Advanced Therapeutics最新文献_第4页

Advancements in Cancer Research: 3D Models, Single-Cell, and Live-Cell Techniques for Better Insights 癌症研究的进展：3D模型，单细胞和活细胞技术，以获得更好的见解

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-11-09 DOI: 10.1002/adtp.202400351

Federica Carnevali, Stefania Forciniti, Valentina Onesto, Anna Chiara Siciliano, Helena Iuele, Giuliana Grasso, Anderson Fraga da Cruz, Ilaria Serra, Norma Depalma, Stefano D'Ugo, Prisco Piscitelli, Marcello G. Spampinato, Giuseppe Gigli, Carolina Camargo de Oliveira, Loretta L. del Mercato

{"title":"Advancements in Cancer Research: 3D Models, Single-Cell, and Live-Cell Techniques for Better Insights","authors":"Federica Carnevali, Stefania Forciniti, Valentina Onesto, Anna Chiara Siciliano, Helena Iuele, Giuliana Grasso, Anderson Fraga da Cruz, Ilaria Serra, Norma Depalma, Stefano D'Ugo, Prisco Piscitelli, Marcello G. Spampinato, Giuseppe Gigli, Carolina Camargo de Oliveira, Loretta L. del Mercato","doi":"10.1002/adtp.202400351","DOIUrl":"https://doi.org/10.1002/adtp.202400351","url":null,"abstract":"The present review provides a comprehensive overview of the current state of in vitro cancer studies, focusing on recent advancements and ongoing in cell culture models and analyses techniques. Cancer cells grow in a complex and dynamic environment, interacting with various cellular components, such as stromal cells, cancer-associated fibroblasts, immune cells, and the extracellular matrix (ECM). The ECM provides structural support and unique characteristics essential for tumorigenesis. Accurately modeling this intricate tumor microenvironment and precisely analyzing cell–cell and cell–ECM interactions are crucial for understanding cancer progression and therapeutic responses. Consequently, oncology research is advancing toward a) three-dimensional tumor models, b) single-cell level analyses, and c) live-cell analyses. This review aims to elucidate current knowledge in this field, emphasizing the benefits these innovative approaches offer over traditional two-dimensional models, bulk analyses, and endpoint measurements.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"7 12","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adtp.202400351","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142868435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-Assembled Nanocarriers of Synthetic and Natural Plasmalogens for Potential Nanomedicine Development

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-11-06 DOI: 10.1002/adtp.202400093

Yu Wu, Borislav Angelov, Yuru Deng, Takehiko Fujino, Md Shamim Hossain, Thomas Bizien, Angelina Angelova

{"title":"Self-Assembled Nanocarriers of Synthetic and Natural Plasmalogens for Potential Nanomedicine Development","authors":"Yu Wu, Borislav Angelov, Yuru Deng, Takehiko Fujino, Md Shamim Hossain, Thomas Bizien, Angelina Angelova","doi":"10.1002/adtp.202400093","DOIUrl":"https://doi.org/10.1002/adtp.202400093","url":null,"abstract":"Plasmalogens are bioactive glycerophospholipids with a vinyl ether bond at the sn-1 position of the glycerol backbone, which imparts free-radical scavenging properties. Amelioration of the plasmalogen levels, which decline with aging, is required for novel metabolic therapies for Parkinson's disease (PD), Alzheimer's disease (AD), and dementia. This work designed, prepared, and in vitro characterized plasmalogen-loaded lipid nanoparticles with neuroprotective potential for neuronanomedicine. Liquid crystalline nanoparticles formed by natural scallop-derived plasmalogens are in vitro evaluated with respect to synthetic docosapentaenoyl (DPA) plasmenyl (vinyl ether) glycerophospholipids, formulated with the helper lipid monoolein (MO). The structural organization of the lipid nanoparticles is characterized by synchrotron small-angle X-ray scattering (SAXS). The employed self-assembly technique yielded plasmalogen-based nanoassemblies of hexosome, cubosome, vesicular, or intermediate topology types. The internalization of fluorescently-labeled nanoparticles in differentiated human neuroblastoma SH-SY5Y cells is followed by flow cytometry. Under the investigated conditions, the scallop-derived plasmalogen nanocarriers significantly influenced the measured mitochondrial membrane potential compared to those involving synthetic plasmalogens with DPA chains. Levels of protein biomarkers, such as brain-derived neurotrophic factor (BDNF), can be regulated depending on the nanocarrier type. The results identified cubosome, hexosome, and vesicular types of plasmalogen nanocarriers presenting antioxidant properties and preserving neuronal cell integrity and viability.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"8 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adtp.202400093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-Responsive Lipid Nanoparticles for Targeted Therapy and Controlled Drug Release in Non-Small Cell Lung Cancer 超声响应的脂质纳米颗粒用于非小细胞肺癌的靶向治疗和药物释放控制

