCl&lt;sub&gt;2&lt;/sub&gt;, the reconstructed oocytes that reached the 2-cell stage were transferred into the oviducts of pseudopregnant females. On Day 19.5, recipient females were euthanized and their uteri were examined for live foetuses.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Main results and the role of chance: &lt;/strong&gt;Based on spermatocyte spread analysis, sperm mutants were categorized into three classes: Class 1, arrest at mid-diplotene or later stage; Class 2, arrest at early diplotene stage; and Class 3, arrest at pachytene stage. All four Class 1 mutants could resume normal meiosis following injection into MI oocytes, as evidenced by births of normal offspring. Similarly, one of two Class 2 mutants could be rescued, but the other could not. By contrast, three Class 3 mutants did not support embryo development to term because of complete implantation failure, indicating that reconstructed embryos carried severe chromosomal aberrations.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Large-scale data: &lt;/strong&gt;N/A.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Limitations reasons for caution: &lt;/strong&gt;The number of mutant strains examined was limited. Nevertheless, the findings were consistent: the more advanced the arrest stage of spermatocytes, the higher the likelihood of a successful rescue.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Wider implications of the findings: &lt;/strong&gt;In humans, a considerable proportion of spermatogenic arrest occurs at the primary spermatocyte stage. Spermatocyte injection might be an","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":"2025 4","pages":"hoaf067"},"PeriodicalIF":11.1,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12628785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145566363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}