Consolidation of Ultrasound and Light: Silicon Phthalocyanine-Based Sensitizer Therapeutic Agent for Synergetic Sonodynamic–Photodynamic Therapy of Breast Cancer

IF 2.6 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Hilal Çakmak, Aysegul Turkkol, Umut Kerem Kolac, Göknur Yaşa Atmaca, Aleksey E. Kuznetsov, M. Serdar Çavuş, Mustafa Z. Yıldız, Lukasz Sobotta, Mehmet Dincer Bilgin, Ali Erdoğmuş, Emre Güzel
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Abstract

Sono-photodynamic therapy (SPDT), a useful technique applied in combination with photodynamic therapy (PDT) and sonodynamic therapy (SDT), reduces potential side effects compared to monotherapy. This study reports the photochemical and sono-photochemical properties and in vitro analysis of silicon (IV) phthalocyanine (SiPc), with a particular focus on its efficiency in singlet oxygen production. When photochemical investigations are conducted alone, the SiPc Φ value is measured as 0.68; however, when light and ultrasound are combined, the value increased by 25% to 0.85 in sono-photochemical studies. The Q-band of the calculated SiPc UV–vis spectrum is found to be in very good agreement with the experimental data, with the computed oscillator strengths (the absorption intensities) for Q-band being higher than for B-band. Furthermore, the therapeutic effects of PDT and SPDT using SiPc are evaluated in MCF-7 and MDA-MB-231 breast cancer cell lines. The results demonstrated that SPDT, combining light and ultrasound, significantly enhanced cytotoxicity compared to PDT alone. Additionally, SPDT triggered pyroptosis, characterized by upregulation of NLRP3, CASP1, IL1B, and IL18, revealing a distinct mechanism of cell death. These findings suggest that SiPc-mediated SPDT amplifies oxidative stress and activates multiple cell death pathways, offering a promising and targeted approach for improving breast cancer therapy.

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超声与光的巩固:基于酞菁硅的增敏剂治疗剂用于声动力-光动力协同治疗乳腺癌
声光动力疗法(SPDT)是一种与光动力疗法(PDT)和声动力疗法(SDT)联合应用的有用技术,与单一疗法相比,可以减少潜在的副作用。本研究报道了酞菁硅(SiPc)的光化学和声光化学性质以及体外分析,特别关注了其单线态产氧的效率。单独进行光化学调查时,SiPc Φ∆值测量为0.68;然而,当光和超声相结合时,该值在声光化学研究中增加了25%,达到0.85。计算得到的SiPc紫外可见光谱的q波段与实验数据吻合较好,计算得到的q波段振荡强度(吸收强度)高于b波段。此外,我们还在MCF-7和MDA-MB-231乳腺癌细胞株中评估了SiPc对PDT和SPDT的治疗效果。结果表明,光和超声结合的SPDT与单独的PDT相比,显著增强了细胞毒性。此外,SPDT触发焦亡,其特征是NLRP3、CASP1、IL1B和IL18上调,揭示了细胞死亡的独特机制。这些发现表明sipc介导的SPDT可以放大氧化应激并激活多种细胞死亡途径,为改善乳腺癌治疗提供了一种有希望的有针对性的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Therapeutics
Advanced Therapeutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.10
自引率
2.20%
发文量
130
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