Genetic Engineering Methods in Primary T Cells

IF 2.6 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Anthony Youssef, Hui-Shan Li
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Abstract

Genetic engineering in primary T cells is gaining traction in the context of gene therapy and cell therapy, with studies aiming to either induce gene expression/correction, gene inhibition, or a combination of both. These genetic modifications can be achieved using a variety of methods, each with its own advantages and limitations. Also, primary T cell genomes can be edited stably, leading to permanent changes, via methods such as lentiviral transduction and CRISPR; and they can also be edited transiently, using tools such as mRNA transfection, to induce only temporary expression or inhibition of genes. While each of these methods possesses their own characteristics that distinguish them from each other, they also face obstacles in their usage in primary T cells. In this review, the principles and mechanisms behind these gene manipulation tools, as well as their advantages and potential limitations, are discussed.

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原代T细胞的基因工程方法
在基因治疗和细胞治疗的背景下,原代T细胞的基因工程正在获得关注,研究旨在诱导基因表达/纠正,基因抑制或两者结合。这些基因修饰可以通过多种方法实现,每种方法都有其自身的优点和局限性。此外,原代T细胞基因组可以通过慢病毒转导和CRISPR等方法进行稳定编辑,从而导致永久性变化;它们也可以被短暂地编辑,使用mRNA转染等工具,只诱导基因的暂时表达或抑制。虽然这些方法都有各自的特点,使它们彼此区别开来,但它们在原代T细胞中的应用也面临障碍。本文综述了这些基因操作工具的原理和机制,以及它们的优势和潜在的局限性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Therapeutics
Advanced Therapeutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.10
自引率
2.20%
发文量
130
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