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-11-05 DOI: 10.1002/adtp.202400248

Min Woo Kim, Sol Moon, Yong Il Park, Jungho Kim, Seung Il Kim, Ruda Lee

{"title":"Ultrasound-Responsive Lipid Nanoparticles for Targeted Therapy and Controlled Drug Release in Non-Small Cell Lung Cancer","authors":"Min Woo Kim, Sol Moon, Yong Il Park, Jungho Kim, Seung Il Kim, Ruda Lee","doi":"10.1002/adtp.202400248","DOIUrl":"https://doi.org/10.1002/adtp.202400248","url":null,"abstract":"Multifunctional drug delivery systems offer tremendous potential for improving antitumor efficacy, precise drug targeting, and controlled drug release in treating non-small cell lung cancer (NSCLC). In this study, the study develops a novel tumor-targeting ultrasound-responsive lipid nanoparticle (TUSL) platform capable of responding to external stimulation, enabling precise drug delivery with controlled release at the tumor site while minimizing systemic exposure and side effects. The TUSL platform is designed to carry doxorubicin (DOX) and tetrandrine (TET), specifically for drug-resistant NSCLC. The developed TUSL exhibits a nano sized spherical structure with a hydrodynamic size of 141.8 nm, accommodating anti-cancer drugs with loading capacities of 3.8% and 6.2% for TET and DOX, respectively. TUSL is engineered to target the epidermal growth factor receptor (EGFR) while demonstrating an ultrasound-triggered drug release profile. Through the generation of CO2 bubbles upon ultrasound stimulation, the TUSL enhances DOX and TET internalization into tumor cells. In tumor-bearing mice, TUSL administration demonstrates a superior tumor accumulation with minimal off-target toxicity. The combined treatment with DOX and TET within TUSL exhibits synergistic effects, effectively inhibiting the growth of drug-resistant NSCLC tumors. These findings highlight the efficacy of the EGFR-targeted TUSL formulation in overcoming drug resistance and enhancing therapeutic outcomes in NSCLC.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"7 12","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploiting Spinach-Derived Extracellular Vesicles for Anti-Obesity Therapy Through Lipid Accumulation Inhibition (Adv. Therap. 11/2024) 利用菠菜提取的细胞外囊泡通过抑制脂质积累抗肥胖（Adv. Therap.）

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-11-05 DOI: 10.1002/adtp.202470024

Jeong Hyun Lee, Su Jin Kang, Won Jong Rhee

引用次数: 0

Issue Information (Adv. Therap. 19/2024) 发行信息（Adv. Therap.）

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-11-05 DOI: 10.1002/adtp.202470025

引用次数: 0

Ex Vivo Modeling of the Tumor Microenvironment to Develop Therapeutic Strategies for Gliomas (Adv. Therap. 11/2024) 建立肿瘤微环境的体内外模型以开发胶质瘤的治疗策略（Adv.）

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-11-05 DOI: 10.1002/adtp.202470026

Philipp Graber, M. Emmy M. Dolman, MoonSun Jung, Maria Kavallaris

引用次数: 0

Nanotechnology-Based Systems for Enhancing the Efficacy of Sonodynamic Therapy in Cancer Treatment 基于纳米技术的系统提高声动力治疗在癌症治疗中的疗效

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-11-05 DOI: 10.1002/adtp.202400309

Yidong Wang, Nikolitsa Nomikou

引用次数: 0

Applications and Developments of Gene Therapy Drug Delivery Systems for Neurological Disorders 神经系统疾病基因治疗药物传递系统的应用与发展

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-11-02 DOI: 10.1002/adtp.202400269

Ngoc Hong Nguyen, Phuong-Trang Nguyen-Thi, Thuy Trang Nguyen, Vu Khac Hoang Bui, Nhat Thang Thi Nguyen, Giau Van Vo

{"title":"Applications and Developments of Gene Therapy Drug Delivery Systems for Neurological Disorders","authors":"Ngoc Hong Nguyen, Phuong-Trang Nguyen-Thi, Thuy Trang Nguyen, Vu Khac Hoang Bui, Nhat Thang Thi Nguyen, Giau Van Vo","doi":"10.1002/adtp.202400269","DOIUrl":"https://doi.org/10.1002/adtp.202400269","url":null,"abstract":"Neurological diseases (NDs) such as Alzheimer's disease, Parkinson's disease, ischemic strokes, spinal cord injuries, and other similar conditions that continue to pose a substantial health and economic burden on a global scale. It is crucial to tackle the difficulties provided by current medications due to the adverse effects and its immunological reactions to develop improved treatments for neurodegenerative illnesses. Gene therapy is currently being extensively used in preclinical and clinical studies for various diseases because of its ability to enhance the delivery and effectiveness of treatments. Various gene delivery techniques, including messenger RNA, small interfering RNA, antisense oligonucleotides, microRNA, CRISPR/Cas9 system, and plasmid DNA, have been created to address these difficulties. The goal of this study is to provide a clear overview of the pathophysiological underpinnings of NDs illnesses while also illuminating recent developments in gene delivery vector technologies. It goes over the main classifications of these vectors, their individual benefits and drawbacks, and their specific applications in the delivery of gene therapy.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"7 12","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142868065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA-Based Nanocarriers to Sequester Altered microRNAs in Cardiac Dysfunction 基于dna的纳米载体在心功能障碍中隔离改变的microrna

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-11-01 DOI: 10.1002/adtp.202400247

Alejandro Postigo, Natalia Hernández-Bellido, Marcos Sánchez-Barat, Laura García-Mendívil, Esther Pueyo, Jesús del Barrio, Silvia Hernández-Ainsa, Laura Ordovás

{"title":"DNA-Based Nanocarriers to Sequester Altered microRNAs in Cardiac Dysfunction","authors":"Alejandro Postigo, Natalia Hernández-Bellido, Marcos Sánchez-Barat, Laura García-Mendívil, Esther Pueyo, Jesús del Barrio, Silvia Hernández-Ainsa, Laura Ordovás","doi":"10.1002/adtp.202400247","DOIUrl":"https://doi.org/10.1002/adtp.202400247","url":null,"abstract":"MicroRNAs (miRs) play a critical role in modulating gene expression across biological processes, including cardiac aging and disease. As such, miRs have demonstrated therapeutic potential in several cardiac conditions. Efficient delivery of miR therapies to cardiac tissue is crucial for effective gene therapy and DNA-based nanocarriers (DNCs), based on Watson-Crick-Franklin highly specific base-pair recognition, have emerged as a promising, biocompatible alternative to viral-based methods. Here, DNCs designed to modulate miR levels as a potential treatment for cardiac dysfunction are presented. Specifically, the DNCs target miR-24-2, which inhibits SERCA2 gene. In humans, the reduction of SERCA2 activity is a hallmark of heart failure and is altered in cardiac aging. The assembled DNCs bearing anti-miR-24-2-5p sequences effectively restore intracellular levels of SERCA2 in a HEK293 cell model. The DNCs proper assembly is thoroughly verified, while their stability and miR-capture ability are demonstrated in vitro. The DNCs exhibit successful internalization into HEK293 and modest uptake into human cardiomyocytes. SERCA2 restoration by DNCs is significantly influenced by the miR-capture sequence layout, underscoring the importance of precise design for optimal biological outcomes. This study highlights the potential of DNCs in cardiac therapies, a previously unexplored avenue for addressing cardiac dysfunction.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"7 12","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adtp.202400247","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142868082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferric-Tannic Nanoparticles Inhibit Early-Stage Hepatocarcinogenesis by Activating Tumor Immune Responses in Rats 铁单宁纳米颗粒通过激活肿瘤免疫反应抑制大鼠早期肝癌的发生

IF 3.7 4区医学

Advanced Therapeutics Pub Date : 2024-10-30 DOI: 10.1002/adtp.202400348

Jannarong Intakhad, Arpamas Vachiraarunwong, Rawiwan Wongpoomchai, Chalermchai Pilapong

引用次数: 0